当前位置： SCI文献检索 > DNA REPAIR期刊下所有文献 > UBE2V2 (MMS2) is not required for effective immunoglobulin gene conversion or DNA damage tolerance in DT40.

UBE2V2 (MMS2) is not required for effective immunoglobulin gene conversion or DNA damage tolerance in DT40.

Abstract：

:The RAD6/RAD18 heterodimer promotes translesion synthesis via the monoubiquitination of the DNA sliding clamp, PCNA. In S. cerevisiae, a second complex, UBC13/MMS2/RAD5, can extend this single ubiquitin with a non-canonical lysine 63-linked chain. This polyubiquitination step is required for an error-free mode of bypass, possibly template switching by the stalled replication complex. Evidence of a role for the human homologue of MMS2, UBE2V2, in such a process has been inferred from the abrogation of ultraviolet light-induced gene conversion following antisense knockdown of the transcript in human fibroblasts. To ask whether a similar mechanism contributes to abasic site-induced immunoglobulin gene conversion, and to ascertain the role of UBE2V2 in the vertebrate DNA damage response we created a ube2v2 mutant of the chicken cell line DT40. Unlike budding yeast mms2, ube2v2 DT40 does not exhibit significant hypersensitivity to DNA damage, nor the elevated sister chromatid exchange seen in vertebrate rad18 mutants suggesting that UBE2V2 plays a minor or redundant role in RAD18 dependent DNA damage tolerance. In addition, both ube2v2 and rad18 DT40 display more or less normal levels of immunoglobulin gene conversion and, despite the important role played by RAD18 in DNA damage induced translesion synthesis, rad18 DT40, unlike rev1 DT40, does not show a defect in non-templated immunoglobulin gene mutation. Together these data suggest that signalling through PCNA ubiquitination is not required for immunoglobulin diversification in DT40.

journal_name

DNA Repair (Amst)

journal_title

DNA repair

authors

Simpson LJ,Sale JE

doi

10.1016/j.dnarep.2004.12.002

keywords:

subject

Has Abstract

pub_date

2005-04-04 00:00:00

pages

503-10

issue

4

eissn

1568-7864

issn

1568-7856

pii

S1568-7864(04)00349-0

journal_volume

4

pub_type

杂志文章

相关文献

DNA REPAIR文献大全
  • xni-deficient Escherichia coli are proficient for recombination and multiple pathways of repair.

    abstract：:Single-strand-dependent DNA exonucleases play important roles in DNA repair and recombination in all organisms. In Escherichia coli the redundant functions provided by the RecJ, ExoI, ExoVII and ExoX exonucleases are required for mismatch repair, UV resistance and homologous recombination. We have examined whether the...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/s1568-7864(03)00135-6

    authors： Lombardo MJ,Aponyi I,Ray MP,Sandigursky M,Franklin WA,Rosenberg SM

    更新日期：2003-11-21 00:00:00

  • Mutational analysis of Thermococcus kodakarensis Endonuclease III reveals the roles of evolutionarily conserved residues.

    abstract：:Endonuclease III (EndoIII) is nearly ubiquitous in all three domains of life. EndoIII family proteins exhibit a bifunctional (glycosylase/lyase) activity on oxidative/saturated pyrimidine bases, such as thymine glycol. Previous studies on EndoIII homologs have reported the presence of important residues involved in su...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2020.102859

    authors： Shiraishi M,Mizutani K,Yamamoto J,Iwai S

    更新日期：2020-06-01 00:00:00

  • Tousled homolog, TLK1, binds and phosphorylates Rad9; TLK1 acts as a molecular chaperone in DNA repair.

    abstract：:The Tousled-like kinases are involved in chromatin assembly, DNA repair, transcription, and chromosome segregation. In this work, we show that overexpression of TLK1B hastens repair of double strand breaks (DSBs) in mouse cells. We have identified Rad9 as a protein interacting tightly with TLK1B. TLK1B phosphorylates ...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2008.09.005

    authors： Sunavala-Dossabhoy G,De Benedetti A

    更新日期：2009-01-01 00:00:00

  • Neurons and astrocytes exhibit lower activities of global genome nucleotide excision repair than do fibroblasts.

    abstract：:Nucleotide excision repair (NER) is a DNA repair pathway, which eliminates various types of helix-distorting DNA damage including some forms of oxidative damage and UV-induced photoproducts. To understand why patients with NER-defective disorders develop progressive neurological abnormalities, we investigated NER capa...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2006.12.006

    authors： Yamamoto A,Nakamura Y,Kobayashi N,Iwamoto T,Yoshioka A,Kuniyasu H,Kishimoto T,Mori T

    更新日期：2007-05-01 00:00:00

  • DNA interstrand crosslinks induce a potent replication block followed by formation and repair of double strand breaks in intact mammalian cells.

    abstract：:DNA interstrand crosslinks (ICLs) are highly toxic lesions that covalently link both strands of DNA and distort the DNA helix. Crosslinking agents have been shown to stall DNA replication and failure to repair ICL lesions before encountered by replication forks may induce severe DNA damage. Most knowledge of the ICL r...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2012.09.010

    authors： Vare D,Groth P,Carlsson R,Johansson F,Erixon K,Jenssen D

    更新日期：2012-12-01 00:00:00

  • Dual DNA-binding domains shape the interaction of Brh2 with DNA.

    abstract：:Brh2, the BRCA2 ortholog in the fungus Ustilago maydis, harbors two different DNA-binding domains, one located in the N-terminal region and the other located in the C-terminal region. Here we were interested in comparing the biochemical properties of Brh2 fragments, Brh2(NT) and Brh2(CT), respectively, harboring the t...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2014.07.013

    authors： Zhou Q,Holloman WK

    更新日期：2014-10-01 00:00:00

  • Proteasome inhibition suppresses DNA-dependent protein kinase activation caused by camptothecin.

    abstract：:The ubiquitin-proteasome pathway plays an important role in DNA damage signaling and repair by facilitating the recruitment and activation of DNA repair factors and signaling proteins at sites of damaged chromatin. Proteasome activity is generally not thought to be required for activation of apical signaling kinases i...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2009.10.008

    authors： Sakasai R,Teraoka H,Tibbetts RS

    更新日期：2010-01-02 00:00:00

  • Caenorhabditis elegans NDX-4 is a MutT-type enzyme that contributes to genomic stability.

    abstract：:MutT enzymes prevent DNA damage by hydrolysis of 8-oxodGTP, an oxidized substrate for DNA synthesis and antimutagenic, anticarcinogenic, and antineurodegenerative functions of MutT enzymes are well established. MutT has been found in almost all kingdoms of life, including many bacterial species, yeasts, plants and mam...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2010.10.009

    authors： Arczewska KD,Baumeier C,Kassahun H,Sengupta T,Bjørås M,Kuśmierek JT,Nilsen H

    更新日期：2011-02-07 00:00:00

  • AHNAK interacts with the DNA ligase IV-XRCC4 complex and stimulates DNA ligase IV-mediated double-stranded ligation.

    abstract：:AHNAK is a high molecular weight protein that is under-expressed in several radiosensitive neuroblastoma cell lines. Using immunoaffinity purification or purified proteins, we show that AHNAK interacts specifically with the DNA ligase IV-XRCC4 complex, a complex that functions in DNA non-homologous end-joining. Furthe...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2003.11.001

    authors： Stiff T,Shtivelman E,Jeggo P,Kysela B

    更新日期：2004-03-04 00:00:00

  • SSB recruitment of Exonuclease I aborts template-switching in Escherichia coli.

    abstract：:Misalignment of a nascent strand and the use of an alternative template during DNA replication, a process termed "template-switching", can give rise to frequent mutations and genetic rearrangements. Mutational hotspots are frequently found associated with imperfect inverted repeats ("quasipalindromes" or "QPs") in man...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2017.05.007

    authors： Laranjo LT,Gross SJ,Zeiger DM,Lovett ST

    更新日期：2017-09-01 00:00:00

  • Interplay of two major repair pathways in the processing of complex double-strand DNA breaks.

    abstract：:Radiation-induced complex double-strand breaks (DSBs) characterised by base lesions, abasic sites or single-strand breaks in close proximity to the break termini, are believed to be a major cause of the biological effects of ionising radiation exposure. It has been hypothesised that complex DSBs pose problems for the ...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2008.05.001

    authors： Dobbs TA,Palmer P,Maniou Z,Lomax ME,O'Neill P

    更新日期：2008-08-02 00:00:00

  • Transcriptional responses to DNA damage.

    abstract：:In response to the threat of DNA damage, cells exhibit a dramatic and multi-factorial response spanning from transcriptional changes to protein modifications, collectively known as the DNA damage response (DDR). Here, we review the literature surrounding the transcriptional response to DNA damage. We review difference...

    journal_title：DNA repair

    pub_type： 杂志文章,评审

    doi：10.1016/j.dnarep.2019.05.002

    authors： Silva E,Ideker T

    更新日期：2019-07-01 00:00:00

  • The role of the DNA damage response in neuronal development, organization and maintenance.

    abstract：:The DNA damage response is a key factor in the maintenance of genome stability. As such, it is a central axis in sustaining cellular homeostasis in a variety of contexts: development, growth, differentiation, and maintenance of the normal life cycle of the cell. It is now clear that diverse mechanisms encompassing cel...

    journal_title：DNA repair

    pub_type： 杂志文章,评审

    doi：10.1016/j.dnarep.2008.03.005

    authors： Barzilai A,Biton S,Shiloh Y

    更新日期：2008-07-01 00:00:00

  • Antibody diversification caused by disrupted mismatch repair and promiscuous DNA polymerases.

    abstract：:The enzyme activation-induced deaminase (AID) targets the immunoglobulin loci in activated B cells and creates DNA mutations in the antigen-binding variable region and DNA breaks in the switch region through processes known, respectively, as somatic hypermutation and class switch recombination. AID deaminates cytosine...

    journal_title：DNA repair

    pub_type： 杂志文章,评审

    doi：10.1016/j.dnarep.2015.11.011

    authors： Zanotti KJ,Gearhart PJ

    更新日期：2016-02-01 00:00:00

  • Endogenous levels of Rad51 and Brca2 are required for homologous recombination and regulated by homeostatic re-balancing.

    abstract：:Stable expression of Rad51 siRNA was used to generate mouse hybridoma cell lines in which endogenous Rad51 levels were depleted by as much as 60%. Stable Rad51 knockdowns feature reduced homologous recombination responses. The relative ease with which stable Rad51 knockdowns were recovered was surprising, given the em...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2013.10.006

    authors： Magwood AC,Malysewich MJ,Cealic I,Mundia MM,Knapp J,Baker MD

    更新日期：2013-12-01 00:00:00

  • Reversibility of replicative senescence in Saccharomyces cerevisiae: effect of homologous recombination and cell cycle checkpoints.

    abstract：:Primary human somatic cells grown in culture divide a finite number of times, exhibiting progressive changes in metabolism and morphology before cessation of cycling. This telomere-initiated cellular senescence occurs because cells have halted production of telomerase, a DNA polymerase required for stabilization of ch...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2011.10.003

    authors： Becerra SC,Thambugala HT,Erickson AR,Lee CK,Lewis LK

    更新日期：2012-01-02 00:00:00

  • Multiple uracil-DNA glycosylase activities in Deinococcus radiodurans.

    abstract：:The extremely radiation resistant bacterium, Deinococcus radiodurans, contains a spectrum of genes that encode for multiple activities that repair DNA damage. We have cloned and expressed the product of three predicted uracil-DNA glycosylases to determine their biochemical function. DR0689 is a homologue of the Escher...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2003.10.011

    authors： Sandigursky M,Sandigursky S,Sonati P,Daly MJ,Franklin WA

    更新日期：2004-02-03 00:00:00

  • Extracts of proliferating and non-proliferating human cells display different base excision pathways and repair fidelity.

    abstract：:Base excision repair (BER) of damaged or inappropriate bases in DNA has been reported to take place by single nucleotide insertion or through incorporation of several nucleotides, termed short-patch and long-patch repair, respectively. We found that extracts from proliferating and non-proliferating cells both had capa...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2009.04.002

    authors： Akbari M,Peña-Diaz J,Andersen S,Liabakk NB,Otterlei M,Krokan HE

    更新日期：2009-07-04 00:00:00

  • Mutational studies of Pa-AGOG DNA glycosylase from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum.

    abstract：:In all organisms studied to date, 8-oxoguanine (GO), an important oxidation product of guanine, is removed by highly conserved GO DNA glycosylases. The hyperthermophilic crenarchaeon Pyrobaculum aerophilum encodes a GO DNA glycosylase, Pa-AGOG (Archaeal GO DNA glycosylase) which has become the founding member of a new...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2009.03.009

    authors： Lingaraju GM,Prota AE,Winkler FK

    更新日期：2009-07-04 00:00:00

  • TP53 and lacZ mutagenesis induced by 3-nitrobenzanthrone in Xpa-deficient human TP53 knock-in mouse embryo fibroblasts.

    abstract：:3-Nitrobenzanthrone (3-NBA) is a highly mutagenic compound and possible human carcinogen found in diesel exhaust. 3-NBA forms bulky DNA adducts following metabolic activation and induces predominantly G:CT:A transversions in a variety of experimental systems. Here we investigated the influence of nucleotide excision r...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2015.11.004

    authors： Kucab JE,Zwart EP,van Steeg H,Luijten M,Schmeiser HH,Phillips DH,Arlt VM

    更新日期：2016-03-01 00:00:00

  • Characterization in vitro and in vivo of the DNA helicase encoded by Deinococcus radiodurans locus DR1572.

    abstract：:Deinococcus radiodurans survives extremely high doses of ionizing and ultraviolet radiation and treatment with various DNA-damaging chemicals. As an effort to identify and characterize proteins that function in DNA repair in this organism, we have studied the protein encoded by locus DR1572. This gene is predicted to ...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2008.12.011

    authors： Cao Z,Julin DA

    更新日期：2009-05-01 00:00:00

  • DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway both in vitro and in vivo.

    abstract：:Deamination of adenine can occur spontaneously under physiological conditions to generate the highly mutagenic lesion, deoxyinosine (hypoxanthine deoxyribonucleotide, dI). In DNA, dI preferably pairs with cytosine rather than thymine and results in A:T to G:C transition mutations after DNA replication. The deamination...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2018.02.005

    authors： Su KY,Lin LI,Goodman SD,Yen RS,Wu CY,Chang WC,Yang YC,Cheng WC,Fang WH

    更新日期：2018-04-01 00:00:00

  • Endogenous expression of phosphorylated histone H2AX in tumors in relation to DNA double-strand breaks and genomic instability.

    abstract：:Microscopically visible gammaH2AX foci signify the presence of DNA double-strand breaks (dsbs) in irradiated cells. However, large foci are also observed in untreated tumour cells, and high numbers reduce the sensitivity for detecting drug or radiation-induced DNA breaks. SW756 cervical carcinoma cells that express ab...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2006.05.040

    authors： Yu T,MacPhail SH,Banáth JP,Klokov D,Olive PL

    更新日期：2006-08-13 00:00:00

  • RecA mediated initial alignment of homologous DNA molecules displays apparent first order kinetics with little effect of heterology.

    abstract：:The mechanism and determinants of RecA mediated initial alignment of homologous DNA molecules were studied by performing Monte Carlo simulations of the dynamics of DNA molecules. The simulation procedure was used to assess the effect of heterologous DNA and dilution on the rate of formation and yield of homologous ali...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2003.09.009

    authors： Patel S,Edwards JS

    更新日期：2004-01-05 00:00:00

  • The dCMP transferase activity of yeast Rev1 is biologically relevant during the bypass of endogenously generated AP sites.

    abstract：:The bypass of AP sites in yeast requires the Rev1 protein in addition to the Pol ζ translesion synthesis DNA polymerase. Although Rev1 was originally characterized biochemically as a dCMP transferase during AP-site bypass, the relevance of this activity in vivo is unclear. The current study uses highly sensitive frame...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2011.09.017

    authors： Kim N,Mudrak SV,Jinks-Robertson S

    更新日期：2011-12-10 00:00:00

  • Replication stalling and heteroduplex formation within CAG/CTG trinucleotide repeats by mismatch repair.

    abstract：:Trinucleotide repeat expansions are responsible for at least two dozen neurological disorders. Mechanisms leading to these large expansions of repeated DNA are still poorly understood. It was proposed that transient stalling of the replication fork by the repeat tract might trigger slippage of the newly-synthesized st...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2016.03.002

    authors： Viterbo D,Michoud G,Mosbach V,Dujon B,Richard GF

    更新日期：2016-06-01 00:00:00

  • In vitro and in vivo studies of MutS, MutL and MutH mutants: correlation of mismatch repair and DNA recombination.

    abstract：:We have used the recently determined crystal structures of Escherichia coli (E. coli) MutS, MutL and MutH to guide construction of 47 amino-acid substitutions in these proteins and analyzed their behavior in mismatch repair and recombination in vitro and in vivo. We find that the active site of the MutH endonuclease i...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/s1568-7864(02)00245-8

    authors： Junop MS,Yang W,Funchain P,Clendenin W,Miller JH

    更新日期：2003-04-02 00:00:00

  • Regulation of DNA damage response pathways by the cullin-RING ubiquitin ligases.

    abstract：:Eukaryotic cells repair ultraviolet light (UV)- and chemical carcinogen-induced DNA strand-distorting damage through the nucleotide excision repair (NER) pathway. Concurrent activation of the DNA damage checkpoints is also required to arrest the cell cycle and allow time for NER action. Recent studies uncovered critic...

    journal_title：DNA repair

    pub_type： 杂志文章,评审

    doi：10.1016/j.dnarep.2009.01.011

    authors： Hannah J,Zhou P

    更新日期：2009-04-05 00:00:00

  • DNA mismatch repair preferentially safeguards actively transcribed genes.

    abstract：:DNA mismatch repair (MMR) is an evolutionally conserved genome maintenance pathway and is well known for its role in maintaining replication fidelity by correcting biosynthetic errors generated during DNA replication. However, recent studies have shown that MMR preferentially protects actively transcribed genes from m...

    journal_title：DNA repair

    pub_type： 杂志文章,评审

    doi：10.1016/j.dnarep.2018.08.010

    authors： Huang Y,Li GM

    更新日期：2018-11-01 00:00:00

  • Activation of cellular signaling by 8-oxoguanine DNA glycosylase-1-initiated DNA base excision repair.

    abstract：:Accumulation of 8-oxo-7,8-dihydroguanine (8-oxoG) in the DNA results in genetic instability and mutagenesis, and is believed to contribute to carcinogenesis, aging processes and various aging-related diseases. 8-OxoG is removed from the DNA via DNA base excision repair (BER), initiated by 8-oxoguanine DNA glycosylase-...

    journal_title：DNA repair

    pub_type： 杂志文章

    doi：10.1016/j.dnarep.2013.06.006

    authors： German P,Szaniszlo P,Hajas G,Radak Z,Bacsi A,Hazra TK,Hegde ML,Ba X,Boldogh I

    更新日期：2013-10-01 00:00:00