当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > A functional SUMO-motif in the active site of PIM1 promotes its degradation via RNF4, and stimulates protein kinase activity.

A functional SUMO-motif in the active site of PIM1 promotes its degradation via RNF4, and stimulates protein kinase activity.

Abstract：

:The PIM1 serine/threonine protein kinase mediates growth factor and survival signalling, and cooperates potently with c-MYC during tumorigenesis. PIM1 is overexpressed in many human cancers and is a promising target for drug development. PIM1 levels are regulated mainly through cytokine-induced transcription and protein degradation, but mechanisms regulating its activity and levels remain largely unexplored. Here, we show that PIM1 is modified in vitro and in cultured cells by the Small ubiquitin-like modifier (SUMO) on two independent sites: K169, within a consensus SUMOylation motif (IK169DE171) in the active site of PIM1, and also at a second promiscuous site. Alanine substitution of E171 (within the consensus motif) abolished SUMOylation, significantly increased the half-life of PIM1, and markedly reduced its ubiquitylation. Mechanistically, SUMOylation promoted ubiquitin-mediated degradation of PIM1 via recruitment of the SUMO-targeted ubiquitin ligase, RNF4. Additionally, SUMOylated PIM1 showed enhanced protein kinase activity in vitro. Interestingly, the E171A mutant was active in vitro but displayed altered substrate specificity in cultured cells, consistent with the idea that SUMOylation may govern PIM1 substrate specificity under certain contexts. Taken together, these data demonstrate that the protein kinase activity and levels of PIM1 can be regulated by a covalent post-translational modification.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Iyer RS,Chatham L,Sleigh R,Meek DW

doi

10.1038/s41598-017-03775-w

subject

Has Abstract

pub_date

2017-06-15 00:00:00

pages

3598

issue

1

issn

2045-2322

pii

10.1038/s41598-017-03775-w

journal_volume

7

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Adventitial lymphatic capillary expansion impacts on plaque T cell accumulation in atherosclerosis.

    abstract：:During plaque progression, inflammatory cells progressively accumulate in the adventitia, paralleled by an increased presence of leaky vasa vasorum. We here show that next to vasa vasorum, also the adventitial lymphatic capillary bed is expanding during plaque development in humans and mouse models of atherosclerosis....

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep45263

    authors： Rademakers T,van der Vorst EP,Daissormont IT,Otten JJ,Theodorou K,Theelen TL,Gijbels M,Anisimov A,Nurmi H,Lindeman JH,Schober A,Heeneman S,Alitalo K,Biessen EA

    更新日期：2017-03-28 00:00:00

  • Continuous-wave phase-matched molecular optical modulator.

    abstract：:In optical modulation, the highest available modulation rate is basically limited to the GHz frequency range at best. This is because optical modulation is often performed using electro-optic or acousto-optic effects that require application of an external signal to solid-state nonlinear optical materials. Here we des...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep20908

    authors： Zaitsu S,Izaki H,Tsuchiya T,Imasaka T

    更新日期：2016-02-18 00:00:00

  • Novel concept for neutron detection: proportional counter filled with 10B nanoparticle aerosol.

    abstract：:The high neutron detection efficiency, good gamma-ray discrimination and non-toxicity of 3He made of proportional counters filled with this gas the obvious choice for neutron detection, particularly in radiation portal monitors (RPM), used to control the illicit transport of nuclear material, of which neutron detector...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep41699

    authors： Amaro FD,Monteiro CM,Dos Santos JM,Antognini A

    更新日期：2017-02-09 00:00:00

  • Global view of cognate kinase activation by the human pyruvate dehydrogenase complex.

    abstract：:The human pyruvate dehydrogenase complex (PDC) comprises four multidomain components, E1, E3, E2 and an E3-binding protein (E3BP), the latter two forming the core as E2·E3BP sub-complex. Pyruvate flux through PDC is regulated via phosphorylation (inactivation) at E1 by four PDC kinases (PDKs), and reactivation by two ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep42760

    authors： Guevara EL,Yang L,Birkaya B,Zhou J,Nemeria NS,Patel MS,Jordan F

    更新日期：2017-02-23 00:00:00

  • A New Route for High-Purity Organic Materials: High-Pressure-Ramp-Induced Ultrafast Polymerization of 2-(Hydroxyethyl)Methacrylate.

    abstract：:The synthesis of highly biocompatible polymers is important for modern biotechnologies and medicine. Here, we report a unique process based on a two-step high-pressure ramp (HPR) for the ultrafast and efficient bulk polymerization of 2-(hydroxyethyl)methacrylate (HEMA) at room temperature without photo- and thermal ac...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep18244

    authors： Evlyukhin E,Museur L,Traore M,Perruchot C,Zerr A,Kanaev A

    更新日期：2015-12-16 00:00:00

  • Link between concentrations of sediment flux and deep crustal processes beneath the European Alps.

    abstract：:Large sediment fluxes from mountain belts have the potential to cause megafans to prograde into the neighbouring sedimentary basins. These mechanisms have been documented based from numerical modelling and stratigraphic records. However, little attention has been focused on inferring temporal changes in the concentrat...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-17182-8

    authors： Garefalakis P,Schlunegger F

    更新日期：2018-01-09 00:00:00

  • A combination approach to treating fungal infections.

    abstract：:Azoles are antifungal drugs used to treat fungal infections such as candidiasis in humans. Their extensive use has led to the emergence of drug resistance, complicating antifungal therapy for yeast infections in critically ill patients. Combination therapy has become popular in clinical practice as a potential strateg...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep17070

    authors： Shrestha SK,Fosso MY,Garneau-Tsodikova S

    更新日期：2015-11-23 00:00:00

  • Loss of the six3/6 controlling pathways might have resulted in pinhole-eye evolution in Nautilus.

    abstract：:Coleoid cephalopods have an elaborate camera eye whereas nautiloids have primitive pinhole eye without lens and cornea. The Nautilus pinhole eye provides a unique example to explore the module of lens formation and its evolutionary mechanism. Here, we conducted an RNA-seq study of developing eyes of Nautilus and pygmy...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep01432

    authors： Ogura A,Yoshida MA,Moritaki T,Okuda Y,Sese J,Shimizu KK,Sousounis K,Tsonis PA

    更新日期：2013-01-01 00:00:00

  • Organic nitrogen uptake is a significant contributor to nitrogen economy of subtropical epiphytic bryophytes.

    abstract：:Without any root contact with the soil, epiphytic bryophytes must experience and explore poor, patchy, and heterogeneous habitats; while, the nitrogen (N) uptake and use strategies of these organisms remain uncharacterized, which obscures their roles in the N cycle. To investigate the N sources, N preferences, and res...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep30408

    authors： Song L,Lu HZ,Xu XL,Li S,Shi XM,Chen X,Wu Y,Huang JB,Chen Q,Liu S,Wu CS,Liu WY

    更新日期：2016-07-27 00:00:00

  • A review on imaging techniques and quantitative measurements for dynamic imaging of cerebral aneurysm pulsations.

    abstract：:Measurement of intracranial aneurysm wall motion may refine the current rupture risk estimation. A golden standard for measuring aneurysm pulsation is lacking. The aim is to evaluate magnitudes of aneurysm pulsation as published in current literature. Embase and PubMed were searched for publications containing quantit...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-021-81753-z

    authors： Stam LB,Aquarius R,de Jong GA,Slump CH,Meijer FJA,Boogaarts HD

    更新日期：2021-01-26 00:00:00

  • Synergistic effects of tung oil and heat treatment on physicochemical properties of bamboo materials.

    abstract：:The search for green and sustainable modification method to produce durable bamboo materials remains a challenge in industry. Here, heat treatment in tung oil at 100-200 °C was employed to modify bamboo materials. Oil permeation and distribution in the structure of bamboo samples during heat treatment were explored. T...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-49240-8

    authors： Tang T,Zhang B,Liu X,Wang W,Chen X,Fei B

    更新日期：2019-09-06 00:00:00

  • Integrated genomic approaches identify major pathways and upstream regulators in late onset Alzheimer's disease.

    abstract：:Previous studies have evaluated gene expression in Alzheimer's disease (AD) brains to identify mechanistic processes, but have been limited by the size of the datasets studied. Here we have implemented a novel meta-analysis approach to identify differentially expressed genes (DEGs) in published datasets comprising 450...

    journal_title：Scientific reports

    pub_type： 杂志文章,meta分析

    doi：10.1038/srep12393

    authors： Li X,Long J,He T,Belshaw R,Scott J

    更新日期：2015-07-23 00:00:00

  • Endocrine disrupter chemicals affect the humoral antimicrobial activities of gilthead seabream males even upon the cease of the exposure.

    abstract：:17α-ethynilestradiol (EE2) and tamoxifen (Tmx) are pollutants world-wide distributed in aquatic environments. Gilthead seabream, Sparus aurata L., is highlighted as a species model of intensively culture in anthropogenic disturbed environments. The effects of these pollutants on gilthead seabream reproduction and some...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-64522-2

    authors： Valero Y,López-Cánovas AE,Rodenas MC,Cabas I,García-Hernández P,Arizcun M,García-Ayala A,Chaves-Pozo E

    更新日期：2020-05-14 00:00:00

  • The metabolic footprint of compromised insulin sensitivity under fasting and hyperinsulinemic-euglycemic clamp conditions in an Arab population.

    abstract：:Metabolic pathways that are corrupted at early stages of insulin resistance (IR) remain elusive. This study investigates changes in body metabolism in clinically healthy and otherwise asymptomatic subjects that may become apparent already under compromised insulin sensitivity (IS) and prior to IR. 47 clinically health...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-73723-8

    authors： Halama A,Suleiman NN,Kulinski M,Bettahi I,Hassoun S,Alkasem M,Abdalhakam I,Iskandarani A,Samra TA,Atkin SL,Suhre K,Abou-Samra AB

    更新日期：2020-10-13 00:00:00

  • Open-label placebos reduce test anxiety and improve self-management skills: A randomized-controlled trial.

    abstract：:Test anxiety is a condition in which people experience extreme distress and anxiety before and in test situations. It affects up to 40 percent of all students. Conventional treatment includes both medication and psychotherapy, but studies also demonstrated that placebos affect anxiety symptoms. Although in the traditi...

    journal_title：Scientific reports

    pub_type： 杂志文章,随机对照试验

    doi：10.1038/s41598-019-49466-6

    authors： Schaefer M,Denke C,Harke R,Olk N,Erkovan M,Enge S

    更新日期：2019-09-16 00:00:00

  • The feasibility and stability of large complex biological networks: a random matrix approach.

    abstract：:In the 70's, Robert May demonstrated that complexity creates instability in generic models of ecological networks having random interaction matrices A. Similar random matrix models have since been applied in many disciplines. Central to assessing stability is the "circular law" since it describes the eigenvalue distri...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-26486-2

    authors： Stone L

    更新日期：2018-05-29 00:00:00

  • Temozolomide suppresses MYC via activation of TAp63 to inhibit progression of human glioblastoma.

    abstract：:Glioblastoma multiforme (GBM) is a highly invasive and chemoradioresistant brain malignancy. Temozolomide (TMZ), a DNA-alkylating agent, is effective against GBM and has become the standard first-line drug. However, the mechanism by which TMZ regulates the progression of GBM remains elusive. Here, we demonstrate that ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep01160

    authors： Yamaki T,Suenaga Y,Iuchi T,Alagu J,Takatori A,Itami M,Araki A,Ohira M,Inoue M,Kageyama H,Yokoi S,Saeki N,Nakagawara A

    更新日期：2013-01-01 00:00:00

  • Magnetic-assisted triboelectric nanogenerators as self-powered visualized omnidirectional tilt sensing system.

    abstract：:The triboelectric nanogenerator (TENG) is a promising device in energy harvesting and self-powered sensing. In this work, we demonstrate a magnetic-assisted TENG, utilizing the magnetic force for electric generation. Maximum power density of 541.1 mW/m(2) is obtained at 16.67 MΩ for the triboelectric part, while the e...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep04811

    authors： Han M,Zhang XS,Sun X,Meng B,Liu W,Zhang H

    更新日期：2014-04-28 00:00:00

  • Functional characterization of retinal ganglion cells using tailored nonlinear modeling.

    abstract：:The mammalian retina encodes the visual world in action potentials generated by 20-50 functionally and anatomically-distinct types of retinal ganglion cell (RGC). Individual RGC types receive synaptic input from distinct presynaptic circuits; therefore, their responsiveness to specific features in the visual scene ari...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-45048-8

    authors： Shi Q,Gupta P,Boukhvalova AK,Singer JH,Butts DA

    更新日期：2019-06-18 00:00:00

  • Graphene based widely-tunable and singly-polarized pulse generation with random fiber lasers.

    abstract：:Pulse generation often requires a stabilized cavity and its corresponding mode structure for initial phase-locking. Contrastingly, modeless cavity-free random lasers provide new possibilities for high quantum efficiency lasing that could potentially be widely tunable spectrally and temporally. Pulse generation in rand...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep18526

    authors： Yao BC,Rao YJ,Wang ZN,Wu Y,Zhou JH,Wu H,Fan MQ,Cao XL,Zhang WL,Chen YF,Li YR,Churkin D,Turitsyn S,Wong CW

    更新日期：2015-12-21 00:00:00

  • Fabrication of laser printed microfluidic paper-based analytical devices (LP-µPADs) for point-of-care applications.

    abstract：:Microfluidic paper-based analytical devices (µPADs) have provided a breakthrough in portable and low-cost point-of-care diagnostics. Despite their significant scope, the complexity of fabrication and reliance on expensive and sophisticated tools, have limited their outreach and possibility of commercialization. Herein...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-44455-1

    authors： Ghosh R,Gopalakrishnan S,Savitha R,Renganathan T,Pushpavanam S

    更新日期：2019-05-27 00:00:00

  • Immune Response of A Novel ATR-AP205-001 Conjugate Anti-hypertensive Vaccine.

    abstract：:We developed a virus-like particle (VLP)-based therapeutic vaccine against angiotensin II receptor type 1, ATR-AP205-001, which could significantly reduce the blood pressure and protect target organs of hypertensive animals. In this study, we focused on the immunological effect and safety of the VLP-based vaccine. By ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-12996-y

    authors： Hu X,Deng Y,Chen X,Zhou Y,Zhang H,Wu H,Yang S,Chen F,Zhou Z,Wang M,Qiu Z,Liao Y

    更新日期：2017-10-03 00:00:00

  • Clinical efficacy of Stereotactic Body Radiation Therapy (SBRT) for adrenal gland metastases: A multi-center retrospective study from China.

    abstract：:To evaluate the efficacy and safety of CyberKnife Stereotactic Body Radiation Therapy (SBRT) in the treatment of adrenal gland metastases (AGM), we designed a large-scale multicenter retrospective study to report the safety and efficacy of SBRT for inoperable AGM. In this study, 75 (61 males, 14 females) patients with...

    journal_title：Scientific reports

    pub_type： 杂志文章,多中心研究

    doi：10.1038/s41598-020-64770-2

    authors： Zhao X,Zhu X,Zhuang H,Guo X,Song Y,Ju X,Wang P,Yuan Z,Zhang H

    更新日期：2020-05-12 00:00:00

  • Dynamic contact networks of patients and MRSA spread in hospitals.

    abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is a difficult-to-treat infection. Increasing efforts have been taken to mitigate the epidemics and to avoid potential outbreaks in low endemic settings. Understanding the population dynamics of MRSA is essential to identify the causal mechanisms driving the epidemics...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-66270-9

    authors： Rocha LEC,Singh V,Esch M,Lenaerts T,Liljeros F,Thorson A

    更新日期：2020-06-09 00:00:00

  • Seismic, satellite, and site observations of internal solitary waves in the NE South China Sea.

    abstract：:Internal solitary waves (ISWs) in the NE South China Sea (SCS) are tidally generated at the Luzon Strait. Their propagation, evolution, and dissipation processes involve numerous issues still poorly understood. Here, a novel method of seismic oceanography capable of capturing oceanic finescale structures is used to st...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep05374

    authors： Tang Q,Wang C,Wang D,Pawlowicz R

    更新日期：2014-06-20 00:00:00

  • Key role of 15-LO/15-HETE in angiogenesis and functional recovery in later stages of post-stroke mice.

    abstract：:This study sought to clarify the effects of 15-lipoxygenase/15-hydroxyeicosatetraenoic acid in angiogenesis and neurological functional recovery after cerebral ischaemic stroke in mice. In vivo, we performed behavioural tests to determine functional recovery after stroke. Double immunofluorescence staining of CD31 and...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep46698

    authors： Wang D,Liu Y,Chen L,Li P,Qu Y,Zhu Y,Zhu Y

    更新日期：2017-04-24 00:00:00

  • Ultraviolet protection of Bacillus thuringiensis through microencapsulation with Pickering emulsion method.

    abstract：:An encapsulated formulation of Bacillus thuringiensis (Bt) was produced by the Pickering emulsion technique to improve its activity and stability under UV-A radiation. In this technique latex particles, GO nanosheets, olive oil, ethanol, and water were used to encapsulate Bt in colloidosomes. The protective efficacy o...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-77721-8

    authors： Jalali E,Maghsoudi S,Noroozian E

    更新日期：2020-11-26 00:00:00

  • Influence of leaf vein density and thickness on hydraulic conductance and photosynthesis in rice (Oryza sativa L.) during water stress.

    abstract：:The leaf venation architecture is an ideal, highly structured and efficient irrigation system in plant leaves. Leaf vein density (LVD) and vein thickness are the two major properties of this system. Leaf laminae carry out photosynthesis to harvest the maximum biological yield. It is still unknown whether the LVD and/o...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep36894

    authors： Tabassum MA,Zhu G,Hafeez A,Wahid MA,Shaban M,Li Y

    更新日期：2016-11-16 00:00:00

  • Probing the Inhibition of Microtubule Affinity Regulating Kinase 4 by N-Substituted Acridones.

    abstract：:Microtubule affinity regulating kinase 4 (MARK4) becomes a unique anti-cancer drug target as its overexpression is responsible for different types of cancers. In quest of novel, effective MARK4 inhibitors, some acridone derivatives were synthesized, characterized and evaluated for inhibitory activity against human MAR...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-38217-8

    authors： Voura M,Khan P,Thysiadis S,Katsamakas S,Queen A,Hasan GM,Ali S,Sarli V,Hassan MI

    更新日期：2019-02-08 00:00:00

  • A Genome-wide Study of "Non-3UTR" Polyadenylation Sites in Arabidopsis thaliana.

    abstract：:Alternative polyadenylation has been recognized as a key contributor of gene expression regulation by generating different transcript isoforms with altered 3' ends. Although polyadenylation is well known for marking the end of a 3' UTR, an increasing number of studies have reported previously less-addressed polyadenyl...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep28060

    authors： Guo C,Spinelli M,Liu M,Li QQ,Liang C

    更新日期：2016-06-15 00:00:00