Deficient global genome repair of UV-induced cyclobutane pyrimidine dimers in terminally differentiated myocytes and proliferating fibroblasts from the rat heart.

Abstract:

:Nucleotide excision repair (NER) is the principal pathway for the removal of a wide range of DNA helix-distorting lesions. Two NER subpathways have been identified, i.e. global genome repair (GGR) and transcription-coupled repair (TCR). Little is known about the expression of NER pathways in differentiated cells. We assessed the repair of UV-induced cyclobutane pyrimidine dimers (CPD) and 6-4-photoproducts (6-4 PP) in terminally differentiated myocytes and proliferating fibroblasts isolated from the hearts of neonatal rats. Myocytes and fibroblasts were found to carry out efficient removal of 6-4 PP but display poor repair of CPD by GGR. Furthermore, both cell types were found to carry out TCR of CPD, thus mimicking the repair phenotype of established rodent cell lines. The inefficient repair of CPD at the genome overall level occurs in the absence of massive apoptosis, but goes along with an undetectable level of transcription of the p48 gene, known to be mutated in xeroderma pigmentosum group E (XP-E) patients and recently proposed to be essential for repair of CPD in nonexpressed DNA. Taken together, the results suggest that primary non-dividing cardiac myocytes and proliferating fibroblasts from rat heart selectively remove CPD from the transcribed strand of transcriptionally active genes. GGR of CPD is poor due to the absence of p48 expression.

journal_name

DNA Repair (Amst)

journal_title

DNA repair

authors

van der Wees CG,Vreeswijk MP,Persoon M,van der Laarse A,van Zeeland AA,Mullenders LH

doi

10.1016/j.dnarep.2003.06.001

keywords:

subject

Has Abstract

pub_date

2003-12-09 00:00:00

pages

1297-308

issue

12

eissn

1568-7864

issn

1568-7856

pii

S1568786403001599

journal_volume

2

pub_type

杂志文章
  • Real-time investigation of the roles of ATP hydrolysis by UvrA and UvrB during DNA damage recognition in nucleotide excision repair.

    abstract::Nucleotide excision repair (NER) stands out among other DNA repair systems for its ability to process a diverse set of unrelated DNA lesions. In bacteria, NER damage detection is orchestrated by the UvrA and UvrB proteins, which form the UvrA2-UvrB2 (UvrAB) damage sensing complex. The highly versatile damage recogniti...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2020.103024

    authors: Kraithong T,Sucharitakul J,Buranachai C,Jeruzalmi D,Chaiyen P,Pakotiprapha D

    更新日期:2021-01-01 00:00:00

  • Multiple pathways cooperate to facilitate DNA replication fork progression through alkylated DNA.

    abstract::Eukaryotic genomes are especially vulnerable to DNA damage during the S phase of the cell cycle, when chromosomes must be duplicated. The stability of DNA replication forks is critical to achieve faithful chromosome replication and is severely compromised when forks encounter DNA lesions. To maintain genome integrity,...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2008.06.014

    authors: Vázquez MV,Rojas V,Tercero JA

    更新日期:2008-10-01 00:00:00

  • Regulation of NAD+ metabolism, signaling and compartmentalization in the yeast Saccharomyces cerevisiae.

    abstract::Pyridine nucleotides are essential coenzymes in many cellular redox reactions in all living systems. In addition to functioning as a redox carrier, NAD(+) is also a required co-substrate for the conserved sirtuin deacetylases. Sirtuins regulate transcription, genome maintenance and metabolism and function as molecular...

    journal_title:DNA repair

    pub_type: 杂志文章,评审

    doi:10.1016/j.dnarep.2014.07.009

    authors: Kato M,Lin SJ

    更新日期:2014-11-01 00:00:00

  • Conserved helicase domain of human RecQ4 is required for strand annealing-independent DNA unwinding.

    abstract::Humans have five members of the well conserved RecQ helicase family: RecQ1, Bloom syndrome protein (BLM), Werner syndrome protein (WRN), RecQ4, and RecQ5, which are all known for their roles in maintaining genome stability. BLM, WRN, and RecQ4 are associated with premature aging and cancer predisposition. Of the three...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2010.04.003

    authors: Rossi ML,Ghosh AK,Kulikowicz T,Croteau DL,Bohr VA

    更新日期:2010-07-01 00:00:00

  • Paradoxical roles of cyclin D1 in DNA stability.

    abstract::Maintenance of DNA integrity is vital for all of the living organisms. Consequence of DNA damaging ranges from, introducing harmless synonymous mutations, to causing disease-associated mutations, genome instability, and cell death. A cell cycle protein cyclin D1 is an established cancer-driving protein. However, contr...

    journal_title:DNA repair

    pub_type: 杂志文章,评审

    doi:10.1016/j.dnarep.2016.04.011

    authors: Jirawatnotai S,Sittithumcharee G

    更新日期:2016-06-01 00:00:00

  • DNA damage in blood cells in relation to chemotherapy and nutritional status in colorectal cancer patients-A pilot study.

    abstract::DNA damage can be considered as a biomarker for toxicity and response to chemotherapy. It is not known whether the chemotherapy-induced genotoxicity is associated with malnutrition. In this pilot study, we assess genotoxicity by means of DNA damage in patients with lymph-node positive colorectal cancer (CRC) and explo...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2018.01.005

    authors: Kværner AS,Minaguchi J,Yamani NE,Henriksen C,Ræder H,Paur I,Henriksen HB,Wiedswang G,Smeland S,Blomhoff R,Collins AR,Bøhn SK

    更新日期:2018-03-01 00:00:00

  • Human MutS and FANCM complexes function as redundant DNA damage sensors in the Fanconi Anemia pathway.

    abstract::The Fanconi Anemia (FA) pathway encodes a DNA damage response activated by DNA damage-stalled replication forks. Current evidence suggests that the FA pathway initiates with DNA damage recognition by the FANCM complex (FANCM/FAAP24/MHF). However, genetic inactivation of FANCM in mouse and DT40 cells causes only a part...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2011.09.006

    authors: Huang M,Kennedy R,Ali AM,Moreau LA,Meetei AR,D'Andrea AD,Chen CC

    更新日期:2011-12-10 00:00:00

  • Schizosaccharomyces pombe Mms1 channels repair of perturbed replication into Rhp51 independent homologous recombination.

    abstract::In both Schizosaccharomyces pombe and Saccharomyces cerevisiae, Mms22 and Mms1 form a complex with important functions in the response to DNA damage, loss of which leads to perturbations during replication. Furthermore, in S. cerevisiae, Mms1 has been suggested to function in concert with a Cullin-like protein, Rtt101...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2010.11.013

    authors: Vejrup-Hansen R,Mizuno K,Miyabe I,Fleck O,Holmberg C,Murray JM,Carr AM,Nielsen O

    更新日期:2011-03-07 00:00:00

  • APE1: A skilled nucleic acid surgeon.

    abstract::Before a deleterious DNA lesion can be replaced with its undamaged counterpart, the lesion must first be removed from the genome. This process of removing and replacing DNA lesions is accomplished by the careful coordination of several protein factors during DNA repair. One such factor is the multifunctional enzyme hu...

    journal_title:DNA repair

    pub_type: 杂志文章,评审

    doi:10.1016/j.dnarep.2018.08.012

    authors: Whitaker AM,Freudenthal BD

    更新日期:2018-11-01 00:00:00

  • Yeast genes involved in cadmium tolerance: Identification of DNA replication as a target of cadmium toxicity.

    abstract::Cadmium (Cd(2+)) is a ubiquitous environmental pollutant and human carcinogen. The molecular basis of its toxicity remains unclear. Here, to identify the landscape of genes and cell functions involved in cadmium resistance, we have screened the Saccharomyces cerevisiae deletion collection for mutants sensitive to cadm...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2008.04.005

    authors: Serero A,Lopes J,Nicolas A,Boiteux S

    更新日期:2008-08-02 00:00:00

  • The role of the SWI/SNF chromatin remodelling complex in the response to DNA double strand breaks.

    abstract::Mammalian cells possess multiple closely related SWI/SNF chromatin remodelling complexes. These complexes have been implicated in the cellular response to DNA double strand breaks (DSBs). Evidence suggests that SWI/SNF complexes contribute to successful repair via both the homologous recombination and non-homologous e...

    journal_title:DNA repair

    pub_type: 杂志文章,评审

    doi:10.1016/j.dnarep.2020.102919

    authors: Harrod A,Lane KA,Downs JA

    更新日期:2020-09-01 00:00:00

  • Telomeres and chromosome instability.

    abstract::Genomic instability has been proposed to play an important role in cancer by accelerating the accumulation of genetic changes responsible for cancer cell evolution. One mechanism for chromosome instability is through the loss of telomeres, which are DNA-protein complexes that protect the ends of chromosomes and preven...

    journal_title:DNA repair

    pub_type: 杂志文章,评审

    doi:10.1016/j.dnarep.2006.05.030

    authors: Murnane JP

    更新日期:2006-09-08 00:00:00

  • Characterization in vitro and in vivo of the DNA helicase encoded by Deinococcus radiodurans locus DR1572.

    abstract::Deinococcus radiodurans survives extremely high doses of ionizing and ultraviolet radiation and treatment with various DNA-damaging chemicals. As an effort to identify and characterize proteins that function in DNA repair in this organism, we have studied the protein encoded by locus DR1572. This gene is predicted to ...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2008.12.011

    authors: Cao Z,Julin DA

    更新日期:2009-05-01 00:00:00

  • Intrinsic mitochondrial DNA repair defects in Ataxia Telangiectasia.

    abstract::Ataxia Telangiectasia (A-T) is a progressive childhood disorder characterized most notably by cerebellar degeneration and predisposition to cancer. A-T is caused by mutations in the kinase ATM, a master regulator of the DNA double-strand break response. In addition to DNA-damage signaling defects, A-T cells display mi...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2013.11.002

    authors: Sharma NK,Lebedeva M,Thomas T,Kovalenko OA,Stumpf JD,Shadel GS,Santos JH

    更新日期:2014-01-01 00:00:00

  • DNA mismatch repair preferentially safeguards actively transcribed genes.

    abstract::DNA mismatch repair (MMR) is an evolutionally conserved genome maintenance pathway and is well known for its role in maintaining replication fidelity by correcting biosynthetic errors generated during DNA replication. However, recent studies have shown that MMR preferentially protects actively transcribed genes from m...

    journal_title:DNA repair

    pub_type: 杂志文章,评审

    doi:10.1016/j.dnarep.2018.08.010

    authors: Huang Y,Li GM

    更新日期:2018-11-01 00:00:00

  • Lung cancer risk and variation in MGMT activity and sequence.

    abstract::O(6)-Alkylguanine-DNA alkyltransferase (MGMT) repairs DNA adducts that result from alkylation at the O(6) position of guanine. These lesions are mutagenic and toxic and can be produced by a variety of agents including the tobacco-specific nitrosamines, carcinogens present in cigarette smoke. Here, we review some of ou...

    journal_title:DNA repair

    pub_type: 杂志文章,评审

    doi:10.1016/j.dnarep.2007.03.022

    authors: Povey AC,Margison GP,Santibáñez-Koref MF

    更新日期:2007-08-01 00:00:00

  • Absence of DNA polymerase theta results in decreased somatic hypermutation frequency and altered mutation patterns in Ig genes.

    abstract::Multiple DNA polymerases participate in somatic hypermutation of immunoglobulin (Ig) genes. Mutations at A/T are largely dependent on DNA polymerase eta (POLH) whereas mutations at C/G appear to be generated by several DNA polymerases. We have previously shown that mice expressing a catalytically inactive POLQ (Polq-i...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2006.06.006

    authors: Masuda K,Ouchida R,Hikida M,Nakayama M,Ohara O,Kurosaki T,O-Wang J

    更新日期:2006-11-08 00:00:00

  • Tousled homolog, TLK1, binds and phosphorylates Rad9; TLK1 acts as a molecular chaperone in DNA repair.

    abstract::The Tousled-like kinases are involved in chromatin assembly, DNA repair, transcription, and chromosome segregation. In this work, we show that overexpression of TLK1B hastens repair of double strand breaks (DSBs) in mouse cells. We have identified Rad9 as a protein interacting tightly with TLK1B. TLK1B phosphorylates ...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2008.09.005

    authors: Sunavala-Dossabhoy G,De Benedetti A

    更新日期:2009-01-01 00:00:00

  • Mutational consequences of dNTP pool imbalances in E. coli.

    abstract::The accuracy of DNA synthesis depends on the accuracy of the polymerase as well as the quality and concentration(s) of the available 5'-deoxynucleoside-triphosphate DNA precursors (dNTPs). The relationships between dNTPs and error rates have been studied in vitro, but only limited insights exist into these correlation...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2012.10.011

    authors: Schaaper RM,Mathews CK

    更新日期:2013-01-01 00:00:00

  • Cross-species inhibition of dUTPase via the Staphylococcal Stl protein perturbs dNTP pool and colony formation in Mycobacterium.

    abstract::Proteins responsible for the integrity of the genome are often used targets in drug therapies against various diseases. The inhibitors of these proteins are also important to study the pathways in genome integrity maintenance. A prominent example is Ugi, a well known cross-species inhibitor protein of the enzyme uraci...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2015.03.005

    authors: Hirmondó R,Szabó JE,Nyíri K,Tarjányi S,Dobrotka P,Tóth J,Vértessy BG

    更新日期:2015-06-01 00:00:00

  • xni-deficient Escherichia coli are proficient for recombination and multiple pathways of repair.

    abstract::Single-strand-dependent DNA exonucleases play important roles in DNA repair and recombination in all organisms. In Escherichia coli the redundant functions provided by the RecJ, ExoI, ExoVII and ExoX exonucleases are required for mismatch repair, UV resistance and homologous recombination. We have examined whether the...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/s1568-7864(03)00135-6

    authors: Lombardo MJ,Aponyi I,Ray MP,Sandigursky M,Franklin WA,Rosenberg SM

    更新日期:2003-11-21 00:00:00

  • Guanine repeat-containing sequences confer transcription-dependent instability in an orientation-specific manner in yeast.

    abstract::Non-B DNA structures are a major contributor to the genomic instability associated with repetitive sequences. Immunoglobulin switch Mu (Sμ) region sequence is comprised of guanine-rich repeats and has high potential for forming G4 DNA, in which one strand of DNA folds into an array of guanine quartets. Taking advantag...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2011.07.002

    authors: Kim N,Jinks-Robertson S

    更新日期:2011-09-05 00:00:00

  • Molecular basis for the functions of a bacterial MutS2 in DNA repair and recombination.

    abstract::Bacterial MutS2 proteins, consisting of functional domains for ATPase, DNA-binding, and nuclease activities, play roles in DNA recombination and repair. Here we observe a mechanism for generating MutS2 expression diversity in the human pathogen Helicobacter pylori, and identify a unique MutS2 domain responsible for sp...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2017.07.004

    authors: Wang G,Maier RJ

    更新日期:2017-09-01 00:00:00

  • Biochemical reconstitution and genetic characterization of the major oxidative damage base excision DNA repair pathway in Thermococcus kodakarensis.

    abstract::Reactive oxygen species drive the oxidation of guanine to 8-oxoguanine (8oxoG), which threatens genome integrity. The repair of 8oxoG is carried out by base excision repair enzymes in Bacteria and Eukarya, however, little is known about archaeal 8oxoG repair. This study identifies a member of the Ogg-subfamily archaea...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2019.102767

    authors: Gehring AM,Zatopek KM,Burkhart BW,Potapov V,Santangelo TJ,Gardner AF

    更新日期:2020-02-01 00:00:00

  • XRCC1 protein; Form and function.

    abstract::The human gene that encodes XRCC1 was cloned nearly thirty years ago but experimental analysis of this fascinating protein is still unveiling new insights into the DNA damage response. XRCC1 is a molecular scaffold protein that interacts with multiple enzymatic components of DNA single-strand break repair (SSBR) inclu...

    journal_title:DNA repair

    pub_type: 杂志文章,评审

    doi:10.1016/j.dnarep.2019.102664

    authors: Caldecott KW

    更新日期:2019-09-01 00:00:00

  • Homologous recombination and the yKu70/80 complex exert opposite roles in resistance against the killer toxin from Pichia acaciae.

    abstract::The linear plasmid (pPac1-2) encoded killer toxin (PaT) of the yeast Pichia acaciae arrests sensitive Saccharomyces cerevisiae cells in the S-phase of the cell cycle and induces mutations. Here we provide evidence for opposite effects in PaT resistance of homologous recombination (HR) and non-homologous end joining (N...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2007.07.010

    authors: Klassen R,Krampe S,Meinhardt F

    更新日期:2007-12-01 00:00:00

  • Archaeal DNA uracil repair via direct strand incision: A minimal system reconstituted from purified components.

    abstract::Hydrolytic deamination of DNA cytosine residues results in U/G mispairs, pre-mutagenic lesions threatening long-term genetic stability. Hence, DNA uracil repair is ubiquitous throughout all extant life forms and base excision repair, triggered by a uracil DNA glycosylase (UDG), is the mechanistic paradigm adopted, as ...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2010.01.004

    authors: Schomacher L,Schürer KA,Ciirdaeva E,McDermott P,Chong JP,Kramer W,Fritz HJ

    更新日期:2010-04-04 00:00:00

  • The involvement of key DNA repair pathways in the formation of chromosome rearrangements in embryonic stem cells.

    abstract::It is vital that embryonic stem (ES) cells, which give rise to the diverse tissues of the mature organism, maintain genetic stability. To understand mechanisms for the prevention and causation of chromosomal instability, we have used spectral karyotyping (SKY) to analyse ES cells from wild-type and repair-gene knockou...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2005.05.005

    authors: Griffin C,Waard Hd,Deans B,Thacker J

    更新日期:2005-08-15 00:00:00

  • Srs2 overexpression reveals a helicase-independent role at replication forks that requires diverse cell functions.

    abstract::Srs2 is a 3'-5' DNA helicase that regulates many aspects of DNA metabolism in Saccharomyces cerevisiae. It is best known for its ability to counteract homologous recombination by dismantling Rad51 filaments, but is also involved in checkpoint activation, adaptation and recovery, and in resolution of late recombination...

    journal_title:DNA repair

    pub_type: 杂志文章

    doi:10.1016/j.dnarep.2011.02.004

    authors: León Ortiz AM,Reid RJ,Dittmar JC,Rothstein R,Nicolas A

    更新日期:2011-05-05 00:00:00

  • Structural aspects of multi-domain RING/Ubox E3 ligases in DNA repair.

    abstract::Ubiquitin conjugation plays critical roles in virtually all DNA repair pathways. This review provides an overview of the known multi-domain RING/Ubox E3 ligases and their domain structures. An analysis of known RING/Ubox X-ray and NMR structures leads to a discussion of the effects of dimerization. Structural and mech...

    journal_title:DNA repair

    pub_type: 杂志文章,评审

    doi:10.1016/j.dnarep.2009.01.014

    authors: Hibbert RG,Mattiroli F,Sixma TK

    更新日期:2009-04-05 00:00:00