An azurocidin-like protein is induced in Trichoplusia ni larval gut cells after bacterial challenge.


:Trichoplusia ni immune genes up-regulated in response to bacterial infection have been isolated using differential display polymerase chain reaction. Here we report the cloning and characterisation of a gut-specific immune gene encoding an azurocidin-like protein. The deduced protein is 317 amino acid residues long with a hydrophobic C-terminus and a predicted 17-residue signal peptide. The mature T. ni protein shows 30% identity to human azurocidin, an antibacterial protein. Like azurocidin, the T. ni protein contains two amino acid substitutions in the active site triad normally present in serine proteases. The T. ni protein was synthesised with a six-histidine C-terminal extension using the baculovirus expression system. Sequencing of the recombinant azurocidin-like protein confirmed the predicted cleavage of the signal peptide. Northern blots show that T. ni azurocidin-like protein is expressed solely in the larval gut and that expression is up-regulated by injecting or feeding bacteria. Expression reaches its highest level at 10 h after bacteria injection.


Dev Comp Immunol


Kang D,Lundström A,Liu G,Steiner H





Has Abstract


2002-07-01 00:00:00














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    abstract::Levels of interferon (IFN)-γ and interleukin (IL)-10 were measured in the serum of immunocompetent and immunosuppressed New Zealand White rabbits naturally infected with Encephalitozoon cuniculi. IFN-γ levels were elevated in infected rabbits, and a synergic effect was observed in animals treated with the immunosuppre...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Rodríguez-Tovar LE,Castillo-Velázquez U,Arce-Mendoza AY,Nevárez-Garza AM,Zarate-Ramos JJ,Hernández-Vidal G,Rodríguez-Ramírez HG,Trejo-Chávez A

    更新日期:2016-09-01 00:00:00

  • WSV399, a viral tegument protein, interacts with the shrimp protein PmVRP15 to facilitate viral trafficking and assembly.

    abstract::Viral responsive protein 15 (PmVRP15) has been identified as a highly up-regulated gene in the hemocyte of white spot syndrome virus (WSSV)-infected shrimp Penaeus monodon. However, the function of PmVRP15 in host-viral interaction was still unclear. To elucidate PmVRP15 function, the interacting partner of PmVRP15 fr...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Jaree P,Senapin S,Hirono I,Lo CF,Tassanakajon A,Somboonwiwat K

    更新日期:2016-06-01 00:00:00

  • The novel six LIM and one PET domain-containing protein Lmpt is involved in the immune response through activation of the NF-κB signalling pathway in the crustacean, Macrobrachium nipponense.

    abstract::The four-and-a-half LIM-only protein family of transcription co-factors participates in various cellular processes, such as cell proliferation, cell differentiation, apoptosis, cell adhesion, migration, transcription and signal transduction. However, the knowledge of the structural characteristics and immune functions...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Xu L,Zhou X,Wang P,Jin Q,Zhu T,Chen M,Xu H

    更新日期:2021-03-01 00:00:00

  • Postnatal changes in epigenetic modifications of neutrophils of foals are associated with increased ROS function and regulation of neutrophil function.

    abstract::Neonates of all species, including foals, are highly susceptible to infection, and neutrophils play a crucial role in innate immunity to infection. Evidence exists that neutrophils of neonatal foals are functionally deficient during the first weeks of life, including expression of cytokine genes such as IFNG. We hypot...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Dindot SV,Doan RN,Kuskie KR,Hillman PR,Whitfield CM,McQueen CM,Bordin AI,Bourquin JR,Cohen ND

    更新日期:2018-10-01 00:00:00

  • Characterization and role of suppressor of cytokine signaling 1a (SOCS1a) in a teleost fish, Miichthys miiuy.

    abstract::The suppressor of cytokine signaling 1 (SOCS1) is a crucial regulator in the immune systems of mammals, which functions classically as a negatively regulator in the IFN signaling pathways. However, data on functional characterization of SOCS1 in lower vertebrates is limited. In this study, we identified and characteri...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Zhao X,Huo R,Song W,Xu T

    更新日期:2018-01-01 00:00:00

  • Encapsulation ability of Drosophila melanogaster: a genetic analysis.

    abstract::Insects are able to effectively recognize parasitoid eggs or larvae and to eliminate them by formation of a hemocytic capsule. Although the cellular process is now well documented, the genetic aspects of recognition of foreignness and the encapsulation process are still poorly understood. Experiments using the isofema...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Carton Y,Boulétreau M

    更新日期:1985-04-01 00:00:00

  • Isolation and characterization of earthworm hemolysins and agglutinins.

    abstract::Hemolytic activity in coelomic fluid of Eisenia fetida (ECF) is due to three proteins H1, H2, H3 with molecular weights of 46, 43 and 40 kD, respectively. These proteins were isolated by preparative PAGE. H1 and H2 were shown to be stable in SDS and alpha-2-ME whereas H3 splits into two fragments with molecular weight...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Eue I,Kauschke E,Mohrig W,Cooper EL

    更新日期:1998-01-01 00:00:00

  • Functional elucidation of LvToll 3 receptor from P. vannamei through RNA interference and its potential role in the shrimp antiviral response.

    abstract::There is a continuing debate on whether an antiviral immunity similar to vertebrate interferon response exists in invertebrates. Recent advances in penaeid immunology identified several new members of the Toll receptor family and one of these is LvToll3 (Litopenaeus vannamei Toll3). It is hypothesized in this study th...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Guanzon DAV,Maningas MBB

    更新日期:2018-07-01 00:00:00

  • Demonstration of T cell and macrophage progenitors in carp (Cyprinus carpio) kidney hematopoietic tissues. Development of clonal assay system for carp hematopoietic cells.

    abstract::Single hematopoietic cells from carp (Cyprinus carpio) kidney were seeded to each well of 96-well plates and cultured in the presence of a supporting cell layer and conditioned media (CM). The CM were obtained from bulk-cultured carp hematopoietic cells, in which T and macrophage-lineage cells rapidly proliferated as ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Katakura F,Yamaguchi T,Yoshida M,Moritomo T,Nakanishi T

    更新日期:2010-06-01 00:00:00

  • Exploring the relationships between amphibian (Xenopus laevis) myeloid cell subsets.

    abstract::The differentiation of distinct leukocyte subsets is governed by lineage-specific growth factors that elicit disparate expression of transcription factors and markers by the developing cell populations. For example, macrophages (Mφs) and granulocytes (Grns) arise from common granulocyte-macrophage progenitors in respo...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Yaparla A,Koubourli DV,Popovic M,Grayfer L

    更新日期:2020-12-01 00:00:00

  • Molecular cloning, characterization and function of a germinal center kinase MST4 gene from Litopenaeus vannamei in response to Vibrio alginolyticus challenge in TLR-TRAF6 signaling pathway.

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    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Zhao CS,Huang D,Peng T,Huang MZ,Xie CY,Chen J,Kong JR,Xie RC,Liu Y,Wang WN

    更新日期:2017-08-01 00:00:00

  • Arasin 1, a proline-arginine-rich antimicrobial peptide isolated from the spider crab, Hyas araneus.

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    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Stensvåg K,Haug T,Sperstad SV,Rekdal O,Indrevoll B,Styrvold OB

    更新日期:2008-01-01 00:00:00

  • Siglecs: A journey through the evolution of sialic acid-binding immunoglobulin-type lectins.

    abstract::Siglecs (sialic acid-binding immunoglobulin-type lectins) are a family of immune regulatory receptors predominantly found on the cells of the hematopoietic system. A V-set Ig-like domain mediates the recognition of different sialylated glycoconjugates, which can lead to the activation or inhibition of the immune respo...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审


    authors: Bornhöfft KF,Goldammer T,Rebl A,Galuska SP

    更新日期:2018-09-01 00:00:00

  • IRF11 regulates positively type I IFN transcription and antiviral response in mandarin fish, Siniperca chuatsi.

    abstract::In vertebrates, a total of eleven interferon (IFN) regulatory factors (IRFs), IRF1 to IRF11 are reported, with the conserved presence of IRF1 to IRF9 in all classes of vertebrates. However, IRF10 has been reported only in fish and birds, and IRF11 seems to be a fish specific IRF member. In this study, IRF11 in mandari...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Li L,Chen SN,Nie P

    更新日期:2021-01-01 00:00:00

  • A shrimp pacifastin light chain-like inhibitor: molecular identification and role in the control of the prophenoloxidase system.

    abstract::Pacifastin is a recently classified family of serine proteinase inhibitors that play essential roles in various biological processes, including in the regulation of the melanization cascade. Here, a novel pacifastin-related gene, termed PmPacifastin-like, was identified from a reverse suppression subtractive hybridiza...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Sangsuriya P,Charoensapsri W,Chomwong S,Senapin S,Tassanakajon A,Amparyup P

    更新日期:2016-01-01 00:00:00

  • Characterization of serum immunoglobulins in a chondrostean fish, Acipenser baeri.

    abstract::The euglobulin fraction of sturgeon (Acipenser baeri) serum was analyzed using electrophoretic and immunoblotting techniques. The major protein of this fraction is an IgM-like molecule composed of equimolar 70-kDa glycosylated H chains and 26-30 kDa L chains. In the absence of a reducing agent, the L and H polypeptide...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Partula S,Charlemagne J

    更新日期:1993-11-01 00:00:00

  • Chicken interferons, their receptors and interferon-stimulated genes.

    abstract::The prevalence of pathogenic viruses is a serious issue as they pose a constant threat to both the poultry industry and to human health. To prevent these viral infections an understanding of the host-virus response is critical, especially for the development of novel therapeutics. One approach in the control of viral ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审


    authors: Goossens KE,Ward AC,Lowenthal JW,Bean AG

    更新日期:2013-11-01 00:00:00

  • Hemolin-A lepidopteran anti-viral defense factor?

    abstract::Immunity in insects has largely focused on responses towards bacteria and fungi, but recently the study of immune responses against viral infections has also received attention. In Lepidoptera, phagocytosis and encapsulation mediated by hemocytes, and apoptosis are part of the response against virus infection; however...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审


    authors: Terenius O

    更新日期:2008-01-01 00:00:00

  • Preliminary characterization of hemolymph coagulation in Anopheles gambiae larvae.

    abstract::Hemolymph coagulation is a first response to injury, impeding infection, and ending bleeding. Little is known about its molecular basis in insects, but clotting factors have been identified in the fruit fly Drosophila melanogaster. Here, we have begun to study coagulation in the aquatic larvae of the malaria vector mo...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Agianian B,Lesch C,Loseva O,Dushay MS

    更新日期:2007-01-01 00:00:00

  • The Toll immune signaling pathway control conserved anti-dengue defenses across diverse Ae. aegypti strains and against multiple dengue virus serotypes.

    abstract::Dengue virus has become one of the most important arboviral pathogens affecting the world today. The virus is transmitted among humans by the mosquitoes Aedes aegypti and Ae. albopictus. Like other vector-borne pathogens, this virus encounters innate immune defenses within the mosquito vector that limit infection. We ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Ramirez JL,Dimopoulos G

    更新日期:2010-06-01 00:00:00

  • 2E3, a new marker that selectively identifies porcine CD4+ naive T cells.

    abstract::We describe a novel antigen recognized by mAb 2E3 selectively expressed in the periphery by a subset of porcine CD4+ T cells. Both, CD4+CD8alpha- and CD4+CD8alphalow T cell subpopulations express this antigen. CD4+2E3+ T cells show phenotypical and functional characteristics of nai;ve cells. The majority of them are C...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Revilla C,Rodríguez-Carreño MP,Alvarez B,Chamorro S,Alonso LM,Ezquerra A,Alonso F,Domínguez J

    更新日期:2004-03-01 00:00:00

  • Immunorecognition in the freshwater bivalve, Corbicula fluminea. II. Isolation and characterization of a plasma opsonin with hemagglutinating activity.

    abstract::Opsonizing and agglutinating activities of plasma from the freshwater clam, Corbicula fluminea, were found to be inhibited by the sugars, 2-deoxy-D-glucose (deoxy-Glu) and N-acetyl-D-galactosamine (GalNAc). The plasma opsonin/agglutinin was subsequently isolated by a two-step separation procedure. Aldehyde-fixed rabbi...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Yang R,Yoshino TP

    更新日期:1990-10-01 00:00:00

  • Antimicrobial mechanisms of fish leukocytes.

    abstract::Early activation and coordination of innate defenses are critical for effective responses against infiltrating pathogens. Rapid engagement of immune cells provides a critical first line of defense soon after pathogen infiltration. Activation leads to a well-orchestrated set of events that sees the induction and regula...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审


    authors: Rieger AM,Barreda DR

    更新日期:2011-12-01 00:00:00

  • Differential expression and functional diversification of diverse immunoglobulin domain-containing protein (DICP) family in three gynogenetic clones of gibel carp.

    abstract::Diverse immunoglobulin (Ig) domain-containing protein (DICP) family is a novel bony fish-specific multi-gene family encoding diversified immune receptors. However, their function and the implication of binding partners remain unknown. In this study, we first identified 28 DICPs from three gibel carp gynogenetic clones...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Gao FX,Lu WJ,Wang Y,Zhang QY,Zhang YB,Mou CY,Li Z,Zhang XJ,Liu CW,Zhou L,Gui JF

    更新日期:2018-07-01 00:00:00

  • Lipopolysaccharide- and β-1,3-glucan-binding protein from Fenneropenaeus merguiensis functions as a pattern recognition receptor with a broad specificity for diverse pathogens in the defense against microorganisms.

    abstract::In crustaceans, lipopolysaccharide- and β-1,3-glucan-binding protein (LGBP) plays an important role in innate immunity by mediating the recognition of pathogens to host cells. Hereby, LGBP was cloned from Fenneropenaeus merguiensis hepatopancreas. Its full-length cDNA (1280 bp) had an open reading frame of 1101 bp, en...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Chaosomboon A,Phupet B,Rattanaporn O,Runsaeng P,Utarabhand P

    更新日期:2017-02-01 00:00:00

  • Atlantic salmon IPS-1 mediates induction of IFNa1 and activation of NF-kappaB and localizes to mitochondria.

    abstract::The striking difference in evolution of type I IFN genes of fish and mammals poses the question of whether these genes are induced through similar or different signalling pathways in the two vertebrate groups. Previous work has shown that expression of both Atlantic salmon (Salmo salar) IFNa1 and mammalian IFN-beta ge...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Lauksund S,Svingerud T,Bergan V,Robertsen B

    更新日期:2009-11-01 00:00:00

  • Identification and comparative analysis of three novel C-type lectins from the silkworm with functional implications in pathogen recognition.

    abstract::C-type lectins can act as pattern recognition receptors (PRRs) in innate immunity. Previously, we identified two C-type lectins from silkworm (Bombyx mori), BmLBP and BmMBP, as PRRs. In the present study, we identified three homologs of these lectins by searching the silkworm genome database. These novel B. mori low-e...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Takase H,Watanabe A,Yoshizawa Y,Kitami M,Sato R

    更新日期:2009-06-01 00:00:00

  • Heterogeneous nuclear ribonucleoprotein hrp36 acts as an alternative splicing repressor in Litopenaeus vannamei Dscam.

    abstract::Heterogeneous nuclear ribonucleoproteins (hnRNPs) are highly conserved from nematode to mammal because they play an important role in several aspects of pre-mRNA maturation, including RNA packaging and alternative splicing. In Drosophila, the hnRNP A1 homolog hrp36 regulates alternative splicing in several genes, incl...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Lee CW,Chen IT,Chou PH,Hung HY,Wang KV

    更新日期:2012-01-01 00:00:00

  • Bovine WC1(-) gammadeltaT cells incubated with IL-15 express the natural cytotoxicity receptor CD335 (NKp46) and produce IFN-gamma in response to exogenous IL-12 and IL-18.

    abstract::The gammadeltaT cells of ruminants are believed to participate in innate immunity and have been described with regulatory, inflammatory and cytotoxic functions. Here we describe a subset of CD3(+) TcR1(+) WC1(-)gammadeltaT cells expressing CD335 (NKp46), classically associated with CD3(-) natural killer (NK) cells, as...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Johnson WC,Bastos RG,Davis WC,Goff WL

    更新日期:2008-01-01 00:00:00

  • B-cell development in bovine fetuses proceeds via a pre-B like cell in bone marrow and lymph nodes.

    abstract::The production of B cells and the primary antibody repertoire in mammalian species other than rodents or man appears to depend on gut-associated lymphoid tissue. Bovine B cells are generated in ileal Peyer's patch from late gestational to juvenile age. However, little is known about where and when the bona fide B lymp...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Ekman A,Pessa-Morikawa T,Liljavirta J,Niku M,Iivanainen A

    更新日期:2010-08-01 00:00:00