Abstract：
:AHNAK is a high molecular weight protein that is under-expressed in several radiosensitive neuroblastoma cell lines. Using immunoaffinity purification or purified proteins, we show that AHNAK interacts specifically with the DNA ligase IV-XRCC4 complex, a complex that functions in DNA non-homologous end-joining. Furthermore, AHNAK and the DNA ligase IV-XRCC4 complex co-immunoprecipitate demonstrating an in vivo interaction. This interaction is specific and is not observed with other DNA ligases nor with other components of the DNA non-homologous end-joining machinery. We characterised AHNAK as a protein that stimulates the double-stranded (DS) ligation activity of DNA ligase IV-XRCC4. We show that AHNAK has weak DNA-binding activity and forms a stable complex with the DNA ligase IV-XRCC4 complex on DNA. AHNAK is also able to link two DNA molecules to a similar extent to that previously reported for Ku. Together, these findings demonstrate new activities for AHNAK, and raise the possibility that it may function to modulate DNA non-homologous end-joining.
journal_name
DNA Repair (Amst)journal_title
DNA repairauthors
Stiff T,Shtivelman E,Jeggo P,Kysela Bdoi
10.1016/j.dnarep.2003.11.001keywords:
subject
Has Abstractpub_date
2004-03-04 00:00:00pages
245-56issue
3eissn
1568-7864issn
1568-7856pii
S1568786403002477journal_volume
3pub_type
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