Abstract:
:The serodiagnosis of human toxocariasis is difficult. Specific IgGs detected routinely with ELISA based on Toxocara excretory-secretory (TES) antigens often persist for years at an elevated level, which does not allow either the differentiation between an active and persistent infection or monitoring of the effect of treatment. Additionally, false-positive results may occur in co-infections with other helminths due to cross-reactions. We evaluated the usefulness of an IgG avidity index (AI) and a Western blotting (WB) IgG in the diagnosis of patients suspected of Toxocara infection. We studied 138 subjects who were submitted to serological testing two or more times. Confirmation of an infection by WB was achieved in 73.2% of patients. A high AI was obtained in 89.1% of patients, and low AI and borderline AI were found in only 10.9%. Low and borderline values of AI remained at similar levels in subsequent studies over 2-3 years. The results showed the necessity of obligatory verification of all ELISA IgG positive and questionable results by WB. The index of IgG avidity may be helpful in excluding recent infection, but its usefulness in detecting an active phase of invasion requires further research.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Rudzińska M,Kowalewska B,Sikorska Kdoi
10.1111/pim.12400subject
Has Abstractpub_date
2017-01-01 00:00:00issue
1eissn
0141-9838issn
1365-3024journal_volume
39pub_type
临床试验,杂志文章abstract::The p190 protein (also called MSA1 or MSP1) of the asexual blood stage forms of Plasmodium falciparum, a human malaria vaccine candidate, shows polymorphism between different isolates. Mice were immunized with p190-3, a recombinant protein which contains mostly conserved sequences derived from the p190 protein of the ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1993.tb00592.x
更新日期:1993-03-01 00:00:00
abstract::Haemonchus contortus is an economically important parasite that survives the host defense system by modulating the immune response. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is secreted by the parasite and the host responds by producing anti-enzyme antibodies. The enzyme inhibits complement cascade, an arm of t...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12611
更新日期:2019-02-01 00:00:00
abstract:AIMS:Industrial growth has increased the exposure to endocrine disruptor compounds (EDCs) in all organisms. Bisphenol A (BPA), an EDC, has been demonstrated to be involved in the susceptibility to parasite infections. However, few studies have analysed this connection in more depth. The aim of this study was to determi...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12733
更新日期:2020-09-01 00:00:00
abstract::In this study, we investigated the characteristics of the infection and subsequent immunity induced by Strongyloides venezuelensis in Lewis rats. Animals were infected with 4000 L3 of S. venezuelensis and number of eggs per gram of faeces indicated an acute phase around day 8 and a recovery phase around day 32 after i...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2009.01160.x
更新日期:2010-01-01 00:00:00
abstract::Malaria remains one of the most serious health problems globally, but our understanding of the biology of the parasite and the pathogenesis of severe disease is still limited. Multiple cellular effector mechanisms that mediate parasite elimination from the liver have been described, but how effector cells use classica...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12039
更新日期:2013-09-01 00:00:00
abstract::Serum antibody responses of mice exposed to Philippine isolates of Schistosoma japonicum have been analysed by immunoprecipitation of exogenously radiolabelled antigens extracted from adult worms. Attention was focused on labelled protein antigens differentially recognized by sera of mice that differ genetically in th...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1985.tb00067.x
更新日期:1985-03-01 00:00:00
abstract::Groups of five dogs were vaccinated against Babesia canis using soluble parasite (SPA) antigens from in vitro cultures. Although vaccination did not significantly alter peripheral parasitaemia upon challenge, protected animals had lower levels of SPA in the plasma after a challenge infection. The severity of anaemia c...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1996.d01-1.x
更新日期:1996-01-01 00:00:00
abstract::Although the precise host-defence mechanisms are not completely understood, T-cell-mediated immune responses are believed to play a pivotal role in controlling parasite infection. In this study, the potential HLA*A2 restricted peptides were predicted and the ability of peptides to bind HLA-A*02 was confirmed by a MHC ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12451
更新日期:2017-09-01 00:00:00
abstract::The soluble portion of a large particle fraction which was derived from the muscle larva of T. spiralis was subjected to molecular sizing column chromatography using Sephacryl S-200. Five major peaks of 280 nm absorbing material were obtained. Analysis by immunoelectrophoresis revealed that each peak contained antigen...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1981.tb00405.x
更新日期:1981-10-01 00:00:00
abstract::Previous studies have shown that Leishmania elongation initiation factor (LeIF) antigen causes a partial immunity against leishmaniasis. The antigen develops type I immunity by overexpression of inflammatory cytokines such as interleukin-12 (IL-12), IFN-γ and TNF-α. Therefore, We evaluated immune responses induced by ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12310
更新日期:2016-04-01 00:00:00
abstract::The impact of intestinal helminth infection, i.e. Ascaris lumbricoides and Trichuris trichiura, on cellular responsiveness and cytokine production was investigated in young adults. Ascaris-specific cellular responsiveness was higher in parasite-free endemic controls than in patients infected with T. trichiura, or A. l...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2002.00600.x
更新日期:2002-11-01 00:00:00
abstract::We have assessed the role of tumour necrosis factor-alpha (TNF) during cutaneous leishmaniasis and demonstrated that significant levels of TNF were released by spleen cells from infected mice after in vitro restimulation with Leishmania major promastigotes. Spleen cells from both genetically resistant and genetically ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1990.tb00983.x
更新日期:1990-09-01 00:00:00
abstract::We have tested the hypothesis that activation of T cells by exposure to malaria antigens facilitates both de novo HIV infection and viral reactivation and replication. PBMC from malaria-naive HIV-uninfected European donors could be productively infected with HIV following in vitro stimulation with a lysate of Plasmodi...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.0141-9838.2004.00701.x
更新日期:2004-05-01 00:00:00
abstract::An indirect enzyme linked immunsorbent assay (ELISA) for detection of antibodies to Neospora caninum in serum from dogs is described. Extracted tachyzoite proteins incorporated into immunostimulating complexes (iscoms) were used as coating antigen. A mixture of a monoclonal antibody to dog immunoglobulin G and a horse...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1994.tb00320.x
更新日期:1994-12-01 00:00:00
abstract::The pathogenesis of Acanthamoeba keratitis (AK) is complicated. In our previous studies, TLR4 was found involved in the process of infection by Acanthamoeba in human corneal cells. The purpose of this study was to investigate the role of Toll-like receptor 4 (TLR4) signalling pathway in Wistar rats challenged with Aca...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2010.01247.x
更新日期:2011-01-01 00:00:00
abstract::The C57BL/6 mouse strain is resistant to Leishmania (L.) major infection and, unlike susceptible BALB/c, develops small self-healing cutaneous lesions. The specific antibody responses of C57BL/6 and BALB/c mice were previously characterized by the predominance of IgG2a ('resistant' isotype associated with Th1) and IgG...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12223
更新日期:2015-10-01 00:00:00
abstract::We describe an ELISA for trophozoite-specific IgA in the intestinal secretions of mice infected with Giardia muris. Using this method, trophozoite-specific IgA was demonstrated in intestinal secretions of Giardia-infected immunocompetent BALB/c mice. Such antibody was undetectable in the intestinal secretions of Giard...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1988.tb00257.x
更新日期:1988-11-01 00:00:00
abstract::Leishmania donovani, the causative parasite of Visceral Leishmaniasis (VL), deviously manipulates host monocytes/macrophages to ensure its survival. Although monocytes/macrophages from patients with VL have demonstrated an impaired oxidative burst and antigen presentation, an unanswered yet pertinent question remains ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12535
更新日期:2018-07-01 00:00:00
abstract::Culex pipiens and Aedes aegypti mosquitoes were fed on C3H/HeJ mice and systemic cytokine production was quantified from stimulated lymphocytes harvested four to ten days after feeding. Mosquito feeding on C3H/HeJ mice significantly down regulated IFN gamma production seven to ten days post feeding by Cx. pipiens and ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1999.00199.x
更新日期:1999-01-01 00:00:00
abstract::Changes in phagocytic and adherent cell numbers were compared during the course of infections of mice with Plasmodium yoelii (Py) and P. berghei (Pb) and in vaccinated mice challenged with homologous parasites. Nucleated cells in the spleen increased in number in Py-infected mice and were maximal at the time of recove...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1979.tb00696.x
更新日期:1979-04-01 00:00:00
abstract::The pathogenesis of the tissue lesions observed in American trypanosomiasis (Chagas' disease) seems to depend on a variety of mechanisms. The present study was dedicated to the investigation of the evolution of muscle lesions and its relationship with immunological and parasitological parameters. Mice were infected i....
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1985.tb00091.x
更新日期:1985-09-01 00:00:00
abstract::Longitudinal cohort studies are important to describe the dynamics of naturally acquired antibody response profiles to defined Plasmodium falciparum malaria antigens relative to clinical malaria episodes. In children under 7 years of age in The Gambia, serum IgG responses were measured to P. falciparum merozoite antig...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2009.01193.x
更新日期:2010-04-01 00:00:00
abstract::Toxoplasma gondii-specific murine CD4+ T cell clone 3Tx9 belongs to the Th1 subtype by virtue of secreting high levels of interleukin(IL)-2, interferon-gamma and tumor necrosis factor without producing IL4 and IL10. To characterize the clonal antigen, Toxoplasma lysate-was separated by SDS-PAGE and probed in T cell bl...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1997.d01-201.x
更新日期:1997-05-01 00:00:00
abstract::The efficacy of vaccination against Haemonchus contortus infection with two recombinant proteins, rHco-gal-m and rHco-gal-f, was studied in 9-10-month-old goats. Vaccination with 100 microg protein reduced faecal egg output and worm burdens by 37.25% and 41.1%, respectively. Corresponding reductions with 200 microg pr...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2007.00949.x
更新日期:2007-06-01 00:00:00
abstract::The present paper reviews the available literature on the development of immunity to animal Schistosoma infections. The majority of the studies on animal schistosomiasis were performed in cattle and pigs and only Schistosoma mattheei, S. bovis and S. japonicum received particular attention, mainly because of their rec...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-3024.2005.00766.x
更新日期:2005-07-01 00:00:00
abstract::Onchocerca volvulus and the human immunodeficiency virus (HIV) are two immunocompromising infectious agents of major public health concern in Uganda. To examine the effect of coinfection with O. volvulus and HIV on cellular immune responses, lymphocyte proliferative responses and cytokine production of peripheral bloo...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1998.00170.x
更新日期:1998-09-01 00:00:00
abstract::Very little has been reported comparing resistance to coccidiosis in fast or slow growing broilers, the latter of which are becoming more prevalent in the broiler industry. We examined mRNA expression in the intestines of fast and slow growing broilers following Eimeria infection. We show that by day 13 post-infection...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12660
更新日期:2019-09-01 00:00:00
abstract::Plasmodium falciparum-specific antibodies tend to be short-lived, but their cognate memory B cells (MBCs) circulate in the peripheral blood of exposed subjects for several months or years after the last infection. However, the time course of antigen-specific antibodies and B-cell responses to the relatively neglected ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12662
更新日期:2019-09-01 00:00:00
abstract::DNA immunization represents a promising vaccine strategy that has been reasonably successful, and will likely play an even greater role in vaccine development as these vaccines continue to be improved. We have developed a partially protective DNA vaccine against schistosome infection based on a 23-kDa integral membran...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2003.00651.x
更新日期:2003-08-01 00:00:00
abstract::Mice infected with lethal P. berghei or P. yoelii malaria develop anti-lymphocyte autoantibodies less than a week after infection. The autoantibodies are IgM, T-dependent and cytotoxic to a subpopulation of non-T lymphocytes at 37 degrees C in the presence of mouse or guinea-pig complement. Non-lethal P. yoelii and P....
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1983.tb00742.x
更新日期:1983-05-01 00:00:00