Abstract:
:TFIIH is a multiprotein complex that has a central role in the RNA pol II mediated transcription, in DNA repair and in the control of the cell cycle. Mutations in some components of TFIIH are associated with three hereditary human syndromes: xeroderma pigmentosum (XP), Cockayne syndrome (CS) and trichothiodystrophy (TTD). The p62 protein is a structural component of the TFIIH core and no syndromes have been identified up to date by mutations in this human gene. In this work we report the molecular and genetic characterization of the Drosophila melanogaster p62 gene (Dmp62). The Dmp62 gene product shows high identity with its human and mouse homologues. Using computer analysis we identified several common motifs in the p62 proteins from different organisms, suggesting that these motifs could be involved in possible protein-protein interactions within the TFIIH complex or with other transcription and DNA repair factors. The Dmp62 transcript is expressed at similar levels throughout development, although there is a significant increase of the transcript level during the late embryogenesis and in the adult male. The analysis of a Drosophila line with a P-element enhancer trap insertion at the Dmp62 5'-UTR that directs the lac-Z expression from the Dmp62 promoter, showed a high level of expression in the gut, the testis and the pericardial cells. A P-element that disrupts the Dmp62 gene (Dmp62mut) produces early embryo lethality in homozygous flies. Heterozygous Dmp62mut larvae are more sensitive to UV light irradiation, and those individuals that are able to develop into adults have severe abdominal cuticular damage after UV light irradiation.
journal_name
DNA Repair (Amst)journal_title
DNA repairauthors
Castro J,Merino C,Zurita Mdoi
10.1016/s1568-7864(02)00012-5keywords:
subject
Has Abstractpub_date
2002-05-30 00:00:00pages
359-68issue
5eissn
1568-7864issn
1568-7856pii
S1568786402000125journal_volume
1pub_type
杂志文章相关文献
DNA REPAIR文献大全abstract::The efficiency and fidelity of nucleotide incorporation and next-base extension by DNA polymerase (pol) κ past N(2)-ethyl-Gua were measured using steady-state and rapid kinetic analyses. DNA pol κ incorporated nucleotides and extended 3' termini opposite N(2)-ethyl-Gua with measured efficiencies and fidelities similar...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2010.09.007
更新日期:2011-01-02 00:00:00
abstract::Endonuclease III (EndoIII) is nearly ubiquitous in all three domains of life. EndoIII family proteins exhibit a bifunctional (glycosylase/lyase) activity on oxidative/saturated pyrimidine bases, such as thymine glycol. Previous studies on EndoIII homologs have reported the presence of important residues involved in su...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2020.102859
更新日期:2020-06-01 00:00:00
abstract::RecQ-like helicases are a highly conserved protein family that functions during DNA repair and, when mutated in humans, is associated with cancer and/or premature aging syndromes. The budding yeast RecQ-like helicase Sgs1 has important functions in double-strand break (DSB) repair of exogenously induced breaks, as wel...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2014.12.004
更新日期:2015-02-01 00:00:00
abstract::Specialized DNA polymerases are required to bypass DNA damage lesions that would otherwise cause replication arrest and cell death. When operating on non-canonical templates, such as undamaged DNA or on non-cognate lesions, these polymerases exhibit considerably reduced fidelity, resulting in the generation of mutatio...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2005.01.005
更新日期:2005-05-02 00:00:00
abstract::RAre DAmage and Repair sequencing (RADAR-seq) is a highly adaptable sequencing method that enables the identification and detection of rare DNA damage events for a wide variety of DNA lesions at single-molecule resolution on a genome-wide scale. In RADAR-seq, DNA lesions are replaced with a patch of modified bases tha...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2019.06.007
更新日期:2019-08-01 00:00:00
abstract::The RAD6/RAD18 heterodimer promotes translesion synthesis via the monoubiquitination of the DNA sliding clamp, PCNA. In S. cerevisiae, a second complex, UBC13/MMS2/RAD5, can extend this single ubiquitin with a non-canonical lysine 63-linked chain. This polyubiquitination step is required for an error-free mode of bypa...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2004.12.002
更新日期:2005-04-04 00:00:00
abstract::DNA double-strand breaks (DSBs) in yeast are repaired by homologous recombination (HR) and non-homologous end-joining (NHEJ). Rad51 forms nucleoprotein filaments at processed broken ends that effect strand exchange, forming heteroduplex DNA (hDNA) that gives rise to a gene conversion tract. We hypothesized that excess...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2005.03.003
更新日期:2005-06-08 00:00:00
abstract::The DNA damage checkpoint is a surveillance mechanism activated by DNA lesions and devoted to the maintenance of genome stability. It is considered as a signal transduction cascade, involving a sensing step, the activation of a set of protein kinases and the transmission and amplification of the damage signal through ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2004.06.019
更新日期:2004-12-02 00:00:00
abstract::The extremely radiation resistant bacterium, Deinococcus radiodurans, contains a spectrum of genes that encode for multiple activities that repair DNA damage. We have cloned and expressed the product of three predicted uracil-DNA glycosylases to determine their biochemical function. DR0689 is a homologue of the Escher...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2003.10.011
更新日期:2004-02-03 00:00:00
abstract::Although correlative studies demonstrate a reduction in the expression of apurinic/apyrimidinic endonuclease/redox effector factor (Ape1/Ref-1 or Ape1) in neural tissues after neuronal insult, the role of Ape1 in regulating neurotoxicity remains to be elucidated. To address this issue, we examined the effects of reduc...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2004.11.006
更新日期:2005-03-02 00:00:00
abstract::DNA interstrand crosslinks (ICLs) are highly toxic lesions that covalently link both strands of DNA and distort the DNA helix. Crosslinking agents have been shown to stall DNA replication and failure to repair ICL lesions before encountered by replication forks may induce severe DNA damage. Most knowledge of the ICL r...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2012.09.010
更新日期:2012-12-01 00:00:00
abstract::Mononucleotide microsatellites are tandem repeats of a single base pair, abundant within coding exons and frequent sites of mutation in the human genome. Because the repeated unit is one base pair, multiple mechanisms of insertion/deletion (indel) mutagenesis are possible, including strand-slippage, dNTP-stabilized, a...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2015.02.016
更新日期:2015-05-01 00:00:00
abstract::Mice defective for the Polk gene, which encodes DNA polymerase kappa, are viable and do not manifest obvious phenotypes. The present studies document a spontaneous mutator phenotype in Polk(-/-) mice. The initial indication of enhanced spontaneous mutations in these mice came from the serendipitous observation of a po...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2009.09.003
更新日期:2009-12-03 00:00:00
abstract::Nucleotide excision repair and reversal of pyrimidine dimers by photolyase (photoreactivation) are two major pathways to remove UV-lesions from DNA. Here, it is discussed how lesions are recognized and removed when the DNA is condensed into nucleosomes. During the recent years it was shown that nucleosomes inhibit pho...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2005.04.005
更新日期:2005-07-28 00:00:00
abstract::Bacterial RarA is thought to play crucial roles in the cellular response to blocked replication forks. We show that lack of Bacillus subtilis RarA renders cells very sensitive to H2O2, but not to methyl methane sulfonate or 4-nitroquinoline-1-oxide. RarA is epistatic to RecA in response to DNA damage. Inactivation of ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2019.03.010
更新日期:2019-06-01 00:00:00
abstract::During DNA synthesis in vitro using dNTP and rNTP concentrations present in vivo, yeast replicative DNA polymerases α, δ and ɛ (Pols α, δ and ɛ) stably incorporate rNTPs into DNA. rNTPs are also incorporated during replication in vivo, and they are repaired in an RNase H2-dependent manner. In strains encoding a mutato...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2011.02.001
更新日期:2011-05-05 00:00:00
abstract::Genome integrity is constantly challenged by endo- and exogenous DNA-damaging factors. The influence of genotoxic agents causes an accumulation of DNA lesions, which if not repaired, become mutations that can cause various abnormalities in a cell metabolism. The main pathway of DSB repair, which is based on non-homolo...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2015.02.018
更新日期:2015-04-01 00:00:00
abstract::The DNA damage response is a key factor in the maintenance of genome stability. As such, it is a central axis in sustaining cellular homeostasis in a variety of contexts: development, growth, differentiation, and maintenance of the normal life cycle of the cell. It is now clear that diverse mechanisms encompassing cel...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2008.03.005
更新日期:2008-07-01 00:00:00
abstract::Primary human somatic cells grown in culture divide a finite number of times, exhibiting progressive changes in metabolism and morphology before cessation of cycling. This telomere-initiated cellular senescence occurs because cells have halted production of telomerase, a DNA polymerase required for stabilization of ch...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2011.10.003
更新日期:2012-01-02 00:00:00
abstract::Xeroderma pigmentosum (XP) genetic complementation group C (XP-C) is the most common form of the disease worldwide. Thirty-four distinct genetic defects have been identified in 45 XP-C patients. Further identification of such defects and the frequency of their occurrence offers the potential of generating diagnostic a...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2006.09.009
更新日期:2007-01-04 00:00:00
abstract::The detection of γ-H2AX focus is one of the most sensitive ways to monitor DNA double-strand breaks (DSBs). Although changes in γ-H2AX activity have been studied in tumor cells in colorectal cancer (CRC), changes in peripheral blood lymphocytes (PBLs) have not been examined previously. We hypothesize that higher level...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2017.03.001
更新日期:2017-05-01 00:00:00
abstract::3-Nitrobenzanthrone (3-NBA) is a highly mutagenic compound and possible human carcinogen found in diesel exhaust. 3-NBA forms bulky DNA adducts following metabolic activation and induces predominantly G:CT:A transversions in a variety of experimental systems. Here we investigated the influence of nucleotide excision r...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2015.11.004
更新日期:2016-03-01 00:00:00
abstract::Deinococcus radiodurans survives extremely high doses of ionizing and ultraviolet radiation and treatment with various DNA-damaging chemicals. As an effort to identify and characterize proteins that function in DNA repair in this organism, we have studied the protein encoded by locus DR1572. This gene is predicted to ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.12.011
更新日期:2009-05-01 00:00:00
abstract::The hereditary breast and ovarian cancer predisposition genes, BRCA1 and BRCA2, participate in the repair of DNA double strand breaks by homologous recombination. Circumstantial evidence implicates these genes in recombinational responses to DNA polymerase stalling during the S phase of the cell cycle. These responses...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2007.02.020
更新日期:2007-07-01 00:00:00
abstract::The mechanisms that allow to circumvent replicative stress, and to resume DNA synthesis are poorly understood in Bacillus subtilis. To study the role of the diadenylate cyclase DisA and branch migration translocase (BMT) RadA/Sms in restarting a stalled replication fork, we nicked and broke the circular chromosome of ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2017.09.006
更新日期:2017-11-01 00:00:00
abstract::Nucleotide excision repair (NER) is a conserved DNA repair mechanism capable of removing a variety of helix-distorting DNA lesions. Rad26, a member of the Swi2/Snf2 superfamily of proteins, has been shown to be involved in a specialized NER process called transcription coupled NER. Rad16, another member of the same pr...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2007.05.005
更新日期:2007-11-01 00:00:00
abstract::Telomeres play an important role in protecting the ends of chromosomes and preventing chromosome fusion. We have previously demonstrated that double-strand breaks near telomeres in mammalian cells result in either the addition of a new telomere at the site of the break, termed chromosome healing, or sister chromatid f...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.04.004
更新日期:2008-08-02 00:00:00
abstract::DNA polymerases beta (pol beta ) and eta (pol eta ) are the only two eukaryotic polymerases known to efficiently bypass cisplatin and oxaliplatin adducts in vitro. Frameshift errors are an important aspect of mutagenesis. We have compared the types of frameshifts that occur during translesion synthesis past cisplatin ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(02)00150-7
更新日期:2002-12-05 00:00:00
abstract::The RecQ family of DNA helicases performs essential functions in the maintenance of genomic stability in all organisms. In Deinococcus radiodurans, DR1289 is a special member of RecQ family with unique arrangement of three tandem HRDC domains in the C-terminus. A dr1289 mutant is hypersensitive to gamma-irradiation, U...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2006.09.006
更新日期:2007-02-04 00:00:00
abstract::Base excision repair of oxidized DNA in human cells is initiated by several DNA glycosylases with overlapping substrate specificity. The human endonuclease VIII homologue NEIL1 removes a broad spectrum of oxidized pyrimidine and purine lesions. In this study of NEIL1 we have identified several key residues, located in...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2012.07.002
更新日期:2012-09-01 00:00:00