当前位置： SCI文献检索 > DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY期刊下所有文献 > Characterization, expression and function analysis of the TLR3 gene in golden pompano (Trachinotus ovatus).

Characterization, expression and function analysis of the TLR3 gene in golden pompano (Trachinotus ovatus).

Abstract：

:Toll-like receptors (TLRs)are pattern recognition receptors (PRRs) that are important in invertebrate innate immunity for the recognition and elimination of pathogens. Although they were reported in many fishes, Toll-like receptors subfamily contain a large number of members with different functions that need to research in deep. In the present study, the full-length cDNA of TLR3 from the golden pompano, Trachinotus ovatus, was cloned and characterized. The full length of ToTLR3 cDNA was 3710 bp including an open reading frame of 2760 bp encoding a peptide of 919 amino acids. The derived amino acids sequence comprised of 14 leucine-rich repeats (LRR), capped with LRRCT followed by transmembrane domain and cytoplasmic Toll/IL-1R domain (TIR). Multiple sequence alignment and phylogenetic analysis revealed that ToTLR3 shared the highest similarity to the teleost fish and suggested ToTLR3 is fairly conservative in evolution process. Tissues distribution analysis indicated that ToTLR3 showed a tissue-specific variation with high expression in blood and liver. After the fish were stimulated by poly(I:C), flagellin and LPS, ToTLR3 expression in the liver, intestine, blood, kidney, skin and muscle was significantly upregulated in a time-depended manner, especially in immune related tissues such as liver, blood and kidney. Binding assay revealed the specificity of rToTLR3 for pathogen-associated molecular patterns (PAMPs) and bacteria that included Vibrio harveyi, V. vulnificus, V. anguillarum, Photobacterium damselae, Escherichia coli, Aeromonas hydrophila, Staphylococcus aureus and PolyI:C, LPS, Flagellin, and PGN. In addition, a luciferase reporter assay showed that overexpression ToTLR3 significantly increased NF-κB activity. Collectively, our results suggested that ToTLR3 might play an important role as a pattern recognition receptor (PRR) in the immune response towards pathogen infections, and transmiss the danger signal to downstream signaling pathways.

journal_name

Dev Comp Immunol

journal_title

Developmental and comparative immunology

authors

Wu M,Zhu KC,Guo HY,Guo L,Liu B,Jiang SG,Zhang DC

doi

10.1016/j.dci.2020.103977

subject

Has Abstract

pub_date

2021-04-01 00:00:00

pages

103977

eissn

0145-305X

issn

1879-0089

pii

S0145-305X(20)30531-0

journal_volume

117

pub_type

杂志文章

相关文献

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY文献大全
  • Identification and characterization of equine blood plasmacytoid dendritic cells.

    abstract：:Dendritic cells (DC) are antigen-presenting cells that can be classified into three major cell subsets: conventional DC1 (cDC1), cDC2 and plasmacytoid DCs (pDC), none of which have been identified in horses. Therefore, the objective of this study was to identify and characterize DC subsets in equine peripheral blood, ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2016.08.005

    authors： Ziegler A,Marti E,Summerfield A,Baumann A

    更新日期：2016-12-01 00:00:00

  • Microarray analyses reveal distinct roles for Rel proteins in the Drosophila immune response.

    abstract：:The NF-kappaB group of transcription factors play an important role in mediating immune responses in organisms as diverse as insects and mammals. The fruit fly Drosophila melanogaster express three closely related NF-kappaB-like transcription factors: Dorsal, Dif, and Relish. To study their roles in vivo, we used micr...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2007.04.001

    authors： Pal S,Wu J,Wu LP

    更新日期：2008-01-01 00:00:00

  • Identification of a novel antimicrobial peptide from the sea star Patiria pectinifera.

    abstract：:Antimicrobial peptides (AMPs) are components of innate immunity found in many forms of life. However, there have been no reports of AMPs in sea star (Phylum Echinodermata). Here we report the isolation and characterization of a novel antimicrobial peptide from the coelomic epithelium extract of the sea star Patiria pe...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.05.002

    authors： Kim CH,Go HJ,Oh HY,Park JB,Lee TK,Seo JK,Elphick MR,Park NG

    更新日期：2018-09-01 00:00:00

  • Macrophage activation differentially modulates particle binding, phagocytosis and downstream antimicrobial mechanisms.

    abstract：:Phagocytosis provides a critical first line of defense against invading pathogens. Engagement of particles through receptor-mediated binding precedes internalization and induction of cellular antimicrobial responses. Phagocytes have the capacity to differentially regulate binding and internalization processes through ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2010.06.006

    authors： Rieger AM,Hall BE,Barreda DR

    更新日期：2010-11-01 00:00:00

  • Relationship between circulating thyroid hormones and humoral immunity in immature male chickens.

    abstract：:The objective of this experiment was to examine the relationship between levels of circulating T3, T4, and humoral immunity in immature male chickens. Three week old Single Comb White Leghorn male chicks were used as the experimental animals. In order to produce a wide range of circulating thyroid hormone concentratio...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(86)90029-7

    authors： Bachman SE,Mashaly MM

    更新日期：1986-07-01 00:00:00

  • Pre-acute hepadnaviral infection is associated with activation-induced apoptotic death of lymphocytes in the woodchuck (Marmota monax) model of hepatitis B.

    abstract：:Woodchucks (Marmota monax) infected with woodchuck hepatitis virus (WHV) represent a highly valuable immunopathogenic model of hepatitis B virus (HBV) infection. Both WHV and HBV are noncytopathic hepadnaviruses which induce a strong but delayed virus-specific cellular immune response believed to be a cause of hepatit...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2010.05.001

    authors： Gujar SA,Jenkins AK,Macparland SA,Michalak TI

    更新日期：2010-09-01 00:00:00

  • An alternative internal splicing site defines new Ikaros isoforms in Pleurodeles waltl.

    abstract：:The Ikaros gene encodes a family of transcription factors which plays a crucial role in hematopoiesis. To improve our knowledge about the immune system of Pleurodeles waltl, we sequenced the cDNA coding for the Ik-1 isoform of that salamander and analyzed its tissue expression by semi-quantitative RT-PCR. Ikaros trans...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(02)00014-9

    authors： Boudarra N,Frippiat C,Dournon C,Frippiat JP

    更新日期：2002-09-01 00:00:00

  • The amphibian (Xenopus laevis) colony-stimulating factor-1 and interleukin-34-derived macrophages possess disparate pathogen recognition capacities.

    abstract：:Pathogens such as the Frog Virus 3 (FV3) ranavirus are contributing to the worldwide amphibian declines. While amphibian macrophages (Mϕs) are central to the immune defenses against these viruses, the pathogen recognition capacities of disparate amphibian Mϕ subsets remain unexplored. In turn, Mϕ differentiation and f...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2019.04.011

    authors： Yaparla A,Docter-Loeb H,Melnyk MLS,Batheja A,Grayfer L

    更新日期：2019-09-01 00:00:00

  • Characterization of immunoglobulin light chain utilization and variable family diversity in rainbow trout.

    abstract：:This study characterizes immunoglobulin light chain (IgL) expression and variable family usage in rainbow trout. IgL transcripts were generated by 5' RACE from both immune and TNP-KLH immunized fish. Phylogenetic analysis revealed that the IgL variable regions clustered into seven different families: three kappa famil...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2019.103566

    authors： Rego K,Bengtén E,Wilson M,Hansen JD,Bromage ES

    更新日期：2020-03-01 00:00:00

  • An azurocidin-like protein is induced in Trichoplusia ni larval gut cells after bacterial challenge.

    abstract：:Trichoplusia ni immune genes up-regulated in response to bacterial infection have been isolated using differential display polymerase chain reaction. Here we report the cloning and characterisation of a gut-specific immune gene encoding an azurocidin-like protein. The deduced protein is 317 amino acid residues long wi...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(02)00009-5

    authors： Kang D,Lundström A,Liu G,Steiner H

    更新日期：2002-07-01 00:00:00

  • The alternatively spliced porcine FcγRI regulated PRRSV-ADE infection and proinflammatory cytokine production.

    abstract：:Receptors for the Fc region of IgG (FcγRs) play a key role in protecting the immune system and host from infection. In this study, we described the cloning, sequencing and characterization of porcine FcγRI, and reported six different FcγRI isoforms, four of which have never been reported before. Further analysis revea...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.09.019

    authors： Shi P,Su Y,Li Y,Zhang L,Lu D,Li R,Zhang L,Huang J

    更新日期：2019-01-01 00:00:00

  • Teleost contributions to the understanding of mycobacterial diseases.

    abstract：:Few pathogens have shaped human medicine as the mycobacteria. From understanding biological phenomena driving disease spread, to mechanisms of host-pathogen interactions and antibiotic resistance, the Mycobacterium genus continues to challenge and offer insights into the basis of health and disease. Teleost fish model...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.dci.2019.02.011

    authors： Hodgkinson JW,Belosevic M,Elks PM,Barreda DR

    更新日期：2019-07-01 00:00:00

  • High doses of sodium ascorbate act as a prooxidant and protect gnotobiotic brine shrimp larvae (Artemia franciscana) against Vibrio harveyi infection coinciding with heat shock protein 70 activation.

    abstract：:Ascorbate is an essential nutrient commonly regarded as an antioxidant. In this study, using axenic brine shrimp and pathogenic strain Vibrio harveyi as the host-pathogen model, we confirmed that pretreatment of sodium ascorbate (NaAs), at an optimum concentration, was a prooxidant by generation of hydrogen peroxide, ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.11.007

    authors： Han B,Kaur VI,Baruah K,Nguyen VD,Bossier P

    更新日期：2019-03-01 00:00:00

  • Mitigation of ER-stress and inflammation by chemokine (C-C motif) ligand 21 during early pregnancy.

    abstract：:The immune system plays an important role in pregnancy. Chemokines recruit leukocytes at the maternal-fetal interface during early pregnancy. However, the role of the chemokine, C-C motif chemokine ligand 21 (CCL21), is less known. The aim of this study was to identify the expression of CCL21 and its receptor, CCR7, i...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2019.01.016

    authors： Bae H,Lim W,Bazer FW,Whang KY,Song G

    更新日期：2019-05-01 00:00:00

  • Chemotaxis of nurse shark leukocytes.

    abstract：:Studies were conducted to determine the ability of leukocytes from the nurse shark to migrate in an in vitro micropore filter chemotaxis assay and to determine optimal assay conditions and suitable attractants for such an assay. A migratory response was seen with several attractants: activated rat serum, activated sha...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(85)90113-2

    authors： Obenauf SD,Smith SH

    更新日期：1985-04-01 00:00:00

  • Influence of breast milk polyamines on suckling rat immune system maturation.

    abstract：:The aim of this study was to ascertain whether the supplementation of polyamines present in breast milk, i.e. spermine (SPM) and spermidine (SPD), influenced the post-natal maturation of the systemic and intestinal immune system in rats. From birth, pups daily received SPM or SPD. At 5, 11 and 18 days old, small intes...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2009.10.001

    authors： Pérez-Cano FJ,González-Castro A,Castellote C,Franch A,Castell M

    更新日期：2010-02-01 00:00:00

  • Extracellular vesicles from cod (Gadus morhua L.) mucus contain innate immune factors and deiminated protein cargo.

    abstract：:Extracellular vesicles are released from cells and participate in cell communication via transfer of protein and genetic cargo derived from the parent cells. EVs play roles in normal physiology and immunity and are also linked to various pathological processes. Peptidylarginine deiminases (PADs) are phylogenetically c...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2019.103397

    authors： Magnadóttir B,Kraev I,Guðmundsdóttir S,Dodds AW,Lange S

    更新日期：2019-10-01 00:00:00

  • The Drosophila IMD pathway in the activation of the humoral immune response.

    abstract：:The IMD pathway signaling plays a pivotal role in the Drosophila defense against bacteria. During the last two decades, significant progress has been made in identifying the components and deciphering the molecular mechanisms underlying this pathway, including the means of bacterial sensing and signal transduction. Wh...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.dci.2013.05.014

    authors： Kleino A,Silverman N

    更新日期：2014-01-01 00:00:00

  • Penaeus vannamei serine proteinase inhibitor 7 (LvSerpin7) acts as an immune brake by regulating the proPO system in AHPND-affected shrimp.

    abstract：:Acute hepatopancreatic necrosis disease (AHPND) is a recently emerged disease in aqua cultured shrimp that is caused by virulent strains of Vibrio parahaemolyticus (VP). Our previous study used transcriptomics to identify key pathogenic factors in the stomach of AHPND-infected shrimp (Litopenaeus vannamei), and here w...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2019.103600

    authors： Apitanyasai K,Chang CC,Ng TH,Ng YS,Liou JH,Lo CF,Lin SS,Wang HC

    更新日期：2020-05-01 00:00:00

  • Combinational recognition of bacterial lipoproteins and peptidoglycan by chicken Toll-like receptor 2 subfamily.

    abstract：:Human Toll-like receptor 2 (TLR2) subfamily recognizes bacterial lipoproteins (BLP) and peptidoglycan (PGN). According to the genome information, chicken has structural orthologs of TLRs1 and 2, in addition to TLRs3, 4, 5 and 7. Chicken has two additional TLRs, TLR15 and TLR21, whose orthologs human lacks. The chicken...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2007.05.003

    authors： Higuchi M,Matsuo A,Shingai M,Shida K,Ishii A,Funami K,Suzuki Y,Oshiumi H,Matsumoto M,Seya T

    更新日期：2008-01-01 00:00:00

  • Establishment and characterization of two head kidney macrophage cell lines from large yellow croaker (Larimichthys crocea).

    abstract：:Two continuous macrophage cell lines (LCM07 and LCM10) were established for the first time from the head kidney of the marine fish large yellow croaker (Larimichthys crocea). To date, both cell lines have been subcultured for more than 100 passages in 12 months. Notably, the LCM07 and LCM10 cells have distinct morphol...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2019.103477

    authors： Cui K,Li Q,Xu D,Zhang J,Gao S,Xu W,Mai K,Ai Q

    更新日期：2020-01-01 00:00:00

  • Evolution of low molecular weight immunoglobulins. V. Degree of antigenic relationship between the 7S immunoglobulins of mammals, birds, and lower vertebrates to the turkey IgY.

    abstract：:There are close antigenic relations between the turkey IgY--the dominant 7S Ig of serum--and the corresponding IgY-like Igs of chicken, duck, goose, tortoise, and frog as well as serum or colostral IgA of man and swine. The RIA-systems used for the determination of immunological cross-reactivity among the Ig types con...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(86)90027-3

    authors： Hädge D,Ambrosius H

    更新日期：1986-07-01 00:00:00

  • The upregulation of immune responses in tyrosine hydroxylase (TH) silenced Litopenaeus vannamei.

    abstract：:Catecholamines (CAs) play a crucial role in maintaining physiological and immune homeostasis in invertebrates and vertebrates under stressful conditions. Tyrosine hydroxylase (TH) is the first and rate-limiting enzyme in CA synthesis. To develop an effective CA-related immunological defense system against stress and p...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2016.11.002

    authors： Mapanao R,Chang CC,Cheng W

    更新日期：2017-02-01 00:00:00

  • Effect of experimental microbial challenge on the expression of defense molecules in Eisenia foetida earthworm.

    abstract：:Earthworms are able to protect themselves against invading pathogens due to efficient innate defense mechanisms. Currently, two types of antimicrobial factors including lysozyme-like molecule and factors with hemolytic activity, as well as a pattern recognition protein named coelomic cytolytic factor (CCF) have been i...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2004.01.001

    authors： Köhlerová P,Beschin A,Silerová M,De Baetselier P,Bilej M

    更新日期：2004-06-01 00:00:00

  • Phylogeny of lymphocyte heterogeneity. I. Membrane immunoglobulins of teleost lymphocytes.

    abstract：:Immunofluorescence studies of bream lymphoid tissues revealed that our 90% of the lymphocytes from blood, anterior kidney, spleen and thymus exhibited membrane immunoglobulin determinants. Furthermore, a majority of such cells were observed to undergo patching and capping when the membrane proteins were complexed with...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(77)80004-9

    authors： Clem LW,McLean WE,Shankey VT,Cuchens MA

    更新日期：1977-04-01 00:00:00

  • Goldfish (Carassius auratus L.) as a model system to study the growth factors, receptors and transcription factors that govern myelopoiesis in fish.

    abstract：:The process of myeloid cell development (myelopoiesis) in fish has mainly been studied in three cyprinid species: zebrafish (Danio rerio), ginbuna carp (Carassius auratus langsdorfii) and goldfish (C. auratus, L.). Our studies on goldfish myelopoiesis have utilized in vitro generated primary kidney macrophage (PKM) cu...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.dci.2015.10.024

    authors： Katzenback BA,Katakura F,Belosevic M

    更新日期：2016-05-01 00:00:00

  • Correlated expression profile of extracellular matrix-related molecules during the inflammatory response of the teleost fish gilthead seabream.

    abstract：:Extracellular matrix (ECM) components, in addition to their structural functions, interact with cell surface receptors and intracellular components to modulate the transduction of signals for cell growth, differentiation, migration, proliferation, polarization, apoptosis and inflammation. Our previous findings in the ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2010.05.007

    authors： Castillo-Briceño P,Arizcun-Arizcun M,Meseguer J,Mulero V,García-Ayala A

    更新日期：2010-10-01 00:00:00

  • Molecular characterization and function of a PTEN gene from Litopenaeus vannamei after Vibrio alginolyticus challenge.

    abstract：:PTEN, a tumor suppressor gene, suppresses cell survival, growth, apoptosis, cell migration and DNA damage repair by inhibiting the PI3K/AKT signaling pathway. In this study, the full-length Litopenaeus vannamei PTEN (LvPTEN) cDNA was obtained, containing a 5'UTR of 59bp, an ORF of 1269bp and a 3'UTR of 146bp besides t...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2016.01.004

    authors： Xie CY,Kong JR,Zhao CS,Xiao YC,Peng T,Liu Y,Wang WN

    更新日期：2016-06-01 00:00:00

  • A novel junctional adhesion molecule A (CgJAM-A-L) from oyster (Crassostrea gigas) functions as pattern recognition receptor and opsonin.

    abstract：:Junctional adhesion molecule (JAM), a subfamily of immunoglobulin superfamily (IgSF) with a couple of immunoglobulin domains, can act as regulator in homeostasis and inflammation of vertebrates. In the present study, a structural homolog of JAM-A (designated CgJAM-A-L) was screened out from oyster, Crassostrea gigas, ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2015.09.011

    authors： Liu C,Wang M,Jiang S,Wang L,Chen H,Liu Z,Qiu L,Song L

    更新日期：2016-02-01 00:00:00

  • 2E3, a new marker that selectively identifies porcine CD4+ naive T cells.

    abstract：:We describe a novel antigen recognized by mAb 2E3 selectively expressed in the periphery by a subset of porcine CD4+ T cells. Both, CD4+CD8alpha- and CD4+CD8alphalow T cell subpopulations express this antigen. CD4+2E3+ T cells show phenotypical and functional characteristics of nai;ve cells. The majority of them are C...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(03)00137-x

    authors： Revilla C,Rodríguez-Carreño MP,Alvarez B,Chamorro S,Alonso LM,Ezquerra A,Alonso F,Domínguez J

    更新日期：2004-03-01 00:00:00