Abstract:
:In this study we have investigated the ability of the stage-specific monoclonal antibody (MoAb) 1G7 to neutralize the infectivity of Trypanosoma cruzi metacyclic trypomastigotes (Tulahuen strain). Mice inoculated with metacyclics pre-incubated with 1G7 or the corresponding Fab fragments were found to develop greatly reduced parasitaemias compared with control animals. Similar results were obtained when mice received 1G7 (0.5 mg/mouse) before challenge with metacyclics. The 90 kD surface protein recognized by MoAb 1G7 was affinity purified and analysed for its immunogenic properties. Immunization of mice with 90 kD antigen induced antibodies that reacted with the native metacyclic surface antigen. The purified antigen was also capable of specifically stimulating the 'in-vitro' proliferation of 90 kD-primed T-cells. Furthermore, in a preliminary experiment in which mice were immunized with the purified polypeptide and challenged with Tulahuen metacyclics, low parasitaemia levels were observed in 60% of animals as opposed to non-immunized controls. The iodinated 90 kD antigen, which has previously been shown to be ubiquitous among T. cruzi strains, was precipitated by different immune sera including various chagasic sera.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Araguth MF,Rodrigues MM,Yoshida Ndoi
10.1111/j.1365-3024.1988.tb00256.xsubject
Has Abstractpub_date
1988-11-01 00:00:00pages
707-12issue
6eissn
0141-9838issn
1365-3024journal_volume
10pub_type
杂志文章abstract::A 120 kDa antigen containing SDS-PAGE fraction BmA-2 isolated from Brugia malayi adult parasite was highly reactive with normal sera from filarial endemic area. BmA-2 was analysed for its prophylactic potential in in vitro and in vivo. Sera collected from BmA-2 immunized jirds induced a significant level (80 to 90%) o...
journal_title:Parasite immunology
pub_type: 杂志文章
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journal_title:Parasite immunology
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1996-12-01 00:00:00
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journal_title:Parasite immunology
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journal_title:Parasite immunology
pub_type: 杂志文章,评审
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journal_title:Parasite immunology
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journal_title:Parasite immunology
pub_type: 杂志文章,评审
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更新日期:2007-12-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1994.tb00305.x
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:2000-02-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1992-11-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1996.d01-17.x
更新日期:1996-10-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1993.tb00592.x
更新日期:1993-03-01 00:00:00
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journal_title:Parasite immunology
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更新日期:2002-02-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.0141-9838.2004.00671.x
更新日期:2003-11-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1988.tb00205.x
更新日期:1988-01-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章,随机对照试验
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1996.tb01028.x
更新日期:1996-09-01 00:00:00
abstract::Infection with Trypanosoma cruzi was established in the reduviid vector, Dipetalogaster maximus, by repeated feedings on mice with high parasitaemias, and metacyclic trypomastigotes (IMT) were collected in insect urine after blood meals. The infectivity of IMT in mice was assessed by placing varying numbers of organis...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1990.tb00936.x
更新日期:1990-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2018-09-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1986.tb00874.x
更新日期:1986-11-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:2020-10-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1994.tb00367.x
更新日期:1994-08-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1988.tb00242.x
更新日期:1988-09-01 00:00:00
abstract::Trypanosoma congolense is one of the main species responsible for Animal African Trypanosomosis (AAT). As preventive vaccination strategies for AAT have been unsuccessful so far, investigating the mechanisms underlying vaccine failure has to be prioritized. In T. brucei and T. vivax infections, recent studies revealed...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12099
更新日期:2014-05-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1981.tb00405.x
更新日期:1981-10-01 00:00:00
abstract::Carboxy-terminus of merozoite surface protein-1 (MSP-1(19) ) is the major protein on the surface of the plasmodial merozoite that acts as one of the most important blood-stage vaccine candidates. The present investigation was designed to evaluate the immune responses when either two recombinant antigens (rPvMSP-1(19) ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2011.01331.x
更新日期:2011-11-01 00:00:00