Abstract:
:In the present study, the cytokines interleukin (IL)-12 and IL-18 were evaluated for their capacity to modulate and to re-direct in vitro parasite antigen-specific cellular responsiveness in patients exposed to Onchocerca volvulus and Entamoeba histolytica infection. We found that IL-18 was highly capable of reducing parasite antigen-induced IL-10 production by PBMC. In contrast, addition or neutralization of IL-12, also in combination with IL-18 and the interferon-gamma-inducible chemokine IP-10 did not affect IL-10 production. Interestingly, the highest IL-10 levels were measured when IL-18 and IP-10 were both neutralized. Although having no effect on IL-10, IL-12 strongly promoted spontaneous and parasite antigen-driven IFN-gamma production by PBMC, whereas IL-18 was only moderately affecting IFN-gamma release by PBMC re-stimulated with E. histolytica- or O. volvulus-specific antigens. Both IL-12 and IL-18 diminished the cellular production of IL-13, and a synergistic effect was observed when the cytokines were combined. Likewise, neutralization of IL-12 enhanced Entamoeba and Onchocerca antigen-driven IL-13 production, but no further increase of IL-13 was observed, when anti-IL-12 and anti-IL-18 were used together. This study disclosed that IL-18 will significantly down-regulate parasite-specific IL-10 production, whereas IL-12 induced IFN-gamma and inhibited IL-13 production by PBMC from humans exposed to O. volvulus and E. histolytica. Such selective immune-regulatory capacity of IL-12 and IL-18 may comprise an important tool to re-direct polarized cytokine responses towards a balanced Th1/Th2 cytokine profile, which may prevent pathology and promote immunity against helminth and protozoan parasite infections.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Pfaff AW,Kirch AK,Hoffmann WH,Banla M,Schulz-Key H,Geiger SM,Soboslay PTdoi
10.1046/j.1365-3024.2003.00638.xkeywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
325-32issue
6eissn
0141-9838issn
1365-3024pii
638journal_volume
25pub_type
杂志文章abstract:AIMS:Schistosomiasis and malaria are endemic in sub-Saharan Africa where Schistosoma haematobium (Sh) and Plasmodium falciparum (Pf) coinfections are thus frequent. We explored the effect of Sh infection on antibody responses directed to Pf merozoite antigens and on malaria susceptibility in Beninese children. METHODS...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12702
更新日期:2020-04-01 00:00:00
abstract::The soluble portion of a large particle fraction which was derived from the muscle larva of T. spiralis was subjected to molecular sizing column chromatography using Sephacryl S-200. Five major peaks of 280 nm absorbing material were obtained. Analysis by immunoelectrophoresis revealed that each peak contained antigen...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1981.tb00405.x
更新日期:1981-10-01 00:00:00
abstract::Specific CD8(-) T-lymphocyte (CTL) activity against Plasmodium pre-erythrocytic stages (P-ES) derived antigens is considered one of the most important mechanisms for malaria protection. Plasmodium vivax is the second most prevalent human malaria parasite species distributed worldwide. Although several CTL epitopes hav...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2002.00449.x
更新日期:2002-03-01 00:00:00
abstract::SUMMARY Previous experiments have shown that genetic resistance to infection by Teladorsagia circumcincta in sheep can be measured by the level of IgA in gastric mucus, jointly with other parameters. The aim of this study has been to observe the influence of IgA on adult worms. The experiment was carried out with Chur...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2005.00769.x
更新日期:2005-06-01 00:00:00
abstract::Haemonchus contortus is an economically important parasite that survives the host defense system by modulating the immune response. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is secreted by the parasite and the host responds by producing anti-enzyme antibodies. The enzyme inhibits complement cascade, an arm of t...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12611
更新日期:2019-02-01 00:00:00
abstract::Hyporesponsiveness induced by Heligmosomoides polygyrus was quantified and the relationship between TGF-beta and inflammation was identified in BALB/c mice. The immune response and pathological changes modified by neutralization of TGF-beta were characterized in vivo. Nine and twelve days following infection, BALB/c m...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2006.00845.x
更新日期:2006-08-01 00:00:00
abstract::In order to gain insights into the immune response in onchocerciasis during early infection, laboratory-reared calves were infected with 1000 Onchocerca lienalis infective larvae and examined serologically over a period of 508 days. Levels of serum antibodies measured by ELISA against adult worm extract revealed a mul...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1995.tb00907.x
更新日期:1995-08-01 00:00:00
abstract::The previously observed MHC-restriction of the antibody response to an 86kDa S. mansoni antigen has been investigated in more detail. The I-A locus of the H-2 complex has been implicated as conferring responder or non-responder status on mice expressing the k and b alleles respectively. Inheritance of responsiveness w...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1991.tb00281.x
更新日期:1991-05-01 00:00:00
abstract::Immunity to Schistosoma mansoni in the mouse was induced by vaccination with adult worm surface membrane (mb-S). Of several adjuvants tested, including Freund's, BCG and alum, 50 micrograms of saponin per mouse given subcutaneously with the antigen was the easiest to administer, and gave consistent protection, approac...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1989.tb00669.x
更新日期:1989-07-01 00:00:00
abstract::Resistance to S. mansoni cercarial challenge, induced by prior infection of rats with cercariae, is analysed by substitution of alternatives to the primary cercarial exposure. Intravenous injections of either live or killed 4-week stage worms have been examined, utilizing two different routes of injection, one or two ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1984.tb00790.x
更新日期:1984-03-01 00:00:00
abstract::Chronic Chagas cardiomyopathy (CCC) is responsible for the disease's greater morbidity and poor prognosis. Although understanding the pathophysiology of CCC and the fundamentals of its clinical management derives from research related to other cardiomyopathies, there are peculiarities that distinguish CCC from the oth...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12663
更新日期:2019-10-01 00:00:00
abstract::Cryptosporidiosis is a zoonotic protozoan disease that affects the gastrointestinal tract of animals and humans. Diarrhoea as the most important indication of the infection leads to high economic losses in livestock industries and is a life threatening infection in immunocompromised individuals. In the absence of the ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12317
更新日期:2016-05-01 00:00:00
abstract::Infective forms of Trypanosoma cruzi, the parasite that causes Chagas' disease, express on their surface an enzyme denominated trans-sialidase (TS). The present study was designed to evaluate the naturally acquired immune responses to a bacterial recombinant protein representing the catalytic domain of TS in chronical...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2000.00260.x
更新日期:2000-01-01 00:00:00
abstract::Malaria remains one of the most serious health problems globally, but our understanding of the biology of the parasite and the pathogenesis of severe disease is still limited. Multiple cellular effector mechanisms that mediate parasite elimination from the liver have been described, but how effector cells use classica...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12039
更新日期:2013-09-01 00:00:00
abstract::The in vitro binding serum complement (C) components to Schistosoma mansoni worms was studied. By exposing frozen sections of adult male and female parasites to normal human serum, binding of both classical and alternative pathway C components was observed. By immunofluorescence (IFL) microscopy selective binding of C...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1983.tb00736.x
更新日期:1983-03-01 00:00:00
abstract::Hookworms are one of the most prevalent parasites of humans in developing countries, but we know relatively little about the immune response generated to hookworm infection. This can be attributed to a lack of permissive animal models and a relatively small research community compared with those of the more high-profi...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-3024.2010.01224.x
更新日期:2010-08-01 00:00:00
abstract::Immunization of mammals with irradiated malaria sporozoites protects from a subsequent contact with the parasite. Protective immunity is directed against the pre-erythrocytic stages of the parasite, sporozoites and liver stages. Specific antibodies neutralize part of the infectious sporozoites infected by the mosquito...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1997.d01-190.x
更新日期:1997-03-01 00:00:00
abstract::Leishmania enter macrophages through receptor-mediated phagocytosis and survive the harsh environment of a phagolysosome. Here, we investigated the interaction between mannose receptor (MR), Toll-like receptor 2 (TLR2), and Leishmania, and the subsequent impact on phagosome maturation. Leishmania parasites are able to...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12521
更新日期:2018-04-01 00:00:00
abstract::Attempts to produce monoclonal antibodies specific for a parasite-encoded or induced antigen on the surface of Theileria parva lawrencei-transformed bovine lymphocytes resulted in the identification of the monoclonal antibody J7. This antibody recognizes a membrane glycoprotein on some T.p. lawrencei-infected bovine l...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1986.tb00841.x
更新日期:1986-03-01 00:00:00
abstract::Immune responses of 97 Gambian women and their neonates were studied. New methods distinguished between active and previous placental malaria, were used to examine relationships between maternal malaria and neonatal immune responses. Many placentas (61%) had active or previous malarial infection. Maternal and cord mal...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1995.tb00960.x
更新日期:1995-01-01 00:00:00
abstract::Intestinal helminth infections are highly prevalent in the developing world, often resulting in chronic infection and inflicting high host morbidity. With the emergence of drug-resistant parasites, a limited number of chemotherapeutic drugs available and stalling vaccine efforts, an increased understanding of antihelm...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12406
更新日期:2017-02-01 00:00:00
abstract::We describe an ELISA for trophozoite-specific IgA in the intestinal secretions of mice infected with Giardia muris. Using this method, trophozoite-specific IgA was demonstrated in intestinal secretions of Giardia-infected immunocompetent BALB/c mice. Such antibody was undetectable in the intestinal secretions of Giard...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1988.tb00257.x
更新日期:1988-11-01 00:00:00
abstract::The role of autoimmunity in the pathogenesis and progression of heart lesions in the chronic phase of Chagas' disease is controversial. In the absence of parasites in situ, the T cell infiltrate seen in heart lesions may be the primary determinant of tissue damage ultimately leading to heart failure and death. We used...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1994.tb00337.x
更新日期:1994-04-01 00:00:00
abstract::To determine the role of T cells and mast cells in intestinal pathology and immune expulsion of intestinal nematodes, worm burdens, goblet cell responses and villus structures were analysed in T- and B-cell-deficient severe combined immunodeficiency (SCID) mice, athymic nu/nu mice and mast cell deficient W/W(v) mice a...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2009.01123.x
更新日期:2009-08-01 00:00:00
abstract::Cryptocaryon irritans is one of the most important ectoparasites of marine fish. To identify the potential role of immune-related genes in antiparasitic immune responses in fish, we monitored the expression change of IL-8, COX-2, C-type lectin and transferrin in local and systemic immune organs of orange-spotted group...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2011.01337.x
更新日期:2011-12-01 00:00:00
abstract::Studies in animal models suggest that protection against malaria induced by intradermal (ID) administration of sporozoites is less effective compared to intravenous injection (IV). We investigated in a murine model the protective efficacy and immune responses after ID or IV immunization of sporozoites. Mice were immun...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12000.x
更新日期:2012-12-01 00:00:00
abstract::Immunoprecipitations of in vitro translation products of mRNA from adult Schistosoma mansoni worms with sera from chronically infected mice of 12 inbred strains reveal a strain-dependent heterogeneity in the antibody responses to schistosome antigens. Of the strains tested the response to the 86K antigen was restricte...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1986.tb00870.x
更新日期:1986-11-01 00:00:00
abstract::The present study was designed to investigate the role of neutrophils during the development of Schistosoma japonicum egg granulomas, in C57BL/6 and CBA mice. Laid eggs were implanted into the liver and monoclonal antibody, RB6-8C5, was used to eliminate neutrophils. After daily antibody treatment between days 9 and 1...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2002.00491.x
更新日期:2002-09-01 00:00:00
abstract::Chagas' disease or American trypanosomiasis due to Trypanosoma cruzi has existed at least since the time of the Inca empire and contributes significantly to cardiovascular morbidity and mortality in several countries of this continent. Due to the fundamental role of human class II molecules polymorphic residues in the...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::The role of CD4+ and CD8+ T cells in protective immunity to Trichuris muris was studied in CD4+ or CD8+ or both CD4+ and CD8+ T cell-depleted BALB/c mice. To assess in vivo depletion of T-cell subsets, CD4+ and CD8+ T cells in the Peyer's patches, the mesenteric lymph nodes (MLN), and the spleens of mice treated with ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1995.tb01018.x
更新日期:1995-03-01 00:00:00