Abstract:
:Molecular characterization of the coral host and the microbial assemblages associated with it (referred to as the coral holobiont) is currently undertaken via marker gene sequencing. This requires bulky instruments and controlled laboratory conditions which are impractical for environmental experiments in remote areas. Recent advances in sequencing technologies now permit rapid sequencing in the field; however, development of specific protocols and pipelines for the effective processing of complex microbial systems are currently lacking. Here, we used a combination of 3 marker genes targeting the coral animal host, its symbiotic alga, and the associated bacterial microbiome to characterize 60 coral colonies collected and processed in situ, during the Tara Pacific expedition. We used Oxford Nanopore Technologies to sequence marker gene amplicons and developed bioinformatics pipelines to analyze nanopore reads on a laptop, obtaining results in less than 24 h. Reef scale network analysis of coral-associated bacteria reveals broadly distributed taxa, as well as host-specific associations. Protocols and tools used in this work may be applicable for rapid coral holobiont surveys, immediate adaptation of sampling strategy in the field, and to make informed and timely decisions in the context of the current challenges affecting coral reefs worldwide.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Carradec Q,Poulain J,Boissin E,Hume BCC,Voolstra CR,Ziegler M,Engelen S,Cruaud C,Planes S,Wincker Pdoi
10.1038/s41598-020-72589-0subject
Has Abstractpub_date
2020-09-28 00:00:00pages
15893issue
1issn
2045-2322pii
10.1038/s41598-020-72589-0journal_volume
10pub_type
杂志文章abstract::Plants adapted to extreme conditions can be at high risk from climate change; arctic-alpine plants, in particular, could "run out of space" as they are out-competed by expansion of woody vegetation. Mountain regions could potentially provide safe sites for arctic-alpine plants in a warmer climate, but empirical eviden...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55989-9
更新日期:2019-12-23 00:00:00
abstract::FGF10 is a member of fibroblast growth factors (FGFs). We previously showed that FGF10 protects neuron against oxygen-glucose deprivation injury in vitro; however, the effect of FGF10 in ischemic stroke in vivo is unknown. In the present study, we showed that FGF10 was mainly expressed in neurons but not astrocytes, a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19869
更新日期:2016-01-27 00:00:00
abstract::Clogging of porous media by soft particles has become a subject of extensive research in the last years and the understanding of the clogging mechanisms is of great importance for process optimization. The rise in the utilization of microfluidic devices brought the possibility to simulate membrane filtration and perfo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45791-y
更新日期:2019-06-25 00:00:00
abstract::Variability in the x-ray tube current used in computed tomography may affect quantitative features extracted from the images. To investigate these effects, we scanned the Credence Cartridge Radiomics phantom 12 times, varying the tube current from 25 to 300 mA∙s while keeping the other acquisition parameters constant....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20713-6
更新日期:2018-02-05 00:00:00
abstract::For the first time, soil core samples from the Jiuquan region have been analyzed for Pu isotopes for radioactive source identification and radiological assessment. The Jiuquan region is in downwind from the Lop Nor Chinese nuclear test (CNT) site. The high Pu inventories (13 to 546 Bq/m(2)) in most of the sampling loc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12262
更新日期:2015-07-17 00:00:00
abstract::Functional magnetic resonance imaging (fMRI) based on the blood oxygen level dependent (BOLD) contrast has gained a prominent position in neuroscience for imaging neuronal activity and studying effective brain connectivity under working state and functional connectivity at resting state. However, the fundamental quest...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02864-0
更新日期:2017-06-02 00:00:00
abstract::We have studied both experimentally and theoretically the reprogrammable spin wave band structure in Permalloy(10 nm)/Cu(5 nm)/Permalloy(30 nm) nanowire arrays of width w = 280 nm and inter-wire separation in the range from 80 to 280 nm. We found that, depending on the inter-wire separation, the anti-parallel configur...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40131-6
更新日期:2019-03-15 00:00:00
abstract::The estrogen signaling pathway has been reported to modulate prostate cancer (PCa) progression through the activity of estrogen receptors α and β (ERα and ERβ). Given that selective estrogen receptor modulators (SERMs) are used to treat breast cancer, ERs have been proposed as attractive therapeutic targets in PCa. Ho...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60844-3
更新日期:2020-03-04 00:00:00
abstract::Transparent heaters have attracted increasing attention for their usefulness in vehicle windows, outdoor displays, and periscopes. We present high performance transparent heaters based on Ag nanowires with electron beam irradiation. We obtained an Ag-nanowire thin film with 48 ohm/sq of sheet resistance and 88.8% (sub...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17716
更新日期:2015-12-07 00:00:00
abstract::Despite the tremendous progress of quantum cryptography, efficient quantum communication over long distances (≥ 1000 km) remains an outstanding challenge due to fiber attenuation and operation errors accumulated over the entire communication distance. Quantum repeaters (QRs), as a promising approach, can overcome both...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20463
更新日期:2016-02-15 00:00:00
abstract::The intrinsic functional architecture of the brain supports moment-to-moment maintenance of an internal model of the world. We hypothesized and found three interdependent architectural gradients underlying the organization of intrinsic functional connectivity within the human cerebral cortex. We used resting state fMR...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51793-7
更新日期:2019-11-04 00:00:00
abstract::The use of O-(2-[18F]fluoroethyl)-L-tyrosine ([18F]FET) as a positron emission tomography (PET) tracer for brain tumor imaging might have some limitations because of the relatively low affinity for the L-type amino acid transporter 1 (LAT1). To assess the stereospecificity and evaluate the influence of aromatic ring m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40013-x
更新日期:2019-02-27 00:00:00
abstract::Gene targeting in embryonic stem cells (ESCs) has become the principal technology for generating knockout models. Although numerous studies have predicted that the disruption of p53 leads to increased developmental anomalies and malignancies, most p53 knockout mice develop normally. Therefore, the role of p53 in anima...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00945
更新日期:2012-01-01 00:00:00
abstract::Tetrameric coiled-coil structures are present in many ion channels, often adjacent to a calmodulin (CaM) binding site, although the relationship between the two is not completely understood. Here we examine the dynamic properties of the ABCD domain located in the intracellular C-terminus of tetrameric, voltage-depende...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13811-4
更新日期:2017-10-18 00:00:00
abstract::Regime shift from grasslands to shrub-dominated landscapes occur worldwide driven by altered land-use and climate change, affecting landscape function, biodiversity, and productivity. Warming winter temperatures are a main driver of expansion of the native, evergreen shrub, Morella cerifera, in coastal landscapes. Shr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65161-3
更新日期:2020-05-19 00:00:00
abstract::The present study aimed to evaluate the accuracy and repeatability of morphological contour interpolation (MCI)-based semiautomatic segmentation method for volumetric measurements of bone grafts around dental implants. Three in vitro (one with a cylinder and two with a geometrically complex form) and four ex vivo mode...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71651-1
更新日期:2020-09-10 00:00:00
abstract::Endocannabinoids are small signaling lipids, with 2-arachidonoylglycerol (2-AG) implicated in modulating axonal growth and synaptic plasticity. The concept of short-range extracellular signaling by endocannabinoids is supported by the lack of trans-synaptic 2-AG signaling in mice lacking sn-1-diacylglycerol lipases (D...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02093
更新日期:2013-01-01 00:00:00
abstract::Soil moisture is an important variable for land-climate and hydrological interactions. To investigate emergent large-scale, long-term interactions between soil moisture and other key hydro-climatic variables (precipitation, actual evapotranspiration, runoff, temperature), we analyze monthly values and anomalies of the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61621-y
更新日期:2020-03-16 00:00:00
abstract::There is an increasing appreciation for the role of the human Y chromosome in phenotypic differences between the sexes in health and disease. Previous studies have shown that genetic variation within the Y chromosome is associated with cholesterol levels, which is an established risk factor for atherosclerosis, the un...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17165-9
更新日期:2017-12-01 00:00:00
abstract::Toll-like receptors (TLRs) play a key role in innate and adaptive immunity, protecting the host from viral pathogens. We studied the effect of TLR7 polymorphisms on disease susceptibility and progression of chronic hepatitis B (CHB) infection in Chinese adults. Blood samples were taken from 612 patients with confirmed...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12698-5
更新日期:2017-09-29 00:00:00
abstract::The heterogeneity of metabolic reactions leads to a non-uniform distribution of temperature in different parts of the living cell. The demand to study normal functioning and pathological abnormalities of cellular processes requires the development of new visualization methods. Previously, we have shown that the 35-kDa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45421-7
更新日期:2019-06-20 00:00:00
abstract::Aggregates of corundum crystals with skeletal to hopper morphology occur in pyroclastic rocks erupted from Cretaceous basaltic volcanoes on Mt Carmel, N. Israel. The rapid growth of the crystals trapped volumes of the parental Al2O3-supersaturated melt; phenocrysts of tistarite (Ti2O3) in the trapped melts indicate cr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79739-4
更新日期:2021-01-28 00:00:00
abstract::A role of gut-brain axis emerges in the pathophysiology of anorexia nervosa and maintaining adapted physical activity during refeeding remains discussed. We aimed to assess gastrointestinal protein metabolism and investigate the contribution of physical activity during refeeding in C57BL/6 mice with activity-based ano...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21887
更新日期:2016-02-24 00:00:00
abstract::The classical view of nerve growth factor (NGF) action in the nervous system is linked to its retrograde axonal transport. However, almost nothing is known on the trafficking properties of its unprocessed precursor proNGF, characterized by different and generally opposite biological functions with respect to its matur...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20272
更新日期:2016-02-01 00:00:00
abstract::We generated self-induced retinal ganglion cells (RGCs) with functional axons from human induced pluripotent stem cells. After development of the optic vesicle from the induced stem cell embryoid body in three-dimensional culture, conversion to two-dimensional culture, achieved by supplementation with BDNF, resulted i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08344
更新日期:2015-02-10 00:00:00
abstract::Water-soluble protein content (WSPC) is a critical factor in both soybean protein quality and functionality. However, the underlying genetic determinants are unclear. Here, we used 219 soybean accessions and 152 recombinant inbred lines genotyped with high-density markers and phenotyped in multi-environments to dissec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04685-7
更新日期:2017-07-11 00:00:00
abstract::A photovoltaic reactor was designed for artificial photosynthesis, based on the reactions involved in high energy hydrogen atoms, which were produced from water electrolysis. Water and CO2, under the conditions studied, were converted to oxalate (H2C2O4) and a polymer. This was the first time that the oxalates and oxa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03572
更新日期:2014-01-06 00:00:00
abstract::A major limitation hindering the widespread use of synthetic phages in medical and industrial settings is the lack of an efficient phage-engineering platform. Classical T4 phage engineering and several newly proposed methods are often inefficient and time consuming and consequently, only able to produce an inconsisten...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75426-6
更新日期:2020-10-26 00:00:00
abstract::SerpinB3 has been recently described as an early marker of liver carcinogenesis, but the potential mechanistic role of this serpin in tumor development is still poorly understood. Overexpression of Myc often correlates with more aggressive tumour forms, supporting its involvement in carcinogenesis. Yes-associated prot...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17701
更新日期:2015-12-04 00:00:00
abstract::Dichapetalum cymosum produces the toxic fluorinated metabolite, fluoroacetate, presumably as a defence mechanism. Given the rarity of fluorinated metabolites in nature, the biosynthetic origin and function of fluoroacetate have been of particular interest. However, the mechanism for fluorination in D. cymosum was neve...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77598-7
更新日期:2020-11-25 00:00:00