Abstract:
:Nucleotide excision repair (NER) stands out among other DNA repair systems for its ability to process a diverse set of unrelated DNA lesions. In bacteria, NER damage detection is orchestrated by the UvrA and UvrB proteins, which form the UvrA2-UvrB2 (UvrAB) damage sensing complex. The highly versatile damage recognition is accomplished in two ATP-dependent steps. In the first step, the UvrAB complex samples the DNA in search of lesion. Subsequently, the presence of DNA damage is verified within the UvrB-DNA complex after UvrA has dissociated. Although the mechanism of bacterial NER damage detection has been extensively investigated, the role of ATP binding and hydrolysis by UvrA and UvrB during this process remains incompletely understood. Here, we report a pre-steady state kinetics Förster resonance energy transfer (FRET) study of the real-time interaction between UvrA, UvrB, and damaged DNA during lesion detection. By using UvrA and UvrB mutants harboring site-specific mutations in the ATP binding sites, we show for the first time that the dissociation of UvrA from the UvrAB-DNA complex does not require ATP hydrolysis by UvrB. We find that ATP hydrolysis by UvrA is not essential, but somehow facilitates the formation of UvrB-DNA complex, with ATP hydrolysis at the proximal site of UvrA playing a more critical role. Consistent with previous reports, our results indicated that the ATPase activity of UvrB is essential for the formation of UvrB-DNA complex but is not required for the binding of the UvrAB complex to DNA.
journal_name
DNA Repair (Amst)journal_title
DNA repairauthors
Kraithong T,Sucharitakul J,Buranachai C,Jeruzalmi D,Chaiyen P,Pakotiprapha Ddoi
10.1016/j.dnarep.2020.103024subject
Has Abstractpub_date
2021-01-01 00:00:00pages
103024eissn
1568-7864issn
1568-7856pii
S1568-7864(20)30284-6journal_volume
97pub_type
杂志文章相关文献
DNA REPAIR文献大全abstract::Methylation of the O(6)-methylguanine-DNA-methyltransferase (MGMT) promoter is associated with G:C to A:T transitions in the p53 gene in various human cancers, including lung cancer. In tumors with p53 mutation, MGMT promoter methylation is more common in advanced tumors than in early tumors. However, in tumors with w...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.04.016
更新日期:2008-08-02 00:00:00
abstract::Transcription factor II H (TFIIH) is composed of core TFIIH and Cdk-activating kinase (CAK) complexes. Besides transcription, TFIIH also participates in nucleotide excision repair (NER), verifying DNA lesions through its helicase components XPB and XPD. The assembly state of TFIIH is known to be affected by truncation...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2012.09.003
更新日期:2012-12-01 00:00:00
abstract::The RecQ family of DNA helicases performs essential functions in the maintenance of genomic stability in all organisms. In Deinococcus radiodurans, DR1289 is a special member of RecQ family with unique arrangement of three tandem HRDC domains in the C-terminus. A dr1289 mutant is hypersensitive to gamma-irradiation, U...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2006.09.006
更新日期:2007-02-04 00:00:00
abstract::The nonhomologous DNA end-joining pathway (NHEJ), a major pathway for repairing DNA double-strand breaks (DSBs), is essential for maintaining genomic stability. Knockout animals for components in this pathway demonstrate a distinct pattern of cell death in the developing brain. Here we demonstrate that cell death is a...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2003.08.011
更新日期:2003-12-09 00:00:00
abstract::DNA fiber fluorography is widely employed to study the kinetics of DNA replication, but the usefulness of this approach has been limited by the lack of freely-available automated analysis tools. Quantification of DNA fibers usually relies on manual examination of immunofluorescence microscopy images, which is laboriou...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2019.01.003
更新日期:2019-02-01 00:00:00
abstract::The detection of γ-H2AX focus is one of the most sensitive ways to monitor DNA double-strand breaks (DSBs). Although changes in γ-H2AX activity have been studied in tumor cells in colorectal cancer (CRC), changes in peripheral blood lymphocytes (PBLs) have not been examined previously. We hypothesize that higher level...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2017.03.001
更新日期:2017-05-01 00:00:00
abstract::The XPB DNA helicase, a subunit of the basal transcription factor TFIIH, is also involved in nucleotide excision repair (NER). We examined recruitment of NER proteins in XP-B cells from patients with mild or severe xeroderma pigmentosum (XP) having different XPB mutations using local UV-irradiation through filters wit...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2007.03.025
更新日期:2007-09-01 00:00:00
abstract::Eukaryotic cells repair ultraviolet light (UV)- and chemical carcinogen-induced DNA strand-distorting damage through the nucleotide excision repair (NER) pathway. Concurrent activation of the DNA damage checkpoints is also required to arrest the cell cycle and allow time for NER action. Recent studies uncovered critic...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2009.01.011
更新日期:2009-04-05 00:00:00
abstract::The Tousled-like kinases are involved in chromatin assembly, DNA repair, transcription, and chromosome segregation. In this work, we show that overexpression of TLK1B hastens repair of double strand breaks (DSBs) in mouse cells. We have identified Rad9 as a protein interacting tightly with TLK1B. TLK1B phosphorylates ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.09.005
更新日期:2009-01-01 00:00:00
abstract::The RecQ family of helicases are traditionally viewed as recombination factors, important for maintaining genome stability. RECQL5 is unique among these proteins in being associated with RNA polymerase II, the enzyme responsible for transcribing all protein-encoding genes in eukaryotes. Here, we describe the possible ...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2009.12.008
更新日期:2010-03-02 00:00:00
abstract::Several types of DNA lesion are induced after ionizing irradiation (IR) of which double strand breaks (DSBs) are expected to be the most lethal, although single strand breaks (SSBs) and DNA base damages are quantitatively in the majority. Proteins of the base excision repair (BER) pathway repair these numerous lesions...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.11.008
更新日期:2009-03-01 00:00:00
abstract::Mice defective in the mismatch repair (MMR) gene Msh2 manifest an enhanced predisposition to skin cancer associated with exposure to UVB radiation. This predisposition is further heightened if the mice are additionally defective for the nucleotide excision repair gene Xpc. To test the hypothesis that the predispositio...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(02)00143-x
更新日期:2002-11-03 00:00:00
abstract::Flaws in the DNA replication process have emerged as a leading driver of genome instability in human diseases. Alteration to replication fork progression is a defining feature of replication stress and the consequent failure to maintain fork integrity and complete genome duplication within a single round of S-phase co...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2018.08.017
更新日期:2018-11-01 00:00:00
abstract::The proximity of the mitochondrial genome to the respiratory chain, a major source of ROS (radical oxygen species), makes mtDNA more vulnerable to oxidative damage than nuclear DNA. Mitochondrial BER (base excision repair) is generally considered to be the main pathway involved in the prevention of oxidative lesion-in...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.11.004
更新日期:2009-03-01 00:00:00
abstract::Huntington disease (HD) is associated with an unstable trinucleotide CAG.CTG repeat expansion. Although the repeat length is inversely correlated with the age-at-onset of symptoms, variability between patients who have inherited the same HD repeat length clearly suggests that other factors influence this aspect of the...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2007.01.002
更新日期:2007-06-01 00:00:00
abstract::Both UVB radiation and DNA-breaking agents were previously reported to kill Arabidopsis stem cells. We demonstrate that death induced by UVB or by ionizing radiation (IR) requires Suppressor of Gamma Response 1 (SOG1), a transcription factor already found to govern many responses to these agents in Arabidopsis. DNA-da...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2010.06.006
更新日期:2010-09-04 00:00:00
abstract::DNA polymerases play a crucial role in the cell cycle due to their involvement in genome replication and repair. Understanding the reaction mechanism by which these polymerases carry out their function can provide insights into these processes. Recently, the crystal structures of human DNA polymerase lambda (Pollambda...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.07.007
更新日期:2008-11-01 00:00:00
abstract::We have used the recently determined crystal structures of Escherichia coli (E. coli) MutS, MutL and MutH to guide construction of 47 amino-acid substitutions in these proteins and analyzed their behavior in mismatch repair and recombination in vitro and in vivo. We find that the active site of the MutH endonuclease i...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(02)00245-8
更新日期:2003-04-02 00:00:00
abstract::Despite detailed knowledge on the genetic network and biochemical properties of most of the nucleotide excision repair (NER) proteins, cell biological analysis has only recently made it possible to investigate the temporal and spatial organization of NER. In contrast to several other DNA damage response mechanisms tha...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2011.04.015
更新日期:2011-07-15 00:00:00
abstract::Double-strand breaks in genomic DNA (DSB) are potentially lethal lesions which separate parts of chromosome arms from their centromeres. Repair of DSB by recombination can generate mutations and further chromosomal rearrangements, making the regulation of recombination and the choice of recombination pathways of the h...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2011.04.002
更新日期:2011-06-10 00:00:00
abstract::We have reported that xeroderma pigmentosum group A (Xpa) gene-knockout mice [Xpa (-/-) mice] are deficient in nucleotide excision repair (NER) and highly sensitive to UV-induced skin carcinogenesis. Although xeroderma pigmentosum group A patients show growth retardation, immature sexual development, and neurological ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.08.003
更新日期:2008-12-01 00:00:00
abstract::The Karpas-620 human myeloma cell line (HMCL) expresses high levels of Cyclin D1 (CCND1), but has a der(8)t(8;11) and a der(14)t(8;14), and not a conventional t(11;14). Fluorescent in situ hybridization (FISH) and array comparative genomic hybridization (aCGH) studies suggest that der(14)t(11;14) from a primary transl...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.11.010
更新日期:2009-03-01 00:00:00
abstract::Ionizing radiation induces clustered DNA damaged sites, defined as two or more lesions formed within one or two helical turns of the DNA through passage of a single radiation track. It is now established that clustered DNA damage sites are found in cells and present a challenge to the repair machinery of the cell but ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2007.07.003
更新日期:2007-12-01 00:00:00
abstract::Srs2 is a 3'-5' DNA helicase that regulates many aspects of DNA metabolism in Saccharomyces cerevisiae. It is best known for its ability to counteract homologous recombination by dismantling Rad51 filaments, but is also involved in checkpoint activation, adaptation and recovery, and in resolution of late recombination...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2011.02.004
更新日期:2011-05-05 00:00:00
abstract::Bacterial MutS2 proteins, consisting of functional domains for ATPase, DNA-binding, and nuclease activities, play roles in DNA recombination and repair. Here we observe a mechanism for generating MutS2 expression diversity in the human pathogen Helicobacter pylori, and identify a unique MutS2 domain responsible for sp...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2017.07.004
更新日期:2017-09-01 00:00:00
abstract::Cells carrying deletions of genes encoding H-class ribonucleases display elevated rates of chromosome instability. The role of these enzymes is to remove RNA-DNA associations including persistent mRNA-DNA hybrids (R-loops) formed during transcription, and ribonucleotides incorporated into DNA during replication. RNase...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2017.02.012
更新日期:2017-04-01 00:00:00
abstract::The accuracy of DNA synthesis depends on the accuracy of the polymerase as well as the quality and concentration(s) of the available 5'-deoxynucleoside-triphosphate DNA precursors (dNTPs). The relationships between dNTPs and error rates have been studied in vitro, but only limited insights exist into these correlation...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2012.10.011
更新日期:2013-01-01 00:00:00
abstract::Deinococcus radiodurans recovering from the effect of acute dose of gamma (gamma) radiation shows a biphasic mechanism of DNA double strands breaks repair that involves an efficient homologous recombination. However, it shows higher sensitivity to near-UV (NUV) than Escherichia coli and lacks RecBC, a DNA strand break...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2007.07.007
更新日期:2008-01-01 00:00:00
abstract::From bacteria to humans, ancient stress responses enable organisms to contend with damage to both the genome and the proteome. These pathways have long been viewed as fundamentally separate responses. Yet recent discoveries from multiple fields have revealed surprising links between the two. Many DNA-damaging agents a...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2018.08.004
更新日期:2018-11-01 00:00:00
abstract::Structure-prone DNA repeats are common components of genomic DNA in all kingdoms of life. In humans, these repeats are linked to genomic instabilities that result in various hereditary disorders, including many cancers. It has long been known that DNA repeats are not only highly polymorphic in length but can also caus...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2015.04.020
更新日期:2015-08-01 00:00:00