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Chicken cathelicidins as potent intrinsically disordered biocides with antimicrobial activity against infectious pathogens.

Abstract：

:This study was performed to identify the expression patterns of the cathelicidin genes in a local chicken breed and to evaluate the antimicrobial activities of the cathelicidin peptides against pathogenic bacteria. This analysis revealed that the coding regions of CATH-1, -2, and -3 genes contain 447 bp, 465 bp, and 456 bp, respectively, and encode proteins of 148, 154, 151 amino acids, respectively. The complete amino acid sequences of the cathelicidin peptides are similar to those found in Meleagris gallopavo, Phasianus colchicus, and Coturnix coturnix, and show high sequence identity to their Columba livia and Anas platyrhynchos counterparts. In contrast, these avian peptides shared a very low sequence identity with the mammalian cathelicidins. The analysis further revealed that the cathelicidin genes are expressed in various organ and tissues. We also show that the CATH peptides 1, 2, 3 and their amide-modified structures possess potent antimicrobial activities against both Gram-positive and Gram-negative pathogens, with these bacteria being affected to different extents. The antimicrobial activities of the peptides are slightly lower than those of their amide analogs. Computational analysis revealed that pre-pro-cathelicidins are hybrid proteins that contain ordered domains and functional intrinsically disordered regions. Furthermore, high structural and sequence variability of mature cathelicidins is a strong indication of their rather disordered nature. It is likely that intrinsic disorder is needed for the multifarious functionality of these antimicrobial peptides. Our analyses indicated that cathelicidin peptides require further study to better understand their full potentials in the treatment of diseases in both humans and animals. The data obtained for synthetic avian peptides will help elucidating of their potential applications in the pharmaceutical industry.

journal_name

Dev Comp Immunol

journal_title

Developmental and comparative immunology

authors

Yacoub HA,Elazzazy AM,Mahmoud MM,Baeshen MN,Al-Maghrabi OA,Alkarim S,Ahmed ES,Almehdar HA,Uversky VN

doi

10.1016/j.dci.2016.06.012

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

8-24

eissn

0145-305X

issn

1879-0089

pii

S0145-305X(16)30194-X

journal_volume

pub_type

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Chicken cathelicidins as potent intrinsically disordered biocides with antimicrobial activity against infectious pathogens.