Abstract：
:Trichoplusia ni immune genes up-regulated in response to bacterial infection have been isolated using differential display polymerase chain reaction. Here we report the cloning and characterisation of a gut-specific immune gene encoding an azurocidin-like protein. The deduced protein is 317 amino acid residues long with a hydrophobic C-terminus and a predicted 17-residue signal peptide. The mature T. ni protein shows 30% identity to human azurocidin, an antibacterial protein. Like azurocidin, the T. ni protein contains two amino acid substitutions in the active site triad normally present in serine proteases. The T. ni protein was synthesised with a six-histidine C-terminal extension using the baculovirus expression system. Sequencing of the recombinant azurocidin-like protein confirmed the predicted cleavage of the signal peptide. Northern blots show that T. ni azurocidin-like protein is expressed solely in the larval gut and that expression is up-regulated by injecting or feeding bacteria. Expression reaches its highest level at 10 h after bacteria injection.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Kang D,Lundström A,Liu G,Steiner Hdoi
10.1016/s0145-305x(02)00009-5keywords:
subject
Has Abstractpub_date
2002-07-01 00:00:00pages
495-503issue
6eissn
0145-305Xissn
1879-0089pii
S0145305X02000095journal_volume
26pub_type
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