Abstract：
:Common carp thrombocytes account for 30-40% of peripheral blood leukocytes and are abundant in the healthy animals' spleen, the thrombopoietic organ. We show that, ex vivo, thrombocytes from healthy carp express a large number of immune-relevant genes, among which several cytokines and Toll-like receptors, clearly pointing at immune functions of carp thrombocytes. Few studies have described the role of fish thrombocytes during infection. Carp are natural host to two different but related protozoan parasites, Trypanoplasma borreli and Trypanosoma carassii, which reside in the blood and tissue fluids. We used the two parasites to undertake controlled studies on the role of fish thrombocytes during these infections. In vivo, but only during infection with T. borreli, thrombocytes were massively depleted from the blood and spleen leading to severe thrombocytopenia. Ex vivo, addition of nitric oxide induced a clear and rapid apoptosis of thrombocytes from healthy carp, supporting a role for nitric oxide-mediated control of immune-relevant thrombocytes during infection with T. borreli. The potential advantage for parasites to selectively deplete the host of thrombocytes via nitric oxide-induced apoptosis is discussed.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Fink IR,Ribeiro CM,Forlenza M,Taverne-Thiele A,Rombout JH,Savelkoul HF,Wiegertjes GFdoi
10.1016/j.dci.2015.02.008subject
Has Abstractpub_date
2015-06-01 00:00:00pages
146-54issue
2eissn
0145-305Xissn
1879-0089pii
S0145-305X(15)00031-2journal_volume
50pub_type
杂志文章abstract：:We evaluated juvenile, pubescent, reproductive adult, and aged Japanese quail (Coturnix coturnix japonica) to determine if there were age-related differences in immune function with the hypothesis that aged birds would have weaker immune responses. Immune responses were measured using phytohemagglutinin (PHA) skin tes...
journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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