Abstract:
:Oxidative DNA damage is repaired primarily by the base excision repair (BER) pathway in a process initiated by removal of base lesions or mismatched bases by DNA glycosylases. MutY homolog (MYH, MUTYH, or Myh1) is a DNA glycosylase which excises adenine paired with the oxidative lesion 8-oxo-7,8-dihydroguanine (8-oxoG, or G°), thus reducing G:C to T:A mutations. The resulting apurinic/apyrimidinic (AP) site is processed by an AP-endonuclease or a bifunctional glycosylase/lyase. We show here that the major Schizosaccharomyces pombe AP endonuclease, Apn2, binds to the inter-domain connector located between the N- and C-terminal domains of Myh1. This Myh1 inter-domain connector also interacts with the Hus1 subunit of the Rad9-Rad1-Hus1 checkpoint clamp. Mutagenesis studies indicate that Apn2 and Hus1 bind overlapping but different sequence motifs on Myh1. Mutation on I(261) of Myh1 reduces its interaction with Hus1, but only slightly attenuates its interaction with Apn2. However, E(262) of Myh1 is a key determinant for both Apn2 and Hus1 interactions. Like human APE1, Apn2 has 3'-phosphodiesterase activity. However, unlike hAPE1, Apn2 has a weak AP endonuclease activity which cleaves the AP sites generated by Myh1 glycosylase. Functionally, Apn2 stimulates Myh1 glycosylase activity and Apn2 phosphodiesterase activity is stimulated by Myh1. The cross stimulation of Myh1 and Apn2 enzymatic activities is dependent on their physical interaction. Thus, Myh1 and Apn2 constitute an initial BER complex.
journal_name
DNA Repair (Amst)journal_title
DNA repairauthors
Jin J,Hwang BJ,Chang PW,Toth EA,Lu ALdoi
10.1016/j.dnarep.2014.01.001subject
Has Abstractpub_date
2014-03-01 00:00:00pages
1-10eissn
1568-7864issn
1568-7856pii
S1568-7864(14)00002-0journal_volume
15pub_type
杂志文章相关文献
DNA REPAIR文献大全abstract::Detection of γ-H2AX foci as a measure of DNA double strand break induction and repair provides the basis of a rapid approach to establish individual radiosensitivity. However, the assignment of criteria to define increased radiosensitivity is not straightforward. Experimental end points, analytical methods and prolife...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2013.07.002
更新日期:2013-10-01 00:00:00
abstract::Cells carrying deletions of genes encoding H-class ribonucleases display elevated rates of chromosome instability. The role of these enzymes is to remove RNA-DNA associations including persistent mRNA-DNA hybrids (R-loops) formed during transcription, and ribonucleotides incorporated into DNA during replication. RNase...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2017.02.012
更新日期:2017-04-01 00:00:00
abstract::Pathways for tolerating and repairing DNA-protein crosslinks (DPCs) are poorly defined. We used transposon mutagenesis and candidate gene approaches to identify DPC-hypersensitive Escherichia coli mutants. DPCs were induced by azacytidine (aza-C) treatment in cells overexpressing cytosine methyltransferase; hypersensi...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2015.01.016
更新日期:2015-04-01 00:00:00
abstract::The First joint meeting of the German DGDR (German Society for Research on DNA Repair) and the French SFTG (French Society of Genotoxicology) on DNA Repair was held in Toulouse, France, from September 15 to 19, 2007. It was organized by Lisa Wiesmüller and Bernard Salles together with the scientific committee consisti...
journal_title:DNA repair
pub_type:
doi:10.1016/j.dnarep.2007.12.007
更新日期:2008-04-02 00:00:00
abstract::RecQ5 belongs to the RecQ DNA helicase family that includes genes causative of Bloom, Werner, and Rothmund-Thomson syndromes. Although no human disease has been genetically linked to a mutation in RecQ5, Drosophila melanogaster RecQ5 is highly expressed in early embryos, suggesting an important role for it in the DNA ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.10.007
更新日期:2009-02-01 00:00:00
abstract::DNA fiber fluorography is widely employed to study the kinetics of DNA replication, but the usefulness of this approach has been limited by the lack of freely-available automated analysis tools. Quantification of DNA fibers usually relies on manual examination of immunofluorescence microscopy images, which is laboriou...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2019.01.003
更新日期:2019-02-01 00:00:00
abstract::Specialized DNA polymerases are required to bypass DNA damage lesions that would otherwise cause replication arrest and cell death. When operating on non-canonical templates, such as undamaged DNA or on non-cognate lesions, these polymerases exhibit considerably reduced fidelity, resulting in the generation of mutatio...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2005.01.005
更新日期:2005-05-02 00:00:00
abstract::Ataxia Telangiectasia (A-T) is a progressive childhood disorder characterized most notably by cerebellar degeneration and predisposition to cancer. A-T is caused by mutations in the kinase ATM, a master regulator of the DNA double-strand break response. In addition to DNA-damage signaling defects, A-T cells display mi...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2013.11.002
更新日期:2014-01-01 00:00:00
abstract::In male germ cells the repair of DNA double strand breaks (DSBs) differs from that described for somatic cell lines. Irradiation induced immunofluorescent foci (IRIF's) signifying a double strand DNA breaks, were followed in spermatogenic cells up to 16 h after the insult. Foci were characterised for Mdc1, 53BP1 and R...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2007.02.011
更新日期:2007-09-01 00:00:00
abstract::Breast cancer is the most common and significant cancers in females regarding the loss of life quality. Similar to other cancers, one of the etiologic factors in breast cancer is DNA damage. A plethora of molecules are responsible for sensing DNA damage and mediating actions which lead to DNA repair, senescence, cell ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2020.103032
更新日期:2020-12-17 00:00:00
abstract::Me-lex is a sequence-specific alkylating agent synthesized to preferentially (>90%) generate N3-methyladenine (3-mA) in the minor groove of double-strand DNA, in A-T rich regions. In this paper we investigated the effect of XRCC1 deficiency in the processing of 3-mA adducts generated by Me-lex, through the molecular a...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2010.03.016
更新日期:2010-07-01 00:00:00
abstract::Processivity clamps that hold DNA polymerases to DNA for processivity were the first proteins known to encircle the DNA duplex. At the time, polymerase processivity was thought to be the only function of ring shaped processivity clamps. But studies from many laboratories have identified numerous proteins that bind and...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2015.01.015
更新日期:2015-05-01 00:00:00
abstract::Proteins responsible for the integrity of the genome are often used targets in drug therapies against various diseases. The inhibitors of these proteins are also important to study the pathways in genome integrity maintenance. A prominent example is Ugi, a well known cross-species inhibitor protein of the enzyme uraci...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2015.03.005
更新日期:2015-06-01 00:00:00
abstract::Huntington disease (HD) is associated with an unstable trinucleotide CAG.CTG repeat expansion. Although the repeat length is inversely correlated with the age-at-onset of symptoms, variability between patients who have inherited the same HD repeat length clearly suggests that other factors influence this aspect of the...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2007.01.002
更新日期:2007-06-01 00:00:00
abstract::Deinococcus radiodurans survives extremely high doses of ionizing and ultraviolet radiation and treatment with various DNA-damaging chemicals. As an effort to identify and characterize proteins that function in DNA repair in this organism, we have studied the protein encoded by locus DR1572. This gene is predicted to ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.12.011
更新日期:2009-05-01 00:00:00
abstract::Mitochondria are membrane-bound organelles found in eukaryotic cells where they generate energy through the respiratory chain. They contain their own genome that encodes genes critical to the mitochondrial function, but most of their protein content is synthetized from nuclear encoded genes. Damages to the mtDNA can c...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2019.102684
更新日期:2019-10-01 00:00:00
abstract::Eukaryotic cells repair ultraviolet light (UV)- and chemical carcinogen-induced DNA strand-distorting damage through the nucleotide excision repair (NER) pathway. Concurrent activation of the DNA damage checkpoints is also required to arrest the cell cycle and allow time for NER action. Recent studies uncovered critic...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2009.01.011
更新日期:2009-04-05 00:00:00
abstract::O(6)-Alkylguanine-DNA alkyltransferase (MGMT) repairs DNA adducts that result from alkylation at the O(6) position of guanine. These lesions are mutagenic and toxic and can be produced by a variety of agents including the tobacco-specific nitrosamines, carcinogens present in cigarette smoke. Here, we review some of ou...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2007.03.022
更新日期:2007-08-01 00:00:00
abstract::The majority of the cells in the body, including stem cells, exist in a quiescent state, so it is in quiescent cells where most DNA damage occurs. It has been uncertain whether or not this damage is repaired or fixed into mutations during quiescence or if proliferation is required for both. Prior to the development of...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2004.02.010
更新日期:2004-07-02 00:00:00
abstract::DNA polymerases beta (pol beta ) and eta (pol eta ) are the only two eukaryotic polymerases known to efficiently bypass cisplatin and oxaliplatin adducts in vitro. Frameshift errors are an important aspect of mutagenesis. We have compared the types of frameshifts that occur during translesion synthesis past cisplatin ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(02)00150-7
更新日期:2002-12-05 00:00:00
abstract::Xeroderma pigmentosum (XP) is a genetic disorder associated with defects in nucleotide excision repair, a pathway that eliminates a wide variety of helix-distorting DNA lesions, including ultraviolet-induced pyrimidine dimers. In addition to skin diseases in sun-exposed areas, approximately 25% of XP patients develop ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2019.04.004
更新日期:2019-08-01 00:00:00
abstract::Prediabetes is the preclinical stage of type 2 diabetes mellitus (T2DM) with intermediate state of hyperglycemia. Hyperglycemia results in a state of oxidative stress, which may contribute to the production of insulin resistance, β-cell dysfunction and long-term complications of diabetes. Novel approaches are required...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2016.09.004
更新日期:2016-12-01 00:00:00
abstract::Double-strand breaks in genomic DNA (DSB) are potentially lethal lesions which separate parts of chromosome arms from their centromeres. Repair of DSB by recombination can generate mutations and further chromosomal rearrangements, making the regulation of recombination and the choice of recombination pathways of the h...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2011.04.002
更新日期:2011-06-10 00:00:00
abstract::Within a decade the family of AlkB dioxygenases has been extensively studied as a one-protein DNA/RNA repair system in Escherichia coli but also as a group of proteins of much wider functions in eukaryotes. Two strains, HK82 and BS87, are the most commonly used E. coli strains for the alkB gene mutations. The aim of t...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2015.12.010
更新日期:2016-03-01 00:00:00
abstract::The DNA damage response is a key factor in the maintenance of genome stability. As such, it is a central axis in sustaining cellular homeostasis in a variety of contexts: development, growth, differentiation, and maintenance of the normal life cycle of the cell. It is now clear that diverse mechanisms encompassing cel...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2008.03.005
更新日期:2008-07-01 00:00:00
abstract::Proteins that act on DNA, or are in close proximity to it, can become inadvertently crosslinked to DNA and form highly toxic lesions, known as DNA-protein crosslinks (DPCs). DPCs are generated by different chemotherapeutics, environmental or endogenous sources of crosslinking agents, or by lesions on DNA that stall th...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2020.102924
更新日期:2020-10-01 00:00:00
abstract::8-Oxoguanine (8-oxoG) is a major oxidative lesion produced in DNA by normal cellular metabolism or after exposure to exogenous sources such as ionizing radiation. Persistence of this lesion in DNA causes G to T transversions, with deleterious consequences for the cell. As a result, several repair processes have evolve...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2004.01.003
更新日期:2004-05-04 00:00:00
abstract::The oxidation of the thymine methyl group can generate 5-formyluracil (FoU). Template FoU residues are known to miscode, generating base substitution mutations. The repair of the FoU lesion is therefore important in minimizing mutations induced by DNA oxidation. We have studied the repair of FoU in synthetic oligonucl...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(02)00198-2
更新日期:2003-02-03 00:00:00
abstract::Trinucleotide repeat expansions are responsible for at least two dozen neurological disorders. Mechanisms leading to these large expansions of repeated DNA are still poorly understood. It was proposed that transient stalling of the replication fork by the repeat tract might trigger slippage of the newly-synthesized st...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2016.03.002
更新日期:2016-06-01 00:00:00
abstract::3-Nitrobenzanthrone (3-NBA) is a highly mutagenic compound and possible human carcinogen found in diesel exhaust. 3-NBA forms bulky DNA adducts following metabolic activation and induces predominantly G:CT:A transversions in a variety of experimental systems. Here we investigated the influence of nucleotide excision r...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2015.11.004
更新日期:2016-03-01 00:00:00