Abstract:
:Normal mouse vaginal epithelial cells isolated from ovariectomized ca. 40-day-old BALB/cCrg1 mice were purified by Percoll density gradient centrifugation and grown in primary culture using a collagen gel matrix and a serum-free complete medium. During the 9-day culture period, a 6-fold increase in cell number was observed. Addition of estrogen to the medium did not enhance epithelial cell proliferation. In fact, all doses of estrogen (180 fM to 18 nM) were inhibitory, resulting in only a 3- to 4-fold increase in cell number by day 9 of culture. Continuous exposure to estradiol (1.8 nM) for 9 days in the serum-free complete medium resulted in a decrease in cytosolic estrogen receptors with associated nuclear accumulation of estrogen receptors. A corresponding increase of cytosolic progestin receptors was also observed, indicating that no qualitative modification of the estrogen receptor system had occurred. Thus, despite its effectiveness in specific product synthesis (progestin receptors), estrogen does not stimulate proliferation of vaginal cells in this culture system, but rather inhibits epithelial cell proliferation.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Uchima FD,Edery M,Iguchi T,Larson L,Bern HAdoi
10.1016/0304-3835(87)90124-8subject
Has Abstractpub_date
1987-06-01 00:00:00pages
227-35issue
3eissn
0304-3835issn
1872-7980pii
0304-3835(87)90124-8journal_volume
35pub_type
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