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Characterising a PDMS based 3D cell culturing microfluidic platform for screening chemotherapeutic drug cytotoxic activity.

Abstract：

:Three-dimensional (3D) spheroidal cell cultures are now recognised as better models of cancers as compared to traditional cell cultures. However, established 3D cell culturing protocols and techniques are time-consuming, manually laborious and often expensive due to the excessive consumption of reagents. Microfluidics allows for traditional laboratory-based biological experiments to be scaled down into miniature custom fabricated devices, where cost-effective experiments can be performed through the manipulation and flow of small volumes of fluid. In this study, we characterise a 3D cell culturing microfluidic device fabricated from a 3D printed master. HT29 cells were seeded into the device and 3D spheroids were generated and cultured through the perfusion of cell media. Spheroids were treated with 5-Fluorouracil for five days through continuous perfusion and cell viability was analysed on-chip at different time points using fluorescence microscopy and Lactate dehydrogenase (LDH) assay on the supernatant. Increasing cell death was observed in the HT29 spheroids over the five-day period. The 3D cell culturing microfluidic device described in this study, permits on-chip anti-cancer treatment and viability analysis, and forms the basis of an effective platform for the high-throughput screening of anti-cancer drugs in 3D tumour spheroids.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Khot MI,Levenstein MA,de Boer GN,Armstrong G,Maisey T,Svavarsdottir HS,Andrew H,Perry SL,Kapur N,Jayne DG

doi

10.1038/s41598-020-72952-1

subject

Has Abstract

pub_date

2020-09-28 00:00:00

pages

15915

issue

1

issn

2045-2322

pii

10.1038/s41598-020-72952-1

journal_volume

10

pub_type

杂志文章

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