Absence of detectable IgM in enzymatically or biosynthetically labeled thymus-derived lymphocytes.

abstract：:Up-regulation of the folding machinery of the heat-shock protein 90 (Hsp90) chaperone protein is crucial for cancer progression. The two Hsp90 isoforms (α and β) play different roles in response to chemotherapy. To identify isoform-selective inhibitors of Hsp90(α/β)/cochaperone p23 interactions, we developed a dual-lu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chan CT,Reeves RE,Geller R,Yaghoubi SS,Hoehne A,Solow-Cordero DE,Chiosis G,Massoud TF,Paulmurugan R,Gambhir SS

更新日期：2012-09-11 00:00:00

abstract：:DNA was prepared from 15 different mouse and rat cell lines transformed by chemical carcinogens in vitro and in vivo. These DNAs were applied to NIH3T3 mouse fibroblast cultures by using the calcium phosphate transfection technique. DNAs of five donor lines were able to induce foci on the recipient monolayers. Ten oth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shih C,Shilo BZ,Goldfarb MP,Dannenberg A,Weinberg RA

更新日期：1979-11-01 00:00:00

abstract：:Phage phi 29 terminal protein, p3, essentially pure, was isolated in a denatured form from viral particles, and anti-p3 antiserum was obtained. A radioimmunoassay to detect and quantitate protein p3 was developed. By using this assay, native protein p3 was highly purified from Escherichia coli cells harboring a gene 3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prieto I,Lázaro JM,García JA,Hermoso JM,Salas M

更新日期：1984-03-01 00:00:00

abstract：:A de novo mutation in the KCND2 gene, which encodes the Kv4.2 K+ channel, was identified in twin boys with intractable, infant-onset epilepsy and autism. Kv4.2 channels undergo closed-state inactivation (CSI), a mechanism by which channels inactivate without opening during subthreshold depolarizations. CSI dynamically...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin MA,Cannon SC,Papazian DM

更新日期：2018-04-10 00:00:00

abstract：:In 1989, populations of North American gypsy moth, Lymantria dispar, in seven contiguous northeastern states were severely reduced by a fungal pathogen. Based on morphology, development, and pathology, this organism appeared to be Entomophaga maimaiga. We have now used allozyme and restriction fragment length polymorp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hajek AE,Humber RA,Elkinton JS,May B,Walsh SR,Silver JC

更新日期：1990-09-01 00:00:00

abstract：:The gluconate (gnt) operon of Bacillus subtilis consists of four gnt genes; the second and third genes code for gluconate kinase (gluconokinase, EC 2.7.1.12) and gluconate permease, respectively. A fragment carrying the promoter of this operon (gnt promoter) and the first gene (gntR) was subcloned into a promoter prob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fujita Y,Fujita T

更新日期：1987-07-01 00:00:00

abstract：:Multiple resistance and pH adaptation (Mrp) complexes are sophisticated cation/proton exchangers found in a vast variety of alkaliphilic and/or halophilic microorganisms, and are critical for their survival in highly challenging environments. This family of antiporters is likely to represent the ancestor of cation pum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li B,Zhang K,Nie Y,Wang X,Zhao Y,Zhang XC,Wu XL

更新日期：2020-12-08 00:00:00

abstract：:RNA was synthesized in vitro from a T3 DNA template by T3 RNA polymerase and subsequently separated into seven discrete size classes (molecular weights ranging between 0.21 x 10(6) and 6.2 x 10(6)) by electrophoresis in polyacrylamide slab gels. RNase T1-generated 3'-terminal oligonucleotide fragments were then select...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Majumder HK,Maitra U,Rosenberg M

更新日期：1979-10-01 00:00:00

abstract：:VASP (vasodilator-stimulated phosphoprotein), an established substrate of cAMP- and cGMP-dependent protein kinases in vitro and in living cells, is associated with focal adhesions, microfilaments, and membrane regions of high dynamic activity. Here, the identification of an 83-kDa protein (p83) that specifically binds...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reinhard M,Jouvenal K,Tripier D,Walter U

更新日期：1995-08-15 00:00:00

abstract：:We are investigating the feasibility of using the positive-strand RNA virus Sindbis virus and its defective interfering (DI) particles as vectors for introducing foreign genes into cells. In previous work we showed by deletion mapping of a cloned cDNA derived from one of the DI RNAs that only nucleotides at the 3' and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levis R,Huang H,Schlesinger S

更新日期：1987-07-01 00:00:00

abstract：:Cellular telomere length is linked to replicative life span. Telomere repeats are lost in peripheral blood cells in vivo by age, and women show less telomere attrition than men. Previous reports have indicated that telomere length and chromosome-specific telomere-length patterns partly are inherited. The mode of hered...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nordfjäll K,Larefalk A,Lindgren P,Holmberg D,Roos G

更新日期：2005-11-08 00:00:00

abstract：:Mitochondrial ATP-sensitive K (mitoK(ATP)) channels play a central role in protecting the heart from injury in ischemic preconditioning. In isolated mitochondria exposed to elevated extramitochondrial Ca, P(i), and anoxia to simulate ischemic conditions, the selective mitoK(ATP) channel agonist diazoxide (25-50 microM...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Korge P,Honda HM,Weiss JN

更新日期：2002-03-05 00:00:00

abstract：:For many years, the Neanderthals have been recognized as a distinctive extinct hominid group that occupied Europe and western Asia between about 200,000 and 30,000 years ago. It is still debated, however, whether these hominids belong in their own species, Homo neanderthalensis, or represent an extinct variant of Homo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwartz JH,Tattersall I

更新日期：1996-10-01 00:00:00

abstract：:Remembering is a complex process that involves recalling specific details, such as who you were with when you celebrated your last birthday, as well as contextual information, such as the place where you celebrated. It is well established that the act of remembering enhances long-term retention of the retrieved inform...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jonker TR,Dimsdale-Zucker H,Ritchey M,Clarke A,Ranganath C

更新日期：2018-10-23 00:00:00

abstract：:Noonan syndrome (NS) is a relatively common genetic disorder, characterized by typical facies, short stature, developmental delay, and cardiac abnormalities. Known causative genes account for 70-80% of clinically diagnosed NS patients, but the genetic basis for the remaining 20-30% of cases is unknown. We performed ne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen PC,Yin J,Yu HW,Yuan T,Fernandez M,Yung CK,Trinh QM,Peltekova VD,Reid JG,Tworog-Dube E,Morgan MB,Muzny DM,Stein L,McPherson JD,Roberts AE,Gibbs RA,Neel BG,Kucherlapati R

更新日期：2014-08-05 00:00:00

abstract：:Rod and cone opsin genes are expressed in a mutually exclusive manner in their respective photoreceptor subtypes in the mammalian retina. Previous transgenic mouse studies showed that functional interactions between the distal enhancer and proximal promoter of rhodopsin and long/medium-wavelength (L/M) opsin genes are...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peng GH,Chen S

更新日期：2011-10-25 00:00:00

abstract：:The assembly of the three neuronal soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins synaptobrevin 2, syntaxin-1A, and SNAP-25 is the key step that leads to exocytotic fusion of synaptic vesicles. In the fully assembled SNARE complex, these three proteins form a coiled-coil ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang B,Kiessling V,Tamm LK

更新日期：2013-11-26 00:00:00

abstract：:Real-world studies show that the facial expressions produced during pain and orgasm-two different and intense affective experiences-are virtually indistinguishable. However, this finding is counterintuitive, because facial expressions are widely considered to be a powerful tool for social interaction. Consequently, de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen C,Crivelli C,Garrod OGB,Schyns PG,Fernández-Dols JM,Jack RE

更新日期：2018-10-23 00:00:00

abstract：:Stimulation of the region antero-ventral to the third cerebral ventricle (AV3V) by a cholinergic drug, carbachol, and lesions of the AV3V have been demonstrated in previous studies to either augment or decrease sodium excretion, respectively. Atrial natriuretic peptide (ANP) dramatically increases renal sodium excreti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baldissera S,Menani JW,dos Santos LF,Favaretto AL,Gutkowska J,Turrin MQ,McCann SM,Antunes-Rodrigues J

更新日期：1989-12-01 00:00:00

abstract：:Transmissible spongiform encephalopathies (TSEs), or prion diseases, are mammalian neurodegenerative disorders characterized by a posttranslational conversion and brain accumulation of an insoluble, protease-resistant isoform (PrP(Sc)) of the host-encoded cellular prion protein (PrP(C)). Human and animal TSE agents ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Casalone C,Zanusso G,Acutis P,Ferrari S,Capucci L,Tagliavini F,Monaco S,Caramelli M

更新日期：2004-03-02 00:00:00

abstract：:The acid-sensing ion channel 1a (ASIC1a) is abundantly expressed in the amygdala complex and other brain regions associated with fear. Studies of mice with a disrupted ASIC1 gene suggested that ASIC1a may contribute to learned fear. To test this hypothesis, we generated mice overexpressing human ASIC1a by using the pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wemmie JA,Coryell MW,Askwith CC,Lamani E,Leonard AS,Sigmund CD,Welsh MJ

更新日期：2004-03-09 00:00:00

abstract：:In most organisms, light plays a key role in the synchronization of the circadian timing system with the environmental day-night cycle. Light pulses that phase-shift the circadian clock also induce the expression of period (per) genes in vertebrates. Here, we report the cloning of a zebrafish per gene, zfper4, which i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vallone D,Gondi SB,Whitmore D,Foulkes NS

更新日期：2004-03-23 00:00:00

abstract：:All immunoglobulins and T-cell receptors throughout phylogeny share regions of highly conserved amino acid sequence. To identify possible primitive immunoglobulins and immunoglobulin-like molecules, we utilized 3' RACE (rapid amplification of cDNA ends) and a highly conserved constant region consensus amino acid seque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berstein RM,Schluter SF,Shen S,Marchalonis JJ

更新日期：1996-04-16 00:00:00

abstract：:The traG protein of the F sex factor is an inner membrane protein with a molecular weight of 116,000. Mutants in traG (or in traN) are able to trigger conjugal DNA replication even though they cannot efficiently form stable mating aggregates with F(-) cells. The traG protein (and the traN protein) probably acts in the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manning PA,Morelli G,Achtman M

更新日期：1981-12-01 00:00:00

abstract：:Molecules of keystone significance are relatively rare, yet mediate a variety of interactions between organisms. They influence the distribution and abundance of species, the transfer of energy across multiple trophic levels, and thus they play significant roles in structuring ecosystems. Despite their potential impor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kicklighter CE,Kamio M,Nguyen L,Germann MW,Derby CD

更新日期：2011-07-12 00:00:00

abstract：:Efficient computation of geophysical turbulence, such as occurs in the atmosphere and ocean, is a formidable challenge for the following reasons: the complex combination of waves, jets, and vortices; significant energetic backscatter from unresolved small scales to resolved large scales; a lack of dynamical scale sepa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grooms I,Majda AJ

更新日期：2013-03-19 00:00:00

abstract：:In choanoflagellates, the closest living relatives of animals, multicellular rosette development is regulated by environmental bacteria. The simplicity of this evolutionarily relevant interaction provides an opportunity to identify the molecules and regulatory logic underpinning bacterial regulation of development. We...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woznica A,Cantley AM,Beemelmanns C,Freinkman E,Clardy J,King N

更新日期：2016-07-12 00:00:00

abstract：:A significant improvement in molecular hydrogen uptake properties is revealed by our ab initio calculations for Li-decorated metal-organic framework 5. We have found that two Li atoms are strongly adsorbed on the surfaces of the six-carbon rings, one on each side, carrying a charge of +0.9e per Li atom. Each Li can cl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blomqvist A,Araújo CM,Srepusharawoot P,Ahuja R

更新日期：2007-12-18 00:00:00

abstract：:We report the successful infection of two rabbit T-cell lines and one rabbit macrophage line with human immunodeficiency virus 1 (HIV-1). One T-cell line was a herpesvirus ateles transformant, the other T-cell line was a human T-cell leukemia virus I transformant, and the macrophage line was a simian virus 40 transfor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kulaga H,Folks TM,Rutledge R,Kindt TJ

更新日期：1988-06-01 00:00:00

abstract：:Rat liver nuclei pure by enzymatic and electron microscope criteria contain protein kinase C (PKC) that can be activated several hundredfold within 3 min of addition of prolactin or phorbol 12-tetradecanoate 13-acetate. Rat prolactin stimulated PKC maximally at 10(-12) M, whereas ovine prolactin was maximally stimulat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Buckley AR,Crowe PD,Russell DH

更新日期：1988-11-01 00:00:00