Abstract:
:We describe a novel antigen recognized by mAb 2E3 selectively expressed in the periphery by a subset of porcine CD4+ T cells. Both, CD4+CD8alpha- and CD4+CD8alphalow T cell subpopulations express this antigen. CD4+2E3+ T cells show phenotypical and functional characteristics of nai;ve cells. The majority of them are CD29low, CD45RAhigh, CD49dlow, CD11alow, CD18low, and SLA-II-. After mitogen activation CD4+2E3+ T cells express high levels of IL-2 mRNA, but only traces of IFN-gamma or IL-4 mRNA. Indeed a minor percentage of cells stained positive for IFN-gamma when assessed by flow cytometry. Moreover, CD4+2E3+ T cells did not proliferate in response to the recall antigen lysozyme, although they did efficiently to the mitogen ConA. By contrast, CD4+2E3- T cells show phenotypical and functional characteristics of primed cells. They express markers associated to a memory phenotype, respond to the recall antigen lysozyme, and produce high amounts of IFN-gamma and IL-4.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Revilla C,Rodríguez-Carreño MP,Alvarez B,Chamorro S,Alonso LM,Ezquerra A,Alonso F,Domínguez Jdoi
10.1016/s0145-305x(03)00137-xkeywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
239-50issue
3eissn
0145-305Xissn
1879-0089pii
S0145305X0300137Xjournal_volume
28pub_type
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