Abstract：
:The accuracy of DNA synthesis depends on the accuracy of the polymerase as well as the quality and concentration(s) of the available 5'-deoxynucleoside-triphosphate DNA precursors (dNTPs). The relationships between dNTPs and error rates have been studied in vitro, but only limited insights exist into these correlations during in vivo replication. We have investigated this issue in the bacterium Escherichia coli by analyzing the mutational properties of dcd and ndk strains. These strains, defective in dCTP deaminase and nucleoside diphosphate kinase, respectively, are characterized by both disturbances of dNTP pools and a mutator phenotype. ndk strains have been studied before, but were included in this study, as controversies exist regarding the source of its mutator phenotype. We show that dcd strains suffer from increased intracellular levels of dCTP (4-fold) and reduced levels of dGTP (2-fold), while displaying, as measured using a set of lacZ reversion markers in a mismatch-repair defective (mutL) background, a strong mutator effect for G·C→T·A and A·T→T·A transversions (27- and 42-fold enhancement, respectively). In contrast, ndk strains possess a lowered dATP level (4-fold) and modestly enhanced dCTP level (2-fold), while its mutator effect is specific for just the A·T→T·A transversions. The two strains also display differential mutability for rifampicin-resistant mutants. Overall, our analysis reveals for both strains a satisfactory correlation between dNTP pool alterations and the replication error rates, and also suggests that a minimal explanation for the ndk mutator does not require assumptions beyond the predicted effect of the dNTP pools.
journal_name
DNA Repair (Amst)journal_title
DNA repairauthors
Schaaper RM,Mathews CKdoi
10.1016/j.dnarep.2012.10.011subject
Has Abstractpub_date
2013-01-01 00:00:00pages
73-9issue
1eissn
1568-7864issn
1568-7856pii
S1568-7864(12)00279-0journal_volume
12pub_type
杂志文章相关文献
DNA REPAIR文献大全abstract：:Double-strand breaks in genomic DNA (DSB) are potentially lethal lesions which separate parts of chromosome arms from their centromeres. Repair of DSB by recombination can generate mutations and further chromosomal rearrangements, making the regulation of recombination and the choice of recombination pathways of the h...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2011.04.002
更新日期：2011-06-10 00:00:00
abstract：:Base excision repair of oxidized DNA in human cells is initiated by several DNA glycosylases with overlapping substrate specificity. The human endonuclease VIII homologue NEIL1 removes a broad spectrum of oxidized pyrimidine and purine lesions. In this study of NEIL1 we have identified several key residues, located in...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2012.07.002
更新日期：2012-09-01 00:00:00
abstract：:Processivity clamps that hold DNA polymerases to DNA for processivity were the first proteins known to encircle the DNA duplex. At the time, polymerase processivity was thought to be the only function of ring shaped processivity clamps. But studies from many laboratories have identified numerous proteins that bind and...
journal_title：DNA repair
pub_type： 杂志文章,评审
doi：10.1016/j.dnarep.2015.01.015
更新日期：2015-05-01 00:00:00
abstract：:Srs2 is a 3'-5' DNA helicase that regulates many aspects of DNA metabolism in Saccharomyces cerevisiae. It is best known for its ability to counteract homologous recombination by dismantling Rad51 filaments, but is also involved in checkpoint activation, adaptation and recovery, and in resolution of late recombination...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2011.02.004
更新日期：2011-05-05 00:00:00
abstract：:By combining mutations in DNA repair genes, important and unexpected interactions between different repair pathways can be discovered. In this study, we identified a novel link between mismatch repair (MMR) genes and postreplication repair (PRR) in Saccharomyces cerevisiae. Strains lacking Rad5 (HLTF in mammals), a pr...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2020.102870
更新日期：2020-01-01 00:00:00
abstract：:PriA, a 3'-->5' superfamily 2 DNA helicase, acts to remodel stalled replication forks and as a specificity factor for origin-independent assembly of a new replisome at the stalled fork. The ability of PriA to initiate replication at stalled forked structures ensures complete genome replication and helps to protect the...
journal_title：DNA repair
pub_type： 杂志文章,评审
doi：10.1016/j.dnarep.2009.12.009
更新日期：2010-03-02 00:00:00
abstract：:Double-strand breaks (DSBs) are among the most lethal DNA lesions, and a variety of pathways have evolved to manage their repair in a timely fashion. One such pathway is homologous recombination (HR), in which information from an undamaged donor site is used as a template for repair. Although many of the biochemical s...
journal_title：DNA repair
pub_type： 杂志文章,评审
doi：10.1016/j.dnarep.2017.06.012
更新日期：2017-08-01 00:00:00
abstract：:During DNA synthesis in vitro using dNTP and rNTP concentrations present in vivo, yeast replicative DNA polymerases α, δ and ɛ (Pols α, δ and ɛ) stably incorporate rNTPs into DNA. rNTPs are also incorporated during replication in vivo, and they are repaired in an RNase H2-dependent manner. In strains encoding a mutato...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2011.02.001
更新日期：2011-05-05 00:00:00
abstract：:Deinococcus radiodurans recovering from the effect of acute dose of gamma (gamma) radiation shows a biphasic mechanism of DNA double strands breaks repair that involves an efficient homologous recombination. However, it shows higher sensitivity to near-UV (NUV) than Escherichia coli and lacks RecBC, a DNA strand break...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2007.07.007
更新日期：2008-01-01 00:00:00
abstract：:Several types of DNA lesion are induced after ionizing irradiation (IR) of which double strand breaks (DSBs) are expected to be the most lethal, although single strand breaks (SSBs) and DNA base damages are quantitatively in the majority. Proteins of the base excision repair (BER) pathway repair these numerous lesions...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2008.11.008
更新日期：2009-03-01 00:00:00
abstract：:In response to the threat of DNA damage, cells exhibit a dramatic and multi-factorial response spanning from transcriptional changes to protein modifications, collectively known as the DNA damage response (DDR). Here, we review the literature surrounding the transcriptional response to DNA damage. We review difference...
journal_title：DNA repair
pub_type： 杂志文章,评审
doi：10.1016/j.dnarep.2019.05.002
更新日期：2019-07-01 00:00:00
abstract：:Me-lex is a sequence-specific alkylating agent synthesized to preferentially (>90%) generate N3-methyladenine (3-mA) in the minor groove of double-strand DNA, in A-T rich regions. In this paper we investigated the effect of XRCC1 deficiency in the processing of 3-mA adducts generated by Me-lex, through the molecular a...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2010.03.016
更新日期：2010-07-01 00:00:00
abstract：:Chk1 is a protein kinase that acts as a key signal transducer within the complex network responsible of the cellular response to different DNA damages. It is a conserved element along the eukaryotic kingdom, together with a second checkpoint kinase, called Chk2/Rad53. In fact, all organisms studied so far carried at l...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2009.01.023
更新日期：2009-06-04 00:00:00
abstract：:Gene amplification, a key mechanism for oncogene activation and drug resistance in tumour cells, involves the generation and joining of DNA double-strand breaks. Amplified DNA can be carried either on intra-chromosomal arrays or on extra-chromosomal elements (double minutes). We previously showed that, in rodent cells...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2008.08.015
更新日期：2009-01-01 00:00:00
abstract：:Pathways for tolerating and repairing DNA-protein crosslinks (DPCs) are poorly defined. We used transposon mutagenesis and candidate gene approaches to identify DPC-hypersensitive Escherichia coli mutants. DPCs were induced by azacytidine (aza-C) treatment in cells overexpressing cytosine methyltransferase; hypersensi...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2015.01.016
更新日期：2015-04-01 00:00:00
abstract：:The First joint meeting of the German DGDR (German Society for Research on DNA Repair) and the French SFTG (French Society of Genotoxicology) on DNA Repair was held in Toulouse, France, from September 15 to 19, 2007. It was organized by Lisa Wiesmüller and Bernard Salles together with the scientific committee consisti...
journal_title：DNA repair
pub_type：
doi：10.1016/j.dnarep.2007.12.007
更新日期：2008-04-02 00:00:00
abstract：:Interaction between MutS and the replication factor β clamp has been extensively studied in a Mismatch Repair context; however, its functional consequences are not well understood. We have analyzed the role of the MutS-β clamp interaction in Pseudomonas aeruginosa by characterizing a β clamp binding motif mutant, deno...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2012.01.015
更新日期：2012-05-01 00:00:00
abstract：:The RAD52 gene is essential for homology-dependent repair of double-strand breaks in Saccharomyces cerevisiae. Rad52 forms complexes with Rad51, replication protein A (RPA) or Rad59 and its presence is essential for the formation of Rad51-Rad52-Rad59 and RPA-Rad52-Rad59 complexes. The N-terminal region of Rad52, which...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/s1568-7864(03)00121-6
更新日期：2003-10-07 00:00:00
abstract：:Methylation of the O(6)-methylguanine-DNA-methyltransferase (MGMT) promoter is associated with G:C to A:T transitions in the p53 gene in various human cancers, including lung cancer. In tumors with p53 mutation, MGMT promoter methylation is more common in advanced tumors than in early tumors. However, in tumors with w...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2008.04.016
更新日期：2008-08-02 00:00:00
abstract：:DNA damage and ageing share expression changes involving alterations in many aspects of metabolism, suppression of growth and upregulation of defence and genome maintenance systems. "Omics" technologies have permitted large-scale parallel measurements covering global cellular constituents and aided the identification ...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2013.08.016
更新日期：2013-11-01 00:00:00
abstract：:Quantitating relative (32)P-band intensity in gels is desired, e.g., to study primer-extension kinetics of DNA polymerases (DNAPs). Following imaging, multiple (32)P-bands are often present in lanes. Though individual bands appear by eye to be simple and well-resolved, scanning reveals they are actually skewed-Gaussia...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2014.10.001
更新日期：2015-01-01 00:00:00
abstract：:Mitochondria are membrane-bound organelles found in eukaryotic cells where they generate energy through the respiratory chain. They contain their own genome that encodes genes critical to the mitochondrial function, but most of their protein content is synthetized from nuclear encoded genes. Damages to the mtDNA can c...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2019.102684
更新日期：2019-10-01 00:00:00
abstract：:Non-B DNA conformations adopted by certain types of DNA sequences promote genetic instabilities, especially gross rearrangements including translocations. We conclude the following: (a) slipped (hairpin) structures, cruciforms, triplexes, tetraplexes and i-motifs, and left-handed Z-DNA are formed in chromosomes and el...
journal_title：DNA repair
pub_type： 杂志文章,评审
doi：10.1016/j.dnarep.2006.05.032
更新日期：2006-09-08 00:00:00
abstract：:Cellular DNA is constantly challenged by damage-inducing factors derived from exogenous or endogenous sources. Thus, to protect against DNA damage, cells have evolved complex and finely regulated mechanisms collectively known as DNA-damage response (DDR). However, DNA repair in eukaryotes does not occur merely in nake...
journal_title：DNA repair
pub_type： 杂志文章,评审
doi：10.1016/j.dnarep.2019.06.009
更新日期：2019-08-01 00:00:00
abstract：:TFIIH is a multiprotein complex that has a central role in the RNA pol II mediated transcription, in DNA repair and in the control of the cell cycle. Mutations in some components of TFIIH are associated with three hereditary human syndromes: xeroderma pigmentosum (XP), Cockayne syndrome (CS) and trichothiodystrophy (T...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/s1568-7864(02)00012-5
更新日期：2002-05-30 00:00:00
abstract：:Exposure to ionizing radiation results in a variety of genome rearrangements that have been linked to tumor formation. Many of these rearrangements are thought to arise from the repair of double-strand breaks (DSBs) by several mechanisms, including homologous recombination (HR) between repetitive sequences dispersed t...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2008.02.003
更新日期：2008-05-03 00:00:00
abstract：:The proposed mechanism for transcription coupled nucleotide excision repair (TCR) invokes RNA polymerase (RNAP) blocked at a DNA lesion as a signal to initiate repair. In Escherichia coli, TCR requires the interaction of RNAP with a transcription-repair coupling factor encoded by the mfd gene. The interaction between ...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2007.04.002
更新日期：2007-10-01 00:00:00
abstract：:Maintenance of DNA integrity is vital for all of the living organisms. Consequence of DNA damaging ranges from, introducing harmless synonymous mutations, to causing disease-associated mutations, genome instability, and cell death. A cell cycle protein cyclin D1 is an established cancer-driving protein. However, contr...
journal_title：DNA repair
pub_type： 杂志文章,评审
doi：10.1016/j.dnarep.2016.04.011
更新日期：2016-06-01 00:00:00
abstract：:Microscopically visible gammaH2AX foci signify the presence of DNA double-strand breaks (dsbs) in irradiated cells. However, large foci are also observed in untreated tumour cells, and high numbers reduce the sensitivity for detecting drug or radiation-induced DNA breaks. SW756 cervical carcinoma cells that express ab...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2006.05.040
更新日期：2006-08-13 00:00:00
abstract：:Deoxyinosine (dI) is produced in DNA by the hydrolytic or nitrosative deamination of deoxyadenosine. It is excised in a repair pathway that is initiated by endonuclease V, the product of the nfi gene. The repair was studied in vivo using high-efficiency oligonucleotide transformation mediated by the Beta protein of ba...
journal_title：DNA repair
pub_type： 杂志文章
doi：10.1016/j.dnarep.2007.09.010
更新日期：2008-02-01 00:00:00
