Abstract：
:The RecG protein of Escherichia coli is a double-stranded DNA translocase that unwinds a variety of branched DNAs in vitro, including Holliday junctions, replication forks, D-loops and R-loops. Coupled with the reported pleiotropy of recG mutations, this broad range of potential targets has made it hard to pin down what the protein does in vivo, though roles in recombination and replication fork repair have been suggested. However, recent studies suggest that RecG provides a more general defence against pathological DNA replication. We have postulated that this is achieved through the ability of RecG to eliminate substrates that the replication restart protein, PriA, could otherwise exploit to re-replicate the chromosome. Without RecG, PriA triggers a cascade of events that interfere with the duplication and segregation of chromosomes. Here we review the studies that led us to this idea and to conclude that RecG may be both a specialist activity and a general guardian of the genome.
journal_name
DNA Repair (Amst)journal_title
DNA repairauthors
Rudolph CJ,Upton AL,Briggs GS,Lloyd RGdoi
10.1016/j.dnarep.2009.12.014subject
Has Abstractpub_date
2010-03-02 00:00:00pages
210-23issue
3eissn
1568-7864issn
1568-7856pii
S1568-7864(09)00342-5journal_volume
9pub_type
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