Abstract:
:The mode of action for oncolytic viruses (OVs) in cancer treatment is thought to depend on a direct initial cytotoxic effect against infected tumor cells and subsequent activation of immune cell responses directed against the neoplasm. To study both of these effects in a mouse model of glioblastoma (GBM), we employed murine GBM cells engineered to constitutively express the type I Herpes Simplex Virus (HSV1) HSV-1 receptor, nectin-1, to allow for more efficient infection and replication by oncolytic HSV (oHSV). These cells were further engineered with a surrogate tumor antigen to facilitate assays of T cell activity. We utilized MRI-based volumetrics to measure GBM responses after injection with the oHSV and bioluminescent imaging (BLI) to determine oHSV replicative kinetics in the injected tumor mass. We found increased infiltration of both surrogate tumor antigen- and oHSV antigen-specific CD8+ T cells within 7 days after oHSV injection. There was no increase in tumor infiltrating CD8+ T cells expressing "exhaustion" markers, yet oHSV infection led to a reduction in PD-1+ CD8+ T cells in injected GBMs and an increase in IFNγ+ CD8+ T cells. There was a significant direct correlation between oHSV-mediated reduction in GBM volume and increased infiltration of both viral and tumor antigen-specific CD8+ T cells, as well as oHSV intratumoral gene activity. These findings imply that CD8+ T cell cytotoxicity against both tumor and viral antigens as well as intratumoral oHSV gene expression are important in oHSV-mediated GBM therapy.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Alayo QA,Ito H,Passaro C,Zdioruk M,Mahmoud AB,Grauwet K,Zhang X,Lawler SE,Reardon DA,Goins WF,Fernandez S,Chiocca EA,Nakashima Hdoi
10.1038/s41598-020-61736-2subject
Has Abstractpub_date
2020-03-20 00:00:00pages
5095issue
1issn
2045-2322pii
10.1038/s41598-020-61736-2journal_volume
10pub_type
杂志文章abstract::The association between alcohol consumption and kidney function is intriguing, but study results are mixed and controversial. We examined the association of alcohol consumption with the overall change in kidney function over 12 years. We analyzed data from a population-based cohort that was part of the Korean Genome a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81777-5
更新日期:2021-01-27 00:00:00
abstract::Vitiligo is an acquired depigmentation disorder largely caused by defective melanocyte- or autoimmunity-induced melanocyte destruction. The aryl hydrocarbon receptor (AHR) is essential for melanocyte homeostasis and immune process, and abnormal AHR was observed in vitiligo. We previously identified the T allele of AHR...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13542
更新日期:2015-09-15 00:00:00
abstract::Sixty norovirus outbreaks that occurred in Pudong District, Shanghai in 2017 and affected 959 people were summarised. Of the outbreaks, 29 (48.3%), 27 (45.0%), and 4 (6.7%) occurred in kindergartens, primary schools, and middle schools, respectively. Although the total number of outbreaks peaked in March (13/60, 21.7%...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33724-0
更新日期:2018-10-15 00:00:00
abstract::Signatures of spatially varying selection have been investigated both at the genomic and transcriptomic level in several organisms. In Drosophila melanogaster, the majority of these studies have analyzed North American and Australian populations, leading to the identification of several loci and traits under selection...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34267-0
更新日期:2018-11-01 00:00:00
abstract::Patients who survive influenza A (H7N9) virus infection are at risk of physical and psychological complications of lung injury and multi-organ dysfunction. However, there were no prospectively individualized assessments of physiological, functional and quality-of-life measures after hospital discharge. The current stu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17497-6
更新日期:2017-12-08 00:00:00
abstract::Colour polymorphism may be maintained within a population by disruptive-selection. One hypothesis proposes that different morphs are adapted to different ambient light conditions, with lighter morphs having a selective advantage in bright conditions and darker morphs having advantages in darker conditions. The mechani...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07829-x
更新日期:2017-08-22 00:00:00
abstract::This study compares the effects of temperature (constant at 15, 20, 25, 30 and 35 °C) on adult longevity, oviposition, and nymph development of the brown planthopper, Nilaparvata lugens, on susceptible and resistant rice varieties. The resistant variety contained the BPH32 gene. In our experiments, nymphs failed to de...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80704-4
更新日期:2021-01-08 00:00:00
abstract::Brain metastasis is a significant unmet clinical problem in breast cancer treatment. It is always associated with poor prognosis and high morbidity. Recently, Slit2/Robo1 pathway has been demonstrated to be involved in the progression of breast carcinoma. However, until present, there are no convincing reports that su...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14430
更新日期:2015-09-24 00:00:00
abstract::Systemic Lupus Erythematosus (SLE) is the prototype of autoimmune diseases, characterized by extensive gene expression perturbations in peripheral blood immune cells. Circumstantial evidence suggests that these perturbations may be due to altered epigenetic profiles and chromatin accessibility but the relationship bet...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73654-4
更新日期:2020-10-06 00:00:00
abstract::In spite of much work on topological insulators (TIs), systematic experiments for TI/TI heterostructures remain absent. We grow a high quality heterostructure containing single quintuple layer (QL) of Bi2Se3 on 19 QLs of Bi2Te3 and compare its transport properties with 20 QLs Bi2Se3 and 20 QLs Bi2Te3. All three films ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03060
更新日期:2013-10-28 00:00:00
abstract::Esophageal and gastric cancers share some risk factors. This study aimed to compare the long-term trends in mortality rates of esophageal and gastric cancers in China to provide evidence for cancer prevention and control. Mortality data were derived from 103 continuous points of the Disease Surveillance Points system ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07071-5
更新日期:2017-07-28 00:00:00
abstract::The sensory neurons in the olfactory epithelium (OSNs) are equipped with a large repertoire of olfactory receptors and the associated signal transduction machinery. In addition to the canonical OSNs, which express odorant receptors (ORs), the epithelium contains specialized subpopulations of sensory neurons that can d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25507
更新日期:2016-05-05 00:00:00
abstract::TMEM230 mutations have been recently reported to cause autosomal dominant Parkinson's disease (PD). However, there are limited studies from different ethnic populations to support the role of TMEM230 in sporadic PD. In this study, we performed a comprehensive TMEM230 mutation screening in 550 sporadic PD patients and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01398-9
更新日期:2017-04-26 00:00:00
abstract::The synthetic cyclic hexapeptide cWFW (cyclo(RRRWFW)) has a rapid bactericidal activity against both Gram-positive and Gram-negative bacteria. Its detailed mode of action has, however, remained elusive. In contrast to most antimicrobial peptides, cWFW neither permeabilizes the membrane nor translocates to the cytoplas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44332
更新日期:2017-03-09 00:00:00
abstract::A long-lived reservoir of latently infected T cells prevents antiretroviral therapy from eliminating HIV-1 infection. Furthering our understanding of the dynamics of latency generation and maintenance is therefore vital to improve treatment outcome. Using mathematical models and experiments, we suggest that the death ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04130-9
更新日期:2017-06-19 00:00:00
abstract::Glacial refugia are centers of high biodiversity. Therefore, knowledge on their locations and reactions of associated populations and landscapes to climatic changes is crucial for conservation management. We here investigated the biogeography of a butterfly species linked to open forest habitats. Using mitochondrial a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79376-x
更新日期:2021-01-08 00:00:00
abstract::Freeze-thaw cycles are predicted to increase in cold temperate regions. The potential influence of the interactions of freeze-thaw cycles and agrochemicals on the release of Cd into river water is unknown. In this study, the interactions of freeze-thaw cycles and chlorpyrifos (FC) on Cd mobility in soils were analysed...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27302
更新日期:2016-06-02 00:00:00
abstract::The genetic sexing of animals having long gestation periods offers significant benefits in regard to breeding management among their populations living in captivity. In our study, a new increased-sensitivity PCR method for fetal sexing was developed and tested successfully on elephants, from only a small volume of mat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51641-8
更新日期:2019-10-24 00:00:00
abstract::Soybean is an important source of protein, oil and carbohydrates, as well as other beneficial nutrients. A major function of proteins in nutrition is to supply adequate amounts of amino acids. Although they are essential for human nutrition, the sulfur-containing amino acids cysteine (Cys) and methionine (Met) are oft...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78907-w
更新日期:2020-12-11 00:00:00
abstract::We aimed to predict molecular subtypes of breast cancer using radiomics signatures extracted from synthetic mammography reconstructed from digital breast tomosynthesis (DBT). A total of 365 patients with invasive breast cancer with three different molecular subtypes (luminal A + B, luminal; HER2-positive, HER2; triple...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78681-9
更新日期:2020-12-09 00:00:00
abstract::Interleukin-36 (IL-36) represents three cytokines, IL-36α, IL-36β and IL-36γ, which bind to the same receptor, IL-1RL2; however, their physiological function(s) remain poorly understood. Here, the role of IL-36 in immunity against HSV-1 was examined using the flank skin infection mouse model. Expression analyses revea...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05363-4
更新日期:2017-07-19 00:00:00
abstract::We identify a new instability in electrostatic actuators dubbed quasi-static pull-in. We report experimental evidence of the instability and study its characteristics in two types of micro actuators operating in ambient air. We found that the underlying mechanism is a fast-slow dynamic interaction between slowly-varyi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61534-w
更新日期:2020-03-19 00:00:00
abstract::The synthetic Angiotensin Converting Enzyme (ACE) inhibitors have side effects and hence demands for natural ACE inhibitors have been rising. The aim of this study is to purify and introduce natural ACE inhibitors extracted from Zizyphus jujuba fruits. Proteins from Zizyphus jujuba were lysed by trypsin, papain and th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60972-w
更新日期:2020-03-04 00:00:00
abstract::With countless biological details emerging from cancer experiments, there is a growing need for minimal mathematical models which simultaneously advance our understanding of single tumors and metastasis, provide patient-personalized predictions, whilst avoiding excessive hard-to-measure input parameters which complica...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00031
更新日期:2011-01-01 00:00:00
abstract::Cold-inducible RNA-binding protein (CIRP), released into the circulation during sepsis, causes lung injury via an as yet unknown mechanism. Since endoplasmic reticulum (ER) stress is associated with acute lung injury (ALI), we hypothesized that CIRP causes ALI via induction of ER stress. To test this hypothesis, we st...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41363
更新日期:2017-01-27 00:00:00
abstract::Microscopic plastic (microplastic, 0.1 µm-5 mm) is a widespread pollutant impacting upon aquatic ecosystems across the globe. Environmental sampling has revealed synthetic fibers are prevalent in seawater, sediments and biota. However, microplastic fibers are rarely used in laboratory studies as they are unavailable f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34519
更新日期:2016-10-03 00:00:00
abstract::Venous thromboembolism (VTE) is an important postoperative complication. We investigated and analyzed the current inguinal hernia treatment methods and occurrence of early postoperative VTE in Chinese adults. This study involved data for patients with inguinal hernia hospitalized in 58 general hospitals in mainland Ch...
journal_title:Scientific reports
pub_type: 杂志文章,多中心研究
doi:10.1038/s41598-020-69453-6
更新日期:2020-07-29 00:00:00
abstract::Alterations of carbohydrate structures in cancer cells are the most promising targets for developing clinical diagnostic reagents. Pancreatic cancer is one of the most difficult cancers to diagnose because it lacks definitive symptoms. Two antibodies were raised against human pancreatic ribonuclease 1 that bind to the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06715
更新日期:2014-10-22 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-35358-8
更新日期:2018-11-16 00:00:00
abstract::The Vavilov Institute of Plant Genetic Resources (VIR), in St. Petersburg, Russia, houses a unique genebank, with historical collections of landraces. When they were collected, the geographical distribution and genetic diversity of most crops closely reflected their historical patterns of cultivation established over ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05087-5
更新日期:2017-07-06 00:00:00