当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Dual SMAD inhibition and Wnt inhibition enable efficient and reproducible differentiations of induced pluripotent stem cells into retinal ganglion cells.

Dual SMAD inhibition and Wnt inhibition enable efficient and reproducible differentiations of induced pluripotent stem cells into retinal ganglion cells.

Abstract：

:Glaucoma is a group of progressive optic neuropathies that share common biological and clinical characteristics including irreversible changes to the optic nerve and visual field loss caused by the death of retinal ganglion cells (RGCs). The loss of RGCs manifests as characteristic cupping or optic nerve degeneration, resulting in visual field loss in patients with Glaucoma. Published studies on in vitro RGC differentiation from stem cells utilized classical RGC signaling pathways mimicking retinal development in vivo. Although many strategies allowed for the generation of RGCs, increased variability between experiments and lower yield hampered the cross comparison between individual lines and between experiments. To address this critical need, we developed a reproducible chemically defined in vitro methodology for generating retinal progenitor cell (RPC) populations from iPSCs, that are efficiently directed towards RGC lineage. Using this method, we reproducibly differentiated iPSCs into RGCs with greater than 80% purity, without any genetic modifications. We used small molecules and peptide modulators to inhibit BMP, TGF-β (SMAD), and canonical Wnt pathways that reduced variability between iPSC lines and yielded functional and mature iPSC-RGCs. Using CD90.2 antibody and Magnetic Activated Cell Sorter (MACS) technique, we successfully purified Thy-1 positive RGCs with nearly 95% purity.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Chavali VRM,Haider N,Rathi S,Vrathasha V,Alapati T,He J,Gill K,Nikonov R,Duong TT,McDougald DS,Nikonov S,O'Brien J,Mills JA

doi

10.1038/s41598-020-68811-8

subject

Has Abstract

pub_date

2020-07-16 00:00:00

pages

11828

issue

1

issn

2045-2322

pii

10.1038/s41598-020-68811-8

journal_volume

10

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Rational design of mutations that change the aggregation rate of a protein while maintaining its native structure and stability.

    abstract：:A wide range of human diseases is associated with mutations that, destabilizing proteins native state, promote their aggregation. However, the mechanisms leading from folded to aggregated states are still incompletely understood. To investigate these mechanisms, we used a combination of NMR spectroscopy and molecular ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep25559

    authors： Camilloni C,Sala BM,Sormanni P,Porcari R,Corazza A,De Rosa M,Zanini S,Barbiroli A,Esposito G,Bolognesi M,Bellotti V,Vendruscolo M,Ricagno S

    更新日期：2016-05-06 00:00:00

  • Tailoring of silica-based nanoporous pod by spermidine multi-activity.

    abstract：:Ubiquitous in nature, polyamines (PAs) are a class of low-molecular aliphatic amines critically involved in cell growth, survival and differentiation. The polycation behavior is validated as a successful strategy in delivery systems to enhance oligonucleotide loading and cellular uptake. In this study, the chemical fe...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-77957-4

    authors： Della Rosa G,Di Corato R,Carpi S,Polini B,Taurino A,Tedeschi L,Nieri P,Rinaldi R,Aloisi A

    更新日期：2020-12-03 00:00:00

  • Characterising a PDMS based 3D cell culturing microfluidic platform for screening chemotherapeutic drug cytotoxic activity.

    abstract：:Three-dimensional (3D) spheroidal cell cultures are now recognised as better models of cancers as compared to traditional cell cultures. However, established 3D cell culturing protocols and techniques are time-consuming, manually laborious and often expensive due to the excessive consumption of reagents. Microfluidics...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-72952-1

    authors： Khot MI,Levenstein MA,de Boer GN,Armstrong G,Maisey T,Svavarsdottir HS,Andrew H,Perry SL,Kapur N,Jayne DG

    更新日期：2020-09-28 00:00:00

  • Genuine antiplasticizing effect of water on a glass-former drug.

    abstract：:Water is the most important plasticizer of biological and organic hydrophilic materials, which generally exhibit enhanced mechanical softness and molecular mobility upon hydration. The enhancement of the molecular dynamics upon mixing with water, which in glass-forming systems implies a lower glass transition temperat...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-07643-5

    authors： Ruiz GN,Romanini M,Hauptmann A,Loerting T,Shalaev E,Tamarit JL,Pardo LC,Macovez R

    更新日期：2017-08-07 00:00:00

  • A plasmonic refractive index sensor with an ultrabroad dynamic sensing range.

    abstract：:Refractive index sensors based on surface plasmon resonance (SPR) promise to deliver high sensitivities. However, these sensitivities depend on the derivative of the monitored SPR parameters near resonance, so this dependency leads to a relatively narrow detection range for refractive index changes. Herein, we introdu...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-41353-4

    authors： Cheng YC,Chang YJ,Chuang YC,Huang BZ,Chen CC

    更新日期：2019-03-26 00:00:00

  • Postnatal development of the electrophysiological properties of somatostatin interneurons in the anterior cingulate cortex of mice.

    abstract：:Somatostatin (SST)-positive interneurons in the anterior cingulate cortex (ACC) play important roles in neuronal diseases, memory and cognitive functions. However, their development in the ACC remains unclear. Using postnatal day 3 (P3) to P45 GIN mice, we found that most of the intrinsic membrane properties of SST in...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep28137

    authors： Pan G,Yang JM,Hu XY,Li XM

    更新日期：2016-06-20 00:00:00

  • Quantitative Profiling of the Lymph Node Clearance Capacity.

    abstract：:Transport of tissue-derived lymphatic fluid and clearance by draining lymph nodes are pivotal for maintenance of fluid homeostasis in the body and for immune-surveillance of the self- and non-self-proteomes. Yet a quantitative analysis of nodal filtration of the tissue-derived proteome present in lymphatic fluid has n...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-29614-0

    authors： Clement CC,Wang W,Dzieciatkowska M,Cortese M,Hansen KC,Becerra A,Thangaswamy S,Nizamutdinova I,Moon JY,Stern LJ,Gashev AA,Zawieja D,Santambrogio L

    更新日期：2018-07-26 00:00:00

  • Rates and trends of childhood acute lymphoblastic leukaemia: an epidemiology study.

    abstract：:Acute lymphoblastic leukaemia (ALL) is the most common childhood cancer and has a high survival rate when properly managed. Prognosis is correlated with many factors such as age, gender, white blood cell (WBC) count, CD10, French-American-British (FAB) classification, and many others. Many of these factors are include...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-63528-0

    authors： Kakaje A,Alhalabi MM,Ghareeb A,Karam B,Mansour B,Zahra B,Hamdan O

    更新日期：2020-04-21 00:00:00

  • Reproductive resource partitioning in two sympatric Goniothalamus species (Annonaceae) from Borneo: floral biology, pollinator trapping and plant breeding system.

    abstract：:The floral phenology, pollination ecology and breeding systems of two sympatric early-divergent angiosperms, Goniothalamus tapisoides and G. suaveolens (Annonaceae) are compared. The flowers are protogynous and morphologically similar, with anthesis over 23-25 h. Both species are predominantly xenogamous and pollinate...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep35674

    authors： Lau JY,Pang CC,Ramsden L,Saunders RM

    更新日期：2016-10-21 00:00:00

  • Comparison of prognostic, clinical, and renal histopathological characteristics of overlapping idiopathic membranous nephropathy and IgA nephropathy versus idiopathic membranous nephropathy.

    abstract：:Overlapping idiopathic membranous nephropathy (IMN) and immunoglobulin A nephropathy (IgAN) is rare. This study aims to investigate the unique prognostic, clinical, and renal histopathological characteristics of IMN+IgAN. This retrospective observational study included 73 consecutive cases of IMN+IgAN and 425 cases of...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-11838-1

    authors： Chen X,Chen Y,Shi K,Lv Y,Tong H,Zhao G,Chen C,Chen B,Li D,Lu Z

    更新日期：2017-09-13 00:00:00

  • Minimal domain of bacterial phytochrome required for chromophore binding and fluorescence.

    abstract：:Fluorescent proteins (FP) are used to study various biological processes. Recently, a series of near-infrared (NIR) FPs based on bacterial phytochromes was developed. Finding ways to improve NIR FPs is becoming progressively important. By applying rational design and molecular evolution we have engineered R. palustris...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep18348

    authors： Rumyantsev KA,Shcherbakova DM,Zakharova NI,Emelyanov AV,Turoverov KK,Verkhusha VV

    更新日期：2015-12-18 00:00:00

  • A novel way to identify specific powdery mildew resistance genes in hybrid barley cultivars.

    abstract：:Powdery mildew, a common cereal disease caused by the fungus Blumeria graminis, is a major limiting factor of barley production and genetic resistance is the most appropriate protection against it. To aid the breeding of new cultivars and their marketing, resistance genes can be postulated in homogeneous accessions. A...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-75978-7

    authors： Dreiseitl A

    更新日期：2020-11-03 00:00:00

  • Nanoplastics can change the secondary structure of proteins.

    abstract：:Submillimetre-sized plastic particles (microplastics and nanoplastics) of waste origin in the environment have been repeatedly suggested in recent years to have severe impact on living organisms. While the uptake of these materials has been unequivocally evidenced for animals, so far no adverse effects have been obser...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-52495-w

    authors： Hollóczki O,Gehrke S

    更新日期：2019-11-05 00:00:00

  • Fast adaptation of tropical diatoms to increased warming with trade-offs.

    abstract：:Ocean warming with climate change is forcing marine organisms to shift their distributions polewards and phenology. In warm tropical seas, evolutionary adaptation by local species to warming will be crucial to avoid predicted desertification and reduction in diversity. However, little is known about the adaptation of ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-36091-y

    authors： Jin P,Agustí S

    更新日期：2018-12-11 00:00:00

  • Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib.

    abstract：:Species generalization in the profound, modality-specific effects of Hedgehog pathway inhibition (HPI) in taste organ homeostasis and sensation is shown. With the HPI, cancer drug sonidegib, we demonstrate that the rat taste system, in addition to mouse, is regulated by Hedgehog signaling. After sonidegib treatment fo...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-34399-3

    authors： Kumari A,Yokota Y,Li L,Bradley RM,Mistretta CM

    更新日期：2018-11-01 00:00:00

  • Controlled electron injection facilitated by nanoparticles for laser wakefield acceleration.

    abstract：:We propose a novel injection scheme for laser-driven wakefield acceleration in which controllable localized electron injection is obtained by inserting nanoparticles into a plasma medium. The nanoparticles provide a very confined electric field that triggers localized electron injection where nonlinear plasma waves ar...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-34998-0

    authors： Cho MH,Pathak VB,Kim HT,Nam CH

    更新日期：2018-11-16 00:00:00

  • Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing.

    abstract：:We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find potential circulating mRNA markers for colorectal adenoma. Results showed t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-49445-x

    authors： Xue VW,Cheung MT,Chan PT,Luk LLY,Lee VH,Au TC,Yu AC,Cho WCS,Tsang HFA,Chan AK,Wong SCC

    更新日期：2019-09-10 00:00:00

  • Transcription factor ZNF148 is a negative regulator of human muscle differentiation.

    abstract：:Muscle differentiation is a complex process in which muscle progenitor cells undergo determination and eventually cellular fusion. This process is heavily regulated by such master transcription factors as MYOD and members of the MEF2 family. Here, we show that the transcription factor ZNF148 plays a direct role in hum...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-08267-5

    authors： Bakke J,Wright WC,Zamora AE,Ong SS,Wang YM,Hoyer JD,Brewer CT,Thomas PG,Chen T

    更新日期：2017-08-15 00:00:00

  • Enduring rules of care within pairs - how blue tit parents resume provisioning behaviour after experimental disturbance.

    abstract：:Sexual conflict over parental investment can result in suboptimal reproductive output. A recent hypothesis suggests that equality in investment, and hence conflict resolution, may be reached via coordination of parental activities like alternating nest visits. However, how robust patterns of care within couples are ag...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-39139-9

    authors： Iserbyt A,Griffioen M,Eens M,Müller W

    更新日期：2019-02-26 00:00:00

  • Resveratrol Pretreatment Attenuates Concanavalin A-induced Hepatitis through Reverse of Aberration in the Immune Response and Regenerative Capacity in Aged Mice.

    abstract：:Loss of regenerative capacity plays a critical role in age-related autoimmune hepatitis. Evidence implicates SIRT1 and p66shc in cell senescence, apoptosis, oxidative stress, and proliferation. This study investigated the effect of resveratrol on concanavalin A (Con A)-induced hepatitis in aged mice and the roles of S...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-02881-z

    authors： Huang TH,Chen CC,Liu HM,Lee TY,Shieh SH

    更新日期：2017-06-02 00:00:00

  • Decoding the dynamic representation of musical pitch from human brain activity.

    abstract：:In music, the perception of pitch is governed largely by its tonal function given the preceding harmonic structure of the music. While behavioral research has advanced our understanding of the perceptual representation of musical pitch, relatively little is known about its representational structure in the brain. Usin...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-19222-3

    authors： Sankaran N,Thompson WF,Carlile S,Carlson TA

    更新日期：2018-01-16 00:00:00

  • An engineered opsin monomer scrambles phospholipids.

    abstract：:The G protein-coupled receptor opsin is a phospholipid scramblase that facilitates rapid transbilayer phospholipid exchange in liposomes. The mechanism by which opsin scrambles lipids is unknown. It has been proposed that lipid translocation may occur at protein-protein interfaces of opsin dimers. To test this possibi...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-16842-z

    authors： Pandey K,Ploier B,Goren MA,Levitz J,Khelashvili G,Menon AK

    更新日期：2017-12-01 00:00:00

  • Genetic diversity and population structure of a mini-core subset from the world cowpea (Vigna unguiculata (L.) Walp.) germplasm collection.

    abstract：:The International Institute of Tropical Agriculture maintains the world's largest collection of cowpea germplasm of over 15,000 accessions. A sub-set of 298 lines from the loosely composed mini core collection of 370 landraces were genotyped based on genotyping by sequencing (GBS). Ward's minimum variance hierarchical...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-34555-9

    authors： Fatokun C,Girma G,Abberton M,Gedil M,Unachukwu N,Oyatomi O,Yusuf M,Rabbi I,Boukar O

    更新日期：2018-10-30 00:00:00

  • A functional SUMO-motif in the active site of PIM1 promotes its degradation via RNF4, and stimulates protein kinase activity.

    abstract：:The PIM1 serine/threonine protein kinase mediates growth factor and survival signalling, and cooperates potently with c-MYC during tumorigenesis. PIM1 is overexpressed in many human cancers and is a promising target for drug development. PIM1 levels are regulated mainly through cytokine-induced transcription and prote...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-03775-w

    authors： Iyer RS,Chatham L,Sleigh R,Meek DW

    更新日期：2017-06-15 00:00:00

  • Antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms.

    abstract：:This study investigated the efficacy of a biphasic synthetic β-tricalcium phosphate/calcium sulfate (β-TCP/CS) bone graft substitute for compatibility with vancomycin (V) in combination with tobramycin (T) or gentamicin (G) evidenced by the duration of potency and the prevention and killing efficacies of P. aeruginosa...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-80764-6

    authors： Jiang N,Dusane DH,Brooks JR,Delury CP,Aiken SS,Laycock PA,Stoodley P

    更新日期：2021-01-14 00:00:00

  • Definition of fractal topography to essential understanding of scale-invariance.

    abstract：:Fractal behavior is scale-invariant and widely characterized by fractal dimension. However, the cor-respondence between them is that fractal behavior uniquely determines a fractal dimension while a fractal dimension can be related to many possible fractal behaviors. Therefore, fractal behavior is independent of the fr...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep46672

    authors： Jin Y,Wu Y,Li H,Zhao M,Pan J

    更新日期：2017-04-24 00:00:00

  • Plasma prolactin and breast cancer risk: a meta- analysis.

    abstract：:Breast cancer is the most common cancer among women, and its incidence is on a constant rise. Previous studies suggest that higher levels of plasma prolactin are associated with escalated risk of breast cancer, however, these results are contradictory and inconclusive. PubMed and Medline were used to search and identi...

    journal_title：Scientific reports

    pub_type： 杂志文章,meta分析

    doi：10.1038/srep25998

    authors： Wang M,Wu X,Chai F,Zhang Y,Jiang J

    更新日期：2016-05-17 00:00:00

  • Squeezed-light-driven force detection with an optomechanical cavity in a Mach-Zehnder interferometer.

    abstract：:We analyze the performance of a force detector based on balanced measurements with a Mach-Zehnder interferometer incorporating a standard optomechanical cavity. The system is driven by a coherent superposition of coherent light and squeezed vacuum field, providing quantum correlation along with optical coherence in or...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-74629-1

    authors： Lee CW,Lee JH,Seok H

    更新日期：2020-10-15 00:00:00

  • Impact of floral characters, pollen limitation, and pollinator visitation on pollination success in different populations of Caragana korshinskii Kom.

    abstract：:Caragana korshinskii Kom. has a significant function in desert-grassland revegetation in arid regions. Plant reproduction in arid regions can be restricted due to inadequate pollen receipt and reduced pollen transfer. An assessment of pollination success as a result of pollen limitation and pollinator visitation in va...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-46271-z

    authors： Chen M,Zhao XY

    更新日期：2019-07-05 00:00:00

  • Gene-based association analysis identifies 190 genes affecting neuroticism.

    abstract：:Neuroticism is a personality trait, which is an important risk factor for psychiatric disorders. Recent genome-wide studies reported about 600 genes potentially influencing neuroticism. Little is known about the mechanisms of their action. Here, we aimed to conduct a more detailed analysis of genes that can regulate t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-021-82123-5

    authors： Belonogova NM,Zorkoltseva IV,Tsepilov YA,Axenovich TI

    更新日期：2021-01-28 00:00:00