Minding the gap: the underground functions of BRCA1 and BRCA2 at stalled replication forks.

abstract：:The accuracy of DNA synthesis depends on the accuracy of the polymerase as well as the quality and concentration(s) of the available 5'-deoxynucleoside-triphosphate DNA precursors (dNTPs). The relationships between dNTPs and error rates have been studied in vitro, but only limited insights exist into these correlation...

journal_title：DNA repair

authors： Schaaper RM,Mathews CK

更新日期：2013-01-01 00:00:00

abstract：:AA8 Chinese hamster ovary cells were treated with halogenated nucleosides analogues of thymidine, namely CldU, 5-iodo-2'-deoxyuridine (IdU), and 5-bromo-2'-deoxyuridine (BrdU), following different experimental protocols. The purpose was to see whether incorporation of exogenous pyrimidine analogues into DNA could inte...

journal_title：DNA repair

authors： Cortés F,Pastor N,Mateos S,Domínguez I

更新日期：2003-06-11 00:00:00

abstract：:Ataxia Telangiectasia (A-T) is a progressive childhood disorder characterized most notably by cerebellar degeneration and predisposition to cancer. A-T is caused by mutations in the kinase ATM, a master regulator of the DNA double-strand break response. In addition to DNA-damage signaling defects, A-T cells display mi...

journal_title：DNA repair

authors： Sharma NK,Lebedeva M,Thomas T,Kovalenko OA,Stumpf JD,Shadel GS,Santos JH

更新日期：2014-01-01 00:00:00

abstract：:Triplet repeats undergo frequent mutations in human families afflicted with certain neurodegenerative diseases and also in model organisms. Although the molecular mechanisms of triplet repeat instability are still being identified, it is likely that aberrant DNA synthesis plays an important role. Many DNA polymerases ...

journal_title：DNA repair

authors： Dixon MJ,Lahue RS

更新日期：2002-09-04 00:00:00

abstract：:The DNA polymerase beta (Pol beta) null background renders mouse embryonic fibroblast (MEF) cells base excision repair deficient and hyper-mutagenic upon treatment with the monofunctional alkylating agent, methyl methanesulfonate (MMS). This effect involves an increase in all types of base substitutions, with a modest...

journal_title：DNA repair

authors： Poltoratsky V,Horton JK,Prasad R,Wilson SH

更新日期：2005-09-28 00:00:00

abstract：:Proteins that act on DNA, or are in close proximity to it, can become inadvertently crosslinked to DNA and form highly toxic lesions, known as DNA-protein crosslinks (DPCs). DPCs are generated by different chemotherapeutics, environmental or endogenous sources of crosslinking agents, or by lesions on DNA that stall th...

journal_title：DNA repair

authors： Kühbacher U,Duxin JP

更新日期：2020-10-01 00:00:00

abstract：:DNA damage created by endogenous or exogenous genotoxic agents can exist in multiple forms, and if allowed to persist, can promote genome instability and directly lead to various human diseases, particularly cancer, neurological abnormalities, immunodeficiency and premature aging. To avoid such deleterious outcomes, c...

journal_title：DNA repair

authors： Iyama T,Wilson DM 3rd

更新日期：2013-08-01 00:00:00

abstract：:The proximity of the mitochondrial genome to the respiratory chain, a major source of ROS (radical oxygen species), makes mtDNA more vulnerable to oxidative damage than nuclear DNA. Mitochondrial BER (base excision repair) is generally considered to be the main pathway involved in the prevention of oxidative lesion-in...

journal_title：DNA repair

authors： Kaniak A,Dzierzbicki P,Rogowska AT,Malc E,Fikus M,Ciesla Z

更新日期：2009-03-01 00:00:00

abstract：:Multiple DNA polymerases participate in somatic hypermutation of immunoglobulin (Ig) genes. Mutations at A/T are largely dependent on DNA polymerase eta (POLH) whereas mutations at C/G appear to be generated by several DNA polymerases. We have previously shown that mice expressing a catalytically inactive POLQ (Polq-i...

journal_title：DNA repair

authors： Masuda K,Ouchida R,Hikida M,Nakayama M,Ohara O,Kurosaki T,O-Wang J

更新日期：2006-11-08 00:00:00

abstract：:In a multicellular organism, somatic mutations represent a permanent record of the past chemical and biochemical perturbations experienced by a cell in its local microenvironment. Akin to a perpetual recording device, with every replication, genomic DNA accumulates mutations in patterns that reflect: i) the sequence c...

journal_title：DNA repair

authors： Fedeles BI,Essigmann JM

更新日期：2018-11-01 00:00:00

abstract：:Although correlative studies demonstrate a reduction in the expression of apurinic/apyrimidinic endonuclease/redox effector factor (Ape1/Ref-1 or Ape1) in neural tissues after neuronal insult, the role of Ape1 in regulating neurotoxicity remains to be elucidated. To address this issue, we examined the effects of reduc...

journal_title：DNA repair

authors： Vasko MR,Guo C,Kelley MR

更新日期：2005-03-02 00:00:00

abstract：:The majority of the cells in the body, including stem cells, exist in a quiescent state, so it is in quiescent cells where most DNA damage occurs. It has been uncertain whether or not this damage is repaired or fixed into mutations during quiescence or if proliferation is required for both. Prior to the development of...

journal_title：DNA repair

authors： Bielas JH,Heddle JA

更新日期：2004-07-02 00:00:00

abstract：:Why does a constant barrage of DNA damage lead to disease in some individuals, while others remain healthy? This article surveys current work addressing the implications of inter-individual variation in DNA repair capacity for human health, and discusses the status of DNA repair assays as potential clinical tools for ...

journal_title：DNA repair

authors： Nagel ZD,Chaim IA,Samson LD

更新日期：2014-07-01 00:00:00

abstract：:DNA replication is a prerequisite for cell proliferation, yet it can be increasingly challenging for a eukaryotic cell to faithfully duplicate its genome as its size and complexity expands. Dormant origins now emerge as a key component for cells to successfully accomplish such a demanding but essential task. In this p...

journal_title：DNA repair

authors： Shima N,Pederson KD

更新日期：2017-08-01 00:00:00

abstract：:The mechanisms that allow to circumvent replicative stress, and to resume DNA synthesis are poorly understood in Bacillus subtilis. To study the role of the diadenylate cyclase DisA and branch migration translocase (BMT) RadA/Sms in restarting a stalled replication fork, we nicked and broke the circular chromosome of ...

journal_title：DNA repair

authors： Raguse M,Torres R,Seco EM,Gándara C,Ayora S,Moeller R,Alonso JC

更新日期：2017-11-01 00:00:00

abstract：:The extremely radiation resistant bacterium, Deinococcus radiodurans, contains a spectrum of genes that encode for multiple activities that repair DNA damage. We have cloned and expressed the product of three predicted uracil-DNA glycosylases to determine their biochemical function. DR0689 is a homologue of the Escher...

journal_title：DNA repair

authors： Sandigursky M,Sandigursky S,Sonati P,Daly MJ,Franklin WA

更新日期：2004-02-03 00:00:00

abstract：:Stable expression of Rad51 siRNA was used to generate mouse hybridoma cell lines in which endogenous Rad51 levels were depleted by as much as 60%. Stable Rad51 knockdowns feature reduced homologous recombination responses. The relative ease with which stable Rad51 knockdowns were recovered was surprising, given the em...

journal_title：DNA repair

authors： Magwood AC,Malysewich MJ,Cealic I,Mundia MM,Knapp J,Baker MD

更新日期：2013-12-01 00:00:00

abstract：:8-Oxoguanine (8-oxoG) is a major oxidative lesion produced in DNA by normal cellular metabolism or after exposure to exogenous sources such as ionizing radiation. Persistence of this lesion in DNA causes G to T transversions, with deleterious consequences for the cell. As a result, several repair processes have evolve...

journal_title：DNA repair

authors： Tornaletti S,Maeda LS,Kolodner RD,Hanawalt PC

更新日期：2004-05-04 00:00:00

abstract：:Reactive oxygen species generate ~20,000 oxidative lesions in the DNA of every cell, every day. Most of these lesions are located within nucleosomes, which package DNA in chromatin and impede base excision repair (BER). We demonstrated previously that periodic, spontaneous partial unwrapping of DNA from the underlying...

journal_title：DNA repair

authors： Maher RL,Prasad A,Rizvanova O,Wallace SS,Pederson DS

更新日期：2013-11-01 00:00:00

abstract：:The DNA damage response (DDR) pathway's primary purpose is to maintain the genome structure's integrity and stability. A great deal of effort has done to understand the exact molecular mechanisms of non-coding RNAs, such as lncRNA, miRNAs, and circRNAs, in distinct cellular and genomic processes and cancer progression...

journal_title：DNA repair

authors： Malakoti F,Alemi F,Younesi S,Majidinia M,Yousefi B,Morovat P,Khelghati N,Maleki M,Karimian A,Asemi Z

更新日期：2021-01-07 00:00:00

abstract：:Flaws in the DNA replication process have emerged as a leading driver of genome instability in human diseases. Alteration to replication fork progression is a defining feature of replication stress and the consequent failure to maintain fork integrity and complete genome duplication within a single round of S-phase co...

journal_title：DNA repair

authors： Ait Saada A,Lambert SAE,Carr AM

更新日期：2018-11-01 00:00:00

abstract：:Single-strand-dependent DNA exonucleases play important roles in DNA repair and recombination in all organisms. In Escherichia coli the redundant functions provided by the RecJ, ExoI, ExoVII and ExoX exonucleases are required for mismatch repair, UV resistance and homologous recombination. We have examined whether the...

journal_title：DNA repair

authors： Lombardo MJ,Aponyi I,Ray MP,Sandigursky M,Franklin WA,Rosenberg SM

更新日期：2003-11-21 00:00:00

abstract：:Eukaryotic cells have to regulate the progression and integrity of DNA replication forks through concomitantly transcribed genes. A transcription-dependent increase of recombination within protein-coding and ribosomal genes of eukaryotic cells is well documented. Here we addressed whether tRNA transcription and tRNA-d...

journal_title：DNA repair

authors： de la Loza MC,Wellinger RE,Aguilera A

更新日期：2009-05-01 00:00:00

abstract：:Bacterial MutS2 proteins, consisting of functional domains for ATPase, DNA-binding, and nuclease activities, play roles in DNA recombination and repair. Here we observe a mechanism for generating MutS2 expression diversity in the human pathogen Helicobacter pylori, and identify a unique MutS2 domain responsible for sp...

journal_title：DNA repair

authors： Wang G,Maier RJ

更新日期：2017-09-01 00:00:00

abstract：:Mitochondria are membrane-bound organelles found in eukaryotic cells where they generate energy through the respiratory chain. They contain their own genome that encodes genes critical to the mitochondrial function, but most of their protein content is synthetized from nuclear encoded genes. Damages to the mtDNA can c...

journal_title：DNA repair

authors： Bernal M,Yang X,Lisby M,Mazón G

更新日期：2019-10-01 00:00:00

abstract：:Before a deleterious DNA lesion can be replaced with its undamaged counterpart, the lesion must first be removed from the genome. This process of removing and replacing DNA lesions is accomplished by the careful coordination of several protein factors during DNA repair. One such factor is the multifunctional enzyme hu...

journal_title：DNA repair

authors： Whitaker AM,Freudenthal BD

更新日期：2018-11-01 00:00:00

abstract：:Ubiquitin conjugation plays critical roles in virtually all DNA repair pathways. This review provides an overview of the known multi-domain RING/Ubox E3 ligases and their domain structures. An analysis of known RING/Ubox X-ray and NMR structures leads to a discussion of the effects of dimerization. Structural and mech...

journal_title：DNA repair

authors： Hibbert RG,Mattiroli F,Sixma TK

更新日期：2009-04-05 00:00:00

abstract：:The mechanism of hydrolysis of the apurinic/apyrimidinic (AP) site and its synthetic analogs by using tyrosyl-DNA phosphodiesterase 1 (Tdp1) was analyzed. Tdp1 catalyzes the cleavage of AP site and the synthetic analog of the AP site, 3-hydroxy-2(hydroxymethyl)-tetrahydrofuran (THF), in DNA by hydrolysis of the phosph...

journal_title：DNA repair

authors： Lebedeva NA,Rechkunova NI,Ishchenko AA,Saparbaev M,Lavrik OI

更新日期：2013-12-01 00:00:00

abstract：:OGG1 (8-oxoguanine-DNA glycosylase) is the major DNA repair glycosylase removing the premutagenic DNA base modification 8-oxo-7,8-dihydroguanine (8-oxoG) from the genome of mammalian cells. In addition, there is accumulating evidence that OGG1 and its substrate 8-oxoG might function in the regulation of certain genes,...

journal_title：DNA repair

authors： Seifermann M,Ulges A,Bopp T,Melcea S,Schäfer A,Oka S,Nakabeppu Y,Klungland A,Niehrs C,Epe B

更新日期：2017-10-01 00:00:00

abstract：:Nucleotide excision repair (NER), cell cycle regulation and apoptosis are major defence mechanisms against the carcinogenic effects of UVB radiation. NER eliminates UVB-induced DNA photolesions via two subpathways: global genome repair (GGR) and transcription-coupled repair (TCR). In a previous study, we found UVB-ind...

journal_title：DNA repair

authors： van Oosten M,Stout GJ,Backendorf C,Rebel H,de Wind N,Darroudi F,van Kranen HJ,de Gruijl FR,Mullenders LH

更新日期：2005-01-02 00:00:00