Abstract：
:In crustaceans, lipopolysaccharide- and β-1,3-glucan-binding protein (LGBP) plays an important role in innate immunity by mediating the recognition of pathogens to host cells. Hereby, LGBP was cloned from Fenneropenaeus merguiensis hepatopancreas. Its full-length cDNA (1280 bp) had an open reading frame of 1101 bp, encoding a peptide of 366 amino acids. The LGBP primary structure comprises a recognition motif for β-1,3-linkage of polysaccharides, two integrin binding motifs, a kinase C phosphorylation site and a bacterial glucanase motif. The LGBP mRNA was strongly expressed in hepatopancreas and significantly up-regulated to get the maximum at 12 h upon Vibrio harveyi challenge. Recombinant LGBP (rLGBP) could agglutinate Gram-negative and Gram-positive bacteria including yeast with Ca2+-dependence. V. harveyi agglutination induced by rLGBP was intensively inhibited by lipoteichoic acid, less in order were lipopolysaccharide, β-1,3-glucan and N-acetyl neuraminic acid. Western blotting revealed that rLGBP bound widely to Gram-negative and Gram-positive bacteria and also yeast. By ELISA quantification, rLGBP could bind to β-1,3-glucan better than to lipopolysaccharide and lipoteichoic acid. These findings suggest that LGBP may function as a receptor which recognizes invading diverse pathogens and contribute in F. merguiensis immune response.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Chaosomboon A,Phupet B,Rattanaporn O,Runsaeng P,Utarabhand Pdoi
10.1016/j.dci.2016.07.006subject
Has Abstractpub_date
2017-02-01 00:00:00pages
434-444eissn
0145-305Xissn
1879-0089pii
S0145-305X(16)30223-3journal_volume
67pub_type
杂志文章abstract：:This discussion is on immune response to Amyloodinium ocellatum, Cryptobia salmositica, Trypanoplasma borreli and Trypanosoma carassii. Piscidin and histone-like proteins enhance innate resistance to Amyloodinium. Fish that are naturally resistant to Cryptobia and Trypanoplasma can be bred. Cryptobia resistance in cha...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：10.1016/j.dci.2013.07.006
更新日期：2014-04-01 00:00:00
abstract：:MicroRNAs (miRNAs) have been identified as important post transcriptional regulators of gene expression. In higher vertebrates, a subset of miRNAs has been identified as important regulators of a number of key genes in immune system gene networks, and this paper review recent studies on miRNAs associated with immune r...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：10.1016/j.dci.2017.02.023
更新日期：2017-10-01 00:00:00
abstract：:This review summarizes the scattered literature on the effects of toxicants on the external and internal innate immunity of fish. Insecticides, heavy metals and surfactants have been the most frequently examined toxicants, whereas dioxins, furans and polychlorinated biphenyls have been tested less frequently. Studies ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：10.1016/s0145-305x(01)00040-4
更新日期：2001-10-01 00:00:00
abstract：:The role of accessory cells in the proliferative response of chicken spleen cells to Staphylococcus aureus Cowan I (SAC) was examined. It was found that chicken spleen cells cultured with SAC produced a soluble molecule capable of causing proliferation when culture supernatants were added to spleen cells. The molecule...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(87)90020-6
更新日期：1987-01-01 00:00:00
abstract：:To date, immunologists have operated with two primary paradigms governing the antibody response: (1) that affinity maturation is primarily dependent upon antigen-driven selection of both the germline and somatically amended repertoires, and (2) that antibody effector function is isotypically determined. The teleost mo...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：10.1016/j.dci.2011.01.015
更新日期：2011-09-01 00:00:00
abstract：:The NF-kappaB group of transcription factors play an important role in mediating immune responses in organisms as diverse as insects and mammals. The fruit fly Drosophila melanogaster express three closely related NF-kappaB-like transcription factors: Dorsal, Dif, and Relish. To study their roles in vivo, we used micr...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2007.04.001
更新日期：2008-01-01 00:00:00
abstract：:Monoclonal antibodies (mAbs) specific for a putative antigen receptor (function associated molecule, FAM) on fish NCC were tested for their ability to initiate signal transduction responses. Anti-FAM hybridoma target cells were significantly lysed by NCC when tested by redirected lysis (RL). Redirected lysis was enhan...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(92)90040-j
更新日期：1992-09-01 00:00:00
abstract：:Kazal-type serine proteinase inhibitors (KPIs) function in physiological and immunological processes requiring proteinase action. In the present study, the first Cherax quadricarinatus KPI gene (designated CqKPI) was identified and characterized. The open reading frame of CqKPI contains 405 nucleotides and encodes a p...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2020.103871
更新日期：2021-01-01 00:00:00
abstract：:Macroautophagy (hereinafter called autophagy) is a highly regulated process used by eukaryotic cells to digest portions of the cytoplasm that remodels and recycles nutrients and disposes of unwanted cytoplasmic constituents. Currently 36 autophagy-related genes (ATG) and their homologs have been characterized in yeast...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2015.02.017
更新日期：2015-07-01 00:00:00
abstract：:The respiratory glycoprotein, hemocyanin (HMC) has multiple immune-related functions, including antiviral activity. In this study, in silico methods were used to predict seven miRNAs targeting Litopenaeus vannamei HMC (LvHMC), out of which miR-589-5p was selected for further investigation because of its role in immune...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2020.103642
更新日期：2020-06-01 00:00:00
abstract：:Interleukin-1β (IL-1β), an inflammatory cytokine of the IL-1 family, is primarily produced as a precursor protein by monocytes and macrophages, then matures and becomes activated through proteolytic catalysis. Although the biological characteristics of avian IL-1β are well known, little information is available about ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2016.12.004
更新日期：2017-04-01 00:00:00
abstract：:Recent reports demonstrate that octopamine plays an important immunological role in crowded larvae of the Oriental Armyworm, Mythmina separata. We identified an octopamine receptor, the β-adrenergic-like gene (designated MsOctβ2R), with a 1191 bp open reading frame that encodes 396 amino acids and contains seven conse...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2020.103802
更新日期：2020-12-01 00:00:00
abstract：:We have purified five types of lectins, named tachylectins, from circulating hemocytes and hemolymph plasma of the Japanese horseshoe crab, Tachypleus tridentatus. Tachylectin-1 interacts with Gram-negative bacteria probably through 2-keto-3-deoxyoctonate, one of the constituents of lipopolysaccharides (LPS). Tachylec...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：10.1016/s0145-305x(99)00019-1
更新日期：1999-06-01 00:00:00
abstract：:Infectious hematopoietic necrosis virus (IHNV) is a common pathogen that causes severe disease in the salmonid aquaculture industry. Recent work demonstrated that autophagy plays an important role in pathogen invasion by activating innate and adaptive immunity. This study investigated the relationship between IHNV and...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2017.07.025
更新日期：2017-12-01 00:00:00
abstract：:Insects are able to effectively recognize parasitoid eggs or larvae and to eliminate them by formation of a hemocytic capsule. Although the cellular process is now well documented, the genetic aspects of recognition of foreignness and the encapsulation process are still poorly understood. Experiments using the isofema...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(85)90112-0
更新日期：1985-04-01 00:00:00
abstract：:Viral responsive protein 15 (PmVRP15) has been identified as a highly up-regulated gene in the hemocyte of white spot syndrome virus (WSSV)-infected shrimp Penaeus monodon. However, the function of PmVRP15 in host-viral interaction was still unclear. To elucidate PmVRP15 function, the interacting partner of PmVRP15 fr...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2016.01.020
更新日期：2016-06-01 00:00:00
abstract：:Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in mammals and play an important role in innate immunity. In the present study, a TLR5M gene was cloned from turbot, Scophthalmus maximus, and its immune responsive expression was subsequently studied in vivo. The Scophthalmus maximu...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2018.03.020
更新日期：2018-08-01 00:00:00
abstract：:Neonates of all species, including foals, are highly susceptible to infection, and neutrophils play a crucial role in innate immunity to infection. Evidence exists that neutrophils of neonatal foals are functionally deficient during the first weeks of life, including expression of cytokine genes such as IFNG. We hypot...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2018.06.012
更新日期：2018-10-01 00:00:00
abstract：:We previously reported the isolation of a lipopolysaccharide (LPS)-specific immulectin-2 from the tobacco hornworm, Manduca sexta [J. Biol. Chem. 275 (2000) 37373]. Immulectin-2 is a C-type lectin that is present at a constitutively low level in hemolymph of naive larvae, and its synthesis is induced after injection o...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/s0145-305x(02)00099-x
更新日期：2003-03-01 00:00:00
abstract：:Trypanosoma carassii is an extracellular parasite of economically important fish species that has evolved several strategies to circumvent host immune responses. Proteomic analysis of the excreted/secreted (ES) and surface molecules of the parasite has revealed a number of proteins that may be involved in host-parasit...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2009.11.005
更新日期：2010-04-01 00:00:00
abstract：:There is increasing concern about the possible effect of pharmaceutical compounds may have on the fish immune system. Bath exposition of 17α-ethynylestradiol (EE2), a synthetic estrogen used in oral contraceptives, altered the immune response of the gilthead seabream (Sparus aurata L.), a marine hermaphrodite teleost....
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2015.06.014
更新日期：2015-12-01 00:00:00
abstract：:Three molecular forms of immunoglobulins: pentamer, dimer and monomer, were isolated from serum of the frill shark, Chlamydoselachus anguineus, the most primitive extant shark. A pentamer having an apparent mass of 900 kDa, consisting of 68 kDa heavy (H) chains and 22-24 kDa light (L) chains, was considered to be IgM ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(92)90004-v
更新日期：1992-07-01 00:00:00
abstract：:Pro-inflammatory cytokines are produced as part of innate immunity. Increased resistance to extraintestinal Salmonella enteritidis (SE) has been associated with an increase in heterophil pro-inflammatory cytokine gene expression. Invasion of chicken epithelial cells by SE induces an 8- to 10-fold increase in interleuk...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2004.08.002
更新日期：2005-01-01 00:00:00
abstract：:Surfactant Protein A (SP-A) is a collagenous C-type lectin (collectin) that plays an important role in the early stage of the host immune response. In chicken, SP-A (cSP-A) is expressed as a 26 kDa glycosylated protein in the lung. Using immunohistochemistry, cSP-A protein was detected mainly in the lung lining fluid ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2016.03.010
更新日期：2016-08-01 00:00:00
abstract：:Mx proteins are antiviral GTPases, which are induced by type I IFN and virus infection. Analysis of the Atlantic salmon genome revealed the presence of 9 Mx genes localized to three chromosomes. A cluster of three Mx genes (SsaMx1 - SsaMx3), which includes previously cloned Mx genes, is present on chromosome (Chr) 12....
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2018.09.004
更新日期：2019-01-01 00:00:00
abstract：:To improve the efficacy of WSSV protection, multimeric (tetrameric) recombinant VP28 (4XrVP28) was produced and tested in comparison with those of monomeric VP28 (1XrVP28). In vitro binding of either 1XrVP28 or 4XrVP28 to shrimp hemocyte surface was evident as early as 10 min after protein inoculation. Similar results...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2017.05.016
更新日期：2017-11-01 00:00:00
abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) is an emerging animal virus that has caused high economic losses for the swine industry worldwide. Previous in vitro and in vivo studies demonstrated that PRRSV infection induces significant production of interleukin 10 (IL-10), a pleiotropic cytokine with im...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2012.10.001
更新日期：2013-03-01 00:00:00
abstract：:Interferon (IFN) regulatory factor 3 (IRF3) is a major regulator contributing to the host away from viral infection. Here, an IRF3 gene from sea perch (LjIRF3) was identified and its role in regulating early apoptosis signaling and IFN response was investigated during red spotted grouper nervous necrosis virus (RGNNV)...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2018.07.014
更新日期：2018-11-01 00:00:00
abstract：:Spleen cells from rats 2 to 132 days old were cultured with 1-125 micrograms/ml Concanavalin A (Con A). At high doses of Con A, the high spontaneous thymidine uptake of spleen cells from rats 15 to 21 days old was suppressed, whereas spleen cells from younger rats showed no suppression of spontaneous mitogenesis at eq...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(84)90073-9
更新日期：1984-10-01 00:00:00
abstract：:Transforming growth factor-β activated kinase 1 (TAK1) is a crucial signal transducer in multiple signaling pathways. TAK1 binds TAB1, TAB2, and TAB3, which act as activators and adaptors that specifically regulate the activation of TAK1. To date, the role of TABs is largely unknown in fish. In the present study, a TA...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2018.09.014
更新日期：2019-01-01 00:00:00
