Abstract：
:The classical view of nerve growth factor (NGF) action in the nervous system is linked to its retrograde axonal transport. However, almost nothing is known on the trafficking properties of its unprocessed precursor proNGF, characterized by different and generally opposite biological functions with respect to its mature counterpart. Here we developed a strategy to fluorolabel both purified precursor and mature neurotrophins (NTs) with a controlled stoichiometry and insertion site. Using a single particle tracking approach, we characterized the axonal transport of proNGF versus mature NGF in living dorsal root ganglion neurons grown in compartmentalized microfluidic devices. We demonstrate that proNGF is retrogradely transported as NGF, but with a lower flux and a different distribution of numbers of neurotrophins per vesicle. Moreover, exploiting a dual-color labelling technique, we analysed the transport of both NT forms when simultaneously administered to the axon tips.
journal_name
Sci Repjournal_title
Scientific reportsauthors
De Nadai T,Marchetti L,Di Rienzo C,Calvello M,Signore G,Di Matteo P,Gobbo F,Turturro S,Meucci S,Viegi A,Beltram F,Luin S,Cattaneo Adoi
10.1038/srep20272subject
Has Abstractpub_date
2016-02-01 00:00:00pages
20272issn
2045-2322pii
srep20272journal_volume
6pub_type
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