Abstract：
:The continuity of duplex DNA is generally considered a prerequisite for chromosome continuity. However, as previously shown in yeast as well as human cells, the introduction of a double-strand break (DSB) does not generate a chromosome break (CRB) in yeast or human cells. The transition from DSB to CRB was found to be under limited control by the tethering function of the RAD50/MRE11/XRS2 (MRX) complex. Using a system for differential fluorescent marking of both sides of an endonuclease-induced DSB in single cells, we found that nearly all DSBs are converted to CRBs in cells lacking both exonuclease 1 (EXO1) activity and MRX complex. Thus, it appears that some feature of exonuclease processing or resection at a DSB is critical for maintaining broken chromosome ends in close proximity. In addition, we discovered a thermal sensitive (cold) component to CRB formation in an MRX mutant that has implications for chromosome end mobility and/or end-processing.
journal_name
DNA Repair (Amst)journal_title
DNA repairauthors
Nakai W,Westmoreland J,Yeh E,Bloom K,Resnick MAdoi
10.1016/j.dnarep.2010.10.004subject
Has Abstractpub_date
2011-01-02 00:00:00pages
102-10issue
1eissn
1568-7864issn
1568-7856pii
S1568-7864(10)00355-1journal_volume
10pub_type
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