Abstract:
:Recently, several groups have initiated studies on cytosolic proteins that bind to isolated secretory vesicle membranes in the presence of Ca2+ in order to identify proteins that may regulate exocytosis. Two major chromaffin granule binding proteins, of molecular weights 32,000 (32K) and 34,000 (34K), were reported to have the same mobility on one-dimensional SDS gels as clathrin-associated light chains from the adrenal medulla, and the 34K granule binding protein the same one-dimensional peptide map as the 34K clathrin light chain. These observations support the hypothesis that Ca2+-dependent recruitment of soluble light chains to the vesicle membrane may nucleate the assembly of a clathrin coat and initiate endocytosis. Here we report that two-dimensional peptide maps of the clathrin light chains and of all chromaffin granule membrane binding proteins in the 30K range are distinct, and therefore fail to support this hypothesis. It has also been suggested that some or all of the vesicle binding proteins require calmodulin for their interaction with the membrane. However, we find that antagonism of calmodulin by trifluoperazine does not prevent the association of the other cytosolic proteins with the chromaffin granule membrane.
journal_name
Naturejournal_title
Natureauthors
Creutz CE,Harrison JRdoi
10.1038/308208a0subject
Has Abstractpub_date
1984-03-08 00:00:00pages
208-10issue
5955eissn
0028-0836issn
1476-4687journal_volume
308pub_type
杂志文章相关文献
NATURE文献大全abstract::The study of language origin and divergence is important for understanding the history of human populations and their cultures. The Sino-Tibetan language family is the second largest in the world after Indo-European, and there is a long-running debate about its phylogeny and the time depth of its original divergence1....
journal_title:Nature
pub_type: 历史文章,杂志文章
doi:10.1038/s41586-019-1153-z
更新日期:2019-05-01 00:00:00
abstract::Double-stranded RNAs (dsRNAs) induce post-transcriptional gene silencing in several species of animal and plant. In plants, dsRNAs targeted to CpG islands within a promoter can also induce RNA-directed DNA methylation; however, it remains unclear whether gene silencing mediated by DNA methylation can be induced by dsR...
journal_title:Nature
pub_type: 杂志文章,收录出版
doi:10.1038/nature02889
更新日期:2004-09-09 00:00:00
abstract::Topological physics relies on the structure of the eigenstates of the Hamiltonians. The geometry of the eigenstates is encoded in the quantum geometric tensor1-comprising the Berry curvature2 (crucial for topological matter)3 and the quantum metric4, which defines the distance between the eigenstates. Knowledge of the...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-1989-2
更新日期:2020-02-01 00:00:00
abstract::Seafloor spreading is largely unobserved because 98 per cent of the global mid-ocean-ridge system is below the ocean surface. Our understanding of the dynamic processes that control seafloor spreading is thus inferred largely from geophysical observations of spreading events on land at Afar in East Africa and Iceland....
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature20116
更新日期:2016-12-08 00:00:00
abstract::A powerful way to discover key genes with causal roles in oncogenesis is to identify genomic regions that undergo frequent alteration in human cancers. Here we present high-resolution analyses of somatic copy-number alterations (SCNAs) from 3,131 cancer specimens, belonging largely to 26 histological types. We identif...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature08822
更新日期:2010-02-18 00:00:00
abstract::A long-standing problem in managing the behaviour of a collection of solid grains concerns the nature of the grain packing, a property that is typically controlled by how the grains are poured or shaken. Here we show that a systematic and controllable increase in granular packing can be induced by simply raising and t...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/442257a
更新日期:2006-07-20 00:00:00
abstract::Silicon dominates contemporary solar cell technologies1. But when absorbing photons, silicon (like other semiconductors) wastes energy in excess of its bandgap2. Reducing these thermalization losses and enabling better sensitivity to light is possible by sensitizing the silicon solar cell using singlet exciton fission...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-019-1339-4
更新日期:2019-07-01 00:00:00
abstract::The rates and routes of lethal systemic spread in breast cancer are poorly understood owing to a lack of molecularly characterized patient cohorts with long-term, detailed follow-up data. Long-term follow-up is especially important for those with oestrogen-receptor (ER)-positive breast cancers, which can recur up to t...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-019-1007-8
更新日期:2019-03-01 00:00:00
abstract::The halo of the Milky Way provides a laboratory to study the properties of the shocked hot gas that is predicted by models of galaxy formation. There is observational evidence of energy injection into the halo from past activity in the nucleus of the Milky Way1-4; however, the origin of this energy (star formation or ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-2979-0
更新日期:2020-12-01 00:00:00
abstract::The way in which proteins attain and maintain their final form is of fundamental importance. Recent work has focused on the role of a set of ubiquitous proteins, termed chaperonins, in the assembly of phage and multisubunit proteins. The range of chaperonin action is unknown; they could interact with most cellular pol...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/342451a0
更新日期:1989-11-23 00:00:00
abstract::The vertical structure of the recent Arctic warming contains information about the processes governing Arctic climate trends. Graversen et al. argue, on the basis of ERA-40 reanalysis data, that a distinct maximum in 1979-2001 warm-season (April-October) Arctic temperature trends appears around 3 km above ground. Here...
journal_title:Nature
pub_type: 评论,杂志文章
doi:10.1038/nature07257
更新日期:2008-09-11 00:00:00
abstract::Courtship is an innate sexually dimorphic behaviour that can be observed in naive animals without previous learning or experience, suggesting that the neural circuits that mediate this behaviour are developmentally programmed. In Drosophila, courtship involves a complex yet stereotyped array of dimorphic behaviours th...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature06808
更新日期:2008-03-27 00:00:00
abstract::Contact inhibition of locomotion was discovered by Abercrombie more than 50 years ago and describes the behaviour of fibroblast cells confronting each other in vitro, where they retract their protrusions and change direction on contact. Its failure was suggested to contribute to malignant invasion. However, the molecu...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature07441
更新日期:2008-12-18 00:00:00
abstract::In Drosophila, Decapentaplegic (Dpp) and Wingless (Wg) are two secreted signalling proteins of the transforming growth factor (TGF)-beta and Wnt families, respectively. Although both are often required during development, only a few downstream components of these signalling pathways have been described. Here we presen...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/383630a0
更新日期:1996-10-17 00:00:00
abstract::The limited ranges of the wild progenitors of many of the primary European domestic species point to their origins further east in Anatolia or the fertile crescent. The wild ox (Bos primigenius), however, ranged widely and it is unknown whether it was domesticated within Europe as one feature of a local contribution t...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35074088
更新日期:2001-04-26 00:00:00
abstract::Cryptochromes are blue/ultraviolet-A light receptors that mediate various light responses in plants and animals. But the initial photochemical reaction of cryptochrome is still unclear. For example, although most photoreceptors are known to undergo light-dependent protein modification such as phosphorylation, no blue-...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature00815
更新日期:2002-06-13 00:00:00
abstract::Excitatory amino acids mediate fast synaptic transmission in the central nervous system through the activation of at least three distinct ionotropic receptors: N-methyl-D-aspartate (NMDA), the alpha-amino-3-hydroxy-5-methyl-isoxasole-4-propionate (AMPA)/quisqualate (QUIS) and the kainate subtypes (for reviews, see ref...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/347765a0
更新日期:1990-10-25 00:00:00
abstract::Observations from cultured cells, animal models and patients raise the possibility that the dependency of tumours on the therapeutic drugs to which they have acquired resistance represents a vulnerability with potential applications in cancer treatment. However, for this drug addiction trait to become of clinical inte...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature24037
更新日期:2017-10-12 00:00:00
abstract::A characteristic feature of induced pinocytosis in Amoeba proteus is the formation of broad channels by invagination of the cell membrane. This process, which requires Ca2+, occurs in response to depolarising cations. High Ca2+ levels reduce pinocytosis induced by cations such as Na+ and Tris+, whereas pinocytosis ind...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/282078a0
更新日期:1979-11-01 00:00:00
abstract::Although the influence of electrical activity on neural development has been studied extensively, experiments have only recently focused on the role of activity in the development of the mammalian central nervous system (CNS). Using tetrodotoxin (TTX) to abolish sodium-mediated action potentials, studies on the visual...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/323156a0
更新日期:1986-09-11 00:00:00
abstract::Chemotaxis in Caenorhabditis elegans, like chemotaxis in bacteria, involves a random walk biased by the time derivative of attractant concentration, but how the derivative is computed is unknown. Laser ablations have shown that the strongest deficits in chemotaxis to salts are obtained when the ASE chemosensory neuron...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature06927
更新日期:2008-07-03 00:00:00
abstract::The double helix of DNA epitomizes this molecule's ability to self-assemble in aqueous solutions into a complex chiral structure using hydrogen bonding and hydrophobic interactions. Non-covalently interacting molecules in organic solvents are used to design systems that similarly form controlled architectures. Periphe...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35025027
更新日期:2000-09-14 00:00:00
abstract::More than twenty types of retinal ganglion cells conduct visual information from the eye to the rest of the brain. Each retinal ganglion cell type tessellates the retina in a regular mosaic, so that every point in visual space is processed for visual primitives such as contrast and motion. This information flows to tw...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature14103
更新日期:2015-03-12 00:00:00
abstract::Microtubules have a central role in eukaryotic cell polarity, in part through interactions between microtubule end-binding proteins and the cell cortex. In the fission yeast Schizosaccharomyces pombe, microtubules and the polarity modulator tea1p maintain cylindrical cell shape and strictly antipodal cell growth. The ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature01672
更新日期:2003-06-05 00:00:00
abstract:: ...
journal_title:Nature
pub_type: 面试
doi:10.1038/d41586-018-07198-z
更新日期:2018-11-01 00:00:00
abstract::Oligonucleotide and complementary DNA microarrays are being used to subclassify histologically similar tumours, monitor disease progress, and individualize treatment regimens. However, extracting new biological insight from high-throughput genomic studies of human diseases is a challenge, limited by difficulties in re...
journal_title:Nature
pub_type: 临床试验,杂志文章
doi:10.1038/nature03985
更新日期:2005-10-13 00:00:00
abstract::Understanding brain circuits begins with an appreciation of their component parts - the cells. Although GABAergic interneurons are a minority population within the brain, they are crucial for the control of inhibition. Determining the diversity of these interneurons has been a central goal of neurobiologists, but this...
journal_title:Nature
pub_type: 杂志文章,评审
doi:10.1038/nature12983
更新日期:2014-01-16 00:00:00
abstract::In myelinated nerves, segregation of voltage-dependent sodium channels to nodes of Ranvier is crucial for saltatory conduction along axons. As sodium channels associate and colocalize with ankyrin at nodes of Ranvier, one possibility is that sodium channels are recruited and immobilized at axonal sites which are speci...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/356333a0
更新日期:1992-03-26 00:00:00
abstract::Matrix metalloproteinases (MMPs) are zinc endopeptidases that are required for the degradation of extracellular matrix components during normal embryo development, morphogenesis and tissue remodelling. Their proteolytic activities are precisely regulated by endogenous tissue inhibitors of metalloproteinases (TIMPs). D...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/37995
更新日期:1997-09-04 00:00:00
abstract::Mice express a collection of superantigens, which bind to class II major histocompatibility proteins and interact with T cells bearing particular V beta chains as part of their alpha beta receptors. These superantigens have been suggested to be encoded by exogenous or endogenous mouse mammary tumour viruses. One such ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/350203a0
更新日期:1991-03-21 00:00:00