Abstract:
:Double-strand breaks in genomic DNA (DSB) are potentially lethal lesions which separate parts of chromosome arms from their centromeres. Repair of DSB by recombination can generate mutations and further chromosomal rearrangements, making the regulation of recombination and the choice of recombination pathways of the highest importance. Although knowledge of recombination mechanisms has considerably advanced, the complex interrelationships and regulation of pathways are far from being fully understood. We analyse the different pathways of DSB repair acting in G2/M phase nuclei of irradiated plants, through quantitation of the kinetics of appearance and loss of γ-H2AX foci in Arabidopsis mutants. These analyses show the roles for the four major recombination pathways in post-S-phase DSB repair and that non-homologous recombination pathways constitute the major response. The data suggest a hierarchical organisation of DSB repair in these cells: C-NHEJ acts prior to B-NHEJ which can also inhibit MMEJ. Surprisingly the quadruple ku80 xrcc1 xrcc2 xpf mutant can repair DSB, although with severely altered kinetics. This repair leads to massive genetic instability with more than 50% of mitoses showing anaphase bridges following irradiation. This study thus clarifies the relationships between the different pathways of DSB repair in the living plant and points to the existence of novel DSB repair processes.
journal_name
DNA Repair (Amst)journal_title
DNA repairauthors
Charbonnel C,Allain E,Gallego ME,White CIdoi
10.1016/j.dnarep.2011.04.002subject
Has Abstractpub_date
2011-06-10 00:00:00pages
611-9issue
6eissn
1568-7864issn
1568-7856pii
S1568-7864(11)00085-1journal_volume
10pub_type
杂志文章相关文献
DNA REPAIR文献大全abstract::Non-B DNA conformations adopted by certain types of DNA sequences promote genetic instabilities, especially gross rearrangements including translocations. We conclude the following: (a) slipped (hairpin) structures, cruciforms, triplexes, tetraplexes and i-motifs, and left-handed Z-DNA are formed in chromosomes and el...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2006.05.032
更新日期:2006-09-08 00:00:00
abstract::recX is a small open reading frame located downstream of recA that is conserved in many bacteria. In Escherichia coli, the recX gene (also named oraA) is a 501 bp open reading frame that encodes a predicted basic protein. Transcriptional analysis by Northern blots showed that in E. coli the recX gene is SOS-regulated....
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(02)00217-3
更新日期:2003-03-01 00:00:00
abstract::Among different DNA repair processes that cells use to face with DNA damage, nucleotide excision repair (NER) is particularly important for the removal of a high variety of lesions, including those generated by some antitumor drugs. A number of factors participating in NER, such as the TFIIH complex and the endonuclea...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2017.01.005
更新日期:2017-03-01 00:00:00
abstract::In the midst of the post-war turmoil in Japan, I fortunately followed a path to become a scientist. Sometime at an early stage of my career, I encountered the problem of the cellular response to DNA damage and had the chance to discover a DNA repair enzyme. This event greatly influenced the subsequent course of my res...
journal_title:DNA repair
pub_type: 传,历史文章,杂志文章
doi:10.1016/j.dnarep.2006.03.002
更新日期:2006-06-10 00:00:00
abstract::The RAD6/RAD18 heterodimer promotes translesion synthesis via the monoubiquitination of the DNA sliding clamp, PCNA. In S. cerevisiae, a second complex, UBC13/MMS2/RAD5, can extend this single ubiquitin with a non-canonical lysine 63-linked chain. This polyubiquitination step is required for an error-free mode of bypa...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2004.12.002
更新日期:2005-04-04 00:00:00
abstract::The RAD52 gene is essential for homology-dependent repair of double-strand breaks in Saccharomyces cerevisiae. Rad52 forms complexes with Rad51, replication protein A (RPA) or Rad59 and its presence is essential for the formation of Rad51-Rad52-Rad59 and RPA-Rad52-Rad59 complexes. The N-terminal region of Rad52, which...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/s1568-7864(03)00121-6
更新日期:2003-10-07 00:00:00
abstract::Reactive oxygen species generate ~20,000 oxidative lesions in the DNA of every cell, every day. Most of these lesions are located within nucleosomes, which package DNA in chromatin and impede base excision repair (BER). We demonstrated previously that periodic, spontaneous partial unwrapping of DNA from the underlying...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2013.08.010
更新日期:2013-11-01 00:00:00
abstract::Nucleotide excision repair (NER) is a conserved DNA repair mechanism capable of removing a variety of helix-distorting DNA lesions. Rad26, a member of the Swi2/Snf2 superfamily of proteins, has been shown to be involved in a specialized NER process called transcription coupled NER. Rad16, another member of the same pr...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2007.05.005
更新日期:2007-11-01 00:00:00
abstract::During DNA synthesis in vitro using dNTP and rNTP concentrations present in vivo, yeast replicative DNA polymerases α, δ and ɛ (Pols α, δ and ɛ) stably incorporate rNTPs into DNA. rNTPs are also incorporated during replication in vivo, and they are repaired in an RNase H2-dependent manner. In strains encoding a mutato...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2011.02.001
更新日期:2011-05-05 00:00:00
abstract::Plant mitochondrial and chloroplast genomes encode essential proteins for oxidative phosphorylation and photosynthesis. For proper cellular function, plant organelles must ensure genome integrity. Although plant organelles repair damaged DNA using the multi-enzyme Base Excision Repair (BER) pathway, the details of thi...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2018.02.010
更新日期:2018-05-01 00:00:00
abstract::An important feature of poly(ADP-ribose) polymerases (PARPs) is their ability to readily undergo automodification upon activation. Although a growing number of substrates were found to be poly(ADP-ribosyl)ated, including histones and several DNA damage response factors, PARPs themselves are still considered as the mai...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2015.02.004
更新日期:2015-06-01 00:00:00
abstract::Double-strand breaks (DSBs) are among the most lethal DNA lesions, and a variety of pathways have evolved to manage their repair in a timely fashion. One such pathway is homologous recombination (HR), in which information from an undamaged donor site is used as a template for repair. Although many of the biochemical s...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2017.06.012
更新日期:2017-08-01 00:00:00
abstract::Telomeres play an important role in protecting the ends of chromosomes and preventing chromosome fusion. We have previously demonstrated that double-strand breaks near telomeres in mammalian cells result in either the addition of a new telomere at the site of the break, termed chromosome healing, or sister chromatid f...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.04.004
更新日期:2008-08-02 00:00:00
abstract::In all organisms studied to date, 8-oxoguanine (GO), an important oxidation product of guanine, is removed by highly conserved GO DNA glycosylases. The hyperthermophilic crenarchaeon Pyrobaculum aerophilum encodes a GO DNA glycosylase, Pa-AGOG (Archaeal GO DNA glycosylase) which has become the founding member of a new...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2009.03.009
更新日期:2009-07-04 00:00:00
abstract::Eukaryotic genomes are duplicated by a complex machinery, utilizing high fidelity replicative B-family DNA polymerases (pols) α, δ and ε. Specialized error-prone pol ζ, the fourth B-family member, is recruited when DNA synthesis by the accurate trio is impeded by replication stress or DNA damage. The damage tolerance ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2016.11.004
更新日期:2017-01-01 00:00:00
abstract::DNA mismatch repair is an evolutionarily conserved repair pathway that corrects replication errors. In most prokaryotes and all eukaryotes, the mismatch repair protein MutL is a sequence-unspecific endonuclease that nicks the newly synthesized strand and marks it for repair. Although the sequence of the endonuclease d...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2018.10.003
更新日期:2019-01-01 00:00:00
abstract::XPC is one of the key DNA damage recognition proteins in the global genome repair route of the nucleotide excision repair (NER) pathway. Previously, we demonstrated that NER-deficient mouse models Xpa(-/-) and Xpc(-/-) exhibit a divergent spontaneous tumor spectrum and proposed that XPC might be functionally involved ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2013.08.019
更新日期:2013-12-01 00:00:00
abstract::The extremely radiation resistant bacterium, Deinococcus radiodurans, contains a spectrum of genes that encode for multiple activities that repair DNA damage. We have cloned and expressed the product of three predicted uracil-DNA glycosylases to determine their biochemical function. DR0689 is a homologue of the Escher...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2003.10.011
更新日期:2004-02-03 00:00:00
abstract::Prediabetes is the preclinical stage of type 2 diabetes mellitus (T2DM) with intermediate state of hyperglycemia. Hyperglycemia results in a state of oxidative stress, which may contribute to the production of insulin resistance, β-cell dysfunction and long-term complications of diabetes. Novel approaches are required...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2016.09.004
更新日期:2016-12-01 00:00:00
abstract::DNA damage created by endogenous or exogenous genotoxic agents can exist in multiple forms, and if allowed to persist, can promote genome instability and directly lead to various human diseases, particularly cancer, neurological abnormalities, immunodeficiency and premature aging. To avoid such deleterious outcomes, c...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2013.04.015
更新日期:2013-08-01 00:00:00
abstract::Breast cancer is the most common and significant cancers in females regarding the loss of life quality. Similar to other cancers, one of the etiologic factors in breast cancer is DNA damage. A plethora of molecules are responsible for sensing DNA damage and mediating actions which lead to DNA repair, senescence, cell ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2020.103032
更新日期:2020-12-17 00:00:00
abstract::We have reported that xeroderma pigmentosum group A (Xpa) gene-knockout mice [Xpa (-/-) mice] are deficient in nucleotide excision repair (NER) and highly sensitive to UV-induced skin carcinogenesis. Although xeroderma pigmentosum group A patients show growth retardation, immature sexual development, and neurological ...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.08.003
更新日期:2008-12-01 00:00:00
abstract::Oxidative DNA damage is implicated in brain aging, neurodegeneration and neurological diseases. Damage can be created by normal cellular metabolism, which accumulates with age, or by acute cellular stress conditions which create bursts of oxidative damage. Brain cells have a particularly high basal level of metabolic ...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2013.04.010
更新日期:2013-08-01 00:00:00
abstract::Processivity clamps that hold DNA polymerases to DNA for processivity were the first proteins known to encircle the DNA duplex. At the time, polymerase processivity was thought to be the only function of ring shaped processivity clamps. But studies from many laboratories have identified numerous proteins that bind and...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2015.01.015
更新日期:2015-05-01 00:00:00
abstract::OGG1 (8-oxoguanine-DNA glycosylase) is the major DNA repair glycosylase removing the premutagenic DNA base modification 8-oxo-7,8-dihydroguanine (8-oxoG) from the genome of mammalian cells. In addition, there is accumulating evidence that OGG1 and its substrate 8-oxoG might function in the regulation of certain genes,...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2017.08.005
更新日期:2017-10-01 00:00:00
abstract::Genomic instability has been proposed to play an important role in cancer by accelerating the accumulation of genetic changes responsible for cancer cell evolution. One mechanism for chromosome instability is through the loss of telomeres, which are DNA-protein complexes that protect the ends of chromosomes and preven...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2006.05.030
更新日期:2006-09-08 00:00:00
abstract::Nucleotide excision repair (NER) is a DNA repair pathway, which eliminates various types of helix-distorting DNA damage including some forms of oxidative damage and UV-induced photoproducts. To understand why patients with NER-defective disorders develop progressive neurological abnormalities, we investigated NER capa...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2006.12.006
更新日期:2007-05-01 00:00:00
abstract::All organisms rely on integrated networks to repair DNA double-strand breaks (DSBs) in order to preserve the integrity of the genetic information, to re-establish replication, and to ensure proper chromosomal segregation. Genetic, cytological, biochemical and structural approaches have been used to analyze how Bacillu...
journal_title:DNA repair
pub_type: 杂志文章,评审
doi:10.1016/j.dnarep.2012.12.005
更新日期:2013-03-01 00:00:00
abstract::Translesion DNA polymerases (TLS pols) play critical roles in defense mechanisms against genotoxic agents. The defects or mutations of TLS pols are predicted to result in hypersensitivity of cells to environmental mutagens. In this study, human cells expressing DNA polymerase ζ (Pol ζ) variants with low fidelity or we...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2016.06.002
更新日期:2016-09-01 00:00:00
abstract::The Karpas-620 human myeloma cell line (HMCL) expresses high levels of Cyclin D1 (CCND1), but has a der(8)t(8;11) and a der(14)t(8;14), and not a conventional t(11;14). Fluorescent in situ hybridization (FISH) and array comparative genomic hybridization (aCGH) studies suggest that der(14)t(11;14) from a primary transl...
journal_title:DNA repair
pub_type: 杂志文章
doi:10.1016/j.dnarep.2008.11.010
更新日期:2009-03-01 00:00:00