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An improved CTC isolation scheme for pairing with downstream genomics: Demonstrating clinical utility in metastatic prostate, lung and pancreatic cancer.

Abstract：

:Improvements in technologies to yield purer circulating tumor cells (CTCs) will enable a broader range of clinical applications. We have previously demonstrated the use of a commercially available cell-adhesion matrix (CAM) assay to capture invasive CTCs (iCTCs). To improve the purity of the isolated iCTCs, here we used fluorescence-activated cell sorting (FACS) in combination with the CAM assay (CAM + FACS). Our results showed an increase of median purity from the CAM assay to CAM + FACS for the spiked-in cell lines and patient samples analyzed from three different metastatic cancer types: castration resistant prostate cancer (mCRPC), non-small cell lung cancer (mNSCLC) and pancreatic ductal adenocarcinoma cancer (mPDAC). Copy number profiles for spiked-in mCRPC cell line and mCRPC patient iCTCs were similar to expected mCRPC profiles and a matched biopsy. A somatic epidermal growth factor receptor (EGFR) mutation specific to mNSCLC was observed in the iCTCs recovered from EGFR(+) mNSCLC cell lines and patient samples. Next-generation sequencing (NGS) of spiked-in pancreatic cancer cell line and mPDAC patient iCTCs showed mPDAC common mutations. CAM + FACS iCTC enrichment enables multiple downstream genomic characterizations across different tumor types.

journal_name

Cancer Lett

journal_title

Cancer letters

authors

Premasekharan G,Gilbert E,Okimoto RA,Hamirani A,Lindquist KJ,Ngo VT,Roy R,Hough J,Edwards M,Paz R,Foye A,Sood R,Copren KA,Gubens M,Small EJ,Bivona TG,Collisson EA,Friedlander TW,Paris PL

doi

10.1016/j.canlet.2016.06.017

subject

Has Abstract

pub_date

2016-09-28 00:00:00

pages

144-52

issue

1

eissn

0304-3835

issn

1872-7980

pii

S0304-3835(16)30377-9

journal_volume

380

pub_type

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