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An open-source algorithm for rapid unbiased determination of DNA fiber length.

Abstract：

:DNA fiber fluorography is widely employed to study the kinetics of DNA replication, but the usefulness of this approach has been limited by the lack of freely-available automated analysis tools. Quantification of DNA fibers usually relies on manual examination of immunofluorescence microscopy images, which is laborious and prone to inter- and intra-operator variability. To address this, we developed an unbiased, fully automated algorithm that quantifies length and color of DNA fibers from fluorescence microscopy images. Our fiber quantification method, termed FiberQ, is an open-source image processing tool based on edge detection and a novel segment splicing approach. Here, we describe the algorithm in detail, validate our results experimentally, and benchmark the analysis against manual assessments. Our implementation is offered free of charge to the scientific community under the General Public License.

journal_name

DNA Repair (Amst)

journal_title

DNA repair

authors

Ghesquière P,Elsherbiny A,Fortier E,McQuaid M,Mazzaferri J,Bélanger F,Cheriet F,Drobetsky E,Wurtele H,Costantino S

doi

10.1016/j.dnarep.2019.01.003

subject

Has Abstract

pub_date

2019-02-01 00:00:00

pages

26-37

eissn

1568-7864

issn

1568-7856

pii

S1568-7864(18)30236-2

journal_volume

74

pub_type

杂志文章

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