Abstract：
:Keloid is the abnormal wound healing puzzled by the aggressive growth and high recurrence rate due to its unrevealed key pathogenic mechanism. MicroRNAs contribute to a series of biological processes including epithelial-mesenchymal transition (EMT) and cells stemness involved in fibrotic disease. Here, using microRNAs microarray analysis we found mir-21-5p was significantly up-regulated in keloid epidermis. To investigate the role of miR-21-5p in keloid pathogenesis, we transfected miR-21-5p mimic or inhibitor in keloid keratinocytes and examined the abilities of cell proliferation, apoptosis, migration and invasion, the expressions of EMT-related markers vimentin and E-cadherin and stem-like cells-associated markers CD44 and ALDH1, and the involvement of PTEN and the signaling of AKT and ERK. Our results demonstrated that up-regulation or knockdown of miR-21-5p significantly increased or decreased the migration, invasion and sphere-forming abilities of keloid keratinocytes, and the phenotype of EMT and cells stemness were enhanced or reduced as well. Furthermore, PTEN and p-AKT were shown to participate in the regulation of miR-21-5p on EMT phenotypes and stemness signatures of keloid keratinocytes, which might account for the invasion and recurrence of keloids. This molecular mechanism of miR-21-5p on keloid keratinocytes linked EMT with cells stemness and implicated novel therapeutic targets for keloids.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Yan L,Cao R,Liu Y,Wang L,Pan B,Lv X,Jiao H,Zhuang Q,Sun X,Xiao Rdoi
10.1038/srep28281subject
Has Abstractpub_date
2016-09-06 00:00:00pages
28281issn
2045-2322pii
srep28281journal_volume
6pub_type
杂志文章abstract：:In long-lived species, the age-, stage- and/or sex-dependent patterns of survival and reproduction determine the evolution of life history strategies, the shape of the reproductive value, and ultimately population dynamics. We evaluate the combined effects of age and sex in recruitment, breeder survival and breeding s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40204
更新日期：2017-01-11 00:00:00
abstract：:Hfq is a bacterial RNA binding protein that carries out several roles in genetic expression regulation, mainly at the post-transcriptional level. Previous studies have shown its importance in growth and virulence of bacteria. Here, we provide the direct observation of its ability to interact with membranes. This was e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11157-5
更新日期：2017-09-06 00:00:00
abstract：:Nitric oxide (NO)-induced apoptosis in neurons is an important cause of neurodegenerative disease in humans. The cold-inducible protein RBM3 mediates the protective effects of cooling on apoptosis induced by various insults. However, whether RBM3 protects neural cells from NO-induced apoptosis is unclear. This study a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41738
更新日期：2017-01-30 00:00:00
abstract：:The alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) is known to trigger the adaptive response by inducing the ada-regulon - consisting of three DNA repair enzymes Ada, AlkB, AlkA and the enigmatic AidB. We have applied custom designed tiling arrays to study transcriptional changes in Escherichia coli foll...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03053
更新日期：2013-10-25 00:00:00
abstract：:β1 integrin regulates the response of both normal and cancer cells to their local environment. Although mis-localised in prostate cancer, the role β1 integrin plays in prostate development and carcinogenesis remains unknown. To assess the role of β1 integrin in vivo, we conditionally deleted β1 integrin from prostate ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00526
更新日期：2012-01-01 00:00:00
abstract：:The repressive activity of ancestral histone-like proteins helps integrate transcription of foreign genes with discrepant AT content into existing regulatory networks. Our investigations indicate that the AT-rich discriminator region located between the -10 promoter element and the transcription start site of the regu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27780-9
更新日期：2018-06-21 00:00:00
abstract：:Antimicrobial stewardship programmes (ASP) are aimed at optimising antimicrobial utilization. However, only few studies have focused on paediatric intensive care units (PICU), where inappropriate antibiotic use occurs frequently. We assessed the effect and safety of a once weekly paediatric infectious disease (PID) wa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65671-0
更新日期：2020-06-01 00:00:00
abstract：:Alzheimer's disease (AD) is one of the most prevalent neurodegenerative diseases, yet current therapeutic treatments are inadequate due to a complex disease pathogenesis. The plant polyphenol apigenin has been shown to have anti-inflammatory and neuroprotective properties in a number of cell and animal models; however...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31450
更新日期：2016-08-12 00:00:00
abstract：:The arthropod-transmitted chikungunya virus (CHIKV) causes a flu-like disease that is characterized by incapacitating arthralgia. The re-emergence of CHIKV and the continual risk of new epidemics have reignited research in CHIKV pathogenesis. Virus-specific antibodies have been shown to control virus clearance, but an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20305-4
更新日期：2018-01-30 00:00:00
abstract：:Long-term daylight deprivation such as during the Antarctic winter has been shown to lead to delayed sleep timing and sleep fragmentation. We aimed at testing whether retinal sensitivity, sleep and circadian rest-activity will change during long-term daylight deprivation on two Antarctic bases (Concordia and Halley VI...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33450-7
更新日期：2018-11-01 00:00:00
abstract：:The structure and function of cellular membranes were sustained by redox-enzymes. We studied the interaction between the oxidative stress caused by excessive accumulation of ZnO-nanoparticles (ZnO-NPs) in plants and the role of redox-enzymes that can alleviate this stress. The crude callus extract from pomegranate, wh...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76664-4
更新日期：2020-11-12 00:00:00
abstract：:Singlet oxygen (1O2) is a type of reactive oxygen species involved in numerous physiological activities. We previously reported that 1O2-specific oxidation products are increased in patients with prediabetes, suggesting that measurement of 1O2 may be an important indicator of physiological and pathological conditions....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67155-7
更新日期：2020-06-30 00:00:00
abstract：:Obesity and depression are two common medical problems that pregnant women present with in antenatal care. Overweight and obesity at the beginning of the pregnancy, and excessive weight gain during pregnancy, are independent explanatory variables for fetal birthweight and independent risk factors for giving birth to a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42360-1
更新日期：2019-04-17 00:00:00
abstract：:Visual information can alter auditory perception. This is clearly illustrated by the well-known McGurk illusion, where an auditory/aba/ and a visual /aga/ are merged to the percept of 'ada'. It is less clear however whether such a change in perception may recalibrate subsequent perception. Here we asked whether the al...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32891
更新日期：2016-09-09 00:00:00
abstract：:To master linguistic communication, humans must acquire large vocabularies quickly and effortlessly. Efficient word learning might be facilitated by the ability to rapidly acquire novel word forms even outside the focus of attention, occurring within minutes of repetitive exposure and suggesting fast and automatic lex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34029-y
更新日期：2018-10-25 00:00:00
abstract：:Assembly of nanowires into ordered macroscopic structures with new functionalities has been a recent focus. In this Letter, we report a new route for ordering hydrophilic Ag nanowires with high aspect ratio by flowing through a glass capillary. The present glass capillary with well-defined silver nanowire films inside...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00987
更新日期：2012-01-01 00:00:00
abstract：:The effects of ocean acidification (OA) on the early recruitment of pteropods in the Scotia Sea, was investigated considering the process of spawning, quality of the spawned eggs and their capacity to develop. Maternal OA stress was induced on female pteropods (Limacina helicina antarctica) through exposure to present...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25752
更新日期：2016-05-16 00:00:00
abstract：:Diabetes is caused by the loss or dysfunction of insulin-secreting β-cells in the pancreas. β-cells reduce their mass and lose insulin-producing ability in vitro, likely due to insufficient cell-cell and cell-extracellular matrix (ECM) interactions as β-cells lose their native microenvironment. Herein, we built an ex-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02863
更新日期：2013-10-04 00:00:00
abstract：:We developed hybrid slot antenna structures for microbial sensing in the THz frequency range, where silver nanowires (AgNWs) were employed to increase the sensitivity. In order to fabricate the hybrid devices, we partially etched the AgNW in the slot antenna region, where we can expect the field enhancement effect at ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33617-2
更新日期：2018-10-19 00:00:00
abstract：:Regular drusen, an accumulation of material below the retinal pigment epithelium (RPE), have long been established as a hallmark early feature of nonneovascular age-related macular degeneration (AMD). Advances in imaging have expanded the phenotype of AMD to include another extracellular deposit, reticular pseudodruse...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66355-5
更新日期：2020-06-12 00:00:00
abstract：:Programmed cell death 1 (PD-1) monoclonal antibodies have been approved by regulatory agencies for the treatment of various types of cancer, and the mechanism involves the restoration of T cell functions. We report herein the X-ray crystal structure of a fully human monoclonal antibody mAb059c fragment antigen-binding...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54231-w
更新日期：2019-11-28 00:00:00
abstract：:Polymer light emitting diodes (PLEDs) using quantum dots (QDs) as emissive materials have received much attention as promising components for next-generation displays. Despite their outstanding properties, toxic and hazardous nature of QDs is a serious impediment to their use in future eco-friendly opto-electronic dev...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11032
更新日期：2015-06-11 00:00:00
abstract：:The green bacterial biosynthesis of lead sulfide nanocrystallites by L-cysteine-desulfurizing bacterium Lysinibacillus sphaericus SH72 was demonstrated in this work. Nanocrystals formed by this bacterial method were characterized using the mineralogical and morphological approaches. The results revealed that the micro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80450-7
更新日期：2021-01-13 00:00:00
abstract：:Gout/hyperuricemia is a common multifactorial disease having typical environmental risks. Recently, common dysfunctional variants of ABCG2, a urate exporter gene also known as BCRP, are revealed to be a major cause of gout/hyperuricemia. Here, we compared the influence of ABCG2 dysfunction on serum uric acid (SUA) lev...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05227
更新日期：2014-06-09 00:00:00
abstract：:This paper has been retracted at the request of the authors. ...
journal_title：Scientific reports
pub_type： 撤回出版物
doi：10.1038/s41598-018-31224-9
更新日期：2018-09-04 00:00:00
abstract：:EhV-ATPase is an ATP-driven Na+ pump in the eubacteria Enterococcus hirae (Eh). Here, we present the first entire structure of detergent-solubilized EhV-ATPase by single-particle cryo-electron microscopy (cryo-EM) using Zernike phase plate. The cryo-EM map dominantly showed one of three catalytic conformations in this...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33977-9
更新日期：2018-10-23 00:00:00
abstract：:Tuberculosis (TB) is a leading infectious cause of death globally. Drug treatment and vaccination, in particular with Bacillus Calmette-Guérin (BCG), remain the main strategies to control TB. With the emergence of drug resistance, it has been proposed that a combination of TB vaccination with pharmacological treatment...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41008-4
更新日期：2019-03-19 00:00:00
abstract：:Traditional diagnostic detection of chronic wasting disease (CWD) relies on immunodetection of misfolded CWD prion protein (PrP(CWD)) by western blotting, ELISA, or immunohistochemistry (IHC). These techniques require separate sample collections (frozen and fixed) which may result in discrepancies due to variation in ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25098
更新日期：2016-05-09 00:00:00
abstract：:Confirmatory tests for the diagnosis of brain death in addition to clinical findings may shorten observation time required in some countries and may add certainty to the diagnosis under specific circumstances. The practicability of Gadolinium-enhanced magnetic resonance angiography to confirm cerebral circulatory arre...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03659
更新日期：2014-01-13 00:00:00
abstract：:Lithium (Li) is the cornerstone maintenance treatment for bipolar disorders (BD), but response rates are highly variable. To date, no clinical or biological marker is available to reliably define eligibility criteria for a maintenance treatment with Li. We examined whether the prophylactic response to Li (assessed ret...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69073-0
更新日期：2020-07-22 00:00:00
