Identification and characterization of zinc finger encoding genes from the tumoral exocrine pancreatic cell line AR42J.

Abstract:

:Zinc finger transcription factors are DNA-binding proteins that are known to determine the identity of cells by regulating cell-specific gene expression. In addition, mutations in some members of this family have been found to be associated with several neoplasias, including Wilms' tumor and leukemias. Because the mechanisms that regulate normal, as well as neoplastic, pancreatic cell differentiation are poorly understood, we are searching for pancreas-enriched transcription factors that may determine the identity of pancreatic cells. Towards this end, we have used the polymerase chain reaction and degenerate primers against the highly conserved (Cys2-His2) zinc finger domain to amplify novel transcription factor encoding cDNAs from the well-characterized pancreatic acinar cell line AR42J. Using this approach, we have identified 17 different zinc finger encoding cDNAs (AZF-1 to -17). Sequence analysis shows that all of these clones encode for different zinc finger peptides which share the consensus DNA binding motif with the Drosophila transcription factor krüppel. As a first step in the characterization of these genes, the purified PCR products were used to determine their spatial pattern of expression by northern blot analysis. Using these techniques, we find that numerous zinc finger encoding genes are expressed in AR42J acinar cells as well as in normal adult rat pancreas and suggest that they may play a role as transcription factors in exocrine pancreatic cells.

journal_name

Cancer Lett

journal_title

Cancer letters

authors

Mesa K,Gebelein B,Cook T,Urrutia R

doi

10.1016/0304-3835(96)04204-8

subject

Has Abstract

pub_date

1996-06-05 00:00:00

pages

143-9

issue

2

eissn

0304-3835

issn

1872-7980

pii

0304-3835(96)04204-8

journal_volume

103

pub_type

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