Abstract：
:All organisms rely on integrated networks to repair DNA double-strand breaks (DSBs) in order to preserve the integrity of the genetic information, to re-establish replication, and to ensure proper chromosomal segregation. Genetic, cytological, biochemical and structural approaches have been used to analyze how Bacillus subtilis senses DNA damage and responds to DSBs. RecN, which is among the first responders to DNA DSBs, promotes the ordered recruitment of repair proteins to the site of a lesion. Cells have evolved different mechanisms for efficient end processing to create a 3'-tailed duplex DNA, the substrate for RecA binding, in the repair of one- and two-ended DSBs. Strand continuity is re-established via homologous recombination (HR), utilizing an intact homologous DNA molecule as a template. In the absence of transient diploidy or of HR, however, two-ended DSBs can be directly re-ligated via error-prone non-homologous end-joining. Here we review recent findings that shed light on the early stages of DSB repair in Firmicutes.
journal_name
DNA Repair (Amst)journal_title
DNA repairauthors
Alonso JC,Cardenas PP,Sanchez H,Hejna J,Suzuki Y,Takeyasu Kdoi
10.1016/j.dnarep.2012.12.005subject
Has Abstractpub_date
2013-03-01 00:00:00pages
162-76issue
3eissn
1568-7864issn
1568-7856pii
S1568-7864(12)00308-4journal_volume
12pub_type
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