Abstract：
:The rainbow trout caspase 6 gene has been cloned and sequenced. The open reading frame consisted of 906bp, which translated into a protein of 302 amino acids, containing the caspase active site pentapeptide (QACRG) and the caspase family signature (HADADCFVCVFLSHG). Amino acids involved in catalysis and those known to form the P1 carbohydrate binding pocket were conserved. Phylogenetic tree analysis showed a tight grouping with other known caspase 6 genes. Conserved aspartic acid residues at positions 33, 191 and 202 suggested that this molecule is produced as a proenzyme that is subsequently cleaved to release active subunits, with the region between Asp-191 and Ala-203 acting as a linker that is cleaved out. RT-PCR analysis revealed that the trout caspase 6 gene was expressed in brain, blood, gill, liver, head kidney and spleen. Addition of LPS or cortisol to head kidney leucocyte cultures had no effect upon caspase 6 expression. However, addition of LPS after preincubation with cortisol increased expression relative to control cultures. Incubation with RU486 abrogated this effect, confirming it was mediated via glucocorticoid receptors. Lastly, a confinement stress in vivo increased caspase 6 expression. The data are discussed with respect to the immunoregulatory role of apoptosis in fish immune responses.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Laing KJ,Holland J,Bonilla S,Cunningham C,Secombes CJdoi
10.1016/s0145-305x(00)00061-6keywords:
subject
Has Abstractpub_date
2001-05-01 00:00:00pages
303-12issue
4eissn
0145-305Xissn
1879-0089pii
S0145-305X(00)00061-6journal_volume
25pub_type
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：2012-04-01 00:00:00
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
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更新日期：2000-03-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：2012-01-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：10.1016/j.dci.2004.07.007
更新日期：2005-01-01 00:00:00
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2004.07.001
更新日期：2005-01-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：2019-06-01 00:00:00
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journal_title：Developmental and comparative immunology
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更新日期：2006-01-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：2012-01-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：1985-07-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
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更新日期：2019-07-01 00:00:00
abstract：:Bcl-2-associated X (Bax) belongs to the Bcl-2 protein family and its pro-apoptotic function has been confirmed in many vertebrate species. However, the functional role of Bax in apoptosis in invertebrates is limited. Here, a Bax homologue (AjBax) in Apostichopus japonicas was cloned and characterized, and its pro-apop...
journal_title：Developmental and comparative immunology
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更新日期：2020-01-01 00:00:00
