Abstract:
:Nuclear pore complexes (NPCs) fuse the inner and outer membranes of the nuclear envelope. They comprise hundreds of nucleoporins (Nups) that assemble into multiple subcomplexes and form large central channels for nucleocytoplasmic exchange1,2. How this architecture facilitates messenger RNA export, NPC biogenesis and turnover remains poorly understood. Here we combine in situ structural biology and integrative modelling with correlative light and electron microscopy and molecular perturbation to structurally analyse NPCs in intact Saccharomyces cerevisiae cells within the context of nuclear envelope remodelling. We find an in situ conformation and configuration of the Nup subcomplexes that was unexpected from the results of previous in vitro analyses. The configuration of the Nup159 complex appears critical to spatially accommodate its function as an mRNA export platform, and as a mediator of NPC turnover. The omega-shaped nuclear envelope herniae that accumulate in nup116Δ cells3 conceal partially assembled NPCs lacking multiple subcomplexes, including the Nup159 complex. Under conditions of starvation, herniae of a second type are formed that cytoplasmically expose NPCs. These results point to a model of NPC turnover in which NPC-containing vesicles bud off from the nuclear envelope before degradation by the autophagy machinery. Our study emphasizes the importance of investigating the structure-function relationship of macromolecular complexes in their cellular context.
journal_name
Naturejournal_title
Natureauthors
Allegretti M,Zimmerli CE,Rantos V,Wilfling F,Ronchi P,Fung HKH,Lee CW,Hagen W,Turoňová B,Karius K,Börmel M,Zhang X,Müller CW,Schwab Y,Mahamid J,Pfander B,Kosinski J,Beck Mdoi
10.1038/s41586-020-2670-5subject
Has Abstractpub_date
2020-10-01 00:00:00pages
796-800issue
7831eissn
0028-0836issn
1476-4687pii
10.1038/s41586-020-2670-5journal_volume
586pub_type
杂志文章相关文献
NATURE文献大全abstract::Although infection by HIV-1 has been implicated as the primary cause of AIDS and related disorders, cofactorial mechanisms may be involved in the pathogenesis of the disease. For example, several viruses commonly detected in AIDS patients and capable of transactivating the long terminal repeat of HIV-1, such as herpes...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/337370a0
更新日期:1989-01-26 00:00:00
abstract::Collections in early Middle Miocene deposits at Pasalar in Turkey have yielded a very rich fauna. Included in this are two hominoid species referred here to Sivapithecus darwini (Abel) 1902 and Ramapithecus wickeri (Leakey) 1962. These are both more primitive morphologically and earlier in time than other species of t...
journal_title:Nature
pub_type: 历史文章,杂志文章
doi:10.1038/268699a0
更新日期:1977-08-25 00:00:00
abstract::In mammals, the emergence of totipotency after fertilization involves extensive rearrangements of the spatial positioning of the genome1,2. However, the contribution of spatial genome organization to the regulation of developmental programs is unclear3. Here we generate high-resolution maps of genomic interactions wit...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-019-1233-0
更新日期:2019-05-01 00:00:00
abstract::Erythroid differentiation entails the biogenesis of a membrane skeleton, a network of proteins underlying and interacting with the plasma membrane, whose major constituent is the heterodimeric protein spectrin, composed of two structurally similar but distinct subunits, alpha (relative molecular mass (Mr) 240,000) and...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/321085a0
更新日期:1986-05-01 00:00:00
abstract::Current ideas about the dependence of neurons on target-derived growth factors were formulated on the basis of experiments involving neurons with projections to the periphery. Nerve growth factor (NGF) and recently identified members of the NGF family of neuronal growth factors, known as neurotrophins, are thought to ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/360753a0
更新日期:1992-12-24 00:00:00
abstract::Three environmental groups have filed suit against the National Institutes of Health in an attempt to block an experiment approved in April by the NIH Recombinant DNA Advisory Committee. The experiment, designed by researchers at the University of California, Berkeley, to enhance the frost resistance of crops, would ...
journal_title:Nature
pub_type: 新闻
doi:10.1038/305262a0
更新日期:1983-09-22 00:00:00
abstract::The nucleosome subunit of chromatin consists of DNA folded around a histone core as a 1.8-turn left-handed solenoid. The crystal structure of the nucleosome core particle revealed that it has a dyad symmetry axis and that the minor helix groove faces outwards from the protein core. Richmond et al. noticed that the pat...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/328554a0
更新日期:1987-08-06 00:00:00
abstract::Activity-dependent change in the efficacy of transmission is a basic feature of many excitatory synapses in the central nervous system. The best understood postsynaptic modification involves a change in responsiveness of AMPAR (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor)-mediated currents follo...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35013064
更新日期:2000-05-25 00:00:00
abstract::Brood parasitism, where a brood of the parasitic species is fostered by the parents of another species, is well known among birds. In most cases, such offspring show a complete reliance upon their host parents for food, protection and warmth until their independence. In other vertebrate groups, however, such total dep...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/323058a0
更新日期:1986-09-04 00:00:00
abstract::Groundwater drawn daily from shallow alluvial sands by millions of wells over large areas of south and southeast Asia exposes an estimated population of over a hundred million people to toxic levels of arsenic. Holocene aquifers are the source of widespread arsenic poisoning across the region. In contrast, Pleistocene...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature12444
更新日期:2013-09-12 00:00:00
abstract::The development of photolabile caged neurotransmitters has made possible the study of the split-second kinetics of receptor-ligand interactions. An instrument has now been developed for activating the neurotransmitter and measuring the neuron's response. ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/337583a0
更新日期:1989-02-09 00:00:00
abstract::The dopa analogue 6-fluorodopa (6-FD) used with positron emission tomography (PET) allows in vivo visualization of dopamine and its metabolites in nigrostriatal nerve endings. We have now found abnormal 6-FD scans in four subjects exposed to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). None had parkinsonism. T...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/317246a0
更新日期:1985-09-19 00:00:00
abstract::The Coffin-Lowry syndrome (CLS), an X-linked disorder, is characterized by severe psychomotor retardation, facial and digital dysmorphisms, and progressive skeletal deformations. Genetic linkage analysis mapped the CLS locus to an interval of 2-3 megabases at Xp22.2. The gene coding for Rsk-2, a member of the growth-f...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/384567a0
更新日期:1996-12-12 00:00:00
abstract::Signal sequences target proteins for secretion from cells or for integration into cell membranes. As nascent proteins emerge from the ribosome, signal sequences are recognized by the signal recognition particle (SRP), which subsequently associates with its receptor (SR). In this complex, the SRP and SR stimulate each ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02250
更新日期:2004-01-15 00:00:00
abstract::Calcitonin gene-related peptide (CGRP) is a widely expressed neuropeptide that has a major role in sensory neurotransmission. The CGRP receptor is a heterodimer of the calcitonin receptor-like receptor (CLR) class B G-protein-coupled receptor and a type 1 transmembrane domain protein, receptor activity-modifying prote...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-018-0535-y
更新日期:2018-09-01 00:00:00
abstract::Deep chlorophyll maxima (DCMs) are widespread in large parts of the world's oceans. These deep layers of high chlorophyll concentration reflect a compromise of phytoplankton growth exposed to two opposing resource gradients: light supplied from above and nutrients supplied from below. It is often argued that DCMs are ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature04245
更新日期:2006-01-19 00:00:00
abstract::Different classes of retroviruses have been shown to induce immunodeficiency diseases in various animal species. These animal models may provide an insight into our understanding of AIDS but, with the exception of one strain of feline leukaemia virus, the determinants of pathogenicity have not yet been mapped to these...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/338505a0
更新日期:1989-04-06 00:00:00
abstract::Sleep is one of the few major whole-organ phenomena for which no function and no underlying mechanism have been conclusively demonstrated. Sleep could result from global changes in the brain during wakefulness or it could be regulated by specific loci that recruit the rest of the brain into the electrical and metaboli...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature04811
更新日期:2006-06-08 00:00:00
abstract::Aquifex aeolicus was one of the earliest diverging, and is one of the most thermophilic, bacteria known. It can grow on hydrogen, oxygen, carbon dioxide, and mineral salts. The complex metabolic machinery needed for A. aeolicus to function as a chemolithoautotroph (an organism which uses an inorganic carbon source for...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/32831
更新日期:1998-03-26 00:00:00
abstract::Cutaneous melanoma is a type of cancer with an inherent potential for lymph node colonization, which is generally preceded by neolymphangiogenesis. However, sentinel lymph node removal does not necessarily extend the overall survival of patients with melanoma. Moreover, lymphatic vessels collapse and become dysfunctio...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature22977
更新日期:2017-06-28 00:00:00
abstract::The Encyclopedia of DNA Elements (ENCODE) project has established a genomic resource for mammalian development, profiling a diverse panel of mouse tissues at 8 developmental stages from 10.5 days after conception until birth, including transcriptomes, methylomes and chromatin states. Here we systematically examined th...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-2093-3
更新日期:2020-07-01 00:00:00
abstract::A variety of microbial communities and their genes (the microbiome) exist throughout the human body, with fundamental roles in human health and disease. The National Institutes of Health (NIH)-funded Human Microbiome Project Consortium has established a population-scale framework to develop metagenomic protocols, resu...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11209
更新日期:2012-06-13 00:00:00
abstract::Chick embryo fibroblasts (CEF) infected with avian sarcoma virus become rapidly transformed as a result of expression of the viral src gene in the form of a single polypeptide of molecular weight 60,000 (pp60src) with protein kinase activity and suggested preferential association with the plasma membrane. Studies with...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/287241a0
更新日期:1980-09-18 00:00:00
abstract::Muscle actin genes are the earliest yet described to show cell type-specific activation in amphibian embryos. Gene-specific probes show that alpha-skeletal and alpha-cardiac actin genes start to be transcribed simultaneously at the end of gastrulation, but only in those regions of the mesoderm that subsequently form e...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/311716a0
更新日期:1984-10-25 00:00:00
abstract::The effect of anthropogenic aerosols on cloud droplet concentrations and radiative properties is the source of one of the largest uncertainties in the radiative forcing of climate over the industrial period. This uncertainty affects our ability to estimate how sensitive the climate is to greenhouse gas emissions. Here...
journal_title:Nature
pub_type: 历史文章,杂志文章
doi:10.1038/nature12674
更新日期:2013-11-07 00:00:00
abstract::Obesity is now so common within the world's population that it is beginning to replace undernutrition and infectious diseases as the most significant contributor to ill health. In particular, obesity is associated with diabetes mellitus, coronary heart disease, certain forms of cancer, and sleep-breathing disorders. O...
journal_title:Nature
pub_type: 杂志文章,评审
doi:10.1038/35007508
更新日期:2000-04-06 00:00:00
abstract::Chromosomal replication machines contain coupled DNA polymerases that simultaneously replicate the leading and lagging strands. However, coupled replication presents a largely unrecognized topological problem. Because DNA polymerase must travel a helical path during synthesis, the physical connection between leading- ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11988
更新日期:2013-04-04 00:00:00
abstract::Understanding brain circuits begins with an appreciation of their component parts - the cells. Although GABAergic interneurons are a minority population within the brain, they are crucial for the control of inhibition. Determining the diversity of these interneurons has been a central goal of neurobiologists, but this...
journal_title:Nature
pub_type: 杂志文章,评审
doi:10.1038/nature12983
更新日期:2014-01-16 00:00:00
abstract::Many cellular functions involve multi-domain proteins, which are composed of structurally independent modules connected by flexible linkers. Although it is often well understood how a given domain recognizes a cognate oligonucleotide or peptide motif, the dynamic interaction of multiple domains in the recognition of t...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature10171
更新日期:2011-07-13 00:00:00
abstract::Visual transduction in vertebrate photoreceptors is thought to involve a diffusible internal transmitter which links photon absorption in disk membranes to the conductance decrease in the plasma membrane of the outer segment. The desensitisation of the photoresponse which occurs during and after illumination may also ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/283085a0
更新日期:1980-01-03 00:00:00