Abstract：
:Enzymatic digestion for protein sequencing usually requires much time, and does not always result in high sequence coverage. Here we report the use of aqueous microdroplets to accelerate enzymatic reactions and, in particular, to improve protein sequencing. When a room temperature aqueous solution containing 10 µM myoglobin and 5 µg mL-1 trypsin is electrosonically sprayed (-3 kV) from a homemade setup to produce tiny (∼9 µm) microdroplets, we obtain 100% sequence coverage in less than 1 ms of digestion time, in sharp contrast to 60% coverage achieved by incubating the same solution at 37 °C for 14 h followed by analysis with a commercial electrospray ionization source that produces larger (∼60 µm) droplets. We also confirm the sequence of the therapeutic antibody trastuzumab (∼148 kDa), with a sequence coverage of 100% for light chains and 85% for heavy chains, demonstrating the practical utility of microdroplets in drug development.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Zhong X,Chen H,Zare RNdoi
10.1038/s41467-020-14877-xsubject
Has Abstractpub_date
2020-02-26 00:00:00pages
1049issue
1issn
2041-1723pii
10.1038/s41467-020-14877-xjournal_volume
11pub_type
杂志文章abstract：:Myoblast fusion (MF) is required for muscle growth and repair, and its alteration contributes to muscle diseases. The mechanisms governing this process are incompletely understood, and no epigenetic regulator has been previously described. Ash1L is an epigenetic activator belonging to the Trithorax group of proteins a...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-07313-8
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abstract：:Surface-supported isolated atoms in single-atom catalysts (SACs) are usually stabilized by diverse defects. The fabrication of high-metal-loading and thermally stable SACs remains a formidable challenge due to the difficulty of creating high densities of underpinning stable defects. Here we report that isolated Pt ato...
journal_title：Nature communications
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1038/s41467-019-09957-6
更新日期：2019-04-23 00:00:00
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更新日期：2013-01-01 00:00:00
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journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms11231
更新日期：2016-04-07 00:00:00
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pub_type： 杂志文章
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更新日期：2019-08-09 00:00:00
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doi：10.1038/ncomms12317
更新日期：2016-08-09 00:00:00
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doi：10.1038/s41467-019-11620-z
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更新日期：2020-01-23 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
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pub_type： 已发布勘误
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更新日期：2019-11-20 00:00:00
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