当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Rational design of mutations that change the aggregation rate of a protein while maintaining its native structure and stability.

Rational design of mutations that change the aggregation rate of a protein while maintaining its native structure and stability.

Abstract：

:A wide range of human diseases is associated with mutations that, destabilizing proteins native state, promote their aggregation. However, the mechanisms leading from folded to aggregated states are still incompletely understood. To investigate these mechanisms, we used a combination of NMR spectroscopy and molecular dynamics simulations to compare the native state dynamics of Beta-2 microglobulin (β2m), whose aggregation is associated with dialysis-related amyloidosis, and its aggregation-resistant mutant W60G. Our results indicate that W60G low aggregation propensity can be explained, beyond its higher stability, by an increased average protection of the aggregation-prone residues at its surface. To validate these findings, we designed β2m variants that alter the aggregation-prone exposed surface of wild-type and W60G β2m modifying their aggregation propensity. These results allowed us to pinpoint the role of dynamics in β2m aggregation and to provide a new strategy to tune protein aggregation by modulating the exposure of aggregation-prone residues.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Camilloni C,Sala BM,Sormanni P,Porcari R,Corazza A,De Rosa M,Zanini S,Barbiroli A,Esposito G,Bolognesi M,Bellotti V,Vendruscolo M,Ricagno S

doi

10.1038/srep25559

subject

Has Abstract

pub_date

2016-05-06 00:00:00

pages

25559

issn

2045-2322

pii

srep25559

journal_volume

6

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Relationships of wild and domesticated rices (Oryza AA genome species) based upon whole chloroplast genome sequences.

    abstract：:Rice is the most important crop in the world, acting as the staple food for over half of the world's population. The evolutionary relationships of cultivated rice and its wild relatives have remained contentious and inconclusive. Here we report on the use of whole chloroplast sequences to elucidate the evolutionary an...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep13957

    authors： Wambugu PW,Brozynska M,Furtado A,Waters DL,Henry RJ

    更新日期：2015-09-10 00:00:00

  • Structural and functional characterization of M. tuberculosis sedoheptulose- 7-phosphate isomerase, a critical enzyme involved in lipopolysaccharide biosynthetic pathway.

    abstract：:M. tuberculosis GmhA enzyme catalyzes the isomerization of D-sedoheptulose 7-phosphate into D-glycero-D-α-manno-heptose-7-phosphate in GDP-D-glycero-α-D-manno-heptose biosynthetic pathway. The D-glycero-α-D-manno-heptose is a major constituent of lipopolysaccharide and contributes to virulence and antibiotic resistanc...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-77230-8

    authors： Karan S,Pratap B,Yadav SP,Ashish F,Saxena AK

    更新日期：2020-11-30 00:00:00

  • Dietary fiber intake and pancreatic cancer risk: a meta-analysis of epidemiologic studies.

    abstract：:Evidence on the association between dietary fiber intake and pancreatic cancer risk has been controversial. Therefore, we carried out this meta-analysis to summarize available evidence from epidemiologic studies on this point. Relevant studies were identified by searching PubMed, Embase and Web of Science databases as...

    journal_title：Scientific reports

    pub_type： 杂志文章,meta分析

    doi：10.1038/srep10834

    authors： Wang CH,Qiao C,Wang RC,Zhou WP

    更新日期：2015-06-02 00:00:00

  • Author Correction: The role of directional interactions in the designability of generalized heteropolymers.

    abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-018-22649-3

    authors： Cardelli C,Bianco V,Rovigatti L,Nerattini F,Tubiana L,Dellago C,Coluzza I

    更新日期：2018-03-12 00:00:00

  • к Opioids inhibit tumor angiogenesis by suppressing VEGF signaling.

    abstract：:Opioids are effective analgesics for the management of moderate to severe cancer pain. Here we show that κ opioid receptor (KOR) agonists act as anti-angiogenic factors in tumors. Treatment with KOR agonists, U50,488H and TRK820, significantly inhibited human umbilical vein endothelial cell (HUVEC) migration and tube ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep03213

    authors： Yamamizu K,Furuta S,Hamada Y,Yamashita A,Kuzumaki N,Narita M,Doi K,Katayama S,Nagase H,Yamashita JK,Narita M

    更新日期：2013-11-14 00:00:00

  • Novel function of LHFPL2 in female and male distal reproductive tract development.

    abstract：:Congenital reproductive tract anomalies could impair fertility. Female and male reproductive tracts are developed from Müllerian ducts and Wolffian ducts, respectively, involving initiation, elongation and differentiation. Genetic basis solely for distal reproductive tract development is largely unknown. Lhfpl2 (lipom...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep23037

    authors： Zhao F,Zhou J,Li R,Dudley EA,Ye X

    更新日期：2016-03-11 00:00:00

  • Effect of acute exercise on RBC deformability and RBC nitric oxide synthase signalling pathway in young sickle cell anaemia patients.

    abstract：:Sickle cell anaemia (SCA) is characterized by reduced red blood cell (RBC) deformability and nitric oxide (NO) bioavailability. The aim of the study was to investigate whether exercise might affect these parameters in SCA. SCA patients and healthy controls (AA) performed an acute submaximal exercise test until subject...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-48364-1

    authors： Grau M,Jerke M,Nader E,Schenk A,Renoux C,Collins B,Dietz T,Bizjak DA,Joly P,Bloch W,Connes P,Prokop A

    更新日期：2019-08-14 00:00:00

  • Characterization of cereblon-dependent targeted protein degrader by visualizing the spatiotemporal ternary complex formation in cells.

    abstract：:Targeted protein degradation (TPD) through a proteasome-dependent pathway induced by heterofunctional small molecules is initiated by the formation of a ternary complex with recruited E3 ligases. This complex formation affects the degradation ability of TPD molecules, and thus we tested for visualization of the intrac...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-59966-5

    authors： Kaji T,Koga H,Kuroha M,Akimoto T,Hayata K

    更新日期：2020-02-20 00:00:00

  • Experimental controlled-NOT gate simulation with thermal light.

    abstract：:We report a recent experimental simulation of a controlled-NOT gate operation based on polarization correlation measurements of thermal fields in photon-number fluctuations. The interference between pairs of correlated paths at the very heart of these experiments has the potential for the simulation of correlations be...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep30152

    authors： Peng T,Tamma V,Shih Y

    更新日期：2016-07-21 00:00:00

  • Type 2 Innate Lymphocytes Actuate Immunity Against Tumours and Limit Cancer Metastasis.

    abstract：:Type 2 innate lymphoid cells (ILC2) potentiate immune responses, however, their role in mediating adaptive immunity in cancer has not been assessed. Here, we report that mice genetically lacking ILC2s have significantly increased tumour growth rates and conspicuously higher frequency of circulating tumour cells (CTCs)...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-20608-6

    authors： Saranchova I,Han J,Zaman R,Arora H,Huang H,Fenninger F,Choi KB,Munro L,Pfeifer CG,Welch I,Takei F,Jefferies WA

    更新日期：2018-02-13 00:00:00

  • Chemokine receptor 7 overexpression promotes mesenchymal stem cell migration and proliferation via secreting Chemokine ligand 12.

    abstract：:Great interest has been shown in mesenchymal stem cell (MSC) therapy in a wide variety of clinical domains. However, the therapeutic efficiency depends on the proliferation and migration of MSCs. Chemokine receptors are involved in regulating the proliferation and migration to the specific organs of MSCs in different ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-18509-1

    authors： Liu L,Chen JX,Zhang XW,Sun Q,Yang L,Liu A,Hu S,Guo F,Liu S,Huang Y,Yang Y,Qiu HB

    更新日期：2018-01-09 00:00:00

  • Cell population heterogeneity driven by stochastic partition and growth optimality.

    abstract：:A fundamental question in biology is how cell populations evolve into different subtypes based on homogeneous processes at the single cell level. Here we show that population bimodality can emerge even when biological processes are homogenous at the cell level and the environment is kept constant. Our model is based o...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-45882-w

    authors： Fernandez-de-Cossio-Diaz J,Mulet R,Vazquez A

    更新日期：2019-06-28 00:00:00

  • Mechanosensitive physiology of Chlamydomonas reinhardtii under direct membrane distortion.

    abstract：:Cellular membrane distortion invokes variations in cellular physiology. However, lack of an appropriate system to control the stress and facilitate molecular analyses has hampered progress of relevant studies. In this study, a microfluidic system that finely manipulates membrane distortion of Chlamydomonas reinhardtii...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep04675

    authors： Min SK,Yoon GH,Joo JH,Sim SJ,Shin HS

    更新日期：2014-04-14 00:00:00

  • Giant photoluminescence emission in crystalline faceted Si grains.

    abstract：:Empowering an indirect band-gap material like Si with optical functionalities, firstly light emission, represents a huge advancement constantly pursued in the realization of any integrated photonic device. We report the demonstration of giant photoluminescence (PL) emission by a newly synthesized material consisting o...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep02674

    authors： Faraci G,Pennisi AR,Alberti A,Ruggeri R,Mannino G

    更新日期：2013-01-01 00:00:00

  • Novel Model of Tendon Regeneration Reveals Distinct Cell Mechanisms Underlying Regenerative and Fibrotic Tendon Healing.

    abstract：:To date, the cell and molecular mechanisms regulating tendon healing are poorly understood. Here, we establish a novel model of tendon regeneration using neonatal mice and show that neonates heal via formation of a 'neo-tendon' that differentiates along the tendon specific lineage with functional restoration of gait a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep45238

    authors： Howell K,Chien C,Bell R,Laudier D,Tufa SF,Keene DR,Andarawis-Puri N,Huang AH

    更新日期：2017-03-23 00:00:00

  • Loss of Gαi proteins impairs thymocyte development, disrupts T-cell trafficking, and leads to an expanded population of splenic CD4+PD-1+CXCR5+/- T-cells.

    abstract：:Thymocyte and T cell trafficking relies on signals initiated by G-protein coupled receptors. To address the importance of the G-proteins Gαi2 and Gαi3 in thymocyte and T cell function, we developed several mouse models. Gαi2 deficiency in hematopoietic progenitors led to a small thymus, a double negative (DN)1/DN2 thy...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-04537-4

    authors： Hwang IY,Harrison K,Park C,Kehrl JH

    更新日期：2017-06-23 00:00:00

  • G6Pase location in the endoplasmic reticulum: Implications on compartmental analysis of FDG uptake in cancer cells.

    abstract：:The favourable kinetics of 18F-fluoro-2-deoxyglucose (FDG) permits to depict cancer glucose consumption by a single evaluation of late tracer uptake. This standard procedure relies on the slow radioactivity loss, usually attributed to the limited tumour expression of G6P-phosphatase (G6Pase). However, this classical i...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-38973-1

    authors： Scussolini M,Bauckneht M,Cossu V,Bruno S,Orengo AM,Piccioli P,Capitanio S,Yosifov N,Ravera S,Morbelli S,Piana M,Sambuceti G,Caviglia G,Marini C

    更新日期：2019-02-26 00:00:00

  • Predicting malignant progression in clinically high-risk lesions by DNA ploidy analysis and dysplasia grading.

    abstract：:The value of image cytometry DNA ploidy analysis and dysplasia grading to predict malignant transformation has been determined in oral lesions considered to be at 'high' risk on the basis of clinical information and biopsy result. 10-year follow up data for 259 sequential patients with oral lesions clinically at 'high...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-34165-5

    authors： Zaini ZM,McParland H,Møller H,Husband K,Odell EW

    更新日期：2018-10-26 00:00:00

  • Conformational changes influence clogging behavior of micrometer-sized microgels in idealized multiple constrictions.

    abstract：:Clogging of porous media by soft particles has become a subject of extensive research in the last years and the understanding of the clogging mechanisms is of great importance for process optimization. The rise in the utilization of microfluidic devices brought the possibility to simulate membrane filtration and perfo...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-45791-y

    authors： Bouhid de Aguiar I,Meireles M,Bouchoux A,Schroën K

    更新日期：2019-06-25 00:00:00

  • Infant-directed speech facilitates seven-month-old infants' cortical tracking of speech.

    abstract：:This study assessed cortical tracking of temporal information in incoming natural speech in seven-month-old infants. Cortical tracking refers to the process by which neural activity follows the dynamic patterns of the speech input. In adults, it has been shown to involve attentional mechanisms and to facilitate effect...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-32150-6

    authors： Kalashnikova M,Peter V,Di Liberto GM,Lalor EC,Burnham D

    更新日期：2018-09-13 00:00:00

  • Sum uncertainty relations for arbitrary N incompatible observables.

    abstract：:We formulate uncertainty relations for arbitrary N observables. Two uncertainty inequalities are presented in terms of the sum of variances and standard deviations, respectively. The lower bounds of the corresponding sum uncertainty relations are explicitly derived. These bounds are shown to be tighter than the ones s...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep14238

    authors： Chen B,Fei SM

    更新日期：2015-09-15 00:00:00

  • Historical colonization and dispersal limitation supplement climate and topography in shaping species richness of African lizards (Reptilia: Agaminae).

    abstract：:To what extent deep-time dispersal limitation shapes present-day biodiversity at broad spatial scales remains elusive. Here, we compiled a continental dataset on the distributions of African lizard species in the reptile subfamily Agaminae (a relatively young, Neogene radiation of agamid lizards which ancestors coloni...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep34014

    authors： Kissling WD,Blach-Overgaard A,Zwaan RE,Wagner P

    更新日期：2016-09-27 00:00:00

  • Bifunctional Mesoporous Carbon Nitride: Highly Efficient Enzyme-like Catalyst for One-pot Deacetalization-Knoevenagel Reaction.

    abstract：:Recently, mesoporous carbon nitride (MCN) has aroused extensive interest for its potential applications in organocatalysis, photo- and electrochemistry and CO2 capture. However, further surface functionalization of MCN for advanced nanomaterials and catalysis still remains very challenging. Here we show that acidic ca...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep12901

    authors： Zhong L,Anand C,Lakhi KS,Lawrence G,Vinu A

    更新日期：2015-08-05 00:00:00

  • A Single Dose of Modified Vaccinia Ankara expressing Ebola Virus Like Particles Protects Nonhuman Primates from Lethal Ebola Virus Challenge.

    abstract：:Ebola virus (EBOV), isolate Makona, was the causative agent of the West African epidemic devastating predominantly Guinea, Liberia and Sierra Leone from 2013-2016. While several experimental vaccine and treatment approaches have been accelerated through human clinical trials, there is still no approved countermeasure ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-19041-y

    authors： Domi A,Feldmann F,Basu R,McCurley N,Shifflett K,Emanuel J,Hellerstein MS,Guirakhoo F,Orlandi C,Flinko R,Lewis GK,Hanley PW,Feldmann H,Robinson HL,Marzi A

    更新日期：2018-01-16 00:00:00

  • Accuracy for diagnosis of periapical cystic lesions.

    abstract：:Clinical differentiation between cystic lesions of endodontic and non-endodontic origin is of importance because correct diagnosis may affect treatment decision making. Most radicular cysts are treated with conservative approaches and, therefore, are not surgically removed. The objective of this study was to determine...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-71029-3

    authors： Tsesis I,Krepel G,Koren T,Rosen E,Kfir A

    更新日期：2020-08-25 00:00:00

  • Hypothermic Oxygenated Machine Perfusion of Liver Grafts from Brain-Dead Donors.

    abstract：:Hypothermic oxygenated machine perfusion (HOPE) was introduced in liver transplantation (LT) to mitigate ischemia-reperfusion injury. Available clinical data mainly concern LT with donors after circulatory-determined death, whereas data on brain-dead donors (DBD) are scarce. To assess the impact of end-ischemic HOPE i...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-45843-3

    authors： Patrono D,Surra A,Catalano G,Rizza G,Berchialla P,Martini S,Tandoi F,Lupo F,Mirabella S,Stratta C,Salizzoni M,Romagnoli R

    更新日期：2019-06-27 00:00:00

  • Hormone therapy at early post-menopause increases cognitive control-related prefrontal activity.

    abstract：:Clinical data have been equivocal and controversial as to the benefits to the brain and cognition of hormone therapy (HT) in postmenopausal women. Recent reevaluation of the role of estrogens proposed that HT may effectively prevent the deleterious effects of aging on cognition, and reduces the risks of dementia, incl...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep44917

    authors： Girard R,Météreau E,Thomas J,Pugeat M,Qu C,Dreher JC

    更新日期：2017-03-21 00:00:00

  • Body sway reflects joint emotional expression in music ensemble performance.

    abstract：:Joint action is essential in daily life, as humans often must coordinate with others to accomplish shared goals. Previous studies have mainly focused on sensorimotor aspects of joint action, with measurements reflecting event-to-event precision of interpersonal sensorimotor coordination (e.g., tapping). However, while...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-36358-4

    authors： Chang A,Kragness HE,Livingstone SR,Bosnyak DJ,Trainor LJ

    更新日期：2019-01-18 00:00:00

  • Fossilized Melts in Mantle Wedge Peridotites.

    abstract：:The shallow oxidized asthenosphere may contain a small fraction of potassic silicate melts that are enriched in incompatible trace elements and volatiles. Here, to determine the chemical composition of such melt, we analysed fossilized melt inclusions, preserved as multiphase solid inclusions, from an orogenic garnet ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-28264-6

    authors： Naemura K,Hirajima T,Svojtka M,Shimizu I,Iizuka T

    更新日期：2018-07-04 00:00:00

  • Microsomal Prostaglandin E Synthase-1 Plays a Critical Role in Long-term Motility Dysfunction after Bowel Obstruction.

    abstract：:Motility dysfunction is present not only during bowel obstruction (BO), but after obstruction is resolved. Previous studies found that lumen distension associated mechano-transcription of COX-2 and production of PGE2 in gut smooth muscle cells (SMC) account for motility dysfunction during obstruction. We hypothesized ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-27230-6

    authors： Lin YM,Fu Y,Hegde S,Tang Y,Radhakrishnan R,Shi XZ

    更新日期：2018-06-11 00:00:00