Abstract:
:Athymic nude mice of the outbred Zur:ICR-nu and inbred BALB/c strain and scid mice were infected with a cloned human isolate of Giardia lamblia (GS/M-83-H7). Changes in the expression of the major surface epitope of the intestinal trophozoites (characterized by the binding capacity of monoclonal antibody MoAbG10/4) as well as cellular and humoral immune parameters of the hosts were followed during the course of infection. Self-cure was observed in heterozygous (nu/+) BALB/c mice by day 22 post-infection (p.i.) and in heterozygous (nu/+) Zur:ICR-nu strain by day 65 p.i. Homozygous (nu/nu) mice of both strains remained chronically infected until end of the experiments (day 45 p.i. for BALB/c mice and day 122 p.i. for Zur:ICR-nu mice, respectively). Only heterozygous (nu/+) mice were able to mount a gut-associated (Peyer's patch) lymphoproliferative response to G. lamblia antigen. Therefore, T-cell dependent mechanisms were necessary for a self-cure. Antigenic variation occurred in all nu/+ and nu/nu animals of both strains. Trophozoites expressing the major surface epitope (assessed by direct immunofluorescence with FITC-labelled MoAb G10/4) decreased to zero by day 22 p.i. In contrast, the proportion of trophozoites expressing the major surface epitope in infected scid mice remained at the initial level (greater than 99%) until termination of the experiment (day 25 p.i.); therefore, antigenic variation did not occur. All nu/nu and nu/+ mice but not scid mice demonstrated a humoral immune response to G. lamblia antigen. These experiments suggest functional B-cell dependent mechanisms are most likely responsible for the surface antigen switch. Transfer of infection occurred naturally from experimentally infected scid-mice to their mother, proving the initial antigenic surface variant remains unchanged after encystment and subsequent excystment followed by infection in a new host.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Gottstein B,Nash TEdoi
10.1111/j.1365-3024.1991.tb00560.xsubject
Has Abstractpub_date
1991-11-01 00:00:00pages
649-59issue
6eissn
0141-9838issn
1365-3024journal_volume
13pub_type
杂志文章abstract::The natural site of infection for T. gondii is the mucosal surface of the intestine, so the protective immunity obtained after natural infection with T. gondii points to the importance of developing a vaccine that stimulates mucosal defences. In this study, an aroA- and aroD- attenuated strain of Salmonella typhimuriu...
journal_title:Parasite immunology
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journal_title:Parasite immunology
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1995.tb00893.x
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journal_title:Parasite immunology
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1996.d01-62.x
更新日期:1996-03-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1982-07-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1994-08-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1993-11-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2007.00938.x
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:2009-08-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1988.tb00256.x
更新日期:1988-11-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1995-03-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章,随机对照试验
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更新日期:2017-06-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:2004-06-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1995-02-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:2019-09-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1989-07-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:2002-11-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1983-09-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1993.tb00641.x
更新日期:1993-09-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-3024.2006.00824.x
更新日期:2006-07-01 00:00:00
abstract::Advances in hookworm immunoepidemiology are reviewed. Recent studies demonstrate a mixed Th1/Th2 response in human hookworm infection, with immunosuppression of specific and nonspecific IFN-gamma responses. There is increasing evidence for protective immunity in human hookworm infection, including anti-larval IL-5- an...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.0141-9838.2004.00727.x
更新日期:2004-11-01 00:00:00