Abstract:
:RecQ5 belongs to the RecQ DNA helicase family that includes genes causative of Bloom, Werner, and Rothmund-Thomson syndromes. Although no human disease has been genetically linked to a mutation in RecQ5, Drosophila melanogaster RecQ5 is highly expressed in early embryos, suggesting an important role for it in the DNA metabolism of the early embryo. In this present study, we generated RecQ5 mutants in D. melanogaster. Embryos lacking maternally derived RecQ5 contained irregular nuclei in early embryogenesis. These irregular nuclei emerged in nuclear cycle 11-13, lost cell-cycle markers, and were located below the surface monolayer of nuclei. By time-lapse microscopy, these irregular nuclei were observed not to divide, whereas all neighboring nuclei proceeded through normal mitotic division with synchrony. These data suggest that the irregular nuclei exited from the nuclear division cycle. This phenotype is reminiscent of the effect of X-ray irradiation on wild-type embryos and was rescued by expression of RecQ5. Thus, the maternal supply of RecQ5 is important for the nuclear cycles in syncytical embryos. Furthermore, the frequencies of spontaneous and induced chromosomal aberrations were increased in RecQ5 mutant neuroblasts. These data imply that DNA damage accumulates spontaneously in RecQ5 mutants. Therefore, endogenous genomic damage may be produced in Drosophila development, and RecQ5 would be involved in the maintenance of genomic stability by suppressing the accumulation of DNA damage.
journal_name
DNA Repair (Amst)journal_title
DNA repairauthors
Nakayama M,Yamaguchi S,Sagisu Y,Sakurai H,Ito F,Kawasaki Kdoi
10.1016/j.dnarep.2008.10.007subject
Has Abstractpub_date
2009-02-01 00:00:00pages
232-41issue
2eissn
1568-7864issn
1568-7856pii
S1568-7864(08)00368-6journal_volume
8pub_type
杂志文章相关文献
DNA REPAIR文献大全abstract::Base excision repair (BER) is a major defense pathway against spontaneous DNA damage. This multistep process is initiated by DNA glycosylases that recognise and excise the damaged base, and proceeds by the concerted action of additional proteins that perform incision of the abasic site, gap filling and ligation. BER h...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2018.02.011
更新日期:2018-05-01 00:00:00
abstract::The proximity of the mitochondrial genome to the respiratory chain, a major source of ROS (radical oxygen species), makes mtDNA more vulnerable to oxidative damage than nuclear DNA. Mitochondrial BER (base excision repair) is generally considered to be the main pathway involved in the prevention of oxidative lesion-in...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.11.004
更新日期:2009-03-01 00:00:00
abstract::Exposure to ionizing radiation results in a variety of genome rearrangements that have been linked to tumor formation. Many of these rearrangements are thought to arise from the repair of double-strand breaks (DSBs) by several mechanisms, including homologous recombination (HR) between repetitive sequences dispersed t...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.02.003
更新日期:2008-05-03 00:00:00
abstract::Expansion of a CGG-repeat tract in the 5' UTR of FMR1 is responsible for the Fragile X-related disorders (FXDs), FXTAS, FXPOI and FXS. Previous work in a mouse model of these disorders has implicated proteins in the base excision and the mismatch repair (MMR) pathways in the expansion mechanism. However, the precise r...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2018.12.004
更新日期:2019-02-01 00:00:00
abstract::Pyridine nucleotides are essential coenzymes in many cellular redox reactions in all living systems. In addition to functioning as a redox carrier, NAD(+) is also a required co-substrate for the conserved sirtuin deacetylases. Sirtuins regulate transcription, genome maintenance and metabolism and function as molecular...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2014.07.009
更新日期:2014-11-01 00:00:00
abstract::The accuracy of DNA synthesis depends on the accuracy of the polymerase as well as the quality and concentration(s) of the available 5'-deoxynucleoside-triphosphate DNA precursors (dNTPs). The relationships between dNTPs and error rates have been studied in vitro, but only limited insights exist into these correlation...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2012.10.011
更新日期:2013-01-01 00:00:00
abstract::The Escherichia coli orf135 gene encodes a 15.4kDa protein with homology to the MutT family of nucleotide hydrolases. The orf135 gene was cloned within a glutathione S-transferase (GST) fusion protein expression vector, which was used to overproduce the GST-Orf135 fusion protein in E. coli. The fusion protein thus obt...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(02)00057-5
更新日期:2002-07-17 00:00:00
abstract::Artemis and PALF (also called APLF) appear to be among the primary nucleases involved in non-homologous end joining (NHEJ) and responsible for most nucleolytic end processing in NHEJ. About 60% of NHEJ events show an alignment of the DNA ends that use 1 or 2bp of microhomology (MH) between the two DNA termini. Thus, M...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2014.02.006
更新日期:2014-05-01 00:00:00
abstract::Proteins that act on DNA, or are in close proximity to it, can become inadvertently crosslinked to DNA and form highly toxic lesions, known as DNA-protein crosslinks (DPCs). DPCs are generated by different chemotherapeutics, environmental or endogenous sources of crosslinking agents, or by lesions on DNA that stall th...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2020.102924
更新日期:2020-10-01 00:00:00
abstract::PriA, a 3'-->5' superfamily 2 DNA helicase, acts to remodel stalled replication forks and as a specificity factor for origin-independent assembly of a new replisome at the stalled fork. The ability of PriA to initiate replication at stalled forked structures ensures complete genome replication and helps to protect the...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2009.12.009
更新日期:2010-03-02 00:00:00
abstract::Detection of γ-H2AX foci as a measure of DNA double strand break induction and repair provides the basis of a rapid approach to establish individual radiosensitivity. However, the assignment of criteria to define increased radiosensitivity is not straightforward. Experimental end points, analytical methods and prolife...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2013.07.002
更新日期:2013-10-01 00:00:00
abstract::Lagging strand DNA replication requires the concerted actions of DNA polymerase δ, Fen1 and DNA ligase I for the removal of the RNA/DNA primers before ligation of Okazaki fragments. To better understand this process in human cells, we have reconstituted Okazaki fragment processing by the short flap pathway in vitro wi...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2013.08.008
更新日期:2013-11-01 00:00:00
abstract::The Saccharomyces cerevisiae heterotrimeric checkpoint clamp consisting of the Rad17, Mec3, and Ddc1 subunits (Rad17/3/1, the 9-1-1 complex in humans) is an early response factor to DNA damage in a signal transduction pathway leading to the activation of the checkpoint system and eventually to cell cycle arrest. These...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2005.07.008
更新日期:2005-09-28 00:00:00
abstract::Before a deleterious DNA lesion can be replaced with its undamaged counterpart, the lesion must first be removed from the genome. This process of removing and replacing DNA lesions is accomplished by the careful coordination of several protein factors during DNA repair. One such factor is the multifunctional enzyme hu...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2018.08.012
更新日期:2018-11-01 00:00:00
abstract::Radiation-induced complex double-strand breaks (DSBs) characterised by base lesions, abasic sites or single-strand breaks in close proximity to the break termini, are believed to be a major cause of the biological effects of ionising radiation exposure. It has been hypothesised that complex DSBs pose problems for the ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.05.001
更新日期:2008-08-02 00:00:00
abstract::Deinococcus radiodurans recovering from the effect of acute dose of gamma (gamma) radiation shows a biphasic mechanism of DNA double strands breaks repair that involves an efficient homologous recombination. However, it shows higher sensitivity to near-UV (NUV) than Escherichia coli and lacks RecBC, a DNA strand break...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2007.07.007
更新日期:2008-01-01 00:00:00
abstract::Analysis of the spectrum of UV-induced mutations generated in synchronized wild-type S-phase cells reveals that only approximately 25% of mutations occur at thymine (T), whilst 75% are targeted to cytosine (C). The mutational spectra changes dramatically in XP-V cells, devoid of poleta, where approximately 45% of muta...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2005.09.011
更新日期:2006-02-03 00:00:00
abstract::The XPB DNA helicase, a subunit of the basal transcription factor TFIIH, is also involved in nucleotide excision repair (NER). We examined recruitment of NER proteins in XP-B cells from patients with mild or severe xeroderma pigmentosum (XP) having different XPB mutations using local UV-irradiation through filters wit...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2007.03.025
更新日期:2007-09-01 00:00:00
abstract::3-Nitrobenzanthrone (3-NBA) is a highly mutagenic compound and possible human carcinogen found in diesel exhaust. 3-NBA forms bulky DNA adducts following metabolic activation and induces predominantly G:CT:A transversions in a variety of experimental systems. Here we investigated the influence of nucleotide excision r...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2015.11.004
更新日期:2016-03-01 00:00:00
abstract::Cancer-prone diseases ataxia-telangiectasia (AT), Nijmegen breakage syndrome (NBS) and ataxia-telangiectasia-like disorder (ATLD) are defective in the repair of DNA double-stranded break (DSB). On the other hand, arsenic (As) has been reported to cause DSB and to be involved in the occurrence of skin, lung and bladder...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(01)00009-x
更新日期:2002-02-28 00:00:00
abstract::Ubiquitin conjugation plays critical roles in virtually all DNA repair pathways. This review provides an overview of the known multi-domain RING/Ubox E3 ligases and their domain structures. An analysis of known RING/Ubox X-ray and NMR structures leads to a discussion of the effects of dimerization. Structural and mech...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2009.01.014
更新日期:2009-04-05 00:00:00
abstract::The mammalian CtIP protein and its orthologs in other eukaryotes promote the resection of DNA double-strand breaks and are essential for meiotic recombination. Here we review the current literature supporting the role of CtIP in DNA end processing and the importance of CtIP endonuclease activity in DNA repair. We also...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2015.04.016
更新日期:2015-08-01 00:00:00
abstract::Despite detailed knowledge on the genetic network and biochemical properties of most of the nucleotide excision repair (NER) proteins, cell biological analysis has only recently made it possible to investigate the temporal and spatial organization of NER. In contrast to several other DNA damage response mechanisms tha...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2011.04.015
更新日期:2011-07-15 00:00:00
abstract::A recently discovered group of novel polymerases are characterized by significantly reduced fidelity of DNA synthesis in vitro. This feature is consistent with the relaxed fidelity required for the replicative bypass of various types of base damage that frequently block high fidelity replicative polymerases. The prese...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(02)00189-1
更新日期:2003-01-02 00:00:00
abstract::The nonhomologous DNA end-joining pathway (NHEJ), a major pathway for repairing DNA double-strand breaks (DSBs), is essential for maintaining genomic stability. Knockout animals for components in this pathway demonstrate a distinct pattern of cell death in the developing brain. Here we demonstrate that cell death is a...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2003.08.011
更新日期:2003-12-09 00:00:00
abstract::RECQ proteins are conserved DNA helicases in both prokaryotes and eukaryotes. The importance of the RECQ family helicases in human health is demonstrated by their roles as cancer suppressors that are vital for preserving genome integrity. Mutations in one of the RECQ family proteins, RECQ4, not only result in developm...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2010.01.006
更新日期:2010-03-02 00:00:00
abstract::Flaws in the DNA replication process have emerged as a leading driver of genome instability in human diseases. Alteration to replication fork progression is a defining feature of replication stress and the consequent failure to maintain fork integrity and complete genome duplication within a single round of S-phase co...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2018.08.017
更新日期:2018-11-01 00:00:00
abstract::In all organisms studied to date, 8-oxoguanine (GO), an important oxidation product of guanine, is removed by highly conserved GO DNA glycosylases. The hyperthermophilic crenarchaeon Pyrobaculum aerophilum encodes a GO DNA glycosylase, Pa-AGOG (Archaeal GO DNA glycosylase) which has become the founding member of a new...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2009.03.009
更新日期:2009-07-04 00:00:00
abstract::We have used the recently determined crystal structures of Escherichia coli (E. coli) MutS, MutL and MutH to guide construction of 47 amino-acid substitutions in these proteins and analyzed their behavior in mismatch repair and recombination in vitro and in vivo. We find that the active site of the MutH endonuclease i...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(02)00245-8
更新日期:2003-04-02 00:00:00
abstract::Maintenance of DNA integrity is vital for all of the living organisms. Consequence of DNA damaging ranges from, introducing harmless synonymous mutations, to causing disease-associated mutations, genome instability, and cell death. A cell cycle protein cyclin D1 is an established cancer-driving protein. However, contr...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2016.04.011
更新日期:2016-06-01 00:00:00