Abstract:
:Use of the pig as an animal model in schistosomiasis research is increasing, but knowledge of the porcine immune response to schistosome infection is still very limited. We investigated the immunohistology of different maturation stages of the Schistosoma japonicum egg granuloma in pigs. Liver sections from pigs experimentally infected with S.japonicum for 9, 12 or 21 weeks were examined by immunohistochemistry using a three-step streptavidin-biotin-complex/immunoperoxidase method or a two-step alkaline phosphatase-mediated system. All granulomas showed marked expression of major histocompatibility complex (MHC) class II in epithelioid macrophages and were dominated by T lymphocytes, comprising both CD4+ and CD8+ phenotypes, with consistently higher proportions noted for CD8+ cells. B lymphocytes, as identified by expression of CD21, were confined to lymphoid nodular structures primarily associated with mature granulomas. Early and mature granulomas contained numerous immunoglobulin (Ig)G+ plasma cells. Significant differences in immunohistology related to duration of infection were not observed. The results indicate that all stages of the hepatic S.japonicum egg granuloma in the pig manifests MHC class II-dependent CD4+ T cell activity concomitant with infiltration of CD8+ T cells. B cell activity preceding the effector cell stage appears to occur in granuloma-associated lymphoid nodules, whereas antibody, mainly IgG, is produced within the granuloma.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Hurst MH,Willingham AL,Lindberg Rdoi
10.1046/j.1365-3024.2002.00448.xkeywords:
subject
Has Abstractpub_date
2002-03-01 00:00:00pages
151-9issue
3eissn
0141-9838issn
1365-3024pii
448journal_volume
24pub_type
杂志文章abstract::We have studied the properties of 16 newly derived monoclonal antibodies (MoAbs) against Pfs230, a gamete surface protein of Plasmodium falciparum and a target of transmission-blocking antibodies. All 16 MoAbs recognized Pfs230 by immunoprecipitation from non-ionic detergent extracts of the protein radio-labelled with...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1994.tb00305.x
更新日期:1994-10-01 00:00:00
abstract::We assayed the vaccine potentialities of a recombinant S. bovis-derived glutathione S-transferase (rSb28GST), member of a molecular family already shown to have protective capacities in the S. mansoni and S. japonicum models. Injection of the rSb28GST in Freund's Complete Adjuvant resulted in good specific IgG respons...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1994.tb00367.x
更新日期:1994-08-01 00:00:00
abstract::The effect of infection and subsequent challenge with Eimeria maxima on the populations of lymphocytes in the small intestine of Light Sussex chickens was assessed by immunohistochemistry. T cells were characterized for CD3, CD4, CD8, TCR1 (gamma delta heterodimer) or TCR2 (alpha beta 1 heterodimer) markers, and B cel...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1995.tb00883.x
更新日期:1995-10-01 00:00:00
abstract:BACKGROUND:More than 11 million people were estimated to have been infected by Schistosoma japonicum in China before the 1950s. However, few studies have been conducted to evaluate the longitudinal effects of previous schistosome infection (PSI). OBJECTIVE:We aimed to evaluate the association of PSI with fatty liver a...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12822
更新日期:2021-01-17 00:00:00
abstract::Although the precise host-defence mechanisms are not completely understood, T-cell-mediated immune responses are believed to play a pivotal role in controlling parasite infection. In this study, the potential HLA*A2 restricted peptides were predicted and the ability of peptides to bind HLA-A*02 was confirmed by a MHC ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12451
更新日期:2017-09-01 00:00:00
abstract::A recent working group convened by the World Health Organization recommended that time to first or only episode of clinical malaria should be used to evaluate vaccine efficacy in phase III trials. However, calculating vaccine efficacy based on this endpoint misses important aspects of malaria disease and transmission....
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-3024.2009.01144.x
更新日期:2009-09-01 00:00:00
abstract::Isotype/subclass-specific antibody responses to adult Onchocerca volvulus extract (OvAg) were assessed by both ELISA and immunoblotting for a group of putatively immune individuals (PIs, n = 29) from a hyperendemic area in Ecuador and for a group of infected individuals (INFs, n = 470) from the same regions. As a grou...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1995.tb00904.x
更新日期:1995-07-01 00:00:00
abstract::Neospora caninum is a cyst-forming coccidian parasite which in cattle can cause abortion and birth of feeble calves. For the serological diagnosis of neosporosis, antibodies directed to the parasite can be demonstrated by ELISA, utilizing N. caninum proteins incorporated into iscoms (immunostimulating complexes) as an...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1998.00127.x
更新日期:1998-02-01 00:00:00
abstract::The serodiagnosis of human toxocariasis is difficult. Specific IgGs detected routinely with ELISA based on Toxocara excretory-secretory (TES) antigens often persist for years at an elevated level, which does not allow either the differentiation between an active and persistent infection or monitoring of the effect of ...
journal_title:Parasite immunology
pub_type: 临床试验,杂志文章
doi:10.1111/pim.12400
更新日期:2017-01-01 00:00:00
abstract::In vitro released products of the adult stage of the bovine lungworm. Dictyocaulus viviparus, were characterized according to their SDS-PAGE profile, glycosylation pattern, in vitro synthesis and antigenicity in the context of infection and vaccination with irradiated larvae. Biosynthetic labelling experiments with 35...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1993.tb00596.x
更新日期:1993-03-01 00:00:00
abstract::Analysis by one-dimensional (1-D) SDS-PAGE/Western blotting of whole mite extract (larval and adult stages) with sheep sera taken 0-84 days after infection with the sheep scab mite, Psoroptes ovis revealed a progressive IgE antibody response, with a dominant high molecular weight allergen (MW 180 kDa) detected early d...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.0141-9838.2004.00717.x
更新日期:2004-08-01 00:00:00
abstract::Infective forms of Trypanosoma cruzi, the parasite that causes Chagas' disease, express on their surface an enzyme denominated trans-sialidase (TS). The present study was designed to evaluate the naturally acquired immune responses to a bacterial recombinant protein representing the catalytic domain of TS in chronical...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2000.00260.x
更新日期:2000-01-01 00:00:00
abstract::Type I hypersensitivity reactions in the intestinal tract of sensitized animals may contribute to resistance to reinfection with Fasciola hepatica. Colonic mucosae isolated from previously infected rats were voltage clamped in Ussing chambers. Antigen was prepared as a crude homogenate from adult liver fluke. Assay of...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1993.tb00630.x
更新日期:1993-08-01 00:00:00
abstract::The anti-leishmanial effect of the 'carbohydrate-fraction', isolated from an edible mushroom Astraeus hygrometricus, was evaluated against Leishmania donovani infection both in vitro and in vivo. Ahf-Car induced the expression of inducible nitric oxide synthase 2 (iNOS2) and pro-inflammatory cytokines like TNF-α and I...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12806
更新日期:2020-10-31 00:00:00
abstract::Serum antibody responses of mice exposed to Philippine isolates of Schistosoma japonicum have been analysed by immunoprecipitation of exogenously radiolabelled antigens extracted from adult worms. Attention was focused on labelled protein antigens differentially recognized by sera of mice that differ genetically in th...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1985.tb00067.x
更新日期:1985-03-01 00:00:00
abstract::HIV and T. gondii infection markers were measured among 383 Intravenous Drug Users (IDU). And cytokine concentrations (IL-4, IL-6, IL-10, IL-12, IFN-gamma and TNF-alpha) were determined. The results showed IDU with HIV infection or HIV/T. gondii co-infection could disturb Th regulatory mechanism. ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2009.01174.x
更新日期:2010-02-01 00:00:00
abstract::Toxoplasma gondii is a highly successful parasite that can manipulate host immune responses to optimize its persistence and spread. As a result, a highly complex relationship exists between T. gondii and the immune system of the host. Advances in imaging techniques, and in particular, the application of two-photon mic...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12161
更新日期:2015-03-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2005.00760.x
更新日期:2005-05-01 00:00:00
abstract::A 17 kD protein of Necator americanus was isolated by SDS-PAGE and used to raise monospecific antisera in rabbits. ELISA and Western blotting against a range of parasite extracts demonstrated the species specificity of this protein. It is expressed at all stages of the life-cycle, appears to be accumulated through the...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1990.tb00953.x
更新日期:1990-05-01 00:00:00
abstract::In this study, supplementation with the probiotic Saccharomyces boulardii promoted a reduction in intensity of infection by Toxocara canis and modulates cytokines mRNA expression in experimentally infected mice. IL-12 gene transcription had 40-fold increase in S. boulardii supplemented uninfected mice and sevenfold in...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12314
更新日期:2016-05-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1990.tb00946.x
更新日期:1990-03-01 00:00:00
abstract::Leishmaniasis is a parasitic disease transmitted by the bite of Leishmania-infected sand flies. Here we show for the first time the ability of Maxadilan (Max), a vasodilatory peptide isolated from the sand fly Lutzomyia longipalpis, and salivary gland lysate (SGL) from Phlebotomus papatasi to decrease the secretion of...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2003.00623.x
更新日期:2003-03-01 00:00:00
abstract::The immunological basis of resistance to Brugia pahangi infection in rats was studied. Infections were investigated in athymic rnu/rnu rats and in rats treated with the immuno-suppressive agents cyclosporin A (CsA) or cyclophosphamide (Cy). The recovery of adult worms in normal rats was 1-2% in comparison to 12.2% rec...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1992.tb00012.x
更新日期:1992-07-01 00:00:00
abstract::Urine and stool specimens from 425 school children in Swaziland were examined for evidence of Schistosoma mansoni or Schistosoma haematobium infection. Concurrently, saliva collections were analysed for ABH secretory ability and blood samples were typed for ABO, Rh and Lewis groups. Among individuals infected with S. ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1979.tb00701.x
更新日期:1979-07-01 00:00:00
abstract::Particular immunopathological features and their effects on the vascular permeability of different ocular structures were analysed in two dogs naturally infected by Leishmania infantum. The existence of specific anti-Leishmania immunoglobulin G (IgG) in the aqueous humour was confirmed by the ELISA technique. There wa...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1996.d01-39.x
更新日期:1996-12-01 00:00:00
abstract::Lymphoid cells from genetically-susceptible BALB/c mice immunized against a glycoconjugate of the protozoan parasite, Leishmania major, promote chronic cutaneous disease in BALB/c nude mice. This cell population therefore differs from cells harvested from non-immunized BALB/c mice that are known to be potent mediators...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1986.tb01037.x
更新日期:1986-05-01 00:00:00
abstract::Toxoplasma gondii-specific murine CD4+ T cell clone 3Tx9 belongs to the Th1 subtype by virtue of secreting high levels of interleukin(IL)-2, interferon-gamma and tumor necrosis factor without producing IL4 and IL10. To characterize the clonal antigen, Toxoplasma lysate-was separated by SDS-PAGE and probed in T cell bl...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1997.d01-201.x
更新日期:1997-05-01 00:00:00
abstract::Trypanosoma congolense is one of the main species responsible for Animal African Trypanosomosis (AAT). As preventive vaccination strategies for AAT have been unsuccessful so far, investigating the mechanisms underlying vaccine failure has to be prioritized. In T. brucei and T. vivax infections, recent studies revealed...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12099
更新日期:2014-05-01 00:00:00
abstract::Migration of human peripheral blood granulocytes in response to microfilariae of O. volvulus was demonstrated using modified Boyden chambers. Granulocyte migration was significantly enhanced when microfilariae were preincubated with heat-inactivated immune serum (delta IS), then added to a fresh serum source (P less t...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1983.tb00738.x
更新日期:1983-03-01 00:00:00
abstract::Eleven strains of Trypanosoma cruzi, originating from a variety of vertebrate and invertebrate hosts in distinct geographical regions, were examined for the reactivity of metacyclic stages with the monoclonal antibody 1G7. Trypomastigotes of five strains were susceptible to complement-dependent 1G7-mediated lysis. Hig...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1988.tb00227.x
更新日期:1988-07-01 00:00:00