Abstract:
:The mechanism of hydrolysis of the apurinic/apyrimidinic (AP) site and its synthetic analogs by using tyrosyl-DNA phosphodiesterase 1 (Tdp1) was analyzed. Tdp1 catalyzes the cleavage of AP site and the synthetic analog of the AP site, 3-hydroxy-2(hydroxymethyl)-tetrahydrofuran (THF), in DNA by hydrolysis of the phosphodiester bond between the substituent and 5' adjacent phosphate. The product of Tdp1 cleavage in the case of the AP site is unstable and is hydrolyzed with the formation of 3'- and 5'-margin phosphates. The following repair demands the ordered action of polynucleotide kinase phosphorylase, with XRCC1, DNA polymerase β, and DNA ligase. In the case of THF, Tdp1 generates break with the 5'-THF and the 3'-phosphate termini. Tdp1 is also able to effectively cleave non-nucleotide insertions in DNA, decanediol and diethyleneglycol moieties by the same mechanism as in the case of THF cleavage. The efficiency of Tdp1 catalyzed hydrolysis of AP-site analog correlates with the DNA helix distortion induced by the substituent. The following repair of 5'-THF and other AP-site analogs can be processed by the long-patch base excision repair pathway.
journal_name
DNA Repair (Amst)journal_title
DNA repairauthors
Lebedeva NA,Rechkunova NI,Ishchenko AA,Saparbaev M,Lavrik OIdoi
10.1016/j.dnarep.2013.09.008subject
Has Abstractpub_date
2013-12-01 00:00:00pages
1037-42issue
12eissn
1568-7864issn
1568-7856pii
S1568-7864(13)00238-3journal_volume
12pub_type
杂志文章相关文献
DNA REPAIR文献大全abstract::All organisms rely on integrated networks to repair DNA double-strand breaks (DSBs) in order to preserve the integrity of the genetic information, to re-establish replication, and to ensure proper chromosomal segregation. Genetic, cytological, biochemical and structural approaches have been used to analyze how Bacillu...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2012.12.005
更新日期:2013-03-01 00:00:00
abstract::XPC is one of the key DNA damage recognition proteins in the global genome repair route of the nucleotide excision repair (NER) pathway. Previously, we demonstrated that NER-deficient mouse models Xpa(-/-) and Xpc(-/-) exhibit a divergent spontaneous tumor spectrum and proposed that XPC might be functionally involved ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2013.08.019
更新日期:2013-12-01 00:00:00
abstract::Misalignment of a nascent strand and the use of an alternative template during DNA replication, a process termed "template-switching", can give rise to frequent mutations and genetic rearrangements. Mutational hotspots are frequently found associated with imperfect inverted repeats ("quasipalindromes" or "QPs") in man...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2017.05.007
更新日期:2017-09-01 00:00:00
abstract::RECQ proteins are conserved DNA helicases in both prokaryotes and eukaryotes. The importance of the RECQ family helicases in human health is demonstrated by their roles as cancer suppressors that are vital for preserving genome integrity. Mutations in one of the RECQ family proteins, RECQ4, not only result in developm...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2010.01.006
更新日期:2010-03-02 00:00:00
abstract::Exposure to ionizing radiation results in a variety of genome rearrangements that have been linked to tumor formation. Many of these rearrangements are thought to arise from the repair of double-strand breaks (DSBs) by several mechanisms, including homologous recombination (HR) between repetitive sequences dispersed t...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.02.003
更新日期:2008-05-03 00:00:00
abstract::Cockayne syndrome (CS) is a debilitating and complex disorder that results from inherited mutations in the CS complementation genes A and B, CSA and CSB. The links between the molecular functions of the CS genes and the complex pathophysiology of CS are as of yet poorly understood and are the subject of intense debate...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2014.09.011
更新日期:2014-12-01 00:00:00
abstract::Mitochondria are membrane-bound organelles found in eukaryotic cells where they generate energy through the respiratory chain. They contain their own genome that encodes genes critical to the mitochondrial function, but most of their protein content is synthetized from nuclear encoded genes. Damages to the mtDNA can c...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2019.102684
更新日期:2019-10-01 00:00:00
abstract::Hydrolytic deamination of DNA cytosine residues results in U/G mispairs, pre-mutagenic lesions threatening long-term genetic stability. Hence, DNA uracil repair is ubiquitous throughout all extant life forms and base excision repair, triggered by a uracil DNA glycosylase (UDG), is the mechanistic paradigm adopted, as ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2010.01.004
更新日期:2010-04-04 00:00:00
abstract::Multiple DNA polymerases participate in somatic hypermutation of immunoglobulin (Ig) genes. Mutations at A/T are largely dependent on DNA polymerase eta (POLH) whereas mutations at C/G appear to be generated by several DNA polymerases. We have previously shown that mice expressing a catalytically inactive POLQ (Polq-i...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2006.06.006
更新日期:2006-11-08 00:00:00
abstract::Short-wave ultra-violet light promotes the formation of DNA dimers between adjacent thymine bases, and if unrepaired these dimers may induce skin cancer. Living cells have a very robust repair system capable of repairing hundreds of lesions every day. Although many of the details of the dimer repair mechanism are know...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2006.04.007
更新日期:2006-07-13 00:00:00
abstract::As haematopoietic stem cell gene therapy utilizing O(6)-methylguanine-DNA-methyltransferase has reached the clinical stage, safety-related questions become increasingly important. These issues concern insertional mutagenesis of viral vectors, the acute toxicity of pre-transplant conditioning protocols and in vivo sele...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2007.03.021
更新日期:2007-08-01 00:00:00
abstract::A recently discovered group of novel polymerases are characterized by significantly reduced fidelity of DNA synthesis in vitro. This feature is consistent with the relaxed fidelity required for the replicative bypass of various types of base damage that frequently block high fidelity replicative polymerases. The prese...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(02)00189-1
更新日期:2003-01-02 00:00:00
abstract::3-Nitrobenzanthrone (3-NBA) is a highly mutagenic compound and possible human carcinogen found in diesel exhaust. 3-NBA forms bulky DNA adducts following metabolic activation and induces predominantly G:CT:A transversions in a variety of experimental systems. Here we investigated the influence of nucleotide excision r...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2015.11.004
更新日期:2016-03-01 00:00:00
abstract::In response to the threat of DNA damage, cells exhibit a dramatic and multi-factorial response spanning from transcriptional changes to protein modifications, collectively known as the DNA damage response (DDR). Here, we review the literature surrounding the transcriptional response to DNA damage. We review difference...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2019.05.002
更新日期:2019-07-01 00:00:00
abstract::Why does a constant barrage of DNA damage lead to disease in some individuals, while others remain healthy? This article surveys current work addressing the implications of inter-individual variation in DNA repair capacity for human health, and discusses the status of DNA repair assays as potential clinical tools for ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2014.03.009
更新日期:2014-07-01 00:00:00
abstract::Both UVB radiation and DNA-breaking agents were previously reported to kill Arabidopsis stem cells. We demonstrate that death induced by UVB or by ionizing radiation (IR) requires Suppressor of Gamma Response 1 (SOG1), a transcription factor already found to govern many responses to these agents in Arabidopsis. DNA-da...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2010.06.006
更新日期:2010-09-04 00:00:00
abstract::During DNA synthesis in vitro using dNTP and rNTP concentrations present in vivo, yeast replicative DNA polymerases α, δ and ɛ (Pols α, δ and ɛ) stably incorporate rNTPs into DNA. rNTPs are also incorporated during replication in vivo, and they are repaired in an RNase H2-dependent manner. In strains encoding a mutato...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2011.02.001
更新日期:2011-05-05 00:00:00
abstract::DNA double-strand breaks (DSBs) in yeast are repaired by homologous recombination (HR) and non-homologous end-joining (NHEJ). Rad51 forms nucleoprotein filaments at processed broken ends that effect strand exchange, forming heteroduplex DNA (hDNA) that gives rise to a gene conversion tract. We hypothesized that excess...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2005.03.003
更新日期:2005-06-08 00:00:00
abstract::Pyridine nucleotides are essential coenzymes in many cellular redox reactions in all living systems. In addition to functioning as a redox carrier, NAD(+) is also a required co-substrate for the conserved sirtuin deacetylases. Sirtuins regulate transcription, genome maintenance and metabolism and function as molecular...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2014.07.009
更新日期:2014-11-01 00:00:00
abstract::DNA polymerases beta (pol beta ) and eta (pol eta ) are the only two eukaryotic polymerases known to efficiently bypass cisplatin and oxaliplatin adducts in vitro. Frameshift errors are an important aspect of mutagenesis. We have compared the types of frameshifts that occur during translesion synthesis past cisplatin ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(02)00150-7
更新日期:2002-12-05 00:00:00
abstract::Radiation-induced complex double-strand breaks (DSBs) characterised by base lesions, abasic sites or single-strand breaks in close proximity to the break termini, are believed to be a major cause of the biological effects of ionising radiation exposure. It has been hypothesised that complex DSBs pose problems for the ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.05.001
更新日期:2008-08-02 00:00:00
abstract::From bacteria to humans, ancient stress responses enable organisms to contend with damage to both the genome and the proteome. These pathways have long been viewed as fundamentally separate responses. Yet recent discoveries from multiple fields have revealed surprising links between the two. Many DNA-damaging agents a...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2018.08.004
更新日期:2018-11-01 00:00:00
abstract::Transcription factor II H (TFIIH) is composed of core TFIIH and Cdk-activating kinase (CAK) complexes. Besides transcription, TFIIH also participates in nucleotide excision repair (NER), verifying DNA lesions through its helicase components XPB and XPD. The assembly state of TFIIH is known to be affected by truncation...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2012.09.003
更新日期:2012-12-01 00:00:00
abstract::DNA polymerases play a crucial role in the cell cycle due to their involvement in genome replication and repair. Understanding the reaction mechanism by which these polymerases carry out their function can provide insights into these processes. Recently, the crystal structures of human DNA polymerase lambda (Pollambda...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.07.007
更新日期:2008-11-01 00:00:00
abstract::Thymidylate deprivation brings about "thymineless death" in prokaryotes and eukaryotes. Although the precise mechanism for thymineless death has remained elusive, inhibition of the enzyme thymidylate synthase (TS), which catalyzes the de novo synthesis of TMP, has served for many years as a basis for chemotherapeutic ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.06.006
更新日期:2008-10-01 00:00:00
abstract::Methylation of the O(6)-methylguanine-DNA-methyltransferase (MGMT) promoter is associated with G:C to A:T transitions in the p53 gene in various human cancers, including lung cancer. In tumors with p53 mutation, MGMT promoter methylation is more common in advanced tumors than in early tumors. However, in tumors with w...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.04.016
更新日期:2008-08-02 00:00:00
abstract::The DNA damage checkpoint is a surveillance mechanism activated by DNA lesions and devoted to the maintenance of genome stability. It is considered as a signal transduction cascade, involving a sensing step, the activation of a set of protein kinases and the transmission and amplification of the damage signal through ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2004.06.019
更新日期:2004-12-02 00:00:00
abstract::Base excision repair (BER) of damaged or inappropriate bases in DNA has been reported to take place by single nucleotide insertion or through incorporation of several nucleotides, termed short-patch and long-patch repair, respectively. We found that extracts from proliferating and non-proliferating cells both had capa...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2009.04.002
更新日期:2009-07-04 00:00:00
abstract::Quantitating relative (32)P-band intensity in gels is desired, e.g., to study primer-extension kinetics of DNA polymerases (DNAPs). Following imaging, multiple (32)P-bands are often present in lanes. Though individual bands appear by eye to be simple and well-resolved, scanning reveals they are actually skewed-Gaussia...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2014.10.001
更新日期:2015-01-01 00:00:00
abstract::Despite detailed knowledge on the genetic network and biochemical properties of most of the nucleotide excision repair (NER) proteins, cell biological analysis has only recently made it possible to investigate the temporal and spatial organization of NER. In contrast to several other DNA damage response mechanisms tha...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2011.04.015
更新日期:2011-07-15 00:00:00