Abstract：
:The in vitro binding serum complement (C) components to Schistosoma mansoni worms was studied. By exposing frozen sections of adult male and female parasites to normal human serum, binding of both classical and alternative pathway C components was observed. By immunofluorescence (IFL) microscopy selective binding of Clq, C4 and C3 to the schistosomal tegument, internal structures, the intestinal tract and eggs was seen. The C4 and C3 binding was completely abolished in the presence of 10 mM EDTA. EGTA also completely inhibited binding of C4 and most of the C3 binding. These results suggest that C binding to S. mansoni adult worms occurs mostly by the classical activation pathway. However, the partial Ca2+ independence of C3 binding and the demonstrated binding of the regulatory protein beta 1H suggests that worms are capable also of C3 binding by the alternative pathway. No C binding occurred to intact worms. Although some in vivo bound immunoglobulin appeared to be present occasionally at the parasite surface and in the gut, this material did not account for the demonstrated extensive C deposition upon incubation of frozen sections with normal human serum in vitro. Antibody independence of the observed classical pathway C binding was further indicated by binding of isolated Clq to the same structures capable of binding C components from serum.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Linder E,Huldt Gdoi
10.1111/j.1365-3024.1983.tb00736.xsubject
Has Abstractpub_date
1983-03-01 00:00:00pages
183-94issue
2eissn
0141-9838issn
1365-3024journal_volume
5pub_type
杂志文章abstract：:Malaria parasites have a complex, multistage life cycle and there is a widely held view that each stage displays a distinct set of antigens presented to the immune system. Yet, molecular analysis of malaria parasites suggests that many putative antigenic targets are shared amongst the different stages. The specificiti...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/pim.12434
更新日期：2017-07-01 00:00:00
abstract：:Cells collected from C57B1/6 mice infected with monomorphic and pleomorphic clones of Trypanosoma brucei parasites (ILTat 1.4 and GUTat 3.1) were analysed for the incorporation of 125I-Iododeoxyuridine into DNA of total splenic lymphocytes and of B and T lymphocytes isolated on a fluorescence activated cell sorter. Th...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1986.tb00860.x
更新日期：1986-09-01 00:00:00
abstract：:Attempts to produce monoclonal antibodies specific for a parasite-encoded or induced antigen on the surface of Theileria parva lawrencei-transformed bovine lymphocytes resulted in the identification of the monoclonal antibody J7. This antibody recognizes a membrane glycoprotein on some T.p. lawrencei-infected bovine l...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1986.tb00841.x
更新日期：1986-03-01 00:00:00
abstract：:Two monoclonal antibodies against the surface of S. mansoni schistosomula were found to confer significant passive protection to mice (M7B3A, range 28-70%; M22H12C, range 14-58%). No additive effect was observed when both were transferred together. Neither McAb bound to the cercarial surface but both bound to the surf...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1986.tb00836.x
更新日期：1986-01-01 00:00:00
abstract：:In vitro released products of the adult stage of the bovine lungworm. Dictyocaulus viviparus, were characterized according to their SDS-PAGE profile, glycosylation pattern, in vitro synthesis and antigenicity in the context of infection and vaccination with irradiated larvae. Biosynthetic labelling experiments with 35...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1993.tb00596.x
更新日期：1993-03-01 00:00:00
abstract：:Groups of five dogs were vaccinated against Babesia canis using soluble parasite (SPA) antigens from in vitro cultures. Although vaccination did not significantly alter peripheral parasitaemia upon challenge, protected animals had lower levels of SPA in the plasma after a challenge infection. The severity of anaemia c...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3024.1996.d01-1.x
更新日期：1996-01-01 00:00:00
abstract：:A 120 kDa antigen containing SDS-PAGE fraction BmA-2 isolated from Brugia malayi adult parasite was highly reactive with normal sera from filarial endemic area. BmA-2 was analysed for its prophylactic potential in in vitro and in vivo. Sera collected from BmA-2 immunized jirds induced a significant level (80 to 90%) o...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1995.tb00893.x
更新日期：1995-06-01 00:00:00
abstract：:Parasitic nematodes of the small-ruminant gastrointestinal tract pose a problem worldwide. The impact of these pathogens is worsened by the emergence of anthelmintic resistance to all three available classes of drugs. In addition to causing considerable economic loss, these parasites are detrimental to the health and ...
journal_title：Parasite immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-3024.2009.01110.x
更新日期：2009-07-01 00:00:00
abstract：:Haemonchus contortus is an economically important parasite that survives the host defense system by modulating the immune response. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is secreted by the parasite and the host responds by producing anti-enzyme antibodies. The enzyme inhibits complement cascade, an arm of t...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/pim.12611
更新日期：2019-02-01 00:00:00
abstract：:Analysis by one-dimensional (1-D) SDS-PAGE/Western blotting of whole mite extract (larval and adult stages) with sheep sera taken 0-84 days after infection with the sheep scab mite, Psoroptes ovis revealed a progressive IgE antibody response, with a dominant high molecular weight allergen (MW 180 kDa) detected early d...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.0141-9838.2004.00717.x
更新日期：2004-08-01 00:00:00
abstract：:Plasma from chronic or advanced hepatosplenic schistosomiasis (mansoni) patients (PCS) suppressed mitogen-induced responses of normal human peripheral blood mononuclear cells. 1 x 10(5) Ficoll-Hypaque isolated lymphocytes were cultured in 100 microliter RPMI 1640 media with 100 microliter plasma preparation and stimul...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1982.tb00439.x
更新日期：1982-07-01 00:00:00
abstract：:In this study we have investigated the ability of the stage-specific monoclonal antibody (MoAb) 1G7 to neutralize the infectivity of Trypanosoma cruzi metacyclic trypomastigotes (Tulahuen strain). Mice inoculated with metacyclics pre-incubated with 1G7 or the corresponding Fab fragments were found to develop greatly r...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1988.tb00256.x
更新日期：1988-11-01 00:00:00
abstract：:Cryptocaryon irritans is one of the most important ectoparasites of marine fish. To identify the potential role of immune-related genes in antiparasitic immune responses in fish, we monitored the expression change of IL-8, COX-2, C-type lectin and transferrin in local and systemic immune organs of orange-spotted group...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.2011.01337.x
更新日期：2011-12-01 00:00:00
abstract：:The role of autoimmunity in the pathogenesis and progression of heart lesions in the chronic phase of Chagas' disease is controversial. In the absence of parasites in situ, the T cell infiltrate seen in heart lesions may be the primary determinant of tissue damage ultimately leading to heart failure and death. We used...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1994.tb00337.x
更新日期：1994-04-01 00:00:00
abstract：:In the present study, the cytokines interleukin (IL)-12 and IL-18 were evaluated for their capacity to modulate and to re-direct in vitro parasite antigen-specific cellular responsiveness in patients exposed to Onchocerca volvulus and Entamoeba histolytica infection. We found that IL-18 was highly capable of reducing ...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3024.2003.00638.x
更新日期：2003-06-01 00:00:00
abstract：AIMS:Our aim was to investigate the skin-homing T-cell immune responses triggered in patients with Demodex infestation and/or rosacea. METHODS:Collected whole blood samples were divided into four groups: control subjects; nonrosacea patients with Demodex infestation (Demodex group); papulopustular rosacea (PPR) patien...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/pim.12658
更新日期：2019-08-01 00:00:00
abstract：:Athymic nude mice of the outbred Zur:ICR-nu and inbred BALB/c strain and scid mice were infected with a cloned human isolate of Giardia lamblia (GS/M-83-H7). Changes in the expression of the major surface epitope of the intestinal trophozoites (characterized by the binding capacity of monoclonal antibody MoAbG10/4) as...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1991.tb00560.x
更新日期：1991-11-01 00:00:00
abstract：:Infective larvae of Toxocara canis are well suited for studies of nematode antigen expression in vitro. Larvae were labelled with 3H-glucosamine, an approach permitting dual analysis of antigen quantity and composition. Their excretory/secretory (E/S) glycoproteins were efficiently labelled and antigen identity confir...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1990.tb00946.x
更新日期：1990-03-01 00:00:00
abstract：:Type I hypersensitivity reactions in the intestinal tract of sensitized animals may contribute to resistance to reinfection with Fasciola hepatica. Colonic mucosae isolated from previously infected rats were voltage clamped in Ussing chambers. Antigen was prepared as a crude homogenate from adult liver fluke. Assay of...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1993.tb00630.x
更新日期：1993-08-01 00:00:00
abstract：:As an orally acquired pathogen, the immune response to Toxoplasma gondii unfolds in the small intestinal mucosa. There, an array of regulatory and effector immune cells are elicited to combat the parasite through secretion of inflammatory mediators, normally resulting in host protection and pathogen control. Recent st...
journal_title：Parasite immunology
pub_type： 杂志文章,评审
doi：10.1111/pim.12164
更新日期：2015-03-01 00:00:00
abstract：:The Bacillus thuringiensis Cry1Ac protoxin (pCry1Ac) is a promising mucosal immunogen and adjuvant that induces protective immunity against Naegleria fowleri and malaria infection models. We determined whether pCry1Ac acted as a protective adjuvant against infection with Taenia crassiceps. BALB/C mice were thrice i.p....
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/pim.12103
更新日期：2014-06-01 00:00:00
abstract：:Immunity to Schistosoma mansoni in the mouse was induced by vaccination with adult worm surface membrane (mb-S). Of several adjuvants tested, including Freund's, BCG and alum, 50 micrograms of saponin per mouse given subcutaneously with the antigen was the easiest to administer, and gave consistent protection, approac...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1989.tb00669.x
更新日期：1989-07-01 00:00:00
abstract：:HIV and T. gondii infection markers were measured among 383 Intravenous Drug Users (IDU). And cytokine concentrations (IL-4, IL-6, IL-10, IL-12, IFN-gamma and TNF-alpha) were determined. The results showed IDU with HIV infection or HIV/T. gondii co-infection could disturb Th regulatory mechanism. ...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.2009.01174.x
更新日期：2010-02-01 00:00:00
abstract：:Data from experimental and human cryptosporidiosis have established a major role of specific immunity in the control of Cryptosporidium parvum infection. In this work, alterations in spleen and Peyer's patch (Pp) lymphocytes were investigated in the course of a spontaneously resolutive gut cryptosporidiosis in four-da...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.0141-9838.2004.00676.x
更新日期：2004-01-01 00:00:00
abstract：:We describe an ELISA for trophozoite-specific IgA in the intestinal secretions of mice infected with Giardia muris. Using this method, trophozoite-specific IgA was demonstrated in intestinal secretions of Giardia-infected immunocompetent BALB/c mice. Such antibody was undetectable in the intestinal secretions of Giard...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1988.tb00257.x
更新日期：1988-11-01 00:00:00
abstract：:Sheep scab is caused by the noninvasive mite, Psoroptes ovis, which initiates a profound pro-inflammatory skin response leading to lesion development. To investigate these early events between the skin and the parasite, primary ovine epidermal keratinocyte cultures were generated and challenged with mite derived antig...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.2009.01103.x
更新日期：2009-06-01 00:00:00
abstract：:The K1 peptide is a CD8(+)T cell HLA-A*0201-restricted epitope derived from the Trypanosoma cruzi KMP-11 protein. We have previously shown that this peptide induces IFN-gamma secretion by CD8(+)T cells. The aim of this study was to characterize the frequency of K1-specific CD8(+)T cells in chagasic patients. Nineteen ...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.2010.01206.x
更新日期：2010-07-01 00:00:00
abstract：:Cryptosporidiosis is a zoonotic protozoan disease that affects the gastrointestinal tract of animals and humans. Diarrhoea as the most important indication of the infection leads to high economic losses in livestock industries and is a life threatening infection in immunocompromised individuals. In the absence of the ...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/pim.12317
更新日期：2016-05-01 00:00:00
abstract：:Although the precise host-defence mechanisms are not completely understood, T-cell-mediated immune responses are believed to play a pivotal role in controlling parasite infection. In this study, the potential HLA*A2 restricted peptides were predicted and the ability of peptides to bind HLA-A*02 was confirmed by a MHC ...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/pim.12451
更新日期：2017-09-01 00:00:00
abstract：:Naegleria fowleri, a free-living amoeba, is the causative pathogen of primary amoebic meningoencephalitis in humans and experimental mice. N. fowleri is capable of destroying tissues and host cells through lytic necrosis. However, the mechanism by which N. fowleri induces host cell death is unknown. Electron microscop...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.2011.01297.x
更新日期：2011-07-01 00:00:00
