Three tandem HRDC domains have synergistic effect on the RecQ functions in Deinococcus radiodurans.

abstract：:Radiation-induced complex double-strand breaks (DSBs) characterised by base lesions, abasic sites or single-strand breaks in close proximity to the break termini, are believed to be a major cause of the biological effects of ionising radiation exposure. It has been hypothesised that complex DSBs pose problems for the ...

journal_title：DNA repair

authors： Dobbs TA,Palmer P,Maniou Z,Lomax ME,O'Neill P

更新日期：2008-08-02 00:00:00

abstract：:The MutS2 homologues have been found widespread in most prokaryotes, which are involved in DNA repair and reactive oxygen species detoxification. The C-terminal small mutS-related (Smr) domain is critical for its endonucleolytic activity. However, the detailed catalytic mechanism is still unclear. In this study, we fi...

journal_title：DNA repair

authors： Zhang H,Xu Q,Lu M,Xu X,Wang Y,Wang L,Zhao Y,Hua Y

更新日期：2014-09-01 00:00:00

abstract：:In eukaryotic cells, DNA associates with histones and exists in the form of a chromatin hierarchy. Thus, it is generally believed that many eukaryotic cellular DNA processing events such as replication, transcription, recombination and DNA repair are influenced by the packaging of DNA into chromatin. This mini-review ...

journal_title：DNA repair

authors： Liu X

更新日期：2015-12-01 00:00:00

abstract：:The oxidation of the thymine methyl group can generate 5-formyluracil (FoU). Template FoU residues are known to miscode, generating base substitution mutations. The repair of the FoU lesion is therefore important in minimizing mutations induced by DNA oxidation. We have studied the repair of FoU in synthetic oligonucl...

journal_title：DNA repair

authors： Liu P,Burdzy A,Sowers LC

更新日期：2003-02-03 00:00:00

abstract：:The DNA damage response is a key factor in the maintenance of genome stability. As such, it is a central axis in sustaining cellular homeostasis in a variety of contexts: development, growth, differentiation, and maintenance of the normal life cycle of the cell. It is now clear that diverse mechanisms encompassing cel...

journal_title：DNA repair

authors： Barzilai A,Biton S,Shiloh Y

更新日期：2008-07-01 00:00:00

abstract：:As haematopoietic stem cell gene therapy utilizing O(6)-methylguanine-DNA-methyltransferase has reached the clinical stage, safety-related questions become increasingly important. These issues concern insertional mutagenesis of viral vectors, the acute toxicity of pre-transplant conditioning protocols and in vivo sele...

journal_title：DNA repair

authors： Sorg UR,Kleff V,Fanaei S,Schumann A,Moellmann M,Opalka B,Thomale J,Moritz T

更新日期：2007-08-01 00:00:00

abstract：:DNA polymerases play a crucial role in the cell cycle due to their involvement in genome replication and repair. Understanding the reaction mechanism by which these polymerases carry out their function can provide insights into these processes. Recently, the crystal structures of human DNA polymerase lambda (Pollambda...

journal_title：DNA repair

authors： Cisneros GA,Perera L,García-Díaz M,Bebenek K,Kunkel TA,Pedersen LG

更新日期：2008-11-01 00:00:00

abstract：:UVB radiation results in the formation of potentially mutagenic photoproducts in the DNA of epidermal skin cells. In vitro approaches have demonstrated that the nucleotide excision repair (NER) machinery removes UV photoproducts from DNA in the form of small (∼30-nt-long), excised, damage-containing DNA oligonucleotid...

journal_title：DNA repair

authors： Choi JH,Han S,Kemp MG

更新日期：2020-02-01 00:00:00

abstract：:Xeroderma pigmentosum (XP) is a genetic disorder associated with defects in nucleotide excision repair, a pathway that eliminates a wide variety of helix-distorting DNA lesions, including ultraviolet-induced pyrimidine dimers. In addition to skin diseases in sun-exposed areas, approximately 25% of XP patients develop ...

journal_title：DNA repair

authors： Mori T,Nakane H,Iwamoto T,Krokidis MG,Chatgilialoglu C,Tanaka K,Kaidoh T,Hasegawa M,Sugiura S

更新日期：2019-08-01 00:00:00

abstract：:Cancer-prone diseases ataxia-telangiectasia (AT), Nijmegen breakage syndrome (NBS) and ataxia-telangiectasia-like disorder (ATLD) are defective in the repair of DNA double-stranded break (DSB). On the other hand, arsenic (As) has been reported to cause DSB and to be involved in the occurrence of skin, lung and bladder...

journal_title：DNA repair

authors： Yuan SS,Su JH,Hou MF,Yang FW,Zhao S,Lee EY

更新日期：2002-02-28 00:00:00

abstract：:The accuracy of DNA synthesis depends on the accuracy of the polymerase as well as the quality and concentration(s) of the available 5'-deoxynucleoside-triphosphate DNA precursors (dNTPs). The relationships between dNTPs and error rates have been studied in vitro, but only limited insights exist into these correlation...

journal_title：DNA repair

authors： Schaaper RM,Mathews CK

更新日期：2013-01-01 00:00:00

abstract：:Nucleotide excision repair (NER) is a DNA repair pathway, which eliminates various types of helix-distorting DNA damage including some forms of oxidative damage and UV-induced photoproducts. To understand why patients with NER-defective disorders develop progressive neurological abnormalities, we investigated NER capa...

journal_title：DNA repair

authors： Yamamoto A,Nakamura Y,Kobayashi N,Iwamoto T,Yoshioka A,Kuniyasu H,Kishimoto T,Mori T

更新日期：2007-05-01 00:00:00

abstract：:The ubiquitin-proteasome pathway plays an important role in DNA damage signaling and repair by facilitating the recruitment and activation of DNA repair factors and signaling proteins at sites of damaged chromatin. Proteasome activity is generally not thought to be required for activation of apical signaling kinases i...

journal_title：DNA repair

authors： Sakasai R,Teraoka H,Tibbetts RS

更新日期：2010-01-02 00:00:00

abstract：:Human papillomavirus (HPV) is associated with the development of head and neck squamous cell carcinomas (HNSC). Cisplatin is used to treat HNSC and induces DNA adducts including interstrand crosslinks (ICLs). Previous reports have shown that HPV positive HNSC patients respond better to cisplatin therapy. Our previous ...

journal_title：DNA repair

authors： Conner KL,Shaik AN,Ekinci E,Kim S,Ruterbusch JJ,Cote ML,Patrick SM

更新日期：2020-03-01 00:00:00

abstract：:In the midst of the post-war turmoil in Japan, I fortunately followed a path to become a scientist. Sometime at an early stage of my career, I encountered the problem of the cellular response to DNA damage and had the chance to discover a DNA repair enzyme. This event greatly influenced the subsequent course of my res...

journal_title：DNA repair

authors： Sekiguchi M

更新日期：2006-06-10 00:00:00

abstract：:Gene amplification, a key mechanism for oncogene activation and drug resistance in tumour cells, involves the generation and joining of DNA double-strand breaks. Amplified DNA can be carried either on intra-chromosomal arrays or on extra-chromosomal elements (double minutes). We previously showed that, in rodent cells...

journal_title：DNA repair

authors： Salzano A,Kochiashvili N,Nergadze SG,Khoriauli L,Smirnova A,Ruiz-Herrera A,Mondello C,Giulotto E

更新日期：2009-01-01 00:00:00

abstract：:Me-lex is a sequence-specific alkylating agent synthesized to preferentially (>90%) generate N3-methyladenine (3-mA) in the minor groove of double-strand DNA, in A-T rich regions. In this paper we investigated the effect of XRCC1 deficiency in the processing of 3-mA adducts generated by Me-lex, through the molecular a...

journal_title：DNA repair

authors： Russo D,Fronza G,Ottaggio L,Monti P,Perfumo C,Inga A,Iyer P,Gold B,Menichini P

更新日期：2010-07-01 00:00:00

abstract：:Reactive oxygen and nitrogen species (RONS) are formed as byproducts of many endogenous cellular processes, in response to infections, and upon exposure to various environmental factors. An increase in RONS can saturate the antioxidation system and leads to oxidative stress. Consequently, macromolecules are targeted f...

journal_title：DNA repair

authors： Alnajjar KS,Sweasy JB

更新日期：2019-04-01 00:00:00

abstract：:Structure-prone DNA repeats are common components of genomic DNA in all kingdoms of life. In humans, these repeats are linked to genomic instabilities that result in various hereditary disorders, including many cancers. It has long been known that DNA repeats are not only highly polymorphic in length but can also caus...

journal_title：DNA repair

authors： Shah KA,Mirkin SM

更新日期：2015-08-01 00:00:00

abstract：:There is an increasing demand for phenotyping assays in the field of human functional genetics. DNA repair activity is representative of this functional approach, being seen as a valuable biomarker related to cancer risk. Repair activity is evaluated by incubating a cell extract with a DNA substrate containing lesions...

journal_title：DNA repair

authors： Azqueta A,Langie SA,Slyskova J,Collins AR

更新日期：2013-11-01 00:00:00

abstract：:Expansion of a CGG-repeat tract in the 5' UTR of FMR1 is responsible for the Fragile X-related disorders (FXDs), FXTAS, FXPOI and FXS. Previous work in a mouse model of these disorders has implicated proteins in the base excision and the mismatch repair (MMR) pathways in the expansion mechanism. However, the precise r...

journal_title：DNA repair

authors： Gazy I,Hayward B,Potapova S,Zhao X,Usdin K

更新日期：2019-02-01 00:00:00

abstract：:DNA mismatch repair is an evolutionarily conserved repair pathway that corrects replication errors. In most prokaryotes and all eukaryotes, the mismatch repair protein MutL is a sequence-unspecific endonuclease that nicks the newly synthesized strand and marks it for repair. Although the sequence of the endonuclease d...

journal_title：DNA repair

authors： Liu L,Ortiz Castro MC,Rodríguez González J,Pillon MC,Guarné A

更新日期：2019-01-01 00:00:00

abstract：:The Karpas-620 human myeloma cell line (HMCL) expresses high levels of Cyclin D1 (CCND1), but has a der(8)t(8;11) and a der(14)t(8;14), and not a conventional t(11;14). Fluorescent in situ hybridization (FISH) and array comparative genomic hybridization (aCGH) studies suggest that der(14)t(11;14) from a primary transl...

journal_title：DNA repair

authors： Dib A,Glebov OK,Shou Y,Singer RH,Kuehl WM

更新日期：2009-03-01 00:00:00

abstract：:Translesion DNA polymerases (TLS pols) play critical roles in defense mechanisms against genotoxic agents. The defects or mutations of TLS pols are predicted to result in hypersensitivity of cells to environmental mutagens. In this study, human cells expressing DNA polymerase ζ (Pol ζ) variants with low fidelity or we...

journal_title：DNA repair

authors： Suzuki T,Grúz P,Honma M,Adachi N,Nohmi T

更新日期：2016-09-01 00:00:00

abstract：:The cellular response to DNA damage is essential for maintenance of genomic stability. MDC1 is a key member of the DNA damage response. It is an adaptor protein that binds and recruits proteins to sites of DNA damage, a crucial step for a proper response. MDC1 contains several protein-protein interacting modules, incl...

journal_title：DNA repair

authors： Strauss C,Halevy T,Macarov M,Argaman L,Goldberg M

更新日期：2011-08-15 00:00:00

abstract：:Methylation of the O(6)-methylguanine-DNA-methyltransferase (MGMT) promoter is associated with G:C to A:T transitions in the p53 gene in various human cancers, including lung cancer. In tumors with p53 mutation, MGMT promoter methylation is more common in advanced tumors than in early tumors. However, in tumors with w...

journal_title：DNA repair

authors： Lai JC,Cheng YW,Goan YG,Chang JT,Wu TC,Chen CY,Lee H

更新日期：2008-08-02 00:00:00

abstract：:The integrity of cellular genome is continuously challenged by endogenous and exogenous DNA damaging agents. If DNA damage is not removed in a timely fashion the replisome may stall at DNA lesions, causing fork collapse and genetic instability. Base excision DNA repair (BER) is the most important pathway for the remov...

journal_title：DNA repair

authors： Jaiswal AS,Williamson EA,Srinivasan G,Kong K,Lomelino CL,McKenna R,Walter C,Sung P,Narayan S,Hromas R

更新日期：2020-02-01 00:00:00

abstract：:Quantitating relative (32)P-band intensity in gels is desired, e.g., to study primer-extension kinetics of DNA polymerases (DNAPs). Following imaging, multiple (32)P-bands are often present in lanes. Though individual bands appear by eye to be simple and well-resolved, scanning reveals they are actually skewed-Gaussia...

journal_title：DNA repair

authors： Sholder G,Loechler EL

更新日期：2015-01-01 00:00:00

abstract：:The XPB DNA helicase, a subunit of the basal transcription factor TFIIH, is also involved in nucleotide excision repair (NER). We examined recruitment of NER proteins in XP-B cells from patients with mild or severe xeroderma pigmentosum (XP) having different XPB mutations using local UV-irradiation through filters wit...

journal_title：DNA repair

authors： Oh KS,Imoto K,Boyle J,Khan SG,Kraemer KH

更新日期：2007-09-01 00:00:00

abstract：:Misalignment of a nascent strand and the use of an alternative template during DNA replication, a process termed "template-switching", can give rise to frequent mutations and genetic rearrangements. Mutational hotspots are frequently found associated with imperfect inverted repeats ("quasipalindromes" or "QPs") in man...

journal_title：DNA repair

authors： Laranjo LT,Gross SJ,Zeiger DM,Lovett ST

更新日期：2017-09-01 00:00:00