Abstract:
:DNA double-strand breaks (DSBs) in yeast are repaired by homologous recombination (HR) and non-homologous end-joining (NHEJ). Rad51 forms nucleoprotein filaments at processed broken ends that effect strand exchange, forming heteroduplex DNA (hDNA) that gives rise to a gene conversion tract. We hypothesized that excess Rad51 would increase gene conversion tract lengths. We found that excess Rad51 reduced DSB-induced HR but did not alter tract lengths or other outcomes including rates of crossovers, break-induced replication, or chromosome loss. Thus, excess Rad51 appears to influence DSB-induced HR at an early stage. MAT heterozygosity largely mitigated the inhibitory effect of excess Rad51 on allelic HR, but not direct repeat HR. Excess Rad52 had no effect on DSB-induced HR efficiency or outcome, nor did it mitigate the dominant negative effects of excess Rad51. Excess Rad51 had little effect on DSB-induced lethality in wild-type cells, but it did enhance lethality in yku70Delta mutants. Interestingly, dnl4Delta showed marked DSB-induced lethality but this was not further enhanced by excess Rad51. The differential effects of yku70Delta and dnl4Delta indicate that the enhanced killing with excess Rad51 in yku70Delta is not due to its NHEJ defect, but may reflect its defect in end-protection and/or its inability to escape from checkpoint arrest. Srs2 displaces Rad51 from nucleoprotein filaments in vitro, suggesting that excess Rad51 might antagonize Srs2. We show that excess Rad51 does not reduce survival of wild-type cells treated with methylmethane sulfonate (MMS), or cells suffering a single DSB. In contrast, excess Rad51 sensitized srs2Delta cells to both MMS and a single DSB. These results support the idea that excess Rad51 antagonizes Srs2, and underscores the importance of displacing Rad51 from nucleoprotein filaments to achieve optimum repair efficiency.
journal_name
DNA Repair (Amst)journal_title
DNA repairauthors
Paffett KS,Clikeman JA,Palmer S,Nickoloff JAdoi
10.1016/j.dnarep.2005.03.003keywords:
subject
Has Abstractpub_date
2005-06-08 00:00:00pages
687-98issue
6eissn
1568-7864issn
1568-7856pii
S1568-7864(05)00071-6journal_volume
4pub_type
杂志文章相关文献
DNA REPAIR文献大全abstract::All organisms rely on integrated networks to repair DNA double-strand breaks (DSBs) in order to preserve the integrity of the genetic information, to re-establish replication, and to ensure proper chromosomal segregation. Genetic, cytological, biochemical and structural approaches have been used to analyze how Bacillu...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2012.12.005
更新日期:2013-03-01 00:00:00
abstract::The linear plasmid (pPac1-2) encoded killer toxin (PaT) of the yeast Pichia acaciae arrests sensitive Saccharomyces cerevisiae cells in the S-phase of the cell cycle and induces mutations. Here we provide evidence for opposite effects in PaT resistance of homologous recombination (HR) and non-homologous end joining (N...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2007.07.010
更新日期:2007-12-01 00:00:00
abstract::The RecQ family of DNA helicases performs essential functions in the maintenance of genomic stability in all organisms. In Deinococcus radiodurans, DR1289 is a special member of RecQ family with unique arrangement of three tandem HRDC domains in the C-terminus. A dr1289 mutant is hypersensitive to gamma-irradiation, U...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2006.09.006
更新日期:2007-02-04 00:00:00
abstract::The DNA repair protein, O(6)-alkylguanine-DNA alkyltransferase (MGMT) can confer resistance to the cancer chemotherapeutic effects of the class of DNA damaging drugs generally referred to as the O(6)-alkylating agents. Inactivation of MGMT is thus a practical approach to improving the efficacy of such agents. An accou...
journal_title:DNA repair
pub_type: 历史文章,杂志文章
doi:10.1016/j.dnarep.2007.03.015
更新日期:2007-08-01 00:00:00
abstract::The ability of the radiomimetic anti-tumor enediyne C-1027 to induce DNA inter-strand crosslinks (ICLs), in addition to the expected DNA strand breaks, is unique among traditional DNA targeted cancer therapies. Importantly, radiation therapy and most radiomimetic drugs have diminished effect in hypoxic environments du...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2014.06.001
更新日期:2014-09-01 00:00:00
abstract::Oxidative DNA damage is repaired primarily by the base excision repair (BER) pathway in a process initiated by removal of base lesions or mismatched bases by DNA glycosylases. MutY homolog (MYH, MUTYH, or Myh1) is a DNA glycosylase which excises adenine paired with the oxidative lesion 8-oxo-7,8-dihydroguanine (8-oxoG...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2014.01.001
更新日期:2014-03-01 00:00:00
abstract::Eukaryotic genomes are duplicated by a complex machinery, utilizing high fidelity replicative B-family DNA polymerases (pols) α, δ and ε. Specialized error-prone pol ζ, the fourth B-family member, is recruited when DNA synthesis by the accurate trio is impeded by replication stress or DNA damage. The damage tolerance ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2016.11.004
更新日期:2017-01-01 00:00:00
abstract::During DNA synthesis in vitro using dNTP and rNTP concentrations present in vivo, yeast replicative DNA polymerases α, δ and ɛ (Pols α, δ and ɛ) stably incorporate rNTPs into DNA. rNTPs are also incorporated during replication in vivo, and they are repaired in an RNase H2-dependent manner. In strains encoding a mutato...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2011.02.001
更新日期:2011-05-05 00:00:00
abstract::Cadmium (Cd(2+)) is a ubiquitous environmental pollutant and human carcinogen. The molecular basis of its toxicity remains unclear. Here, to identify the landscape of genes and cell functions involved in cadmium resistance, we have screened the Saccharomyces cerevisiae deletion collection for mutants sensitive to cadm...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.04.005
更新日期:2008-08-02 00:00:00
abstract::recX is a small open reading frame located downstream of recA that is conserved in many bacteria. In Escherichia coli, the recX gene (also named oraA) is a 501 bp open reading frame that encodes a predicted basic protein. Transcriptional analysis by Northern blots showed that in E. coli the recX gene is SOS-regulated....
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(02)00217-3
更新日期:2003-03-01 00:00:00
abstract::p53 Binding protein 1 (53BP1) belongs to a family of evolutionarily conserved DNA damage checkpoint proteins with C-terminal BRCT domains and is most likely the human ortholog of the budding yeast Rad9 protein, the first cell cycle checkpoint protein to be described. 53BP1 localizes rapidly to sites of DNA double stra...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2004.03.017
更新日期:2004-08-01 00:00:00
abstract::Nonhomologous end joining (NHEJ) is the major pathway for the repair of DNA double strand breaks (DSBs) in human cells. NHEJ requires the catalytic subunit of the DNA-dependent protein kinase (DNA-PKcs), Ku70, Ku80, XRCC4, DNA ligase IV and Artemis, as well as DNA polymerases mu and lambda and polynucleotide kinase. R...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.06.015
更新日期:2008-10-01 00:00:00
abstract::RecQ5 belongs to the RecQ DNA helicase family that includes genes causative of Bloom, Werner, and Rothmund-Thomson syndromes. Although no human disease has been genetically linked to a mutation in RecQ5, Drosophila melanogaster RecQ5 is highly expressed in early embryos, suggesting an important role for it in the DNA ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.10.007
更新日期:2009-02-01 00:00:00
abstract::Several types of DNA lesion are induced after ionizing irradiation (IR) of which double strand breaks (DSBs) are expected to be the most lethal, although single strand breaks (SSBs) and DNA base damages are quantitatively in the majority. Proteins of the base excision repair (BER) pathway repair these numerous lesions...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.11.008
更新日期:2009-03-01 00:00:00
abstract::Cancer-prone diseases ataxia-telangiectasia (AT), Nijmegen breakage syndrome (NBS) and ataxia-telangiectasia-like disorder (ATLD) are defective in the repair of DNA double-stranded break (DSB). On the other hand, arsenic (As) has been reported to cause DSB and to be involved in the occurrence of skin, lung and bladder...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(01)00009-x
更新日期:2002-02-28 00:00:00
abstract::In all organisms studied to date, 8-oxoguanine (GO), an important oxidation product of guanine, is removed by highly conserved GO DNA glycosylases. The hyperthermophilic crenarchaeon Pyrobaculum aerophilum encodes a GO DNA glycosylase, Pa-AGOG (Archaeal GO DNA glycosylase) which has become the founding member of a new...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2009.03.009
更新日期:2009-07-04 00:00:00
abstract::In both replicating and non-replicating cells, the maintenance of genomic stability is of utmost importance. Dividing cells can repair DNA damage during cell division, tolerate the damage by employing potentially mutagenic DNA polymerases or die via apoptosis. However, the options for accurate DNA repair are more limi...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2019.102669
更新日期:2019-09-01 00:00:00
abstract::Although correlative studies demonstrate a reduction in the expression of apurinic/apyrimidinic endonuclease/redox effector factor (Ape1/Ref-1 or Ape1) in neural tissues after neuronal insult, the role of Ape1 in regulating neurotoxicity remains to be elucidated. To address this issue, we examined the effects of reduc...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2004.11.006
更新日期:2005-03-02 00:00:00
abstract::Nucleotide excision repair (NER) stands out among other DNA repair systems for its ability to process a diverse set of unrelated DNA lesions. In bacteria, NER damage detection is orchestrated by the UvrA and UvrB proteins, which form the UvrA2-UvrB2 (UvrAB) damage sensing complex. The highly versatile damage recogniti...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2020.103024
更新日期:2021-01-01 00:00:00
abstract::Bacterial MutS2 proteins, consisting of functional domains for ATPase, DNA-binding, and nuclease activities, play roles in DNA recombination and repair. Here we observe a mechanism for generating MutS2 expression diversity in the human pathogen Helicobacter pylori, and identify a unique MutS2 domain responsible for sp...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2017.07.004
更新日期:2017-09-01 00:00:00
abstract::ATM and ATR are stress-response kinases which respond to a variety of insults including ionizing radiation, replication arrest, ultraviolet radiation and hypoxia/re-oxygenation. Hypoxia occupies a unique niche in the study of both ATR- and ATM-mediated checkpoint pathways. Hypoxia is a physiologically significant stre...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2004.03.035
更新日期:2004-08-01 00:00:00
abstract::Base excision repair (BER) of damaged or inappropriate bases in DNA has been reported to take place by single nucleotide insertion or through incorporation of several nucleotides, termed short-patch and long-patch repair, respectively. We found that extracts from proliferating and non-proliferating cells both had capa...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2009.04.002
更新日期:2009-07-04 00:00:00
abstract::Thymidylate deprivation brings about "thymineless death" in prokaryotes and eukaryotes. Although the precise mechanism for thymineless death has remained elusive, inhibition of the enzyme thymidylate synthase (TS), which catalyzes the de novo synthesis of TMP, has served for many years as a basis for chemotherapeutic ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.06.006
更新日期:2008-10-01 00:00:00
abstract::By combining mutations in DNA repair genes, important and unexpected interactions between different repair pathways can be discovered. In this study, we identified a novel link between mismatch repair (MMR) genes and postreplication repair (PRR) in Saccharomyces cerevisiae. Strains lacking Rad5 (HLTF in mammals), a pr...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2020.102870
更新日期:2020-01-01 00:00:00
abstract::Brh2, the BRCA2 ortholog in the fungus Ustilago maydis, harbors two different DNA-binding domains, one located in the N-terminal region and the other located in the C-terminal region. Here we were interested in comparing the biochemical properties of Brh2 fragments, Brh2(NT) and Brh2(CT), respectively, harboring the t...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2014.07.013
更新日期:2014-10-01 00:00:00
abstract::Accumulation of 8-oxo-7,8-dihydroguanine (8-oxoG) in the DNA results in genetic instability and mutagenesis, and is believed to contribute to carcinogenesis, aging processes and various aging-related diseases. 8-OxoG is removed from the DNA via DNA base excision repair (BER), initiated by 8-oxoguanine DNA glycosylase-...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2013.06.006
更新日期:2013-10-01 00:00:00
abstract::Maintenance of the mitochondrial genome (mtDNA) is essential for proper cellular function. The accumulation of damage and mutations in the mtDNA leads to diseases, cancer, and aging. Mammalian mitochondria have proficient base excision repair, but the existence of other DNA repair pathways is still unclear. Deficienci...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2009.01.021
更新日期:2009-06-04 00:00:00
abstract::We have identified two fission yeast homologs of budding yeast Rad4 and human xeroderma pigmentosum complementation group C (XP-C) correcting protein, designated Rhp4A and Rhp4B. Here we show that the rhp4 genes encode NER factors that are required for UV-induced DNA damage repair in fission yeast. The rhp4A-deficient...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(02)00108-8
更新日期:2002-10-01 00:00:00
abstract::Cells carrying deletions of genes encoding H-class ribonucleases display elevated rates of chromosome instability. The role of these enzymes is to remove RNA-DNA associations including persistent mRNA-DNA hybrids (R-loops) formed during transcription, and ribonucleotides incorporated into DNA during replication. RNase...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2017.02.012
更新日期:2017-04-01 00:00:00
abstract::Single-strand-dependent DNA exonucleases play important roles in DNA repair and recombination in all organisms. In Escherichia coli the redundant functions provided by the RecJ, ExoI, ExoVII and ExoX exonucleases are required for mismatch repair, UV resistance and homologous recombination. We have examined whether the...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(03)00135-6
更新日期:2003-11-21 00:00:00