Abstract:
:The genetic sexing of animals having long gestation periods offers significant benefits in regard to breeding management among their populations living in captivity. In our study, a new increased-sensitivity PCR method for fetal sexing was developed and tested successfully on elephants, from only a small volume of maternal plasma. Suitable sensitivity was obtained by using short, reduced amplicon lengths with fluorescent labelling for capillary electrophoresis detection. The fundamental principle for this technique was based on the detection of two Y-specific markers (AmelY and SRY), the presence of which indicates the mother is carrying a male fetus and the absence of these markers designates a female fetus. As a reaction control, the X-chromosomal marker (PlpX) was used. To the best of our knowledge, this is the first report on this topic, confirming the presence of fetal cell-free DNA from the plasma of a pregnant captive elephant, and demonstrating a new opportunity for non-invasive assessment in fetal sex determination.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Vincze B,Gáspárdy A,Biácsi A,Papp EÁ,Garamvölgyi L,Sós E,Cseh S,Kovács G,Pádár Z,Zenke Pdoi
10.1038/s41598-019-51641-8subject
Has Abstractpub_date
2019-10-24 00:00:00pages
15254issue
1issn
2045-2322pii
10.1038/s41598-019-51641-8journal_volume
9pub_type
杂志文章abstract::Atherosclerosis is a progressive disease leading to loss of vascular homeostasis and entails fibrosis, macrophage foam cell formation, and smooth muscle cell proliferation. Recent studies have reported that epidermal growth factor receptor (EGFR) is involved vascular pathophysiology and in the regulation of oxidative ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45917
更新日期:2017-04-04 00:00:00
abstract::Cell lytic enzymes represent an alternative to chemical decontamination or use of antibiotics to kill pathogenic bacteria, such as listeria. A number of phage cell lytic enzymes against listeria have been isolated and possess listericidal activity; however, there has been no attempt to incorporate these enzymes onto s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01584
更新日期:2013-01-01 00:00:00
abstract::Many environmental flow (e-flow) studies and applications have predominantly used state-(i.e., at a single time point) and rate-(i.e., temporal change) based demographic characteristics of species representing lower trophic levels (e.g., fish communities) to build flow-ecology relationships, rather than using a proces...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79532-3
更新日期:2020-12-18 00:00:00
abstract::Ce(3+)-doped yttrium aluminum garnet (YAG:Ce) nanocrystals were successfully synthesized via a facile sol-gel method. Multiple characterization techniques were employed to study the structure, morphology, composition and photoluminescence properties of YAG:Ce nanophosphors. The YAG:Ce0.0055 sintered at 1030 °C exhibit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22238
更新日期:2016-03-03 00:00:00
abstract::Whole exome sequencing is a technique that aims to selectively sequence all exons of protein-coding genes. A canine whole exome sequencing enrichment kit was designed based on the latest canine reference genome (build 3.1.72). Its performance was tested by sequencing 2 exome captures, each consisting of 4 pre-capture ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05597
更新日期:2014-07-07 00:00:00
abstract::Maximal rate of heart rate (HR) increase (rHRI) as a measure of HR acceleration during the transition from rest to exercise, or during an increase in workload, tracks exercise performance. rHRI assessed at relative rather than absolute workloads may track performance better, and a field test would increase applicabili...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71597-4
更新日期:2020-09-03 00:00:00
abstract::The aim of this study was to identify novel plasma metabolic signatures with possible relevance during multiple myeloma (MM) development and progression. A biochemical quantitative phenotyping platform based on targeted electrospray ionization tandem mass spectrometry technology was used to aid in the identification o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75862-4
更新日期:2020-12-14 00:00:00
abstract::Viruses induce double-stranded RNA (dsRNA) in the host cells. The mammalian system has developed dsRNA-dependent recognition receptors such as RLRs that recognize the long stretches of dsRNA as PAMPs to activate interferon-mediated antiviral pathways and apoptosis in severe infection. Here we report an efficient antiv...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22550
更新日期:2016-03-03 00:00:00
abstract::One-carbon metabolism is essential for multiple cellular processes and can be assessed by the concentration of folate metabolites in the blood. One-carbon metabolites serve as methyl donors that are required for epigenetic regulation. Deficiencies in these metabolites are associated with a variety of poor health outco...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35111-1
更新日期:2018-11-15 00:00:00
abstract::The inter-relationship between subtropical western-central Pacific sea surface temperatures (STWCPSST), sea ice concentrations in the Beaufort Sea (SICBS), and the North Atlantic Oscillation (NAO) in summer are investigated over the period 1980-2016. It is shown that the Arctic response to the remote impact of the Pac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39896-7
更新日期:2019-03-05 00:00:00
abstract::Adapters bind motor proteins to cargoes and therefore play essential roles in Kinesin-1 mediated intracellular transport. The regulatory mechanisms governing adapter functions and the spectrum of cargoes recognized by individual adapters remain poorly defined. Here, we show that cargoes transported by the Kinesin-1 ad...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26965
更新日期:2016-06-01 00:00:00
abstract::Exploiting new, low-cost and efficient electrocatalysts for hydrogen evolution reaction (HER) is important to resolve the energy crisis and environment pollution. In this work, graphene decorated with Ni nanoparticles (NPs) were synthesized via one-pot reduction using graphene oxide (GO, the obtained composite was den...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10589
更新日期:2015-06-12 00:00:00
abstract::Oryza coarctata (KKLL; 2n = 4x = 48, 665 Mb) also known as Porteresia coarctata is an extreme halophyte species of genus Oryza. Using Illumina and Nanopore reads, we achieved the assembled genome size of 569.9 Mb, accounting 85.69% of the estimated genome size with N50 of 1.85 Mb and 19.89% repetitive region. We also ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31518-y
更新日期:2018-09-12 00:00:00
abstract::Pulse wave transit time (PWTT) has been widely used as an index in assessing arterial stiffness. Percutaneous coronary intervention (PCI) is usually applied to the treatment of coronary artery disease (CAD). Research on the changes in PWTT caused by PCI is helpful for understanding the impact of the PCI procedure. In ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-18520-6
更新日期:2018-01-08 00:00:00
abstract::We report the transient memory device by means of a water soluble SSG (solid sodium with glycerine) paper. This material has a hydroscopic property hence it can be soluble in water. In terms of physical security of memory devices, prompt abrogation of a memory device which stored a large number of data is crucial when...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38324
更新日期:2016-12-05 00:00:00
abstract::Attaching Unique Molecular Identifiers (UMI) to RNA molecules in the first step of sequencing library preparation establishes a distinct identity for each input molecule. This makes it possible to eliminate the effects of PCR amplification bias, which is particularly important where many PCR cycles are required, for e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31064-7
更新日期:2018-09-03 00:00:00
abstract::The influence of the growth conditions of multilayer CdTe/ZnTe quantum dots (QDs) on Si substrate upon their carrier dynamics is studied using intensity integration and broadening photoluminescence. The unusual temperature dependence of the line broadening is explained using a model for interband transitions that invo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08267
更新日期:2015-02-05 00:00:00
abstract::Recent advances in genetic analyses have significantly refined human type 1 diabetes (T1D) associated loci. The goal of such effort is to identify the causal genes and have a complete understanding of the molecular pathways that independently or interactively influence cellular processes leading to the destruction of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68956-6
更新日期:2020-07-21 00:00:00
abstract::Coastal wetlands are important carbon sinks globally, but their ability to store carbon hinges on their nitrogen (N) supply and N uptake dynamics of dominant plant species. In terrestrial ecosystems, uptake of nitrate (NO3-) and ammonium (NH4+) through roots can strongly influence N acquisition rates and their respons...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23349-8
更新日期:2018-03-29 00:00:00
abstract::Cartilage engineering that combines competent seeding cells and a compatible scaffold is increasingly gaining popularity and is potentially useful for the treatment of various bone and cartilage diseases. Intensive efforts have been made by researchers to improve the viability and functionality of seeding cells of eng...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26617
更新日期:2016-05-25 00:00:00
abstract::Mandrill (Mandrillus sphinx) is a primate species, which belongs to the Old World monkey (Cercopithecidae) family. It is closely related to human, serving as a model for human health related research. However, the genetic studies on and genomic resources of mandrill are limited, especially in comparison to other prima...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59110-3
更新日期:2020-02-12 00:00:00
abstract::Visual information can alter auditory perception. This is clearly illustrated by the well-known McGurk illusion, where an auditory/aba/ and a visual /aga/ are merged to the percept of 'ada'. It is less clear however whether such a change in perception may recalibrate subsequent perception. Here we asked whether the al...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32891
更新日期:2016-09-09 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-75277-1
更新日期:2020-10-20 00:00:00
abstract::Kefir peptides, generated by kefir grain fermentation of milk proteins, showed positive antioxidant effects, lowered blood pressure and modulated the immune response. In this study, kefir peptide was evaluated regarding their anti-inflammatory effects on particulate matter <4 μm (PM4.0)-induced lung inflammation in NF...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47872-4
更新日期:2019-08-08 00:00:00
abstract::In absence of soil erosion plots for determination of erodibility index (K) for erosion models like Universal Soil Loss Equation (USLE) or Revised Universal Soil Loss Equation (RUSLE) to estimate soil erosion, empirical relations are used. In the present study, soil erodibility index was determined for entire Ri-bhoi ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72070-y
更新日期:2020-09-11 00:00:00
abstract::Myocardial infarction (MI) can result in sympathetic nerve loss in the infarct region. However, the contribution of hypo-innervation to electrophysiological remodeling, independent from MI-induced ischemia and fibrosis, has not been comprehensively investigated. We present a novel mouse model of regional cardiac sympa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75903-y
更新日期:2020-11-02 00:00:00
abstract::Ankle arthrodesis with the Ilizarov method is an accepted form of treatment of advanced degenerative changes of the ankle joint. Incorrect balance and load distribution on the lower limbs may result in pain and dysfunction. The aim of the study was to assess the change of balance and load distribution in lower extremi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34016-3
更新日期:2018-10-24 00:00:00
abstract::We study localized photonic excitations in a quasi-two-dimensional non-ideal binary microcavity lattice with use of the virtual crystal approximation. The effect of point defects (vacancies) on the excitation spectrum is investigated by numerical modelling. We obtain the dispersion and the energy gap of the electromag...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06945
更新日期:2014-11-06 00:00:00
abstract::Previously, we cloned a new gene termed 'tongue cancer resistance-associated protein 1' (TCRP1), which modulates tumorigenesis, enhances cisplatin (cDDP) resistance in cancers, and may be a potential target for reversing drug resistance. However, the mechanisms for regulating TCRP1 expression remain unclear. Herein, w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03763-0
更新日期:2017-06-16 00:00:00
abstract::Molybdenum disulfide (MoS2) has been attracting much attentions due to its excellent electrical and optical properties. We report here the synthesis of large-scale and uniform MoS2 nanosheets with vertically standing morphology using chemical vapor deposition method. TEM observations clearly reveal the growth mechanis...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21171
更新日期:2016-02-18 00:00:00