Abstract:
:Sheep scab is caused by the noninvasive mite, Psoroptes ovis, which initiates a profound pro-inflammatory skin response leading to lesion development. To investigate these early events between the skin and the parasite, primary ovine epidermal keratinocyte cultures were generated and challenged with mite derived antigens. The kinetics of the mRNA response of these cells were monitored by microarray. The results indicated that the cells responded within 1 h of challenge, with a significant increase in the pro-inflammatory cytokine IL-8. This result was confirmed by real-time RT-PCR, and showed that IL-8 up-regulation was maximal at 1 h but declined to pre-stimulation levels at 24 and 48 h. The IL-8 mRNA response to mite wash antigens containing secretory and/or excretory proteins was also investigated and compared to the response to whole mite antigen. These studies revealed that the mite wash antigen, at a challenge dose of 10 microg/mL, was markedly more potent and induced significantly higher levels of IL-8 mRNA than the same concentration of whole mite antigen. These results are discussed in relation to mite establishment and survival on the ovine host.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Watkins CA,Mackellar A,Frew D,Mackie C,George A,Hopkins J,Burgess ST,McNeilly TN,Huntley JFdoi
10.1111/j.1365-3024.2009.01103.xsubject
Has Abstractpub_date
2009-06-01 00:00:00pages
304-11issue
6eissn
0141-9838issn
1365-3024pii
PIM1103journal_volume
31pub_type
杂志文章abstract::Immunization of mammals with irradiated malaria sporozoites protects from a subsequent contact with the parasite. Protective immunity is directed against the pre-erythrocytic stages of the parasite, sporozoites and liver stages. Specific antibodies neutralize part of the infectious sporozoites infected by the mosquito...
journal_title:Parasite immunology
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journal_title:Parasite immunology
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journal_title:Parasite immunology
pub_type: 杂志文章
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1996.d01-62.x
更新日期:1996-03-01 00:00:00
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journal_title:Parasite immunology
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journal_title:Parasite immunology
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journal_title:Parasite immunology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1111/pim.12317
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journal_title:Parasite immunology
pub_type: 杂志文章,评审
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更新日期:1996-06-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1988-11-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1987.tb00487.x
更新日期:1987-01-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1996-02-01 00:00:00