当前位置： SCI文献检索 > DNA REPAIR期刊下所有文献 > Non-homologous end joining often uses microhomology: implications for alternative end joining.

Non-homologous end joining often uses microhomology: implications for alternative end joining.

Abstract：

:Artemis and PALF (also called APLF) appear to be among the primary nucleases involved in non-homologous end joining (NHEJ) and responsible for most nucleolytic end processing in NHEJ. About 60% of NHEJ events show an alignment of the DNA ends that use 1 or 2bp of microhomology (MH) between the two DNA termini. Thus, MH is a common feature of NHEJ. For most naturally occurring human chromosomal deletions (e.g., after oxidative damage or radiation) and translocations, such as those seen in human neoplasms and as well as inherited chromosomal structural variations, MH usage occurs at a frequency that is typical of NHEJ, and does not suggest major involvement of alternative pathways that require more extensive MH. Though we mainly focus on human NHEJ at double-strand breaks, comparison on these points to other eukaryotes, primarily S. cerevisiae, is informative.

journal_name

DNA Repair (Amst)

journal_title

DNA repair

authors

Pannunzio NR,Li S,Watanabe G,Lieber MR

doi

10.1016/j.dnarep.2014.02.006

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

74-80

eissn

1568-7864

issn

1568-7856

pii

S1568-7864(14)00043-3

journal_volume

17

pub_type

杂志文章,评审

相关文献

DNA REPAIR文献大全
  • Repair of radiation induced DNA double strand breaks by backup NHEJ is enhanced in G2.

    abstract：:In higher eukaryotes DNA double strand breaks (DSBs) are repaired by homologous recombination (HRR) or non-homologous end joining (NHEJ). In addition to the DNA-PK dependent pathway of NHEJ (D-NHEJ), cells employ a backup pathway (B-NHEJ) utilizing Ligase III and PARP-1. The cell cycle dependence and coordination of t...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2007.11.008

    authors： Wu W,Wang M,Wu W,Singh SK,Mussfeldt T,Iliakis G

    更新日期：2008-02-01 00:00:00

  • Mismatch repair-independent tandem repeat sequence instability resulting from ribonucleotide incorporation by DNA polymerase ε.

    abstract：:During DNA synthesis in vitro using dNTP and rNTP concentrations present in vivo, yeast replicative DNA polymerases α, δ and ɛ (Pols α, δ and ɛ) stably incorporate rNTPs into DNA. rNTPs are also incorporated during replication in vivo, and they are repaired in an RNase H2-dependent manner. In strains encoding a mutato...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2011.02.001

    authors： Clark AB,Lujan SA,Kissling GE,Kunkel TA

    更新日期：2011-05-05 00:00:00

  • Intrinsic mitochondrial DNA repair defects in Ataxia Telangiectasia.

    abstract：:Ataxia Telangiectasia (A-T) is a progressive childhood disorder characterized most notably by cerebellar degeneration and predisposition to cancer. A-T is caused by mutations in the kinase ATM, a master regulator of the DNA double-strand break response. In addition to DNA-damage signaling defects, A-T cells display mi...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2013.11.002

    authors： Sharma NK,Lebedeva M,Thomas T,Kovalenko OA,Stumpf JD,Shadel GS,Santos JH

    更新日期：2014-01-01 00:00:00

  • The splicing component ISY1 regulates APE1 in base excision repair.

    abstract：:The integrity of cellular genome is continuously challenged by endogenous and exogenous DNA damaging agents. If DNA damage is not removed in a timely fashion the replisome may stall at DNA lesions, causing fork collapse and genetic instability. Base excision DNA repair (BER) is the most important pathway for the remov...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2019.102769

    authors： Jaiswal AS,Williamson EA,Srinivasan G,Kong K,Lomelino CL,McKenna R,Walter C,Sung P,Narayan S,Hromas R

    更新日期：2020-02-01 00:00:00

  • Disruption of SUMO-targeted ubiquitin ligases Slx5-Slx8/RNF4 alters RecQ-like helicase Sgs1/BLM localization in yeast and human cells.

    abstract：:RecQ-like helicases are a highly conserved protein family that functions during DNA repair and, when mutated in humans, is associated with cancer and/or premature aging syndromes. The budding yeast RecQ-like helicase Sgs1 has important functions in double-strand break (DSB) repair of exogenously induced breaks, as wel...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2014.12.004

    authors： Böhm S,Mihalevic MJ,Casal MA,Bernstein KA

    更新日期：2015-02-01 00:00:00

  • Measurement of DNA base and nucleotide excision repair activities in mammalian cells and tissues using the comet assay--a methodological overview.

    abstract：:There is an increasing demand for phenotyping assays in the field of human functional genetics. DNA repair activity is representative of this functional approach, being seen as a valuable biomarker related to cancer risk. Repair activity is evaluated by incubating a cell extract with a DNA substrate containing lesions...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2013.07.011

    authors： Azqueta A,Langie SA,Slyskova J,Collins AR

    更新日期：2013-11-01 00:00:00

  • One ring to bring them all--the role of Ku in mammalian non-homologous end joining.

    abstract：:The repair of DNA double strand breaks is essential for cell survival and several conserved pathways have evolved to ensure their rapid and efficient repair. The non-homologous end joining pathway is initiated when Ku binds to the DNA break site. Ku is an abundant nuclear heterodimer of Ku70 and Ku80 with a toroidal s...

    journal_title：DNA repair

    pub_type： 杂志文章,评审

    doi：10.1016/j.dnarep.2014.02.019

    authors： Grundy GJ,Moulding HA,Caldecott KW,Rulten SL

    更新日期：2014-05-01 00:00:00

  • High levels of oxidatively generated DNA damage 8,5'-cyclo-2'-deoxyadenosine accumulate in the brain tissues of xeroderma pigmentosum group A gene-knockout mice.

    abstract：:Xeroderma pigmentosum (XP) is a genetic disorder associated with defects in nucleotide excision repair, a pathway that eliminates a wide variety of helix-distorting DNA lesions, including ultraviolet-induced pyrimidine dimers. In addition to skin diseases in sun-exposed areas, approximately 25% of XP patients develop ...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2019.04.004

    authors： Mori T,Nakane H,Iwamoto T,Krokidis MG,Chatgilialoglu C,Tanaka K,Kaidoh T,Hasegawa M,Sugiura S

    更新日期：2019-08-01 00:00:00

  • Homologous recombination and the yKu70/80 complex exert opposite roles in resistance against the killer toxin from Pichia acaciae.

    abstract：:The linear plasmid (pPac1-2) encoded killer toxin (PaT) of the yeast Pichia acaciae arrests sensitive Saccharomyces cerevisiae cells in the S-phase of the cell cycle and induces mutations. Here we provide evidence for opposite effects in PaT resistance of homologous recombination (HR) and non-homologous end joining (N...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2007.07.010

    authors： Klassen R,Krampe S,Meinhardt F

    更新日期：2007-12-01 00:00:00

  • Conserved helicase domain of human RecQ4 is required for strand annealing-independent DNA unwinding.

    abstract：:Humans have five members of the well conserved RecQ helicase family: RecQ1, Bloom syndrome protein (BLM), Werner syndrome protein (WRN), RecQ4, and RecQ5, which are all known for their roles in maintaining genome stability. BLM, WRN, and RecQ4 are associated with premature aging and cancer predisposition. Of the three...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2010.04.003

    authors： Rossi ML,Ghosh AK,Kulikowicz T,Croteau DL,Bohr VA

    更新日期：2010-07-01 00:00:00

  • The endonuclease domain of Bacillus subtilis MutL is functionally asymmetric.

    abstract：:DNA mismatch repair is an evolutionarily conserved repair pathway that corrects replication errors. In most prokaryotes and all eukaryotes, the mismatch repair protein MutL is a sequence-unspecific endonuclease that nicks the newly synthesized strand and marks it for repair. Although the sequence of the endonuclease d...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2018.10.003

    authors： Liu L,Ortiz Castro MC,Rodríguez González J,Pillon MC,Guarné A

    更新日期：2019-01-01 00:00:00

  • Human AP-endonuclease (Ape1) activity on telomeric G4 structures is modulated by acetylatable lysine residues in the N-terminal sequence.

    abstract：:Loss of telomeres stability is a hallmark of cancer cells. Exposed telomeres are prone to aberrant end-joining reactions leading to chromosomal fusions and translocations. Human telomeres contain repeated TTAGGG elements, in which the 3' exposed strand may adopt a G-quadruplex (G4) structure. The guanine-rich regions ...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2018.11.010

    authors： Burra S,Marasco D,Malfatti MC,Antoniali G,Virgilio A,Esposito V,Demple B,Galeone A,Tell G

    更新日期：2019-01-01 00:00:00

  • Archaeal DNA uracil repair via direct strand incision: A minimal system reconstituted from purified components.

    abstract：:Hydrolytic deamination of DNA cytosine residues results in U/G mispairs, pre-mutagenic lesions threatening long-term genetic stability. Hence, DNA uracil repair is ubiquitous throughout all extant life forms and base excision repair, triggered by a uracil DNA glycosylase (UDG), is the mechanistic paradigm adopted, as ...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2010.01.004

    authors： Schomacher L,Schürer KA,Ciirdaeva E,McDermott P,Chong JP,Kramer W,Fritz HJ

    更新日期：2010-04-04 00:00:00

  • Base excision repair and nucleotide excision repair contribute to the removal of N-methylpurines from active genes.

    abstract：:Many different cellular pathways have evolved to protect the genome from the deleterious effects of DNA damage that result from exposure to chemical and physical agents. Among these is a process called transcription-coupled repair (TCR) that catalyzes the removal of DNA lesions from the transcribed strand of expressed...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/s1568-7864(02)00075-7

    authors： Plosky B,Samson L,Engelward BP,Gold B,Schlaen B,Millas T,Magnotti M,Schor J,Scicchitano DA

    更新日期：2002-08-06 00:00:00

  • The hyper unequal sister chromatid recombination in an sgs1 mutant of budding yeast requires MSH2.

    abstract：:Budding yeast SGS1 and the human Bloom's syndrome (BS) gene, BLM, are homologues of the Escherichia coli recQ. Cells derived from BS patients are characterized by a dramatic increase in sister chromatid exchange (SCE). We previously reported that budding yeast cells deficient in SGS1 showed an increase in the frequenc...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2004.05.008

    authors： Onoda F,Seki M,Wang W,Enomoto T

    更新日期：2004-10-05 00:00:00

  • RAD59 is required for efficient repair of simultaneous double-strand breaks resulting in translocations in Saccharomyces cerevisiae.

    abstract：:Exposure to ionizing radiation results in a variety of genome rearrangements that have been linked to tumor formation. Many of these rearrangements are thought to arise from the repair of double-strand breaks (DSBs) by several mechanisms, including homologous recombination (HR) between repetitive sequences dispersed t...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2008.02.003

    authors： Pannunzio NR,Manthey GM,Bailis AM

    更新日期：2008-05-03 00:00:00

  • An improved method for the detection of nucleotide excision repair factors at local UV DNA damage sites.

    abstract：:Among different DNA repair processes that cells use to face with DNA damage, nucleotide excision repair (NER) is particularly important for the removal of a high variety of lesions, including those generated by some antitumor drugs. A number of factors participating in NER, such as the TFIIH complex and the endonuclea...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2017.01.005

    authors： Dutto I,Cazzalini O,Stivala LA,Prosperi E

    更新日期：2017-03-01 00:00:00

  • Bacillus subtilis DisA helps to circumvent replicative stress during spore revival.

    abstract：:The mechanisms that allow to circumvent replicative stress, and to resume DNA synthesis are poorly understood in Bacillus subtilis. To study the role of the diadenylate cyclase DisA and branch migration translocase (BMT) RadA/Sms in restarting a stalled replication fork, we nicked and broke the circular chromosome of ...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2017.09.006

    authors： Raguse M,Torres R,Seco EM,Gándara C,Ayora S,Moeller R,Alonso JC

    更新日期：2017-11-01 00:00:00

  • Impaired spermatogenesis and elevated spontaneous tumorigenesis in xeroderma pigmentosum group A gene (Xpa)-deficient mice.

    abstract：:We have reported that xeroderma pigmentosum group A (Xpa) gene-knockout mice [Xpa (-/-) mice] are deficient in nucleotide excision repair (NER) and highly sensitive to UV-induced skin carcinogenesis. Although xeroderma pigmentosum group A patients show growth retardation, immature sexual development, and neurological ...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2008.08.003

    authors： Nakane H,Hirota S,Brooks PJ,Nakabeppu Y,Nakatsu Y,Nishimune Y,Iino A,Tanaka K

    更新日期：2008-12-01 00:00:00

  • In vitro and in vivo studies of MutS, MutL and MutH mutants: correlation of mismatch repair and DNA recombination.

    abstract：:We have used the recently determined crystal structures of Escherichia coli (E. coli) MutS, MutL and MutH to guide construction of 47 amino-acid substitutions in these proteins and analyzed their behavior in mismatch repair and recombination in vitro and in vivo. We find that the active site of the MutH endonuclease i...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/s1568-7864(02)00245-8

    authors： Junop MS,Yang W,Funchain P,Clendenin W,Miller JH

    更新日期：2003-04-02 00:00:00

  • recX, a new SOS gene that is co-transcribed with the recA gene in Escherichia coli.

    abstract：:recX is a small open reading frame located downstream of recA that is conserved in many bacteria. In Escherichia coli, the recX gene (also named oraA) is a 501 bp open reading frame that encodes a predicted basic protein. Transcriptional analysis by Northern blots showed that in E. coli the recX gene is SOS-regulated....

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/s1568-7864(02)00217-3

    authors： Pagès V,Koffel-Schwartz N,Fuchs RP

    更新日期：2003-03-01 00:00:00

  • DNA damage response and breast cancer development: Possible therapeutic applications of ATR, ATM, PARP, BRCA1 inhibition.

    abstract：:Breast cancer is the most common and significant cancers in females regarding the loss of life quality. Similar to other cancers, one of the etiologic factors in breast cancer is DNA damage. A plethora of molecules are responsible for sensing DNA damage and mediating actions which lead to DNA repair, senescence, cell ...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2020.103032

    authors： Mirza-Aghazadeh-Attari M,Recio MJ,Darband SG,Kaviani M,Safa A,Mihanfar A,Sadighparvar S,Karimian A,Alemi F,Majidinia M,Yousefi B

    更新日期：2020-12-17 00:00:00

  • Loss of RecQ5 leads to spontaneous mitotic defects and chromosomal aberrations in Drosophila melanogaster.

    abstract：:RecQ5 belongs to the RecQ DNA helicase family that includes genes causative of Bloom, Werner, and Rothmund-Thomson syndromes. Although no human disease has been genetically linked to a mutation in RecQ5, Drosophila melanogaster RecQ5 is highly expressed in early embryos, suggesting an important role for it in the DNA ...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2008.10.007

    authors： Nakayama M,Yamaguchi S,Sagisu Y,Sakurai H,Ito F,Kawasaki K

    更新日期：2009-02-01 00:00:00

  • Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the preferred targets of the ATM family of protein kinases do not affect DNA repair or telomere length in Saccharomyces cerevisiae.

    abstract：:In eukaryotes, mutations in a number of genes that affect DNA damage checkpoints or DNA replication also affect telomere length [Curr. Opin. Cell Biol. 13 (2001) 281]. Saccharomyces cerevisae strains with mutations in the TEL1 gene (encoding an ATM-like protein kinase) have very short telomeres, as do strains with mut...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/s1568-7864(03)00115-0

    authors： Mallory JC,Bashkirov VI,Trujillo KM,Solinger JA,Dominska M,Sung P,Heyer WD,Petes TD

    更新日期：2003-09-18 00:00:00

  • The mechanism of human tyrosyl-DNA phosphodiesterase 1 in the cleavage of AP site and its synthetic analogs.

    abstract：:The mechanism of hydrolysis of the apurinic/apyrimidinic (AP) site and its synthetic analogs by using tyrosyl-DNA phosphodiesterase 1 (Tdp1) was analyzed. Tdp1 catalyzes the cleavage of AP site and the synthetic analog of the AP site, 3-hydroxy-2(hydroxymethyl)-tetrahydrofuran (THF), in DNA by hydrolysis of the phosph...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2013.09.008

    authors： Lebedeva NA,Rechkunova NI,Ishchenko AA,Saparbaev M,Lavrik OI

    更新日期：2013-12-01 00:00:00

  • Defects in recombination activity caused by somatic and germline mutations in the multimerization/BRCA2 binding region of human RAD51 protein.

    abstract：:The human RAD51 recombinase possesses DNA pairing and strand exchange activities that are essential for the error-free, homology-directed repair of DNA double-strand breaks. The recombination activities of RAD51 are activated upon its assembly into presynaptic filaments on single-stranded DNA at resected DSB ends. Def...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2017.10.008

    authors： Silva MC,Bryan KE,Morrical MD,Averill AM,Dragon J,Wiegmans AP,Morrical SW

    更新日期：2017-12-01 00:00:00

  • The involvement of key DNA repair pathways in the formation of chromosome rearrangements in embryonic stem cells.

    abstract：:It is vital that embryonic stem (ES) cells, which give rise to the diverse tissues of the mature organism, maintain genetic stability. To understand mechanisms for the prevention and causation of chromosomal instability, we have used spectral karyotyping (SKY) to analyse ES cells from wild-type and repair-gene knockou...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2005.05.005

    authors： Griffin C,Waard Hd,Deans B,Thacker J

    更新日期：2005-08-15 00:00:00

  • BACH2: a marker of DNA damage and ageing.

    abstract：:DNA damage and ageing share expression changes involving alterations in many aspects of metabolism, suppression of growth and upregulation of defence and genome maintenance systems. "Omics" technologies have permitted large-scale parallel measurements covering global cellular constituents and aided the identification ...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2013.08.016

    authors： Uittenboogaard LM,Payan-Gomez C,Pothof J,van Ijcken W,Mastroberardino PG,van der Pluijm I,Hoeijmakers JH,Tresini M

    更新日期：2013-11-01 00:00:00

  • Impact of DNA lesion repair, replication and formation on the mutational spectra of environmental carcinogens: Aflatoxin B1 as a case study.

    abstract：:In a multicellular organism, somatic mutations represent a permanent record of the past chemical and biochemical perturbations experienced by a cell in its local microenvironment. Akin to a perpetual recording device, with every replication, genomic DNA accumulates mutations in patterns that reflect: i) the sequence c...

    journal_title：DNA repair

    pub_type： 杂志文章,评审

    doi：10.1016/j.dnarep.2018.08.008

    authors： Fedeles BI,Essigmann JM

    更新日期：2018-11-01 00:00:00

  • Nucleolytic processing of a protein-bound DNA end by the E. coli SbcCD (MR) complex.

    abstract：:SbcCD and other Mre11/Rad50 (MR) complexes are implicated in the metabolism of DNA ends. They cleave ends sealed by hairpin structures and have been postulated to play roles in removing protein bound to DNA termini. Here we provide direct evidence that the Escherichia coli MR complex (SbcCD) removes protein from a pro...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/s1568-7864(03)00063-6

    authors： Connelly JC,de Leau ES,Leach DR

    更新日期：2003-07-16 00:00:00