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RecA mediated initial alignment of homologous DNA molecules displays apparent first order kinetics with little effect of heterology.

Abstract：

:The mechanism and determinants of RecA mediated initial alignment of homologous DNA molecules were studied by performing Monte Carlo simulations of the dynamics of DNA molecules. The simulation procedure was used to assess the effect of heterologous DNA and dilution on the rate of formation and yield of homologous alignments. The results show that the apparent first order kinetic behavior and the impact of heterologous DNA, reported in literature [J. Biol. Chem. 261 (1986) 1025], can be observed even if the conversion of the initially aligned molecules into a stable joint is not rate-determining. The present study is the first step towards developing rigorous computational models to describe the process of homologous recombination, and theoretical frameworks to retrieve biophysical parameters of strand pairing and exchange proteins from in vitro assays of joint molecule formation.

journal_name

DNA Repair (Amst)

journal_title

DNA repair

authors

Patel S,Edwards JS

doi

10.1016/j.dnarep.2003.09.009

keywords:

subject

Has Abstract

pub_date

2004-01-05 00:00:00

pages

61-5

issue

1

eissn

1568-7864

issn

1568-7856

pii

S1568786403002039

journal_volume

3

pub_type

杂志文章

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