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Histone H2A phosphorylation and H3 methylation are required for a novel Rad9 DSB repair function following checkpoint activation.

Abstract：

:In budding yeast, the Rad9 protein is an important player in the maintenance of genomic integrity and has a well-characterised role in DNA damage checkpoint activation. Recently, roles for different post-translational histone modifications in the DNA damage response, including H2A serine 129 phosphorylation and H3 lysine 79 methylation, have also been demonstrated. Here, we show that Rad9 recruitment to foci and bulk chromatin occurs specifically after ionising radiation treatment in G2 cells. This stable recruitment correlates with late stages of double strand break (DSB) repair and, surprisingly, it is the hypophosphorylated form of Rad9 that is retained on chromatin rather than the hyperphosphorylated, checkpoint-associated, form. Stable Rad9 accumulation in foci requires the Mec1 kinase and two independently regulated histone modifications, H2A phosphorylation and Dot1-dependent H3 methylation. In addition, Rad9 is selectively recruited to a subset of Rad52 repair foci. These results, together with the observation that rad9Delta cells are defective in repair of IR breaks in G2, strongly indicate a novel post checkpoint activation role for Rad9 in promoting efficient repair of DNA DSBs by homologous recombination.

journal_name

DNA Repair (Amst)

journal_title

DNA repair

authors

Toh GW,O'Shaughnessy AM,Jimeno S,Dobbie IM,Grenon M,Maffini S,O'Rorke A,Lowndes NF

doi

10.1016/j.dnarep.2006.03.005

subject

Has Abstract

pub_date

2006-06-10 00:00:00

pages

693-703

issue

6

eissn

1568-7864

issn

1568-7856

pii

S1568-7864(06)00063-2

journal_volume

5

pub_type

杂志文章

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